10 research outputs found
Voltammetric determination of chlorogenic acid in pharmaceutical products using poly(aminosulfonic acid) modified glassy carbon electrode
In this work, a poly(aminosulfonic acid) modified glassy carbon electrode was fabricated and the electrochemical behavior of chlorogenic acid (CGA) was studied by cyclic voltammetry. Compared with a bare glassy carbon electrode, the modified electrode exhibits excellent catalytic effect on the electrochemical redox of CGA. Utilizing this catalytic effect, a sensitive and selective electrochemical method for the determination of CGA was developed. The analytical parameters were optimized. Under the optimized conditions, the oxidation peak current is linearly proportional to the concentration of CGA in the range from 4.00 Ă 10â7 to 1.20 Ă 10â5 mol/L and the detection limit is 4.00 Ă 10â8 mol/L. Further, the performance of the proposed method has been validated in terms of linearity (r = 0.9995), recovery (96.3â102.8%), reproducibility (RSD ïŒÂ 4.0%, n = 6) and robustness. The developed method has been successfully applied for the determination of CGA in a variety of pharmaceutical products
Electrocatalytic determination of maltol in food products by cyclic voltammetry with a poly(l-phenylalanine) modified electrode
The study of thermal decomposition of 2-bromo-3,3,3-trifluoropropene and its fire-extinguishing mechanism
Environmental regulation and firm innovation: Evidence from National Specially Monitored Firms program in China
Study on the Electrochemical Properties of Kojic Acid at a Poly(glutamic Acid)-Modified Glassy Carbon Electrode and Its Analytical Application
Simultaneous electrocatalytic determination of lead and cadmium ions employing a poly(methylene blue)/graphene modified glassy carbon electrode
Heavy metal-induced glutathione accumulation and its role in heavy metal detoxification in Phanerochaete chrysosporium
Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9
Genome editing by the well-established CRISPR/Cas9 technology has greatly facilitated our understanding of many biological processes. However, a complete whole-genome knockout for any species or model organism has rarely been achieved. Here, we performed a systematic knockout of all the genes (1333) on Chromosome 1 in zebrafish, successfully mutated 1029 genes, and generated 1039 germline-transmissible alleles corresponding to 636 genes. Meanwhile, by high-throughput bioinformatics analysis, we found that sequence features play pivotal roles in effective gRNA targeting at specific genes of interest, while the success rate of gene targeting positively correlates with GC content of the target sites. Moreover, we found that nearly one-fourth of all mutants are related to human diseases, and several representative CRISPR/Cas9-generated mutants are described here. Furthermore, we tried to identify the underlying mechanisms leading to distinct phenotypes between genetic mutants and antisense morpholino-mediated knockdown embryos. Altogether, this work has generated the first chromosome-wide collection of zebrafish genetic mutants by the CRISPR/Cas9 technology, which will serve as a valuable resource for the community, and our bioinformatics analysis also provides some useful guidance to design gene-specific gRNAs for successful gene editing