Reduction of concentrated feed in dairy cows in the Parmigiano Reggiano area: effects on milk production and quality.

A project of technological research, funded by Emilia Romagna Region and aimed to a higher sustainability of dairy chain, involved an experimental trial testing the effect of a reduction of concentrate feed in Frisona cows producing milk for Parmigiano Reggiano Cheese making. Two groups of 15 cows each, with milk production at 9 days in milk (DIM) of 31.7 and 32.1 kg/d, were fed mixed meadow hay ad libitum and a growing amount of concentrate feed up to 15 kg (C group) or 12 kg (T group) at the peak of lactation (about 60 days). Milk production, milk fat and protein were recorded from each cow at 9, 47, 96, 131, 166, and 207 DIM; fatty acid composition of milk was analysed on 5 pooled samples/group at 47, 131, and 207 DIM. Milk collected from the two groups of cows at day 161 and 168 was used for micro cheese making. Milk production and milk fat were unaffected by diet, whereas milk protein was lower in T cows at 166 (3.18 vs 3.39%; p<.01) and 207 (3.17 vs 3.31%; p<.05) DIM. Fatty acid composition of T milk showed at 47 DIM significantly lower % of MUFA and PUFA, and higher % of SFA, resulting in higher SFA/UFA ratio (1.70 vs 1.38; p<.01). Both n-3 and n-6 fatty acids were significantly lower in T milk at 47 DIM (n-3: 1.21 vs 1.44%; n-6: 4.05 vs 4.57%) and at 131 DIM (n-3: 1.18 vs 1.37%: n-6: 3.89 vs 4.83%). CLA levels were similar in the milk of the two groups. The milk collected for cheese making (60 l/group) had similar chemical and bacteriological composition, with the exception of a higher content of thermophilic Lactobacilli in T milk. Rennet coagulation characteristics showed lower curd firmness at 30’ in T milk (a30: 24.80 vs 29.48 mm; p<.05), whereas the other cheese making traits were not significantly different between the two groups. Bacteriological traits of the ripened cheese (60 days) from the two groups were also similar. The results of this field trial indicate that a reduction of 20% of concentrate feed in the diet of high producing cows did not affect milk amount, milk fat and cheese making traits, but caused a partial deterioration in milk protein, fatty acid composition and rennet coagulation. Further research might evaluate the whole lactation, reproductive performances, nutritional and health status. A part of the results of the research was presented at 6th AITeL Congress (Trento, September 20th 2018)

A First Evaluation of a Multi-Modal Learning System to Control Surgical Assistant Robots via Action Segmentation

The next stage for robotics development is to introduce autonomy and cooperation with human agents in tasks that require high levels of precision and/or that exert considerable physical strain. To guarantee the highest possible safety standards, the best approach is to devise a deterministic automaton that performs identically for each operation. Clearly, such approach inevitably fails to adapt itself to changing environments or different human companions. In a surgical scenario, the highest variability happens for the timing of different actions performed within the same phases. This paper presents a cognitive control architecture that uses a multi-modal neural network trained on a cooperative task performed by human surgeons and produces an action segmentation that provides the required timing for actions while maintaining full phase execution control via a deterministic Supervisory Controller and full execution safety by a velocity-constrained Model-Predictive Controller

Community-wide outbreak of haemolytic uraemic syndrome associated with Shiga toxin 2-producing Escherichia coli O26:H11 in southern Italy, summer 2013

In summer 2013, an excess of paediatric cases of haemolytic uraemic syndrome (HUS) in a southern region of Italy prompted the investigation of a community-wide outbreak of Shiga toxin 2-producing Escherichia coli (STEC) O26:H11 infections. Case finding was based on testing patients with HUS or bloody diarrhoea for STEC infection by microbiological and serological methods. A case-control study was conducted to identify the source of the outbreak. STEC O26 infection was identified in 20 children (median age 17 months) with HUS, two of whom reported severe neurological sequelae. No cases in adults were detected. Molecular typing showed that two distinct STEC O26:H11 strains were involved. The case-control study showed an association between STEC O26 infection and consumption of dairy products from two local plants, but not with specific ready-to-eat products. E.coli O26:H11 strains lacking the stx genes were isolated from bulk milk and curd samples, but their PFGE profiles did not match those of the outbreak isolates. This outbreak supports the view that infections with Stx2-producing E. coli O26 in children have a high probability of progressing to HUS and represent an emerging public health problem in Europe

High-resolution aeromagnetic survey of Calabria (Southern Italy)

We present a 1:350,000 high-resolution magnetic anomaly map of Calabria (Southern Italy), obtained by merging the results from two low-altitude aeromagnetic surveys performed in southern and northern Calabria. Magnetic anomalies of Calabria are of low intensity, and mostly range from 11 to –9 nT. Northern Calabria is characterized by positive anomalies in the Tyrrhenian margin (Coastal Chain) that turn into negative values moving eastward in the Sila Massif. Southern Calabria is characterized by slightly positive anomaly values, interrupted by a null magnetic anomaly corridor roughly corresponding to the eastern margin of the Gioia Tauro basin. Finally, anomaly values turn systematically negative in the Messina Straits. Due to the unprecedented resolution (low flying height, spatial sampling along the flight line of ∼5 m and 1–2 km flight line spacing), the new map highlights, in detail, the geometry and setting of the upper crustal features. As Calabria is one of the most seismically active regions in Italy, hit by several high-magnitude earthquakes in recent centuries, the interpretation of this new map will hopefully contribute to new insights into the crustal geological setting, location and dimension of the main seismogenic sources.Published116-1231A. Geomagnetismo e Paleomagnetismo3SR. AMBIENTE - Servizi e ricerca per la SocietàJCR Journa

Technical and Functional Validation of a Teleoperated Multirobots Platform for Minimally Invasive Surgery

Nowadays Robotic assisted Minimally Invasive Surgeries (R-MIS) are the elective procedures for treating highly accurate and scarcely invasive pathologies, thanks to their abil- ity to empower surgeons\u2019 dexterity and skills. The research on new Multi-Robots Surgery (MRS) platform is cardinal to the development of a new SARAS surgical robotic platform, which aims at carrying out autonomously the assistants tasks during R- MIS procedures. In this work, we will present the SARAS MRS platform validation protocol, framed in order to assess: (i) its technical performances in purely dexterity exercises, and (ii) its functional performances. The results obtained show a prototype able to put the users in the condition of accomplishing the tasks requested (both dexterity- and surgical-related), even with rea- sonably lower performances respect to the industrial standard. The main aspects on which further improvements are needed result to be the stability of the end effectors, the depth per- ception and the vision systems, to be enriched with dedicated virtual fixtures. The SARAS\u2019 aim is to reduce the main surgeon\u2019s workload through the automation of assistive tasks which would benefit both surgeons and patients by facilitating the surgery and reducing the operation time

Functional versus nonfunctional rehabilitation in chronic ischemic stroke: evidences from a randomized functional mri study

Motor rehabilitation of stroke survivors may include functional and/or nonfunctional strategy. The present study aimed to compare the effect of these two rehabilitation strategies by means of clinical scales and functional Magnetic Resonance Imaging (fMRI). Twelve hemiparetic chronic stroke patients were selected. Patients were randomly assigned a nonfunctional (NFS) or functional (FS) rehabilitation scheme. Clinical scales (Fugl-Meyer, ARA test, and modified Barthel) and fMRI were applied at four moments: before rehabilitation (P1) and immediately after (P2), 1 month after (P3), and three months after (P4) the end of rehabilitation. The NFS group improved significantly and exclusively their Fugl-Meyer scores at P2, P3, and P4, when compared to P1. On the other hand, the FS group increased significantly in Fugl-Meyer at P2, when compared to P1, and also in their ARA and Barthel scores. fMRI inspection at the individual level revealed that both rehabilitation schemes most often led to decreased activation sparseness, decreased activity of contralesional M1, increased asymmetry of M1 activity to the ipsilesional side, decreased perilesional activity, and decreased SMA activity. Increased M1 asymmetry with rehabilitation was also confirmed by Lateralization Indexes. Our clinical analysis revealed subtle differences between FS and NFS.CNPqCAPESRadiology Division, Department of Internal Medicine, Ribeirao Preto School of Medicine, University of Sao Paulo, 14049-900 Ribeirao Preto, SP, BrazilBrain Institute/Onofre Lopes University Hospital, Federal University of Rio Grande do Norte, 59153-155 Natal, RN, BrazilDepartment of Psychobiology, Federal University of Sao Paulo (UNIFESP), Sao Paulo, SP, BrazilDepartment of Neuroscience and Behavior, Ribeirao Preto School of Medicine, University of Sao Paulo, 14049-900 Ribeirao Preto, SP, BrazilDepartment of Psychobiology, Federal University of Sao Paulo (UNIFESP), Sao Paulo, SP, BrazilWeb of Scienc

Metagenomic Characterization of the Human Intestinal Microbiota in Fecal Samples from STEC-Infected Patients

The human intestinal microbiota is a homeostatic ecosystem with a remarkable impact on human health and the disruption of this equilibrium leads to an increased susceptibility to infection by numerous pathogens. In this study, we used shotgun metagenomic sequencing and two different bioinformatic approaches, based on mapping of the reads onto databases and on the reconstruction of putative draft genomes, to investigate possible changes in the composition of the intestinal microbiota in samples from patients with Shiga Toxin-producing E. coli (STEC) infection compared to healthy and healed controls, collected during an outbreak caused by a STEC O26:H11 infection. Both the bioinformatic procedures used, produced similar result with a good resolution of the taxonomic profiles of the specimens. The stool samples collected from the STEC infected patients showed a lower abundance of the members of Bifidobacteriales and Clostridiales orders in comparison to controls where those microorganisms predominated. These differences seemed to correlate with the STEC infection although a flexion in the relative abundance of the Bifidobacterium genus, part of the Bifidobacteriales order, was observed also in samples from Crohn's disease patients, displaying a STEC-unrelated dysbiosis. The metagenomics also allowed to identify in the STEC positive samples, all the virulence traits present in the genomes of the STEC O26 that caused the outbreak as assessed through isolation of the epidemic strain and whole genome sequencing. The results shown represent a first evidence of the changes occurring in the intestinal microbiota of children in the course of STEC infection and indicate that metagenomics may be a promising tool for the culture-independent clinical diagnosis of the infection

Shiga Toxin–Producing Escherichia coli Infections Associated with Hemolytic Uremic Syndrome, Italy, 1988–2000

The mean annual incidence of hemolytic uremic syndrome in persons <15 years of age in Italy from 1988 to 2000 was 0.28 per 100,000 population. Laboratory investigations showed that Shiga toxin–producing Escherichia coli (STEC) infection occurred in 73.1% of patients. STEC O157 was the most common serotype, but a considerable number of cases were from infections by non-O157 STEC