Tamizaje de salud mental mediante el test M.I.N.I. en estudiantes del ciclo básico de medicina de la Universidad Nacional de Asunción

INTRODUCCIÓN: Los estudiantes de medicina están expuestos a un mayor número de trastornos mentales que los de la población general por las exigencias propias de la carrera, resaltan entre estos trastornos los de ansiedad y depresión cuyos valores son superiores a los encontrados en la población general. La Mini International Neuropsychiatric Interview (MINI) es una entrevista breve y altamente estructurada de los principales trastornos psiquiátricos del CIE-10. MATERIALES Y MÉTODOS: Fue un estudio observacional analítico con corte transversal realizado en la Facultad de Ciencias Médicas de la Universidad Nacional de Asunción (UNA). Las variables epidemiológicas y sociodemográficas fueron recolectadas con un formulario previamente validado. Se evaluó la frecuencia de trastornos de salud mental en los alumnos utilizando el test MINI, validado en español, como método de tamizaje. RESULTADOS: Se evaluó 91 estudiantes, 58 del sexo femenino (63,7%) y 33 de sexo masculino (36,3%); la media de la edad fue de 21 ± 1 años; 35 alumnos (58,2%) tuvieron al menos un trastorno mental. Los diagnósticos más frecuentes fueron: episodio hipomaniaco pasado (21 alumnos; 23,1%), trastorno depresivo actual (21; 23,1%), trastorno de ansiedad generalizada (15; 16,5%) y agorafobia (14; 15,4%). DISCUSIÓN: los problemas de salud mental fueron frecuentes en la población universitaria que estudiamos siendo el episodio hipomaniaco pasado, el episodio depresivo mayor actual, el trastorno de ansiedad generalizada y la agorafobia, los problemas más frecuentes

A High Resolution Genetic Map Anchoring Scaffolds of the Sequenced Watermelon Genome

As part of our ongoing efforts to sequence and map the watermelon (Citrullus spp.) genome, we have constructed a high density genetic linkage map. The map positioned 234 watermelon genome sequence scaffolds (an average size of 1.41 Mb) that cover about 330 Mb and account for 93.5% of the 353 Mb of the assembled genomic sequences of the elite Chinese watermelon line 97103 (Citrullus lanatus var. lanatus). The genetic map was constructed using an F8 population of 103 recombinant inbred lines (RILs). The RILs are derived from a cross between the line 97103 and the United States Plant Introduction (PI) 296341-FR (C. lanatus var. citroides) that contains resistance to fusarium wilt (races 0, 1, and 2). The genetic map consists of eleven linkage groups that include 698 simple sequence repeat (SSR), 219 insertion-deletion (InDel) and 36 structure variation (SV) markers and spans ∼800 cM with a mean marker interval of 0.8 cM. Using fluorescent in situ hybridization (FISH) with 11 BACs that produced chromosome-specifc signals, we have depicted watermelon chromosomes that correspond to the eleven linkage groups constructed in this study. The high resolution genetic map developed here should be a useful platform for the assembly of the watermelon genome, for the development of sequence-based markers used in breeding programs, and for the identification of genes associated with important agricultural traits

A Strawberry KNOX Gene Regulates Leaf, Flower and Meristem Architecture

The KNOTTED-LIKE HOMEODOMAIN (KNOX) genes play a central role in maintenance of the shoot apical meristem. They also contribute to the morphology of simple and compound leaves. In this report we characterize the FaKNOX1 gene from strawberry (Fragaria spp.) and demonstrate its function in trasgenic plants. The FaKNOX1 cDNA was isolated from a cultivated strawberry (F.×ananassa) flower EST library. The sequence is most similar to Class I KNOX genes, and was mapped to linkage group VI of the diploid strawberry genome. Unlike most KNOX genes studied, steady-state transcript levels were highest in flowers and fruits. Transcripts were also detected in emerging leaf primordia and the apical dome. Transgenic strawberry plants suppressing or overexpressing FaKNOX1 exhibited conspicuous changes in plant form. The FaKNOX1 RNAi plants presented a dwarfed phenotype with deeply serrated leaflets and exaggerated petiolules. They also exhibited a high level of cellular disorganization of the shoot apical meristem and leaves. Overexpression of FaKNOX1 caused dwarfed stature with wrinkled leaves. These gain- and loss-of-function assays in strawberry functionally demonstrate the contributions of a KNOX domain protein in a rosaceous species

Fine‐scale empirical data on niche divergence and homeolog expression patterns in an allopolyploid and its diploid progenitor species

Polyploidization is pervasive in plants, but little is known about the niche divergence of wild allopolyploids (species that harbor polyploid genomes originating from different diploid species) relative to their diploid progenitor species and the gene expression patterns that may underlie such ecological divergence. We conducted a fine-scale empirical study on habitat and gene expression of an allopolyploid and its diploid progenitors. We quantified soil properties and light availability of habitats of an allotetraploid Cardamine flexuosa and its diploid progenitors Cardamine amara and Cardamine hirsuta in two seasons. We analyzed expression patterns of genes and homeologs (homeologous gene copies in allopolyploids) using RNA sequencing. We detected niche divergence between the allopolyploid and its diploid progenitors along water availability gradient at a fine scale: the diploids in opposite extremes and the allopolyploid in a broader range between diploids, with limited overlap with diploids at both ends. Most of the genes whose homeolog expression ratio changed among habitats in C. flexuosa varied spatially and temporally. These findings provide empirical evidence for niche divergence between an allopolyploid and its diploid progenitor species at a fine scale and suggest that divergent expression patterns of homeologs in an allopolyploid may underlie its persistence in diverse habitats

Application of Bioengineering Modalities in Vascular Research: Evaluating the Clinical Gain

Using knowledge gained from bioengineering studies, current vascular research focuses on the delineation of the natural history and risk assessment of clinical vascular entities with significant morbidity and mortality, making the development of new, more accurate predictive criteria a great challenge. Additionally, conclusions derived from computational simulation studies have enabled the improvement and modification of many biotechnology products that are used routinely in the treatment of vascular diseases. This review highlights the promising role of the bioengineering applications in the vascular field