Assimilating MODIS and AMSR-E Snow Observations in a Snow Evolution Model

In this paper four simple computationally inexpensive, direct insertion data assimilation schemes are presented, and evaluated, to assimilate Moderate Resolution Imaging Spectroradiometer (MODIS) snow cover, which is a binary observation, and Advanced Microwave Scanning Radiometer for Earth Observing System (EOS) (AMSR-E) snow water equivalent (SWE) observations, which are at a coarser resolution than MODIS, into a numerical snow evolution model. The four schemes are 1) assimilate MODIS snow cover on its own with an arbitrary 0.01 m added to the model cells if there is a difference in snow cover; 2) iteratively change the model SWE values to match the AMSR-E equivalent value; 3) AMSR-E scheme with MODIS observations constraining which cells can be changed, when both sets of observations are available; and 4) MODIS-only scheme when the AMSR-E observations are not available, otherwise scheme 3. These schemes are used in the winter of 2006/07 over the southeast corner of Colorado and the tri-state area: Wyoming, Colorado, and Nebraska. It is shown that the inclusion of MODIS data enables the model in the north domain to have a 15% improvement in number of days with a less than 10% disagreement with the MODIS observation 24 h later and approximately 5% for the south domain. It is shown that the AMSR-E scheme has more of an impact in the south domain than the north domain. The assimilation results are also compared to station snow-depth data in both domains, where there is up-to-a-factor-of-5 underestimation of snow depth by the assimilation schemes compared with the station data but the snow evolution is fairly consistent

A Bistable Switch and Anatomical Site Control Vibrio cholerae Virulence Gene Expression in the Intestine

A fundamental, but unanswered question in host-pathogen interactions is the timing, localization and population distribution of virulence gene expression during infection. Here, microarray and in situ single cell expression methods were used to study Vibrio cholerae growth and virulence gene expression during infection of the rabbit ligated ileal loop model of cholera. Genes encoding the toxin-coregulated pilus (TCP) and cholera toxin (CT) were powerfully expressed early in the infectious process in bacteria adjacent to epithelial surfaces. Increased growth was found to co-localize with virulence gene expression. Significant heterogeneity in the expression of tcpA, the repeating subunit of TCP, was observed late in the infectious process. The expression of tcpA, studied in single cells in a homogeneous medium, demonstrated unimodal induction of tcpA after addition of bicarbonate, a chemical inducer of virulence gene expression. Striking bifurcation of the population occurred during entry into stationary phase: one subpopulation continued to express tcpA, whereas the expression declined in the other subpopulation. ctxA, encoding the A subunit of CT, and toxT, encoding the proximal master regulator of virulence gene expression also exhibited the bifurcation phenotype. The bifurcation phenotype was found to be reversible, epigenetic and to persist after removal of bicarbonate, features consistent with bistable switches. The bistable switch requires the positive-feedback circuit controlling ToxT expression and formation of the CRP-cAMP complex during entry into stationary phase. Key features of this bistable switch also were demonstrated in vivo, where striking heterogeneity in tcpA expression was observed in luminal fluid in later stages of the infection. When this fluid was diluted into artificial seawater, bacterial aggregates continued to express tcpA for prolonged periods of time. The bistable control of virulence gene expression points to a mechanism that could generate a subpopulation of V. cholerae that continues to produce TCP and CT in the rice water stools of cholera patients

Approach for Structurally Clearing an Adaptive Compliant Trailing Edge Flap for Flight

The Adaptive Compliant Trailing Edge (ACTE) flap was flown on the National Aeronautics and Space Administration (NASA) Gulfstream GIII testbed at the NASA Armstrong Flight Research Center. This smoothly curving flap replaced the existing Fowler flaps creating a seamless control surface. This compliant structure, developed by FlexSys Inc. in partnership with the Air Force Research Laboratory, supported NASA objectives for airframe structural noise reduction, aerodynamic efficiency, and wing weight reduction through gust load alleviation. A thorough structures airworthiness approach was developed to move this project safely to flight. A combination of industry and NASA standard practice require various structural analyses, ground testing, and health monitoring techniques for showing an airworthy structure. This paper provides an overview of compliant structures design, the structural ground testing leading up to flight, and the flight envelope expansion and monitoring strategy. Flight data will be presented, and lessons learned along the way will be highlighted

NSTAR Ion Thruster and Breadboard Power Processor Functional Integration Test Results

A 2.3 kW Breadboard Power Processing Unit (BBPPU) was developed as part of the NASA Solar Electric Propulsion Technology Application Readiness (NSTAR) Program. The NSTAR program will deliver an electric propulsion system based on a 30 cm xenon ion thruster to the New Millennium (NM) program for use as the primary propulsion system for the initial NM flight. The final development test for the BBPPU, the Functional Integration Test, was carried out to demonstrate all aspects of BBPPU operation with an Engineering Model Thruster. Test objectives included: (1) demonstration and validation of automated thruster start procedures, (2) demonstration of stable closed loop control of the thruster beam current, (3) successful response and recovery to thruster faults, and (4) successful safing of the system during simulated spacecraft faults. These objectives were met over the specified 80-120 VDC input voltage range and 0.5-2.3 output power capability of the BBPPU. Two minor anomalies were noted in discharge and neutralizer keeper current. These anomalies did not affect the stability of the system and were successfully corrected

Inherited crustal deformation along the East Gondwana margin revealed by seismic anisotropy tomography

Acknowledgments We thank Mallory Young for providing phase velocity measurements in mainland Australia and Tasmania. Robert Musgrave is thanked for making available his tilt-filtered magnetic intensity map. In the short term, data may be made available by contacting the authors (S.P. or N.R.). A new database of passive seismic data recorded in Australia is planned as part of a national geophysics data facility for easy access download. Details on the status of this database may be obtained from the authors (S.P., N.R., or A.M.R.). There are no restrictions on access for noncommercial use. Commercial users should seek written permission from the authors (S.P. or N.R.). Ross Cayley publishes with the permission of the Director of the Geological Survey of Victoria.Peer reviewedPublisher PD

Development Status of the NSTAR Ion Propulsion System Power Processor

A 0.5-2.3 kW xenon ion propulsion system is presently being developed under the NASA Solar Electric Propulsion Technology Application Readiness (NSTAR) program. This propulsion system includes a 30 cm diameter xenon ion thruster, a Digital Control Interface Unit, a xenon feed system, and a power processing unit (PPU). The PPU consists of the power supply assemblies which operate the thruster neutralizer, main discharge chamber, and ion optics. Also included are recycle logic and a digital microcontroller. The neutralizer and discharge power supplies employ a dual use configuration which combines the functions of two power supplies into one, significantly simplifying the PPU. Further simplification was realized by implementing a single thruster control loop which regulates the beam current via the discharge current. Continuous throttling is possible over a 0.5-2.3 kW output power range. All three power supplies have been fabricated and tested with resistive loads, and have been combined into a single breadboard unit with the recycle logic and microcontroller. All line and load regulation test results show the power supplies to be within the NSTAR flight PPU specified power output of 1.98 kW. The overall efficiency of the PPU, calculated as the combined efficiencies of the power supplies and controller, at 2.3 kW delivered to resistive loads was 0.90. The component was 6.16 kg. Integration testing of the neutralizer and discharge power supplies with a functional model thruster revealed no issues with discharge ignition or steady state operation

FY2010 Annual Report for the Actinide Isomer Detection Project

This project seeks to identify a new signature for actinide element detection in active interrogation. This technique works by exciting and identifying long-lived nuclear excited states (isomers) in the actinide isotopes and/or primary fission products. Observation of isomers in the fission products will provide a signature for fissile material. For the actinide isomers, the decay time and energy of the isomeric state is unique to a particular isotope, providing an unambiguous signature for Special Nuclear Materials (SNM). Future work will include a follow-up measurement scheduled for December 2010 at LBNL. Lessons learned from the July 2010 measurements will be incorporated into these new measurements. Analysis of both the July and December experiments will be completed in a few months. A research paper to be submitted to a peer-reviewed journal will be drafted if the conclusions from the measurements warrant publication

Seasonal prediction of bottom temperature on the Northeast U.S. Continental Shelf

© The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Chen, Z., Kwon, Y.-O., Chen, K., Fratantoni, P., Gawarkiewicz, G., Joyce, T. M., Miller, T. J., Nye, J. A., Saba, V. S., & Stock, B. C. Seasonal prediction of bottom temperature on the Northeast U.S. Continental Shelf. Journal of Geophysical Research: Oceans, 126(5), (2021): e2021JC017187, https://doi.org/10.1029/2021JC017187.The Northeast U.S. shelf (NES) is an oceanographically dynamic marine ecosystem and supports some of the most valuable demersal fisheries in the world. A reliable prediction of NES environmental variables, particularly ocean bottom temperature, could lead to a significant improvement in demersal fisheries management. However, the current generation of climate model-based seasonal-to-interannual predictions exhibits limited prediction skill in this continental shelf environment. Here, we have developed a hierarchy of statistical seasonal predictions for NES bottom temperatures using an eddy-resolving ocean reanalysis data set. A simple, damped local persistence prediction model produces significant skill for lead times up to ∼5 months in the Mid-Atlantic Bight and up to ∼10 months in the Gulf of Maine, although the prediction skill varies notably by season. Considering temperature from a nearby or upstream (i.e., more poleward) region as an additional predictor generally improves prediction skill, presumably as a result of advective processes. Large-scale atmospheric and oceanic indices, such as Gulf Stream path indices (GSIs) and the North Atlantic Oscillation Index, are also tested as predictors for NES bottom temperatures. Only the GSI constructed from temperature observed at 200 m depth significantly improves the prediction skill relative to local persistence. However, the prediction skill from this GSI is not larger than that gained using models incorporating nearby or upstream shelf/slope temperatures. Based on these results, a simplified statistical model has been developed, which can be tailored to fisheries management for the NES.This work was supported by NOAA's Climate Program Office's Modeling, Analysis, Predictions, and Projections (MAPP) Program (NA17OAR4310111, NA19OAR4320074), and Climate Program Office's Climate Variability and Predictability (CVP) Program (NA20OAR4310482). We acknowledge our participation in MAPP's Marine Prediction Task Force

Shared Bacterial and Viral Respiratory Agents in Bighorn Sheep (Ovis canadensis), Domestic Sheep (Ovis aries), and Goats (Capra hircus) in Montana

Transmission of infectious agents from livestock reservoirs has been hypothesized to cause respiratory disease outbreaks in bighorn sheep (Ovis canadensis), and land management policies intended to limit this transmission have proven controversial. This cross-sectional study compares the infectious agents present in multiple populations of bighorn sheep near to and distant from their interface with domestic sheep (O. aries) and domestic goat (Capra hircus) and provides critical baseline information needed for interpretations of cross-species transmission risks. Bighorn sheep and livestock shared exposure to Pasteurellaceae, viral, and endoparasite agents. In contrast, although the impact is uncertain, Mycoplasma sp. was isolated from livestock but not bighorn sheep. These results may be the result of historic cross-species transmission of agents that has resulted in a mosaic of endemic and exotic agents. Future work using longitudinal and multiple population comparisons is needed to rigorously establish the risk of outbreaks from cross-species transmission of infectious agents

Widespread movement of invasive cattle fever ticks (Rhipicephalus microplus) in southern Texas leads to shared local infestations on cattle and deer

Background: Rhipicephalus (Boophilus) microplus is a highly-invasive tick that transmits the cattle parasites (Babesia bovis and B. bigemina) that cause cattle fever. R. microplus and Babesia are endemic in Mexico and ticks persist in the United States inside a narrow tick eradication quarantine area (TEQA) along the Rio Grande. This containment area is threatened by unregulated movements of illegal cattle and wildlife like white-tailed deer (WTD; Odocoileus virginianus). Methods: Using 11 microsatellite loci we genotyped 1,247 R. microplus from 63 Texas collections, including outbreak infestations from outside the TEQA. We used population genetic analyses to test hypotheses about ecological persistence, tick movement, and impacts of the eradication program in southern Texas. We tested acaricide resistance with larval packet tests (LPTs) on 47 collections. Results: LPTs revealed acaricide resistance in 15/47 collections (32%); 11 were outside the TEQA and three were resistant to multiple acaricides. Some collections highly resistant to permethrin were found on cattle and WTD. Analysis of genetic differentiation over time at seven properties revealed local gene pools with very low levels of differentiation (F-ST 0.00-0.05), indicating persistence over timespans of up to 29 months. However, in one neighborhood differentiation varied greatly over a 12-month period (F-ST 0.03-0.13), suggesting recurring immigration from distinct sources as another persistence mechanism. Ticks collected from cattle and WTD at the same location are not differentiated (F-ST = 0), implicating ticks from WTD as a source of ticks on cattle (and vice versa) and emphasizing the importance of WTD to tick control strategies. We identified four major genetic groups (K = 4) using Bayesian population assignment, suggesting multiple introductions to Texas. Conclusions: Two dispersal mechanisms give rise to new tick infestations: 1) frequent short-distance dispersal from the TEQA; and 2) rare long-distance, human-mediated dispersal from populations outside our study area, probably Mexico. The threat of cattle fever tick transport into Texas is increased by acaricide resistance and the ability of R. microplus to utilize WTD as an alternate host. Population genetic analyses may provide a powerful tool for tracking invasions in other parts of the world where these ticks are established