PrPCWD lymphoid cell targets in early and advanced chronic wasting disease of mule deer

Up to 15% of free-ranging mule deer in northeastern Colorado and southeastern Wyoming, USA, are afflicted with a prion disease, or transmissible spongiform encephalopathy (TSE), known as chronic wasting disease (CWD). CWD is similar to a subset of TSEs including scrapie and variant Creutzfeldt¿Jakob disease in which the abnormal prion protein isoform, PrPCWD, accumulates in lymphoid tissue. Experimental scrapie studies have indicated that this early lymphoid phase is an important constituent of prion replication interposed between mucosal entry and central nervous system accumulation. To identify the lymphoid target cells associated with PrPCWD, we used triple-label immunofluorescence and high-resolution confocal microscopy on tonsils from naturally infected deer in advanced disease. We detected PrPCWD primarily extracellularly in association with follicular dendritic and B cell membranes as determined by frequent co-localization with antibodies against membrane bound immunoglobulin and CD21. There was minimal co-localization with cytoplasmic labels for follicular dendritic cells (FDC). This finding could indicate FDC capture of PrPCWD, potentially in association with immunoglobulin or complement, or PrPC conversion on FDC. In addition, scattered tingible body macrophages in the germinal centre contained coarse intracytoplasmic aggregates of PrPCWD, reflecting either phagocytosis of PrPCWD on FDC processes, apoptotic FDC or B cells, or actual PrPCWD replication within tingible body macrophages. To compare lymphoid cell targets in early and advanced disease, we also examined: (i) PrPCWD distribution in lymphoid cells of fawns within 3 months of oral CWD exposure and (ii) tonsil biopsies from preclinical deer with naturally acquired CWD. These studies revealed that the early lymphoid cellular distribution of PrPCWD was similar to that in advanced disease, i.e. in a pattern suggesting FDC association. We conclude that in deer, PrPCWD accumulates primarily extracellularly and associated with FDCs and possibly B cells ¿ a finding which raises questions as to the cells responsible for pathological prion productio

Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications

Hydroxyapatite (HA) has been widely used as a medical ceramic due to its good biocompatibility and osteoconductivity. Recently there has been interest regarding the use of bioinspired nanoscale hydroxyapatite (nHA). However, biological apatite is known to be calcium-deficient and carbonate-substituted with a nanoscale platelet-like morphology. Bioinspired nHA has the potential to stimulate optimal bone tissue regeneration due to its similarity to bone and tooth enamel mineral. Many of the methods currently used to fabricate nHA both in the laboratory and commercially, involve lengthy processes and complex equipment. Therefore, the aim of this study was to develop a rapid and reliable method to prepare high quality bioinspired nHA. The rapid mixing method developed was based upon an acid-base reaction involving calcium hydroxide and phosphoric acid. Briefly, a phosphoric acid solution was poured into a calcium hydroxide solution followed by stirring, washing and drying stages. Part of the batch was sintered at 1,000 °C for 2 h in order to investigate the products' high temperature stability. X-ray diffraction analysis showed the successful formation of HA, which showed thermal decomposition to β-tricalcium phosphate after high temperature processing, which is typical for calcium-deficient HA. Fourier transform infrared spectroscopy showed the presence of carbonate groups in the precipitated product. The nHA particles had a low aspect ratio with approximate dimensions of 50 x 30 nm, close to the dimensions of biological apatite. The material was also calcium deficient with a Ca:P molar ratio of 1.63, which like biological apatite is lower than the stoichiometric HA ratio of 1.67. This new method is therefore a reliable and far more convenient process for the manufacture of bioinspired nHA, overcoming the need for lengthy titrations and complex equipment. The resulting bioinspired HA product is suitable for use in a wide variety of medical and consumer health applications

Spectral signatures of photosynthesis I: Review of Earth organisms

Why do plants reflect in the green and have a 'red edge' in the red, and should extrasolar photosynthesis be the same? We provide: 1) a brief review of how photosynthesis works; 2) an overview of the diversity of photosynthetic organisms, their light harvesting systems, and environmental ranges; 3) a synthesis of photosynthetic surface spectral signatures; 4) evolutionary rationales for photosynthetic surface reflectance spectra with regard to utilization of photon energy and the planetary light environment. Given the surface incident photon flux density spectrum and resonance transfer in light harvesting, we propose some rules with regard to where photosynthetic pigments will peak in absorbance: a) the wavelength of peak incident photon flux; b) the longest available wavelength for core antenna or reaction center pigments; and c) the shortest wavelengths within an atmospheric window for accessory pigments. That plants absorb less green light may not be an inefficient legacy of evolutionary history, but may actually satisfy the above criteria.Comment: 69 pages, 7 figures, forthcoming in Astrobiology March 200

The Opportunistic Transmission of Wireless Worms between Mobile Devices

The ubiquity of portable wireless-enabled computing and communications devices has stimulated the emergence of malicious codes (wireless worms) that are capable of spreading between spatially proximal devices. The potential exists for worms to be opportunistically transmitted between devices as they move around, so human mobility patterns will have an impact on epidemic spread. The scenario we address in this paper is proximity attacks from fleetingly in-contact wireless devices with short-range communication range, such as Bluetooth-enabled smart phones. An individual-based model of mobile devices is introduced and the effect of population characteristics and device behaviour on the outbreak dynamics is investigated. We show through extensive simulations that in the above scenario the resulting mass-action epidemic models remain applicable provided the contact rate is derived consistently from the underlying mobility model. The model gives useful analytical expressions against which more refined simulations of worm spread can be developed and tested.Comment: Submitted for publicatio

Evaluating a workspace's usefulness for image retrieval

Image searching is a creative process. We have proposed a novel image retrieval system that supports creative search sessions by allowing the user to organise their search results on a workspace. The workspace’s usefulness is evaluated in a task-oriented and user-centred comparative experiment, involving design professionals and several types of realistic search tasks. In particular, we focus on its effect on task conceptualisation and query formulation. A traditional relevance feedback system serves as a baseline. The results of this study show that the workspace is more useful in terms of both of the above aspects and that the proposed approach leads to a more effective and enjoyable search experience. This paper also highlights the influence of tasks on the users’ search and organisation strategy

The role of plant species and soil condition in the structural development of the rhizosphere

© 2019 The Authors Plant, Cell & Environment Published by John Wiley & Sons Ltd Roots naturally exert axial and radial pressures during growth, which alter the structural arrangement of soil at the root–soil interface. However, empirical models suggest soil densification, which can have negative impacts on water and nutrient uptake, occurs at the immediate root surface with decreasing distance from the root. Here, we spatially map structural gradients in the soil surrounding roots using non-invasive imaging, to ascertain the role of root growth in early stage formation of soil structure. X-ray computed tomography provided a means not only to visualize a root system in situ and in 3-D but also to assess the precise root-induced alterations to soil structure close to, and at selected distances away from the root–soil interface. We spatially quantified the changes in soil structure generated by three common but contrasting plant species (pea, tomato, and wheat) under different soil texture and compaction treatments. Across the three plant types, significant increases in porosity at the immediate root surface were found in both clay loam and loamy sand soils and not soil densification, the currently assumed norm. Densification of the soil was recorded, at some distance away from the root, dependent on soil texture and plant type. There was a significant soil texture×bulk density×plant species interaction for the root convex hull, a measure of the extent to which root systems explore the soil, which suggested pea and wheat grew better in the clay soil when at a high bulk density, compared with tomato, which preferred lower bulk density soils. These results, only revealed by high resolution non-destructive imagery, show that although the root penetration mechanisms can lead to soil densification (which could have a negative impact on growth), the immediate root–soil interface is actually a zone of high porosity, which is very important for several key rhizosphere processes occurring at this scale including water and nutrient uptake and gaseous diffusion

Effects of feeding low levels of crude glycerin with or without other by-products on performance and carcass characteristics of feedlot heifers

Expansion of the renewable fuels industries has increased availability of by-products that are well suited for use as cattle feed. Glycerin is among the principal by-products of biodiesel production, comprising approximately 10% (by weight) of the soybean oil that is used to manufacture soy-based diesel fuel. Our previous research evaluated effects of including between 0% and 16% glycerin in flaked-corn finishing diets and revealed that optimal growth performance was achieved with 2% glycerin addition. Our laboratory experiments have suggested that even lower levels of glycerin may be effective at stimulating digestion. Therefore, the objective of this study was to evaluate effects of low levels of glycerin in the diet on performance and carcass characteristics of finishing cattle. Furthermore, because distillers grains and other by-products are increasingly common in feedlot rations, we opted to evaluate glycerin in corn-based finishing diets as well as in diets that consisted of a combination of corn grain, distillers grains, and soybean hulls

Modern pollution signals in sediments from Windermere, NW England, determined by micro-XRF and lead isotope analysis

High resolution geochemical (Itrax micro-XRF and wavelength dispersive XRF) data, radiochronology (210Pb and 137Cs analyses) and ultra-high precision double-spike lead isotope measurements from lacustrine sediment cores are used in combination with historical research of former mining landscapes to investigate modern pollution signals in sediments from Windermere, the largest lake in the English Lake District. The sediment record suggests that while most element concentrations have been stable, there has been a significant increase since the 1930s in lead, zinc and copper concentrations. Double-spike lead isotope measurements reveal a mixture of natural lead, and three major contributory sources of anthropogenic (industrial) lead, comprising gasoline lead, coal combustion lead (from coal-fired steam ships) and lead derived from Carboniferous Pb–Zn mineralisation (mining activities). A number of up-system sediment traps have limited the amount of mining related heavy metals entering Windermere, and as a result, periods of metal workings do not correlate with peaks in heavy metals. Increases could also be due to flood-induced metal inwash or weathering of bedrock in the catchment. Application of these non-destructive and high precision analytical techniques provides new insights into the pollutant depositional history of Windermere