3,673 research outputs found
Acidosis Is a Key Regulator of Osteoblast Ecto-Nucleotidase Pyrophosphatase/Phosphodiesterase 1 (NPP1) Expression and Activity
Previous work has shown that acidosis prevents bone nodule formation by osteoblasts in vitro by inhibiting mineralisation of the collagenous matrix. The ratio of phosphate (Pi) to pyrophosphate (PPi) in the bone microenvironment is a fundamental regulator of bone mineralisation. Both Pi and PPi, a potent inhibitor of mineralisation, are generated from extracellular nucleotides by the actions of ectoânucleotidases. This study investigated the expression and activity of ectoânucleotidases by osteoblasts under normal and acid conditions. We found that osteoblasts express mRNA for a number of ectoânucleotidases including NTPdase 1â6 (ectoânucleoside triphosphate diphosphohydrolase) and NPP1â3 (ectoânucleotide pyrophosphatase/phosphodiesterase). The rank order of mRNA expression in differentiating rat osteoblasts (day 7) was Enpp1â>âNTPdase 4â>âNTPdase 6â>âNTPdase 5â>âalkaline phosphataseâ>âectoâ5ânucleotidaseâ>âEnpp3â>âNTPdase 1â>âNTPdase 3â>âEnpp2â>âNTPdase 2. Acidosis (pH 6.9) upregulated NPP1 mRNA (2.8âfold) and protein expression at all stages of osteoblast differentiation compared to physiological pH (pH 7.4); expression of other ectoânucleotidases was unaffected. Furthermore, total NPP activity was increased up to 53% in osteoblasts cultured in acid conditions (Pâ<â0.001). Release of ATP, one of the key substrates for NPP1, from osteoblasts, was unaffected by acidosis. Further studies showed that mineralised bone formation by osteoblasts cultured from NPP1 knockout mice was increased compared with wildtypes (2.5âfold, Pâ<â0.001) and was partially resistant to the inhibitory effect of acidosis. These results indicate that increased NPP1 expression and activity might contribute to the decreased mineralisation observed when osteoblasts are exposed to acid conditions
Nulling Data Reduction and On-sky Performance of the Large Binocular Telescope Interferometer
The Large Binocular Telescope Interferometer (LBTI) is a versatile instrument designed for high angular resolution and high-contrast infrared imaging (1.5â13 Îźm). In this paper, we focus on the mid-infrared (8â13 Îźm) nulling mode and present its theory of operation, data reduction, and on-sky performance as of the end of the commissioning phase in 2015 March. With an interferometric baseline of 14.4 m, the LBTI nuller is specifically tuned to resolve the habitable zone of nearby main-sequence stars, where warm exozodiacal dust emission peaks. Measuring the exozodi luminosity function of nearby main-sequence stars is a key milestone to prepare for future exo-Earth direct imaging instruments. Thanks to recent progress in wavefront control and phase stabilization, as well as in data reduction techniques, the LBTI demonstrated in 2015 February a calibrated null accuracy of 0.05% over a 3 hr long observing sequence on the bright nearby A3V star β Leo. This is equivalent to an exozodiacal disk density of 15â30 zodi for a Sun-like star located at 10 pc, depending on the adopted disk model. This result sets a new record for high-contrast mid-infrared interferometric imaging and opens a new window on the study of planetary systems
Context and collaborative problem solving (Cps): The development of observable signifiers to inform the design of cps learning analytics
Collaborative Problem Solving (CPS) is a key skill for the modern workplace. We do not, however, have widely accepted ways of assessing and monitoring CPS to inform educators and learners and enable the provision of effective support. This paper reports the findings of an empirical study involving 15 school students aged 14-15 years taking part in a 2-day Hack Event. The analysis identifies the observable signifiers of CPS and offers a step towards the design of automated data capture protocols and CPS learning analytics
Imaging the asymmetric dust shell around CI Cam with long baseline optical interferometry
We present the first high angular resolution observation of the B[e]
star/X-ray transient object CI Cam, performed with the two-telescope Infrared
Optical Telescope Array (IOTA), its upgraded three-telescope version (IOTA3T)
and the Palomar Testbed Interferometer (PTI). Visibilities and closure phases
were obtained using the IONIC-3 integrated optics beam combiner. CI Cam was
observed in the near-infrared H and K spectral bands, wavelengths well suited
to measure the size and study the geometry of the hot dust surrounding CI Cam.
The analysis of the visibility data over an 8 year period from soon after the
1998 outburst to 2006 shows that the dust visibility has not changed over the
years. The visibility data shows that CI Cam is elongated which confirms the
disc-shape of the circumstellar environment and totally rules out the
hypothesis of a spherical dust shell. Closure phase measurements show direct
evidence of asymmetries in the circumstellar environment of CI Cam and we
conclude that the dust surrounding CI Cam lies in an inhomogeneous disc seen at
an angle. The near-infrared dust emission appears as an elliptical skewed
Gaussian ring with a major axis a = 7.58 +/- 0.24 mas, an axis ratio r = 0.39
+/- 0.03 and a position angle theta = 35 +/- 2 deg.Comment: 9 pages, 5 figures, accepted MNRA
First L-band Interferometric Observations of a Young Stellar Object: Probing the Circumstellar Environment of MWC 419
We present spatially-resolved K- and L-band spectra (at spectral resolution R
= 230 and R = 60, respectively) of MWC 419, a Herbig Ae/Be star. The data were
obtained simultaneously with a new configuration of the 85-m baseline Keck
Interferometer. Our observations are sensitive to the radial distribution of
temperature in the inner region of the disk of MWC 419. We fit the visibility
data with both simple geometric and more physical disk models. The geometric
models (uniform disk and Gaussian) show that the apparent size increases
linearly with wavelength in the 2-4 microns wavelength region, suggesting that
the disk is extended with a temperature gradient. A model having a power-law
temperature gradient with radius simultaneously fits our interferometric
measurements and the spectral energy distribution data from the literature. The
slope of the power-law is close to that expected from an optically thick disk.
Our spectrally dispersed interferometric measurements include the Br gamma
emission line. The measured disk size at and around Br gamma suggests that
emitting hydrogen gas is located inside (or within the inner regions) of the
dust disk.Comment: Accepted for publication in Ap
First Keck Nulling Observations of a Young Stellar Object: Probing the Circumstellar Environment of the Herbig Ae star MWC 325
We present the first N-band nulling plus K- and L-band V2 observations of a
young stellar object, MWC325, taken with the 85 m baseline Keck Interferometer.
The Keck nuller was designed for the study of faint dust signatures associated
with debris disks, but it also has a unique capability for studying the
temperature and density distribution of denser disks found around young stellar
objects. Interferometric observations of MWC 325 at K, L and N encompass a
factor of five in spectral range and thus, especially when spectrally dispersed
within each band, enable characterization of the structure of the inner disk
regions where planets form. Fitting our observations with geometric models such
as a uniform disk or a Gaussian disk show that the apparent size increases
monotonically with wavelength in the 2-12 um wavelength region, confirming the
widely held assumption based on radiative transfer models, now with spatially
resolved measurements over broad wavelength range, that disks are extended with
a temperature gradient. The effective size is a factor of about 1.3 and 2
larger in the L-band and N-band, respectively, compared to that in the K-band.
The existing interferometric measurements and the spectral energy distribution
can be reproduced by a flat disk or a weakly-shadowed nearly flat-disk model,
with only slight flaring in the outer regions of the disk, consisting of
representative "sub-micron" (0.1 um) and "micron" (2 um) grains of a 50:50
ratio of silicate and graphite. This is marked contrast with the disks
previously found in other Herbig Ae/Be stars suggesting a wide variety in the
disk properties among Herbig Ae/Be stars.Comment: Accepted for publication in the Ap
Keck Interferometer nuller instrument performance
The Keck Interferometer combines the two 10 m Keck telescopes as a long baseline interferometer. It is funded by NASA as a joint development among the Jet Propulsion Laboratory, the W. M. Keck Observatory, and the NASA Exoplanet Science Institute. In February 2008, the 10 um nulling mode began a 32 night observing program with three key science teams to perform a survey of nearby stars for exozodiacal dust. This program has recently concluded, and has been followed by nuller observing on a variety of science topics through the standard proposal process. We provide a review and update of the nuller implementation, and describe the data reduction process, including the calibration approach. We then review the technical performance of the instrument based on the full key science data set, including sensitivity and systematic errors. We also provide some summary data on atmospheric effects applicable to the cophasing approach
Mid-infrared size survey of Young Stellar Objects: Description of Keck segment-tilting experiment and basic results
The mid-infrared properties of pre-planetary disks are sensitive to the
temperature and flaring profiles of disks for the regions where planet
formation is expected to occur. In order to constrain theories of planet
formation, we have carried out a mid-infrared (wavelength 10.7 microns) size
survey of young stellar objects using the segmented Keck telescope in a novel
configuration. We introduced a customized pattern of tilts to individual mirror
segments to allow efficient sparse-aperture interferometry, allowing full
aperture synthesis imaging with higher calibration precision than traditional
imaging. In contrast to previous surveys on smaller telescopes and with poorer
calibration precision, we find most objects in our sample are partially
resolved. Here we present the main observational results of our survey of 5
embedded massive protostars, 25 Herbig Ae/Be stars, 3 T Tauri stars, 1 FU Ori
system, and 5 emission-line objects of uncertain classification. The observed
mid-infrared sizes do not obey the size-luminosity relation found at
near-infrared wavelengths and a companion paper will provide further modelling
analysis of this sample. In addition, we report imaging results for a few of
the most resolved objects, including complex emission around embedded massive
protostars, the photoevaporating circumbinary disk around MWC 361A, and the
subarcsecond binaries T Tau, FU Ori and MWC 1080.Comment: Accepted by Astrophysical Journal. 38 pages. 9 figure
Combining brain-computer interfaces and assistive technologies: state-of-the-art and challenges
In recent years, new research has brought the field of EEG-based Brain-Computer Interfacing (BCI) out of its infancy and into a phase of relative maturity through many demonstrated prototypes such as brain-controlled wheelchairs, keyboards, and computer games. With this proof-of-concept phase in the past, the time is now ripe to focus on the development of practical BCI technologies that can be brought out of the lab and into real-world applications. In particular, we focus on the prospect of improving the lives of countless disabled individuals through a combination of BCI technology with existing assistive technologies (AT). In pursuit of more practical BCIs for use outside of the lab, in this paper, we identify four application areas where disabled individuals could greatly benefit from advancements in BCI technology, namely,âCommunication and Controlâ, âMotor Substitutionâ, âEntertainmentâ, and âMotor Recoveryâ. We review the current state of the art and possible future developments, while discussing the main research issues in these four areas. In particular, we expect the most progress in the development of technologies such as hybrid BCI architectures, user-machine adaptation algorithms, the exploitation of usersâ mental states for BCI reliability and confidence measures, the incorporation of principles in human-computer interaction (HCI) to improve BCI usability, and the development of novel BCI technology including better EEG devices
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