Halo ellipticity of GAMA galaxy groups from KiDS weak lensing

We constrain the average halo ellipticity of ~2 600 galaxy groups from the Galaxy And Mass Assembly (GAMA) survey, using the weak gravitational lensing signal measured from the overlapping Kilo Degree Survey (KiDS). To do so, we quantify the azimuthal dependence of the stacked lensing signal around seven different proxies for the orientation of the dark matter distribution, as it is a priori unknown which one traces the orientation best. On small scales, the major axis of the brightest group/cluster member (BCG) provides the best proxy, leading to a clear detection of an anisotropic signal. In order to relate that to a halo ellipticity, we have to adopt a model density profile. We derive new expressions for the quadrupole moments of the shear field given an elliptical model surface mass density profile. Modeling the signal with an elliptical Navarro-Frenk-White (NFW) profile on scales < 250 kpc, which roughly corresponds to half the virial radius, and assuming that the BCG is perfectly aligned with the dark matter, we find an average halo ellipticity of e_h=0.38 +/- 0.12. This agrees well with results from cold-dark-matter-only simulations, which typically report values of e_h ~ 0.3. On larger scales, the lensing signal around the BCGs does not trace the dark matter distribution well, and the distribution of group satellites provides a better proxy for the halo's orientation instead, leading to a 3--4 sigma detection of a non-zero halo ellipticity at scales between 250 kpc and 750 kpc. Our results suggest that the distribution of stars enclosed within a certain radius forms a good proxy for the orientation of the dark matter within that radius, which has also been observed in hydrodynamical simulations

Meta-analyses of genome-wide association studies for postpartum depression

Objective: Postpartum depression (PPD) is a common subtype of major depressive disorder (MDD) that is more heritable, yet is understudied in psychiatric genetics. The authors conducted meta-analyses of genome-wide association studies (GWASs) to investigate the genetic architecture of PPD. Method: Meta-analyses were conducted on 18 cohorts of European ancestry (17,339 PPD cases and 53,426 controls), one cohort of East Asian ancestry (975 cases and 3,780 controls), and one cohort of African ancestry (456 cases and 1,255 controls), totaling 18,770 PPD cases and 58,461 controls. Post-GWAS analyses included 1) single-nucleotide polymorphism (SNP)–based heritability (), 2) genetic correlations between PPD and other phenotypes, and 3) enrichment of the PPD GWAS findings in 27 human tissues and 265 cell types from the mouse central and peripheral nervous system. Results: No SNP achieved genome-wide significance in the European or the trans-ancestry meta-analyses. The of PPD was 0.14 (SE=0.02). Significant genetic correlations were estimated for PPD with MDD, bipolar disorder, anxiety disorders, posttraumatic stress disorder, insomnia, age at menarche, and polycystic ovary syndrome. Cell-type enrichment analyses implicate inhibitory neurons in the thalamus and cholinergic neurons within septal nuclei of the hypothalamus, a pattern that differs from MDD. Conclusions: While more samples are needed to reach genome-wide levels of significance, the results presented confirm PPD as a polygenic and heritable phenotype. There is also evidence that despite a high correlation with MDD, PPD may have unique genetic components. Cell enrichment results suggest GABAergic neurons, which converge on a common mechanism with the only medication approved by the U.S. Food and Drug Administration for PPD (brexanolone)

Récepteurs stéroïdiens et mécanismes d’action des stéroïdes sexuels

Les récepteurs stéroïdiens définissent une large famille de protéines. Un second récepteur des œstrogènes a été récemment identifié. Cette découverte implique l’existence d’un nouveau mécanisme d’action des œstrogènes faisant intervenir la formation d’hétérodimères. Elle a également des conséquences importantes en pharmacologie. L’activation des récepteurs par leur ligand entraîne la modulation de la transcription de gènes spécifiques. Les protéines impliquées dans cet effet ont été récemment identifiées. Ce sont des coactivateurs, des corépresseurs et des cointégrateurs. Leur mécanisme d’action a été caractérisé. Il fait intervenir des modifications de l’acétylation des histones au voisinage du promoteur correspondant. Les récepteurs des stéroïdes sexuels sont localisés dans le noyau et font en permanence une navette entre le noyau et le cytoplasme. Des effets non génomiques des stéroïdes ont également été décrits

Anti-Human FSH Receptor Monoclonal Antibodies: Immunochemical and Immunocytochemical Characterization of the Receptor

International audienceThe extracellular domain of the human FSH receptor was expressed in Escherichia coli as a fusion protein with ubiquitin. It was tagged with a poly-His tract which was used for its purification. Immunization of mice allowed the preparation of high affinity antireceptor monoclonal antibodies. The latter fell into two categories:  some of them were inhibited hormone binding and adenylate cyclase activation whereas others were devoid of these properties. None of the antibodies had agonistic activity (i.e., stimulated adenylate cyclase). Immunoaffinity chromatography allowed us to purify the native receptor in a single step either from a permanently transfected L cell line (75% recovery) or from human ovaries (33% recovery). Immunoblotting of the receptor in human ovaries showed the presence of a major band of 87 kDa and of a minor band of 81 kDa. Endoglycosidase digestion and pulse−chase experiments showed the former to be the mature receptor and the latter the precursor containing mannose-rich carbohydrates. Thus, as in the case for the LH receptor, there was an accumulation (albeit to a lower degree) of the precursor in target cells. We did not detect variant forms of the protein corresponding to the alternative mRNA transcripts previously described. Additive binding to the receptor of several antibodies, but not of the same antibody, allowed us to establish a sandwich-type ELISA for the receptor (sensitivity ∼1 fmol) and to obtain evidence against the existence of previously described oligomeric forms of the protein. All monoclonal antibodies were able to label the receptor immunocytochemically in transfected cells, and two of them were also able to detect it at the markedly lower physiological concentrations, i.e., in human Sertoli and granulosa cells