Signatures of present and past melt distribution along fast and intermediate spreading centers

The work presented in this dissertation depicts past and present signatures of melt distribution at fast and intermediate spreading centers. The primary goal of the studies included in this thesis is to provide better understanding of melt distribution and variation in melt physical properties within and at the base of oceanic crust formed at these spreading centers. Furthermore, this work examines effects that melt presence might have on formation and structural characteristics of oceanic crust. To explore the above we use geophysical data collected during two expeditions conducted along the Juan de Fuca Ridge (intermediate) and the East Pacific Rise (fast). The major part of the thesis is based on the work conducted on high resolution reflection seismic data that investigate present day intracrustal melt distribution along the East Pacific Rise (EPR) axis extending between 8º20’ and 10º10’N. Here, the character of the melt reservoir is examined from different aspects and by using different seismic data analysis methods. By systematic analysis of the seismic reflection data, we show that the axial melt lens (AML) is segmented at different segment scales. Locations of the mapped disruptions in the AML correspond to previously identified tectonic discontinuities well expressed in the seafloor bathymetry. The above result corroborates genetic relationship between tectonic and magmatic segmentation. To examine melt distribution along the EPR, here for the first time we use amplitude variation with angle of incidence (AVA) crossplotting technique that was developed by oil and gas industry experts to look for presence of hydrocarbons. Further data examination for the first time for the mid-ocean ridges show presence of deeper lenses (lenses that are present below the AML). Presence of gaps in these sub-events and their collocation with what is believed to be the location of origin of the last documented eruption occurred in 2005-06, may shed light on the mechanisms behind the mid-ocean ridges volcanic processes. To explore variation in crustal structure and melt distribution at present day along the Juan de Fuca Ridge and relicts of past melt presence near ridge propagators wakes, a combination of gravity and multi-channel seismic data was used. Gravity modeling, constrained by seismic data, showed that robust topography (shallow axial depth and wide axial high) and thicker crust observed for the southern portion of this ridge system originate from enhanced melt supply at the base of the crust. In addition, prominent crustal thickening on the younger crust side of the inner propagators wakes (now on the ridge flanks) is brought into relationship with collocated frozen magma lenses imaged at the base of the crust. Spatial relationship of the two argues for their causal relationship at the time of the crustal formation on the axis. Our study suggests that these frozen lenses represent the record of once molten reservoir that most probably actively participated in the formation of the thicker crust

All-trans retinoic acid influences viability, migration and adhesion of U251 glioblastoma cells

Glioblastoma (GBM) is one of the most aggressive and deadly forms of cancer. Literature data reveals that all-trans retinoic acid (ATRA) has anticancer effects on different types of tumor cells. However, data about the effects of ATRA on glioblastoma cells are contradictory. In this study, we examined whether ATRA treatment affects features of human glioblastoma U251 cells. To that end, the cells were treated with different concentrations of ATRA. Results obtained by MTT and the crystal violet assays imply that ATRA affected the viability of U251 glioblastoma cells in a dose-and time-dependent manner. Fluorescence staining of microtubule cytoskeleton protein a-tubulin revealed that ATRA induced changes in cell morphology. Using semi-quantitative RT-PCR we found that the expression of SOX3 and GFAP genes, as markers of neural differentiation, was not changed upon ATRA treatment. Thus, the observed changes in cell morphology after ATRA treatment are not associated with neural differentiation of U251 glioblastoma cells. The scratch-wound healing assay revealed that ATRA changed the mode of U251 cell migration from collective to single cell motility. The cell-matrix adhesion assay demonstrated that the pharmacologically relevant concentration of ATRA lowered the cell-matrix adhesion capability of U251 cells. In conclusion, our results imply that further studies are needed before ATRA could be considered for the treatment of glioblastoma

Parametri kvaliteta plodova kod različitih genotipova paradajza u organskoj proizvodnji

The aim of this study was to examine the content of total soluble solids, organic acids and lycopene in fruits of different tomato genotypes grown in organic production. Four different genotypes (Volovsko heart Red cherry, Tigrella and Viva) were used during this experiment. The quality of tomato fruits was analyzed based on the measurement of total soluble solids, organic acids and lycopene in fresh weight of the fruit. The values obtained for the analyzed parameters of fruit quality were as follows: total soluble solids 4.98, 5.26, 5.28 and 4.70 (° Brix), organic acids 160.67, 110.34, 132.77 and 116 (citric acid µmol g-1 FW), lycopene, 8.22, 5.70, 7.61, 9.21 (mg kg-1 FW) in genotypes Volovsko srce, Red cherry, Tigrella and Viva. Oscillations in the obtained parameters, especially in the content of lycopene in different genotypes were probably the result of genetic resources, but also the influence of abiotic factors.Cilj ovog istraživanja je bio da se ispita sadržaj ukupnih rastvorljivih suvih materija, organskih kiselina i likopena u plodovima različitih genotipova paradajza gajenog u organskoj proizvodnji. Četiri različita genotipa (Volovsko srce, Red cherry, Tigrella i Viva) su korišćeni tokom ovog eksperimenta. Kvalitet plodova paradajza analiziran je na osnovu merenja ukupnih rastvorljivih suvih materija, organskih kiselina i likopena u svežoj masi plodova. Dobijene vrednosti za analizirane parametre kvaliteta plodova su iznosile: ukupne rastvorljive materije 4.98, 5.26, 5.28 i 4.70 (oBrix), organske kiseline 160.67, 110.34, 116 i 132.77 (citric acid µmol g-1 FW), likopen 8.22, 5.70, 7.61, 9.21 (mg kg-1 FW) kod analiziranih genotipova Volovsko srce, Red cherry, Tigrella i Viva. Variranje u dobijenim parametrima, a naročito u sadržaju likopena kod različitih genotipova su bile posledica genetskog potencijala, ali i uticaja abiotičkih faktora

A multi-sill magma plumbing system beneath the axis of the East Pacific Rise

Author Posting. © The Author(s), 2014. This is the author's version of the work. It is posted here by permission of Nature Publishing Group for personal use, not for redistribution. The definitive version was published in Nature Geoscience 7 (2014): 825-829, doi:10.1038/ngeo2272.The mid-crust axial magma lens detected at fast and intermediate spreading mid-ocean ridges is believed to be the primary magma reservoir for formation of upper oceanic crust. However, the mechanism behind formation of the lower crust is a subject of ongoing debate. The sheeted sill model proposed from observations of ophiloites requires the presence of multiple lenses/sills throughout lower crust but only a single lens is imaged directly beneath the innermost axial zone in prior seismic studies . Here, high-fidelity seismic data from the East Pacific Rise reveal series of reflections below the axial magma lens that we interpret as mid-lower crustal lenses. These deeper lenses are present between 9°20-57′N at variable two-way-travel-times, up to 4.6 s (~1.5 km beneath the axial magma lens), providing direct support for the sheeted sill model. From local changes in the amplitude and geometry of the events beneath a zone of recent volcanic eruption, we infer that melt drained from a lower lens contributed to the replenishment of the axial magma lens above and, perhaps, the eruption. The new data indicate that a multi-level sill complex is present beneath the East Pacific Rise that likely contributes to the formation of both the upper and lower crust.This research was supported by NSF awards OCE0327872 to J. C. M., S. M. C., OCE- 0327885 to J. P. C., and OCE0624401 to M. R. N.2015-04-1

The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1

The altered expression of the SOX2 transcription factor is associated with oncogenic or tumor suppressor functions in human cancers. This factor regulates the migration and invasion of different cancer cells. In this study we investigated the effect of constitutive SOX2 overexpression on the migration and adhesion capacity of embryonal teratocarcinoma NT2/D1 cells derived from a metastasis of a human testicular germ cell tumor. We detected that increased SOX2 expression changed the speed, mode and path of cell migration, but not the adhesion ability of NT2/D1 cells. Additionally, we demonstrated that SOX2 overexpression increased the expression of the tumor suppressor protein p53 and the HDM2 oncogene. Our results contribute to the better understanding of the effect of SOX2 on the behavior of tumor cells originating from a human testicular germ cell tumor. Considering that NT2/D1 cells resemble cancer stem cells in many features, our results could contribute to the elucidation of the role of SOX2 in cancer stem cells behavior and the process of metastasis

Distribution of melt along the East Pacific Rise from 9°30′ to 10°N from an amplitude variation with angle of incidence (AVA) technique

Author Posting. © The Author(s), 2015. This article is posted here by permission of The Royal Astronomical Society for personal use, not for redistribution. The definitive version was published in Geophysical Journal International 203 (2015): 1-21, doi:10.1093/gji/ggv251.We examine along-axis variations in melt content of the axial magma lens (AML) beneath the fast-spreading East Pacific Rise (EPR) using an amplitude variation with angle of incidence (AVA) crossplotting method applied to multichannel seismic data acquired in 2008. The AVA crossplotting method, which has been developed for and, so far, applied for hydrocarbon prospection in sediments, is for the first time applied to a hardrock environment. We focus our analysis on 2-D data collected along the EPR axis from 9°29.8′N to 9°58.4′N, a region which encompasses the sites of two well-documented submarine volcanic eruptions (1991–1992 and 2005–2006). AVA crossplotting is performed for a ∼53 km length of the EPR spanning nine individual AML segments (ranging in length from ∼3.2 to 8.5 km) previously identified from the geometry of the AML and disruptions in continuity. Our detailed analyses conducted at 62.5 m interval show that within most of the analysed segments melt content varies at spatial scales much smaller (a few hundred of metres) than the length of the fine-scale AML segments, suggesting high heterogeneity in melt concentration. At the time of our survey, about 2 yr after the eruption, our results indicate that the three AML segments that directly underlie the 2005–2006 lava flow are on average mostly molten. However, detailed analysis at finer-scale intervals for these three segments reveals AML pockets (from >62.5 to 812.5 m long) with a low melt fraction. The longest such mushy section is centred beneath the main eruption site at ∼9°50.4′N, possibly reflecting a region of primary melt drainage during the 2005–2006 event. The complex geometry of fluid flow pathways within the crust above the AML and the different response times of fluid flow and venting to eruption and magma reservoir replenishment may contribute to the poor spatial correlation between incidence of hydrothermal vents and presence of highly molten AML. The presented results are an important step forward in our ability to resolve small-scale characteristics of the AML and recommend the AVA crossplotting as a tool for examining mid-ocean ridge magma-systems elsewhere.This research was supported by NSF awards OCE0327872 to J.C.M. and S.M.C., OCE-0327885 to J.P.C., and OCE0624401 to M.R.N

Constraints on melt content of off-axis magma lenses at the East Pacific Rise from analysis of 3-D seismic amplitude variation with angle of incidence

Author Posting. © American Geophysical Union, 2017. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Solid Earth 122 (2017): 4123–4142, doi:10.1002/2016JB013785.We use 3-D multichannel seismic data to form partial angle P wave stacks and apply amplitude variation with angle (AVA) crossplotting to assess melt content and melt distribution within two large midcrustal off-axis magma lenses (OAMLs) found along the East Pacific Rise from 9°37.5′N to 9°57′N. The signal envelope of the partial angle stacks suggests that both OAMLs are partially molten with higher average melt content and more uniform melt distribution in the southern OAML than in the northern OAML. For AVA crossplotting, the OAMLs are subdivided into seven ~1 km2 analysis windows. The AVA crossplotting results indicate that the OAMLs contain a smaller amount of melt than the axial magma lens (AML). For both OAMLs, a higher melt fraction is detected within analysis windows located close to the ridge axis than within the most distant windows. The highest average melt concentration is interpreted for the central sections of the OAMLs. The overall low OAML melt content could be indicative of melt lost due to recent off-axis eruptions, drainage to the AML, or limited mantle melt supply. Based on the results of this and earlier bathymetric, morphological, geochemical, and geophysical investigations, we propose that the melt-poor OAML state is largely the result of limited melt supply from the underlying mantle source reservoir with smaller contribution attributed to melt leakage to the AML. We hypothesize that the investigated OAMLs have a longer period of melt replenishment, lower eruption recurrence rates, and lower eruption volumes than the AML, though some could be single intrusion events.National Science Foundation; CFI; CRC2017-12-2

Stacked magma lenses beneath mid-ocean ridges: insights from new seismic observations and synthesis with prior geophysical and geologic findings

Author Posting. © American Geophysical Union, 2021. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Solid Earth 126(4), (2021): e2020JB021434, https://doi.org/10.1029/2020JB021434.Recent multi-channel seismic studies of fast spreading and hot-spot influenced mid-ocean ridges reveal magma bodies located beneath the mid-crustal Axial Magma Lens (AML), embedded within the underlying crustal mush zone. We here present new seismic images from the Juan de Fuca Ridge that show reflections interpreted to be from vertically stacked magma lenses in a number of locations beneath this intermediate-spreading ridge. The brightest reflections are beneath Northern Symmetric segment, from ∼46°42′-52′N and Split Seamount, where a small magma body at local Moho depths is also detected, inferred to be a source reservoir for the stacked magma lenses in the crust above. The imaged magma bodies are sub-horizontal, extend continuously for along-axis lengths of ∼1–8 km, with the shallowest located at depths of ∼100–1,200 m below the AML, and are similar to sub-AML bodies found at the East Pacific Rise. At both ridges, stacked sill-like lenses are detected beneath only a small fraction of the ridge length examined and are inferred to mark local sites of higher melt flux and active replenishment from depth. The imaged magma lenses are focused in the upper part of the lower crust, which coincides with the most melt rich part of the crystal mush zone detected in other geophysical studies and where sub-vertical fabrics are observed in geologic exposures of oceanic crust. We infer that the multi-level magma accumulations are ephemeral and may result from porous flow and mush compaction, and that they can be tapped and drained during dike intrusion and eruption events.This research was supported by NSF OCE 0002488 and 0648303 (LDEO), 0002551 (WHOI), 1658199 and 1357076 (UTIG). S. M. Carbotte was partially supported by Columbia University and J. P. Canales by the Independent Research & Development Program at WHOI

IMPACT OF IRRIGATION REGIME AND APPLICATION OF KAOLIN ON THE STOMATAL CONDUCTANCE AND LEAF WATER POTENTIAL OF PEPPER AND TOMATO

The effect of different irrigation regimes and the application of kaolin on stomatal conductance and leaf water potential of pepper (Capsicum annuum L.) and tomato (Lycopersicon esculentum Mill.), is discussed in the paper.The experiment was set up by a completely randomized block system with three replications. The peppers were observed in three, and tomatoes in two treatments of irrigation regimes. The kaolin treatments of both crops were: a) control without kaolin (C) and 5% kaolin suspension (K).The results of these studies show that the application of deficit irrigation treatments and kaolin treatment have no statistically significant effect on stomatal conductance and leaf water potential of pepper and tomato plants

Seismic structure of the St. Paul Fracture Zone and Late Cretaceous to Mid Eocene oceanic crust in the equatorial Atlantic Ocean near 18°W

Plate tectonics characterize transform faults as conservative plate boundaries where the lithosphere is neither created nor destroyed. In the Atlantic, both transform faults and their inactive traces, fracture zones, are interpreted to be structurally heterogeneous, representing thin, intensely fractured, and hydrothermally altered basaltic crust overlying serpentinized mantle. This view, however, has recently been challenged. Instead, transform zone crust might be magmatically augmented at ridge-transform intersections before becoming a fracture zone. Here, we present constraints on the structure of oceanic crust from seismic refraction and wide-angle data obtained along and across the St. Paul fracture zone near 18°W in the equatorial Atlantic Ocean. Most notably, both crust along the fracture zone and away from it shows an almost uniform thickness of 5-6 km, closely resembling normal oceanic crust. Further, a well-defined upper mantle refraction branch supports a normal mantle velocity of 8 km/s along the fracture zone valley. Therefore, the St. Paul fracture zone reflects magmatically accreted crust instead of the anomalous hydrated lithosphere. Little variation in crustal thickness and velocity structure along a 200 km long section across the fracture zone suggests that distance to a transform fault had negligible impact on crustal accretion. Alternatively, it could also indicate that a second phase of magmatic accretion at the proximal ridge-transform intersection overprinted features of starved magma supply occurring along transform faults. Key Points: - Seismic structure along the St. Paul fracture zone reflects magmatically accreted oceanic crust - Oceanic crust across St. Paul shows only small thickness variations, lacking evidence for regional crustal thinning near fracture zones - Magmatic nature of crust supports a mechanism where transform crust is augmented before being turned into a fracture zon