Improvement of common bacterial blight resistance in South African dry bean cultiver teebud

Common bacterial blight (CBB) caused by Xanthomonas axonopodis pv. phaseoli is an important seed-borne disease of dry beans in South Africa.  Development of resistant cultivars is considered the best control measurement for the disease. Backcross breeding was used to improve  BB resistance in the small white canning bean, cv. Teebus, using resistance sources XAN 159 and Wilk 2. High resistance levels in near-isogenic lines, developed in two independent breeding programmes, indicated successful  transfer of resistance from both sources. Presence of SCAR-markers, SU91 and BC420, in 35 of 39 XAN 159 derived Teebus lines and all lines derived from Wilk 2, confirmed successful resistance transfer. AFLP studies conducted to determine genetic relatedness of two near-isogenic Teebus lines, showed a similarity of 96.2% with the maximum similarity between  these lines and Teebus being 93.1%. One cultivar, Teebus-RCR2 with yield similar to Teebus and improved resistance to CBB has been released from the programme

Improvement of common bacterial blight resistance in South African dry bean cultivar teebus

Common bacterial blight (CBB) caused by Xanthomonas axonopodis pv. phaseoli is an important seed-borne disease of dry beans in South Africa. Development of resistant cultivars is considered the best control measurement for the disease. Backcross breeding was used to improve CBB resistance in the small white canning bean, cv. Teebus, using resistance sources XAN 159 and Wilk 2. High resistance levels in near-isogenic lines, developed in two independent breeding programmes, indicated successful transfer of resistance from both sources. Presence of SCAR-markers, SU91 and BC420, in 35 of 39 XAN 159 derived Teebus lines and all lines derived from Wilk 2, confirmed successful resistance transfer. AFLP studies conducted to determine genetic relatedness of two near-isogenic Teebus lines, showed a similarity of 96.2% with the maximum similarity between these lines and Teebus being 93.1%. One cultivar, Teebus-RCR2 with yield similar to Teebus and improved resistance to CBB has been released from the programme.Le fl\ue9trissement bact\ue9rien commun (CBB) caus\ue9 par Xanthomonas axonopodis pv. phaseoli est une importante maladie de grains des haricots secs en Afrique du Sud. Le d\ue9veloppement des cultivars r\ue9sistants est consid\ue9r\ue9 comme une meilleure mesure de contr\uf4le de la maladie. L\u2019am\ue9lioration par croisement en retour \ue9tait utilis\ue9e pour am\ue9liorer la r\ue9sistance au CBB dans le petit haricot blanc, cv.Teebus, en utilisant des sources r\ue9sistantes XAN 159 et Wilk 2. De niveaux \ue9lev\ue9s de r\ue9sistance dans des lign\ue9es proches isog\ue9niques, d\ue9velopp\ue9es dans deux programmes ind\ue9pendants d\u2019am\ue9lioration, ont indiqu\ue9 un transfert r\ue9ussi de r\ue9sistance de toutes ces deux sources. De la pr\ue9sence des marquers SCAR, SU91 et BC 420 dans 35 de 39 XAN 159 a d\ue9coul\ue9 les lign\ue9es Teebus et toutes les lign\ue9es d\ue9rivant de Wilk 2, confirmant ainsi un transfert r\ue9ussi de r\ue9sistance. Des \ue9tudes AFLP conduites pour d\ue9terminer la relation g\ue9n\ue9tique de deux lign\ue9es proches isog\ue9niques Teebus, ont montr\ue9 une similarit\ue9 de 96.2% avec la maximum de similarit\ue9 entre ces lign\ue9es et Teebus de 93.1%. Un cultivar, TeebusRCR2 avec rendement similaire au Teebus et r\ue9sistance am\ue9lior\ue9e au CBB a \ue9t\ue9 dissemin\ue9e par le programme

Improvement of common bacterial blight resistance in South African dry bean cultiver teebus

Common bacterial blight (CBB) caused by Xanthomonas axonopodis pv. phaseoli is an important seed-borne disease of dry beans in South Africa. Development of resistant cultivars is considered the best control measurement for the disease. Backcross breeding was used to improve BB resistance in the small white canning bean, cv. Teebus, using resistance sources XAN 159 and Wilk 2. High resistance levels in near-isogenic lines, developed in two independent breeding programmes, indicated successful transfer of resistance from both sources. Presence of SCAR-markers, SU91 and BC420, in 35 of 39 XAN 159 derived Teebus lines and all lines derived from Wilk 2, confirmed successful resistance transfer. AFLP studies conducted to determine genetic relatedness of two near-isogenic Teebus lines, showed a similarity of 96.2% with the maximum similarity between these lines and Teebus being 93.1%. One cultivar, Teebus-RCR2 with yield similar to Teebus and improved resistance to CBB has been released from the programme

Improvement of common bacterial blight resistance in South African dry bean cultivar teebus

Common bacterial blight (CBB) caused by Xanthomonas axonopodis pv. phaseoli is an important seed-borne disease of dry beans in South Africa. Development of resistant cultivars is considered the best control measurement for the disease. Backcross breeding was used to improve CBB resistance in the small white canning bean, cv. Teebus, using resistance sources XAN 159 and Wilk 2. High resistance levels in near-isogenic lines, developed in two independent breeding programmes, indicated successful transfer of resistance from both sources. Presence of SCAR-markers, SU91 and BC420, in 35 of 39 XAN 159 derived Teebus lines and all lines derived from Wilk 2, confirmed successful resistance transfer. AFLP studies conducted to determine genetic relatedness of two near-isogenic Teebus lines, showed a similarity of 96.2% with the maximum similarity between these lines and Teebus being 93.1%. One cultivar, Teebus-RCR2 with yield similar to Teebus and improved resistance to CBB has been released from the programme.Le flétrissement bactérien commun (CBB) causé par Xanthomonas axonopodis pv. phaseoli est une importante maladie de grains des haricots secs en Afrique du Sud. Le développement des cultivars résistants est considéré comme une meilleure mesure de contrôle de la maladie. L’amélioration par croisement en retour était utilisée pour améliorer la résistance au CBB dans le petit haricot blanc, cv.Teebus, en utilisant des sources résistantes XAN 159 et Wilk 2. De niveaux élevés de résistance dans des lignées proches isogéniques, développées dans deux programmes indépendants d’amélioration, ont indiqué un transfert réussi de résistance de toutes ces deux sources. De la présence des marquers SCAR, SU91 et BC 420 dans 35 de 39 XAN 159 a découlé les lignées Teebus et toutes les lignées dérivant de Wilk 2, confirmant ainsi un transfert réussi de résistance. Des études AFLP conduites pour déterminer la relation génétique de deux lignées proches isogéniques Teebus, ont montré une similarité de 96.2% avec la maximum de similarité entre ces lignées et Teebus de 93.1%. Un cultivar, TeebusRCR2 avec rendement similaire au Teebus et résistance améliorée au CBB a été disseminée par le programme

GC-MS-based urinary organic acid profiling reveals multiple dysregulated metabolic pathways following experimental acute alcohol consumption

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Screening for inborn errors of metabolism among mentally retarded patients - Outcome of a survey at the Witrand Care and Rehabilitation Centre

The prevalence of different types of inborn errors of metabolism among the mentally retarded patients at the Witrand Care and Rehabilitation Centre. were determined by means of a biochemical screening survey. These results are compared with those of other surveys in South Africa and abroad. One important result points to substantial differences in the recorded incidences of metabolic defects between surveys. This observation could partially be due to significant differences between the different studies in terms of methodology employed and sampling procedures. The questions raised in this regard are documented and discussed

AFLPs are incompatible with RAPD and morphological data in Pennisetum purpureum (Napier grass)

Accessions of Pennisetum purpureum in agricultural research centres in sub-Saharan Africa were genotyped using Amplified Fragment Length Polymorphisms (AFLPs) using primer combinations MluI/MseI. Cluster analyses of resulting bands suggested that the samples could be grouped into five large and five small-sized clusters. Principal coordinate analysis revealed two major clusters being well separated, with bulk of the samples forming one, poorly structured cluster. Our analyses failed to reveal the regional groupings found in previous studies using a subset of our sample universe and based on RAPDs as well as morphological and agronomical data. The inability of the AFLP data to clearly demarcate accessions is ascribed to either the presence of high levels of genetic uniformity, or nonoptimal primers and/or bad germplasm management practices. The re-registration of all accessions, based on DNA barcoding, is suggested as a means to resolve the lingering problems regarding the identity of accessions