Similar cellular responses after treatment with either praziquantel or oxamniquine in Schistosoma mansoni infection.

The effect of treatment with either oxamniquine or praziquantel on S.mansoni specific IFN-gamma, IL-4, IL-5 and IL-10 was compared on PBMC which were collected pretreatment, 6 and 18 weeks post treatment. Using sandwich ELISA on the supernatants harvested from the PBMC stimulation by crude S. mansoni SEA and SWAP antigens after 5 days the levels of PBMC proliferation and cytokine production were similar according to treatment with either praziquantel or oxamniquine. Before treatment, infected groups showed low ratios, of IL-4:IFN-gamma, IL-5:IFNgamma and IL-10:IFN-gamma, indicating that IFN-gamma was high in the infected individuals. The general increase in immuno-modulation was observed post-treatment with elevated immune reactivity and cytokine production in both treatment groups. Treatment induced significant increases in levels of IL-4 (p < 0.05), IL-5 (p < 0.0001) and IL-10 (p < 0.05) cytokines 6 and 18 weeks after treatment. There were no significant differences in the increase in IL-4, IL-5 and IL-10 between children treated with praziquantel or oxamniquine. Pre-treatment IFN-gamma and IL-5 levels were positively correlated with infection (p < 0.001), while post treatment IL-4 cytokine levels were negatively correlated with baseline infection status (p < 0.001). The results suggest that treatment-induced immune responses are similar for both common anti-schistosome drugs praziquantel or oxamniquine having similar and immunizing effect

Immunological consequences of antihelminthic treatment in preschool children exposed to urogenital schistosome infection

Urogenital schistosomiasis, due to Schistosoma haematobium, is endemic in sub-Saharan Africa. Control is by targeted treatment with praziquantel but preschool age children are excluded from control programs. Immunological studies on the effect of treatment at this young age are scarce. In light of studies in older individuals showing that praziquantel alters antischistosome immune responses and responses to bystander antigens, this study aims to investigate how these responses would be affected by treatment at this young age. Antibody responses directed against schistosome antigens, Plasmodium falciparum crude and recombinant antigens, and the allergen house dust mite were measured in children aged 3 to 5 years before and 6 weeks after treatment. The change in serological recognition of schistosome proteins was also investigated. Treatment augmented antischistosome IgM and IgE responses. The increase in IgE responses directed against adult worm antigens was accompanied by enhanced antigen recognition by sera from the children. Antibody responses directed against Plasmodium antigens were not significantly affected by praziquantel treatment nor were levels of allergen specific responses. Overall, praziquantel treatment enhanced, quantitatively and qualitatively, the antiworm responses associated with protective immunity but did not alter Plasmodium-specific responses or allergen-specific responses which mediate pathology in allergic disease

Schistosome Infection Intensity Is Inversely Related to Auto-Reactive Antibody Levels

Acquired immunity against helminths is characterised by a complex interplay between the effector Th1 and Th2 immune responses and it slowly manifests with age as a result of cumulative exposure to parasite antigens. Data from experimental models suggest that immunity is also influenced by regulatory T cells (Treg), but as yet studies on Treg in human schistosome infections are limited. This study investigated the relationship between schistosome infection intensity and the two cell populations regulatory T cells (TREG: CD4(+(dim))CD25(+(high))FOXP3(+)CD127(low)), and activated (Tact: CD4(+)CD25(+)FOXP3(-)) T cells in Zimbabweans exposed to Schistosoma haematobium parasites. Participants were partitioned into two age groups, young children (8-13 years) in whom schistosome infection levels were rising to peak and older people (14+ years) with declining infection levels. The relationship between Tact proportions and schistosome infection intensity remained unchanged with age. However Treg proportions rose significantly with increasing infection in the younger age group. In contrast Treg were negatively correlated to infection intensity in the older age group. The relative proportions of regulatory T cells differ significantly between young individuals in whom high infection is associated with an enhanced regulatory phenotype and older infected patients in whom the regulatory response is attenuated. This may influence or reflect different stages of the development of protective schistosome acquired immunity and immunopathogenesis

Soluble CD23 Levels are Inversely Associated with Atopy and Parasite-Specific IgE Levels but Not with Polyclonal IgE Levels in People Exposed to Helminth Infection

BACKGROUND: Protective acquired immunity against helminths and allergic sensitisation are both characterised by high IgE antibody levels. Levels of IgE antibodies are naturally tightly regulated by several mechanisms including binding of the CD23 receptor. Following observations that helminth infections and allergic sensitisation may co-present, the current study aims to investigate the relationship between the soluble CD23 (sCD23) receptor, parasite-specific IgE responses and allergic sensitisation in people exposed to the helminth parasite Schistosoma haematobium. METHODS: A cohort of 434 participants was recruited in two villages with different levels of S. haematobium infection in Zimbabwe. Serum levels of the 25-kDa fragment of sCD23 were related to levels of schistosome infection intensity, allergen (house dust mite, HDM) and schistosome-specific IgE, total IgE and skin sensitisation to HDM. RESULTS: sCD23 levels rose significantly with schistosome infection intensity but declined significantly with schistosome-specific IgE levels. Furthermore, sCD23 levels were negatively associated with skin sensitisation and IgE reactivity against HDM, but showed no relationship with total IgE. CONCLUSION: The results are consistent with the suppression of parasite and allergen-specific IgE levels by sCD23. Further mechanistic studies will determine the relevance of this potential regulatory mechanism in the development of helminth-specific immune responses in atopic individuals

Differences in the faecal microbiome in Schistosoma haematobium infected children vs. uninfected children

BACKGROUND: Several infectious diseases and therapeutic interventions cause gut microbe dysbiosis and associated pathology. We characterised the gut microbiome of children exposed to the helminth Schistosoma haematobium pre- and post-treatment with the drug praziquantel (PZQ), with the aim to compare the gut microbiome structure (abundance and diversity) in schistosome infected vs. uninfected children. METHODS: Stool DNA from 139 children aged six months to 13 years old; with S. haematobium infection prevalence of 27.34% was extracted at baseline. 12 weeks following antihelminthic treatment with praziqunatel, stool DNA was collected from 62 of the 139 children. The 16S rRNA genes were sequenced from the baseline and post-treatment samples and the sequence data, clustered into operational taxonomic units (OTUs). The OTU data were analysed using multivariate analyses and paired T-test. RESULTS: Pre-treatment, the most abundant phyla were Bacteroidetes, followed by Firmicutes and Proteobacteria respectively. The relative abundance of taxa among bacterial classes showed limited variation by age group or sex and the bacterial communities had similar overall compositions. Although there were no overall differences in the microbiome structure across the whole age range, the abundance of 21 OTUs varied significantly with age (FDR<0.05). Some OTUs including Veillonella, Streptococcus, Bacteroides and Helicobacter were more abundant in children ≤ 1 year old compared to older children. Furthermore, the gut microbiome differed in schistosome infected vs. uninfected children with 27 OTU occurring in infected but not uninfected children, for 5 of these all Prevotella, the difference was statistically significant (p <0.05) with FDR <0.05. PZQ treatment did not alter the microbiome structure in infected or uninfected children from that observed at baseline. CONCLUSIONS: There are significant differences in the gut microbiome structure of infected vs. uninfected children and the differences were refractory to PZQ treatment

The acceptability of insecticide treated mosquito nets among community members in Zimbabwe

A CAJM article on the advocacy of using treated mosquito nets in rural communities.Objectives: To assess the acceptability of insecticide treated mosquito nets (ITNs) among community members in Zimbabwe for the implementation of effective malaria intervention using ITNs. Design: A cross sectional study and longitudinal study were carried out using questionnaires, which were administered by health workers from rural health centres. Setting: Communities in rural areas from seven districts namely Bulilimamangwe, Chipinge, Gokwe, Hurungwe, Lupane, Mount Darwin and Uzumba Maramba Pfungwe (UMP) in Zimbabwe. Subjects: 1 576 community members who bought ITNs in the project areas. Main Outcome Measure: Acceptance of ITNs and price, reasons for wanting or not wanting nets; ITNs usage and washing pattern among respondents. Results: More than 90% of people said they bought the nets from the project in order to avoid mosquito bites and to prevent malaria. Quite a number of respondents (69%) gave the answer that they bought mosquito nets from the project because they were cheaper (Z$130 to Z$390) than the market price (Z$l 000) and more than half (58.3%) claimed that they could not afford to buy mosquito nets at the market price. The price was accepted by the majority as cheap or very cheap in Gokwe, Hurungwe, Mount Darwin, and UMP (92%, 82%, 98% and 90%, respectively). Other responses given for having bought nets were at health staff’ s-recommendation (71.3 %), and they were suffering from malaria (40.9%). The reasons for not having bought mosquito nets were that they had not suffered from malaria (55.5%), that they did not like to use a mosquito net (31.1%), did not know the efficacy of the net (21.6%), or there were not many mosquitoes at home (28.9%). The percentage of ITN use was very high (90 to 100%) amongst those who bought nets. The percentage of children under five years and pregnant women who were ITN users ranged from six to 24% to 2.8 to 9.7%, respectively. Percentage of “others” which means those above five years and not pregnant was more than 70% in all the districts. The washing pattern of ITNs in Chipinge showed that most of the mosquito nets (74.5%) were washed every three to five months. Conclusion: Acceptability of ITNs was very high based on reported utilisation of ITNs. It is recommended that rural communities should be sensitised on the importance of treated nets for malaria prevention, and advised to purchase mosquito nets whenever cash is readily available in each community

PROVISION OF QUALITY EDUCATION IN PRIVATE SCHOOLS: REFLECTIVE PRACTICES IN LOW-COST PRIVATE SECONDARY SCHOOLS IN BULAWAYO METROPOLITAN PROVINCE, ZIMBABWE

Quality in education has become a cause for concern to every stakeholder in education. The study sought to assess the provision of quality education in low-cost private secondary schools in Bulawayo Metropolitan Province. The study adopted the interpretivist paradigm in qualitative approach, using a case study design. Semi-structured interviews and document analysis were used for collecting thematically analysed data from purposively sampled four school heads and six teachers from low-cost private secondary schools. The study established that in pursuit for quality education, selected schools employed qualified teachers who engage in continuous professional development programmes to sharpen their teaching skills. The findings revealed that the schools understudy are making efforts to offer practical science and computer lessons using the limited resources to ensure the provision of quality education. It emerged that selected schools use e-learning and multimedia resources which arouse learners’ interests and increase the retention rates. It came out that the schools understudy have environmental clubs which work together with school health departments in attending to sanitary issues. Whilst selected schools practiced heterogeneous grouping, the findings revealed that learners with physical disabilities are not enrolled in those schools due to lack of appropriate physical facilities and there are no teachers with relevant expertise to teach learners with special needs. The study revealed that the Ministry of Primary and Secondary Education provided guidelines in the form of policy documents and circulars to monitor the provision of quality education in the selected schools. Despite the efforts made by low-cost secondary schools in providing quality education, the study found that high staff turnover is negatively affecting the quality of education due to lack of continuity in learning. The findings indicated that inadequate learning resources and infrastructure such as libraries, computer and science laboratories, internet services, and lack of teachers with special needs expertise adversely affected the provision of quality education. The study concludes that lack of financial resources is a hindrance in the provision of quality education in low-cost secondary schools. The study recommends that a comparative study on provision of quality education should be conducted in private trust secondary schools. Article visualizations

The effects of Zimbabwe’s economic crises on child welfare: a case of Chitungwiza Town

This study examined the effects of Zimbabwe’s economic crisis on the welfare of children (analysed in terms of two indicators of health and education) in Chitungwiza. The study endeavoured to achieve the following specific objectives: (i).to assess the effect of Zimbabwe’s economic crisis on the welfare of children in Chitungwiza, Zimbabwe. (ii). to examine child support strategies that are implemented to sustain children during economic crisis in Chitungwiza, Zimbabwe. The economic exigencies and shocks spawned by economic crisis on the weak welfare systems of the developing world countries drastically undermine the wellbeing of children. Therefore, this study focused on analysing the economic crises-spawned form and scale of child vulnerabilities at the household and national level because these units provide primary child care in Zimbabwe. This study was underpinned by two frameworks namely, the Social Risk Management Approach (SRMA) and the Transformative Social Protection Framework (T.S.P). The study used a mixed methods or triangulation approach, through use of primary data (quantitative and qualitative data) and secondary data (archival data) as the basis for data collection as well as the analysis. This was guided by a mixed research design specifically a case study and a mini survey. Complementarity of the research methods was achieved through the use of data from household surveys with 100 heads of households (selected through a combination cluster and random sampling) in Chitungwiza Town and in-depth interviews with 1 official from each of the following units; Ministry of Primary and Secondary Education, the Ministry of Public Service Labor, and Social Welfare, Ministry of Health and Child care, UNICEF, the National Social Security Authority (NSSA) and Child Line Zimbabwe (selected through a purposive sampling technique). Data was analyzed qualitatively through thematic analysis, while descriptive statistics was used to analyze quantitative data through the use of SPSS (Statistical Package for Social Sciences, Version 25) and Microsoft Excel 2007. The study revealed that there are so many child vulnerabilities entrenched by the country’s chronic economic crisis that include poor education and health outcomes, drug abuse and juvenile delinquency, early pregnancies and child marriages, child labour and exploitation among others. Social safety nets in place such as BEAM, AMTO and HSCT are to some extent helping vulnerable children but currently these are not covering a significant percentage of children from poor households due to budget, capacity and fund appropriation challenges at the behest of the country’s economic crisis. Household child support strategies such as smallholder agriculture and gardening, informal trading and jobs are to some extent helping in promoting child wellbeing but their potential is undercut by challenges relating to inter alia the hyperinflationary economic environment, regulation and suppressed markets. The study inter alia recommends the institution of transparency and accountability as provided in the 2013 constitution to curb leakages and misallocation of the few resources, the reinstatement of the multicurrency monetary system in order to stabilise the country’s hyperinflationary environment, a transformation of the delivery of social safety nets in terms of targeting and coverage of the poor households, more effective budgeting and resource allocation system, supporting the informal sector and enhanced integration of social work practise in government social protection programs.Thesis (MSW) -- Faculty of Social Sciences and Humanities, 202

The effects of Zimbabwe’s economic crises on child welfare: a case of Chitungwiza Town

This study examined the effects of Zimbabwe’s economic crisis on the welfare of children (analysed in terms of two indicators of health and education) in Chitungwiza. The study endeavoured to achieve the following specific objectives: (i).to assess the effect of Zimbabwe’s economic crisis on the welfare of children in Chitungwiza, Zimbabwe. (ii). to examine child support strategies that are implemented to sustain children during economic crisis in Chitungwiza, Zimbabwe. The economic exigencies and shocks spawned by economic crisis on the weak welfare systems of the developing world countries drastically undermine the wellbeing of children. Therefore, this study focused on analysing the economic crises-spawned form and scale of child vulnerabilities at the household and national level because these units provide primary child care in Zimbabwe. This study was underpinned by two frameworks namely, the Social Risk Management Approach (SRMA) and the Transformative Social Protection Framework (T.S.P). The study used a mixed methods or triangulation approach, through use of primary data (quantitative and qualitative data) and secondary data (archival data) as the basis for data collection as well as the analysis. This was guided by a mixed research design specifically a case study and a mini survey. Complementarity of the research methods was achieved through the use of data from household surveys with 100 heads of households (selected through a combination cluster and random sampling) in Chitungwiza Town and in-depth interviews with 1 official from each of the following units; Ministry of Primary and Secondary Education, the Ministry of Public Service Labor, and Social Welfare, Ministry of Health and Child care, UNICEF, the National Social Security Authority (NSSA) and Child Line Zimbabwe (selected through a purposive sampling technique). Data was analyzed qualitatively through thematic analysis, while descriptive statistics was used to analyze quantitative data through the use of SPSS (Statistical Package for Social Sciences, Version 25) and Microsoft Excel 2007. The study revealed that there are so many child vulnerabilities entrenched by the country’s chronic economic crisis that include poor education and health outcomes, drug abuse and juvenile delinquency, early pregnancies and child marriages, child labour and exploitation among others. Social safety nets in place such as BEAM, AMTO and HSCT are to some extent helping vulnerable children but currently these are not covering a significant percentage of children from poor households due to budget, capacity and fund appropriation challenges at the behest of the country’s economic crisis. Household child support strategies such as smallholder agriculture and gardening, informal trading and jobs are to some extent helping in promoting child wellbeing but their potential is undercut by challenges relating to inter alia the hyperinflationary economic environment, regulation and suppressed markets. The study inter alia recommends the institution of transparency and accountability as provided in the 2013 constitution to curb leakages and misallocation of the few resources, the reinstatement of the multicurrency monetary system in order to stabilise the country’s hyperinflationary environment, a transformation of the delivery of social safety nets in terms of targeting and coverage of the poor households, more effective budgeting and resource allocation system, supporting the informal sector and enhanced integration of social work practise in government social protection programs.Thesis (MSW) -- Faculty of Social Sciences and Humanities, 202

Cadmium and lead concentrations in livers and kidneys of cattle slaughtered at Grootfontein abattoir in Namibia

The aim of this study was to determine the levels of cadmium (Cd) and lead (Pb) in livers and kidneys of cattle slaughtered at Grootfontein abattoir in Namibia. The study design was based on the epidemiological principles to detect a single animal whose kidneys or liver contained Cd or Pb residues. The Grootfontein area of Namibia has extensive base-metal ore reserves, which were and are still extracted and processed in localities used as livestock pastures. Namibia is also an arid country which predominantly uses borehole water for livestock and human consumption. These underground water bodies share the same space as base-metal ores. The anthropogenic activities in this area under the existing geological and hydrogeological circumstances offer opportunities for Cd and Pb to enter the food chain. Entry of Cd and Pb in the food chain leads to bioaccumulation in cattle kidneys and livers to concentrations above Codex Alimentarius Commission (CAC) standards, creating a possible public health risk. The CAC withdrew the maximum limit (ML) of 1mg kg-1 Cd in bovine kidneys and liver, but it has a provisional tolerable monthly intake (PTMI) of 0.025mg kg-1 human body weight. This CAC PTMI translates to a total exposure of 1.5mg Cd for a 60kg body weight person. The CAC ML for Pb in bovine offal is 0.5mg kg-1, while its provisional tolerable weekly intake (PTWI) of 0.025mg kg-1 human body weight is under review. This investigation intended to establish if Cd and Pb in the livers and kidneys of cattle slaughtered in the study area exceeded CAC human exposure limits. Liver and kidney specimens were collected from 31 randomly sampled mature cattle (estimated over five years old based on incisor teeth examined post slaughter). The specimens were analysed at a local mine laboratory, which was the only facility available and capable of performing the tests. They were digested using wet-ashing (the oxidation procedure). All liver digestates were analysed, while one kidney analyte was insufficient. Cd and Pb were measured using flame atomic absorption spectroscopy (FAAS). The detection limit (DL), which was the minimum metal concentration FAAS could measure was 0.2mg kg-1 for Cd and 1.1mg kg-1 for Pb. The laboratory could not refine the Pb DL which was more than twice the CAC ML. All livers had Cd concentrations below 0.2mg kg-1. One discarded kidney specimen was assigned a concentration below DL for analysis purposes. The Cd concentrations in 12 kidney specimens were below 0.2mg kg-1, between 0.288 and 1.221mg kg-1 in 16 and above 1.5mg kg-1 (2.6 - 3.64mg kg-1) in 3 specimens. The mean renal Cd concentration for the population (0.71±0.96mg kg-1) was statistically lower than 1.5mg kg-1 (p 5 years) and it is likely that this factor increased the risk of higher levels in kidneys as temporal determinants for bioaccumulation of Cd are important. The kidneys of younger animals would thus be less likely to contain significant Cd levels. Lastly, use of mathematical modelling, to translate research findings into quantitative estimates useful for public health safety programs, is recommended.Dissertation (MMedVet)--University of Pretoria, 2012.Paraclinical Sciencesunrestricte