Amino acid sequence of heat-stable enterotoxin produced by Vibrio cholerae non-01

AbstractThe amino acid sequence of heat-stable enterotoxin, produced by Vibrio cholerae non-01 and isolated from its culture supernatant, was determined by both Edman degradation of native and reductively carboxy-methylated enterotoxin and also a combination of fast atom bombardment mass spectrometry and carboxy-peptidase Y digestion of native enterotoxin to be as follows: Ile-Asp-Cys-Cys-Glu-Ile-Cys-Cys-Asn-Pro-Ala-Cys-Phe-Gly-Cys-Leu-Asn. This sequence is very similar, but not identical, to those of heat-stable enterotoxins produced by enterotoxigenic Escherichia coli and Yersinia enterocolitica

<CLINICAL REPORT>A case of inverted papilloma of the maxillary sinus

A case of inverted papilloma arising in the maxillary sinus is described. The first symptoms were rhinorroea and discomfort of the left infraorbital region. A biopsy obtained by penetration into the maxillary sinus resulted in a diagnosis of chronic sinusitis. However, the surgical specimen showed characteristic inverted epithelial proliferation. Two years after operation by the Caldwell-Luc method, there are no signs of recurrence. The diagnosis and treatment of inverted papilloma are discussed

ニンチショウ コウレイシャ ノ ショクジセイ テイケツアツ ト ジリツ シンケイ カツドウ ト ノ カンレン

【目的】認知症高齢者の食事性低血圧(PPH)と自律神経活動の関連と,PPHの発現頻度,自律神経活動との関連を明らかにする.【方法】歩行可能な入院中の認知症高齢者を対象に,食前と食後の血圧,脈拍,指尖脈波を用いた自律神経活動測定を,座位にて5日間実施した,【結果】対象者は男性11人,女性20人,合計31人で,平均年齢85.42±6.36歳であった.病名別ではアルツハイマー型認知症(AD)23人,レビー小体型認知症(DLB)6人,ウェルニッケ･コルサコフ症候群1人,脳血管性認知症1人であった.対象者の61%にPPHの発現があった.ADの70%にPPHの発現があった.PPH発現時のLn(LF/HF)は食前0.93±0.24,食後0.81±0.17(p＜0.05)で有意差があった.PPHの発現なし時のLn(LF/HF)の平均値は食前0.93±0.28,食後0.92±0.26であり,有意差はなかった.【考察】PPH発現のケースでは食前･後のLn(LF/HF)に差があり,PPH発現なしではLn(LF/HF)に差がないことから,PPHの発現ケースには心臓交感神経活動が食前と比して食後に有意に減弱しており,交感神経活動にPPHが影響していることが示唆された.Purpose : This study aims to clarify the correlation between postprandial hypotension (PPH) and autonomicnervous activity in elderly people with dementia.Method : We examined the autonomic nervous activities of ambulatory patients by measuring their blood pressure, pulse and finger plethysmogram before and after meals for five days.Results : There were 11 male and 20 female subjects with a median age of 85.42 ± 6.36. We had 23 patientswith AD, six with DLB, one with Wernicke-Korsakoff, and one with cerebrovascular dementia. 61 percent of the subjects showed symptoms of PPH. 70 percent of the patients with AD showed symptoms of PPH. The value of Ln (LF / HF)with PPH was 0.93 ± 0.24 before meal and 0.81 ± 0.17 (p<0.05) after meal, while the average value of Ln(LF / HF)without PPH was 0.93 ± 0.28 before meal and 0.92 ± 0.26 after meal. The comparison showed no significant differences.Discussion : No disparity was found in autonomic nervous activities among patients without PPH symptoms.It is suggested that PPH affects sympathetic nerve activity since we observed a significant decrease of sympathetic nerve activities after meals

Vitamin K deficiency, evaluated with higher serum ucOC, was correlated with poor bone status in women

BACKGROUND: An increase in serum undercarboxylated osteocalcin concentrations suggests vitamin K deficiency. Clinical intervention studies suggested that the vitamin K supplementation might contribute to preventing bone loss in postmenopausal women. Evidence on the relationship between serum undercarboxylated osteocalcin (ucOC) levels and bone parameters of quantitative ultrasound (QUS) is limited. We examined the correlation between serum ucOC concentrations and bone status as measured by QUS among middle-aged and older Japanese men and women. METHODS: The subjects were community-dwelling men (n = 358) and women (n = 503) aged ? 40?years in Japan. Heel QUS parameters, including the stiffness index, speed of sound, and broadband ultrasound attenuation, were measured. Serum ucOC concentrations were measured by electrochemiluminescence immunoassay. Grip strength was measured in the dominant hand. Information on alcohol drinking, current smoking, exercise, and menopause in women was collected. RESULTS: Serum ucOC concentrations were significantly associated with age in both sexes. Serum ucOC concentrations in men were higher at ? 80?years than those in the age groups of 40-49, 50-59, and 60-69?years. Serum ucOC concentrations in women were higher in the age groups of 50-59 and 60-69?years than those at 40-49?years. Partial correlation analysis adjusting for covariates (age, body mass index, grip strength, alcohol drinking, current smoking, and exercise in men; age, body mass index, grip strength, alcohol drinking, current smoking, exercise, and menopause in women) showed that serum ucOC concentrations were negatively significantly correlated with all QUS parameters in women. Serum ucOC concentrations were not correlated with them in men. CONCLUSIONS: Vitamin K deficiency, evaluated with higher serum ucOC, was correlated with poor bone status in women