Health Sciences University of Hokkaido Academic Repository / 北海道医療大学学術リポジトリ

Youjing  Qiu

北海道医療大学学術リポジトリ象牙質再生におけるC C N 2 のインビトロ研究著者 Youjing  Qiu学位名 博士（歯学）学位授与機関 北海道医療大学学位授与年度 平成30年度学位授与番号 30110甲第312号URL http://id.nii.ac.jp/1145/00064682/論 文 要 旨 象 牙 質 再 生 に お け る C C N 2 の イ ン ビ ト ロ 研 究                 平 成 3 0 年 度 北 海 道 医 療 大 学 大 学 院 歯 学 研 究 科  邱  友 靖  Abstract T h e  i n  v i t ro  s t u d y  o f  C C N 2  o n  d e n t i n  r e g e n e r a t i o n               2019 Graduate School of Dentistry, Health Sciences University of Hokkaido Youjing QIU [Introduction] Direct pulp capping has been used for the treatment of pulp exposed by deep dental caries or traumatic injury. Under conditions of severe damage, newly differentiated odontoblast-like cells may migrate to sites of injury, where they are thought to form reparative dentin that protects pulp tissue from stimuli. Connective tissue growth factor/Cysteine-rich 61/Nephroblastoma overexpressed family 2 (CCN2) plays complex roles in various biological processes such as embryogenesis, angiogenesis, chondrogenesis, wound healing, tissue regeneration, etc. However, its effects in dentin regeneration remain unclear. In this study, we aimed to investigate the in vitro effects of CCN2 on odontoblast-like cells and human dental pulp stem cells (hDPSCs) in proliferation, mineralization and differentiation, as well as its potential application in dental treatment. [Materials and methods] MDPC-23 cells, a type of rat odontoblast-like cell line, were cultured in DMEM supplemented with 5% FBS. hDPSCs were cultured in DMEM containing 10% FBS, 50 units/mL penicillin and 50μg/mL streptomycin. This study was divided into three independent experiments. In experiment 1, soluble CCN2 (100 ng/mL) was added into culture media; in experiment 2 and 3, CCN2 at the concentration of 1000 ng/mL was coated onto culture polystyrene, addition or coating of distilled water was served as a control. Cell proliferation was assessed using the CCK-8 assay. The cytoskeleton was stained using Alexa Fluor 568-labeled phalloidin and visualized by fluorescence microscopy. Cell differentiation and mineralization were analyzed by ALPase activity assay, real time RT-PCR and alizarin red staining. Data were analyzed using the post hoc Tukey honest significance difference test. [Results] *Experiment 1 Cell proliferation of MDPC-23 cells was promoted by the addition of soluble CCN2. Gene expression of rBSP, rOCN and rOPN were promoted after 48h exposure to soluble CCN2. Mineralization of MDPC-23 cells was accelerated by the addition of soluble CCN2. *Experiment 2 CCN2 promoted MDPC-23 cells proliferation in a dose-dependent manner, with the greatest effect observed at 1000 ng/mL. ALPase activity of cells cultured on CCN2-modified surfaces continuously strengthened from day six (0.83±0.02 units/µg protein versus 0.56±0.01 units/µg protein of control) until day eight (1.04±0.14 units/µg protein versus 0.70±0.06 units/µg protein of control). Moreover, gene expression of rBSP, rOCN, rOPN, rALP, rCOL1A1, rRunx-2, rDSPP, and rDMP-1 was significantly enhanced by the presence of immobilized CCN2. Finally, mineralization of MDPC-23 cells was markedly enhanced by CCN2. *Experiment 3 hDPSCs exhibited robust proliferative activity upon exposure to immobilized CCN2. Gene expression of hBSP, hCol1a1, and hALP in the CCN2-coated group was significantly higher than that of the control. The relative ALPase activity was also increased in the CCN2-coated group. Mineral nodule formation by hDPSCs grown on CCN2-coated surface was remarkably increased as compared to those grown on noncoated surfaces. [Discussion] Recently, ongoing studies have identified CCN2 protein as a growth and signaling factor that interacts with cell surface molecules and intracellular signaling pathways. Through its interaction with cell surface receptors, integrins, extracellular counterparts and extracellular matrixes, CCN2 governs inter- and intracellular communications to either directly or indirectly regulate cell proliferation and differentiation. The present study also indicated that the addition of 100 ng/mL CCN2 directly into culture media in a short culture period of 48h primarily promoted mineralization rather than odontogenic differentiation on MDPC-23 cells. [Conclusion] The findings indicate that CCN2 promotes proliferation, differentiation and mineralization of MDPC-23 and hDPSCs. It is proposed that CCN2 may be a promising adjunctive formula for dentin regeneration. 

English

象牙質再生におけるC C N 2 のインビトロ研究

サカモト マサキ

トダ カナエ

ヒダカ タツヒロ

フジワラ サヤカ

ヤマモト クニオ

Masaki Sakamoto

Kanae Toda

Tatsuhiro Hidaka

Sayaka Fujiwara

Kunio Yamamoto

北海道医療大学学術リポジトリProperties of an experimental prosthetic resincomposite containing finely powdered enamel著者名(英) Masaki Sakamoto, Kanae Toda, Tatsuhiro Hidaka,Sayaka Fujiwara, Kunio Yamamotojournal orpublication title北海道医療大学歯学雑誌volume 24number 1page range 113year 2005-06-30URL http://id.nii.ac.jp/1145/00009909/

Properties of an experimental prosthetic resin composite containing finely powdered enamel

北海道医療大学歯学会

北海道医療大学学術リポジトリ裏表紙著者 北海道医療大学歯学会journal orpublication title北海道医療大学歯学雑誌volume 35number 1year 2016-06-30URL http://id.nii.ac.jp/1145/00010521/Vol.35?No.1 ?????????Dent J Health Sci Univ HokkaidoDent? J?Health?SciUniv?Hokkaido???? ??? ??? ?? ??? ?? ????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? ????????? ? ????????????????????????????????????????????????????????????????????????????????????? ? ?? ? ? ? ? ???????????????????????????????????????????????????????????????????????????????? ????? ? ????? ????????????????????????A passage to SNAREs???????????????????? ?????????????????????????????????? ???????????????Evaluation of the bio-physiological efficacy of tooth storage media based on normal physiological ???parameters???????????????????????????????????????????????????????????????? ???????????? ????????Development of the fabrication method for a novel complete denture with a framework made of light-?????cured prosthetic hard resin?????????????????????????????????????????????? ???????????????????????????????Possible Association between Cigarette Smoking Habit with Periodontal Disease in Diabetic patients in ????Nepal????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????A interfacility collaboration case study of professional oral health care in ectodermal dysplasia?????????????????????????????? ????????????????????????????? ??????????????General Introduction of Dental Education in Universitas Indonesia?????????????????????????????????????????????????????????????? ?????????? ????????Oral Health and Dental Education in Nepal?????????????????????????????????????????????????????????????????? ?????? ?????????????????????????????????????????Effects of mechanical stress on expression of extracellular matrix in rat TMJ disc cells??????????????????? ??????????????????????????????????? ????????Relationship between facial asymmetry and bilateral differences of condylar morphology???-Three-dimensional (3D) morphological assessment-???????????????????? ?????????????????????????????????? ??????????????????????????Recent topics? ?????????????????????????????????????? ????6102311_H1_4.indd   1 2016/07/20   18:40:37

裏表紙

北海道医療大学学術リポジトリ投稿の手引き著者 北海道医療大学歯学会雑誌名 北海道医療大学歯学雑誌巻 35号 1発行年 2016-06-30URL http://id.nii.ac.jp/1145/00010518/「北海道医療大学歯学雑誌」投稿の手引き（2012年6月30日現在）本誌の体裁を統一するために，「投稿の手引き」に準拠して，ご執筆下さいますようお願い致します．原稿はすべてＡ4版とし，下記の項目1）～7）のすべてを，2部提出して下さい．査読後，論文掲載が認められた際には，論文原稿を収めたUSBメモリー（USBメモリーは，印刷終了後にお返しします）をプリントした最終原稿1部とともに提出して下さい．1）投稿原稿表紙2）チェックリストシート3）英文抄録4）本文5）文献6）図，表7）図表説明文1．投稿原稿表紙表紙には以下の事項を和文および英文で記入する．1）原稿の種類2）表題3）著者名および所属4）キーワード（5語以内）5）別刷数（50部単位）6）連絡先（郵便番号，住所，電話， e −mail）1）表題⑴ 一般固有名詞として通用していない商品名は用いない．⑵ 和文表題には，原則として略号以外の英文字を用いない．別にスペースも含めて35字以内のランニングタイトルを付ける．⑶ 英文表題は和文表題の内容と一致させる．文頭のみ大文字とし，他は小文字とする．また，別にスペースも含めて45字以内の英文ランニングタイトルを付ける．⑷ 副題はできる限り用いない．ただし，必要な場合は次の例に準拠する．続報，第2報などの表記は認めない．和文・英文：－□□□□□□□□□－2）著者名および所属⑴ 氏名の英文表記では，姓は大文字，名は先頭のみを大文字とする（例：Akira YAMADA（山田 昭））．⑵ 著者の所属が2ヶ所以上の場合には，所属の著者に 1），2），3） を付ける．3）キーワード5語以内のキーワードを付ける．英文の場合は，キーワードの先頭のみを大文字とし，他は小文字とする（例： Impression materials, Bone morphogeneticproteins）．2．チェックリストシートチェックリストの指示に従い，投稿原稿を確認する．著者全員のサインを取り，連絡先を記載する．3．英文抄録300語以内の英文抄録を付ける．4．本文1）原稿はA4判用紙（縦）にワードプロセッサなどによる横書きとする．書式は以下に従うこと．・Windows Microsoft Word余白は上下3cm，左右2．5cm文字は12ポイント1頁35文字×26行行間を1．5行句読点は「．」と「，」（全角）を用いる．英文の場合は，半角文字を使用する．・Macintosh Microsoft Word余白は上下3cm，左右2．5cm文字は12ポイント1頁30－35文字×22－25行行間を1．5行句読点は「．」と「，」（全角）を用いる．英文の場合は，半角文字を使用する．2）原稿の下段中央にページ番号を記す．3）論文の原則的な構成は，緒論（緒言），方法（材料および方法），結果，考察（結果および考察），結論（結語），謝辞，文献，図の説明，図表とする．4）見出しを用いるときは次の順に項目をたてる．3 → 3）→ ⑶ → a → a）→ ⒜5）文章は，専門用語を除いて，常用漢字，新かなづかい，ひらがなは口語体とする．6）数字はアラビア数字とし，単位の記号はJIS・Z8202およびZ8203に準じ，国際単位系（Sl）を使用するよう努める．また単位にピリオドをつけない．（例：GHz，MPa，kW，cm，mV，μm，nA，pF，mL，mmol，N（kgf），K，℃，min）7）学術用語は，原則として「文部省学術用語集」に準拠する．8）商品名，器械名などは，可能な限り一般化されている「カタカナ書き」とする．英文字で表す場合は，かしら文字のみ大文字にする．9）外国の人名などの固有名詞は原則として原綴とする．10）連続した数値は「，」でつなぎ，最後に単位をつける．（例：10，20，30℃）11）製造社の表記法は（ ）内に会社名のみを記し，社製および製作所，工業社製，株式会社などを入れない．例：（型式名，製造会社名），（略号，製造会社名）（X−3010，日立） （EPMA，日本電子）12）図表の挿入場所を本文右欄外に朱書きする．5．文献1）文献リストは，アルファベット順（A, B…Z順）で作成する．また本文中の引用箇所に以下の体裁に従い，文献内容を記載する．例：単著者（Izumi, 1999）（和泉，1999），2名（Izumi & Ito, 1998）（和泉，伊藤，1998），3名以上（Izumi et al. , 1970）（和泉ら，1970），2編以上（Sato et al., 1988; Izumi,93（93）第３５巻１号　　　４Ｃ１５０　１Ｃ１３３／本文　※３１‐１から組体裁変更　ＯＴＦ／０９３～０９５　投稿の手引き  2016.07.13 11.45.34  Page 93 1999）（佐藤ら，1988；和泉，1999）（Izumi,1999a, b）※「，」や「；」の様な記号は，日本文の場合は全角，英文の場合は半角を使用する．2）文献として不適当なもの，例えば未公表のデータや私信などは文献として引用しない．3）文献の著者または編集者が複数の場合にはet al.,他などとせず，その全部を記載する．4）著者名が欧字綴の場合は姓の後に名前の頭文字をつけ，また著者が複数の場合は最後の著者の前に&を入れる．※ 著者間の「and」は記号「&」を使用すること．5）文献の記載方法の基本は次のとおりとする．⑴ 雑誌の場合著者名（複数の場合，氏名を「，」で区切る．）．表題－サブタイトル－．雑誌名 巻：引用ページの始めと終わり，発行年．例：Izumi H, Ito Y, Sato M, Karita K & Iwat-suki N. The effects of inhalation anes-thetics on the parasympathetic reflexvasodilatation in the lower lip and pal-ate of the cat. Am J Physiol Regula-tory Integrative Comp Physiol 273: R168−R174, 1997.⑵ 単行本の場合ⅰ）章を参考にしたとき例：Weinstein L, Swartz MN. Pathologicproperties of invading microor-ganisms.In：Sodeman WA Jr, Sodeman WA, edi-tors. Pathologic physiology: mecha-nisms of disease. Philadelphia: Saun-ders, 1974, p457−472.ⅱ）個人または複数の著者の場合例：Colson JH, Armour WJ. Sports inju-ries and their treatment. 2nd ed.London: S. Paul; 1986.ⅲ）編集者，監修者が著者の場合例：Diener HC, Wilkinson M, editors.Drug − induced headache. NewYork: Springer−Verlag; 1988.ⅳ）団体，組織が著者で，かつ出版社の場合例：Virginia Law Foundation. The medicaland leagal implications of AIDS.Charlottesville: The Foundation;1987.ⅴ）会議録全体を参考にした場合例：Vivian VL, editor. Child abuse andneglect: a medical community re-sponse. Proceedings of the FirstAMA National Conference onChild Abuse and Neglect; 1984Mar 30 − 31; Chicago. Chicago:American Medical Association;1985.⑶ 分担執筆の場合分担執筆者名：分担執筆の表題．書名 巻など，発行所名：発行年，引用ページの始めと終わり．例：山田早苗：橋義歯の力学－傾斜歯ブリッジの形成と設計について－．新臨床歯科学講座3，医歯薬出版：1978，157－165．⑷ 翻訳書の場合著者（翻訳者）：書名（原著書名）．発行所名：発行年，引用ページの始めと終わり．例：Davidge RW（鈴木弘茂，井関孝善）：セラミックスの強度と破壊（Mechanicalbehavior of ceramics）．共立出版：1982，34−55．6．図1）用紙はＡ4版（縦）とし，1枚ずつ別葉にする．2）各葉杖に，図の番号，著者名，片段あるいは両段の指定，カラー印刷の有無を明記する．3）図の大きさは，片段か両段一杯になることがのぞましい．刷り上がりを想定して，図の大きさが片段で横幅45－68mm，両段で100－150mmになるように縮小コピーし，文字，記号の大きさ，線の太さなどをチェックする，棒グラフなどのハッチングは識別可能なものにする．4）図中の文字は，刷り上がりで本文とほぼ同じ10－13級（7－9ポイント），線の太さは0．15－0．3mmになるよう原図を作成する．5）図や表はA4縦で作成する．一ページに一つの図あるいは表とする．図のタイトルや表の説明（Figure legends）は図の印刷を希望する位置に記載する．図と表の挿入箇所は投稿論文中の右余白に示すこと．6）組図の原稿は，貼込み間隔や角度を正確にする．7）写真は，Ａ4判の用紙に貼り，必要な文字，記号などを記入する．写真の拡大率は，単位長さのバーで表す．8）患者の顔や特徴ある身体の一部の写真を使用する場合は，目隠し等により個人が特定できないように配慮するとともに，患者本人あるいは後見人から文書により許可を得ること．9）記号は中心の明確な○●□■◇◆などを使用する．10）記号を使用する場合の凡例は，脚注に置かずに図中に入れる．7．表1）罫線はできる限り入れない．2）標準偏差は，（ ）もしくは±とし，信頼区間との混同を避けるために説明を入れる．3）表題が英文字の場合は書き出しのみを大文字にし，それ以後は小文字とする．しかし略号はこの限りではない．4）単位などの表記は同一言語に統一する．単位（unit），平均（mean），標準偏差（SD）94（94）第３５巻１号　　　４Ｃ１５０　１Ｃ１３３／本文　※３１‐１から組体裁変更　ＯＴＦ／０９３～０９５　投稿の手引き  2016.07.13 11.45.34  Page 94 Table1 Mechanical properties of specimenSpecimenTensilestrength MpaElongation％A 500（20） 10．2（3．3）B 300（15） 5．4（2．3）（ ）：SD表1 試料の力学的性質試料引張強さMpa伸び％A 500±20 10．2±3．3B 300±15 5．4±2．3平均±標準偏差（例：）8．その他本規定ならびに「投稿の手引き」に規定されていない事項については，編集委員会にお尋ね下さい．投稿の手引き，投稿規定，チェックリストのファイルは，ホームページ（http : //www.hoku-iryo-u.ac.jp/~dental-society/）からダウンロード出来ます．95（95）第３５巻１号　　　４Ｃ１５０　１Ｃ１３３／本文　※３１‐１から組体裁変更　ＯＴＦ／０９３～０９５　投稿の手引き  2016.07.13 11.45.34  Page 95 

投稿の手引き

薄井 明

羽化したばかりのゴフマン社会学―第二公刊論文（1952）に関する一考察―

北海道医療大学学術リポジトリCONTENTS著者 北海道医療大学歯学会journal orpublication title北海道医療大学歯学雑誌 volume 35number 2year 2016-12-31URL http://id.nii.ac.jp/1145/00064430/The Dental Journal of Health Sciences University of HokkaidoVOL．35，NO．2，DECEMBER，2016CONTENTSORIGINAL1 Influence of type 2 diabetes on parasympathetic vasodilation in salivary glands in rats.Toshiya SATO, Hisayoshi ISHII …………………………………………………………………………………（97）13 Development of a method for measurement of intracellular IP3 concentrations in salivary acinar cellsusing the cytosolic type IP3−biosensor “cLIBRAvIIS”.Akihiro NEZU, Takao MORITA, Akihiko TANIMURA ………………………………………………………（109）23 Lysophosphatidic acid is involved in a cellular signaling of periodontal ligament and tooth developmentToshiya ARAKAWA, Nobuko OBARA, Miki OKAYAMA, Nattakarn HOSIRILUCK，Itaru MIZOGUCHI, Kazuharu IRIE, Taishin TAKUMA ………………………………………………………（119）REVIEW33 Effects of Melatonin on Periodontitis and Alveolar Bone Loss : a ReviewNazmus SHALEHIN, Md Riasat HASAN, Kazuharu IRIE ……………………………………………………（129）CLINICAL REPORT37 A case with open bite treated by temporary anchorage device (TAD)Miki OKAYAMA, Yukie OKA, Itaru MIZOGUCHI……………………………………………………………（133）45 A case of Myoepithelioma of the palateTetsuro MORIKAWA, Kosuke TAKIMOTO, Bhoj Raj ADHIKARI, Puja NEOPANE,Fumiya HARADA, Masafumi UTSUNOMIYA, Takatoshi CHUJO, Koki YOSHIDA, Jun SATO,Michiko NISHIMURA, Hiroki NAGAYASU and Yoshihiro ABIKO …………………………………………（141）DENTAL INFORMATION51 Recent topics …………………………………………………………………………………………………………（147）】Ｓｅｒｖｅｒ／歯学雑誌／第３５巻２号　　　４Ｃ１５０　１Ｃ１３３／目次　ＯＴＦ／目次（英文）  2017.01.17 17.39.56  Page 2 

北海道医療大学学術リポジトリ目次著者 北海道医療大学歯学会雑誌名 北海道医療大学歯学会雑誌巻 38号 1発行年 2019-06-30URL http://id.nii.ac.jp/1145/00064735/北海道医療大学歯学雑誌第38巻 第1号 令和元年6月目 次〔原著〕1 歯原性上皮細胞のマイグレーションにおける成長因子とケモカインの相互作用村田 佳織，石田 成美，齊藤 正人，谷村 明彦…………………………………………………………（1）9 関節荷重が成長期ラット下顎頭軟骨の細胞外基質mRNA発現に及ぼす影響山口 優，樫尾 治奈，中尾 友也，飯嶋 雅弘……………………………………………………………（9）21 タモギタケ由来エルゴチオネインの短期及び長期作用による歯肉上皮細胞における遺伝子発現変化のバイオインフォマティクス解析佐藤 惇，森川 哲郎，Durga PAUDEL，Puja NEOPANE，平木 大地，大西 綾，宇津宮 雅史，高井 理衣，植原 治，吉田 光希，安彦 善裕……………………………………………………………（21）〔症例報告〕29 A Rare Case of Peripheral Odontogenic Keratocyst in the Buccal Space : A Case ReportBhoj Raj ADHIKARI, Durga PAUDEL, Fumiya HARADA, Tetsuro MORIKAWA,Eiji NAKAYAMA, Tsuyoshi SHIMO, Hiroki NAGAYASU, Yoshihiro ABIKO ………………………………（29）〔歯学情報〕37 最近のトピックス………………………………………………………………………………………………………（37）41 北海道医療大学歯学会第37回学術大会 定例講演会・一般講演抄録……………………………………………（41）66 会務報告…………………………………………………………………………………………………………………（66）71 北海道医療大学歯学会会則……………………………………………………………………………………………（71）73 北海道医療大学歯学雑誌 投稿規程…………………………………………………………………………………（73）78 編集後記…………………………………………………………………………………………………………………（78）】Ｓｅｒｖｅｒ／歯学雑誌／第３８巻１号　　　４Ｃ１５０　１Ｃ１３３／目次　ＯＴＦ／目次（和文）  2019.06.27 17.57.45  Page 1 

田隈 泰信

SNAREへの道

村田 佳織

石田 成美

齊藤 正人

谷村 明彦

北海道医療大学学術リポジトリ歯原性上皮細胞のマイグレーションにおける成長因子とケモカインの相互作用著者 村田 佳織, 石田 成美, 齊藤 正人, 谷村 明彦雑誌名 北海道医療大学歯学会雑誌巻 38号 1ページ 1-7発行年 2019-06-30URL http://id.nii.ac.jp/1145/00064738/? ???????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????Horwitz & Webb, 2003?????????????????????????????????????????????????????????????????????????????????????????????????????????? ??????? ??????? ??????? ????????????????????????????????????????????????????????? ?? ?????? ?????? ??? ????????? ?? ??? ????????? ????? ?????? ?????????? ????Kaori MURATA???Narumi ISHIDA???Masato SAITOH???Akihiko TANIMURA????Division of Pharmacology, Department of Oral Biology, School of Dentistry, Health Sciences University of Hokkaido??Division of Pediatric Dentistry, Department of Oral Growth and Development, School of Dentistry,Health Sciences University of Hokkaido??? ??????Dental epithelial cell?Migration?Epidermal Growth Factor (EGF)?C−X−C motif chemokine Ligand 12(CXCL12)????????Cell migration is a crucial cell function, and involved inmany biological processes including embryo genesis, woundhealing, and cancer metastasis. In tooth morphogenesis, theinteractions between the dental epithelium and mesenchymeare important for the proliferation and differentiation of thecells involved, and contribute to form the right number andshape of teeth. In these processes, cell migration plays animportant role.The SF2 cell is a preameloblast cell line originating fromdental epithelium, and this study examined the factors con-trolling migration of SF2 cells. And, we identify factorscontrolling the migration of SF2 cells.Observation of DIOC6−stained SF2 cells using confocallaser scanning microscopy suggested that the migration ofSF2 cells are controlled by factors present in the serum. Fora more quantitative analysis, we examined the effect of se-rum on the cell migration using the OrisTM cell migration as-say kit, and confirmed that the cell migration of SF2 cellsincreased in the presence of serum. This serum−dependentcell migration was inhibited by gefitinib, an epidermalgrowth factor (EGF) inhibitor, indicating some role of EGFin the migration. Next we examined the effects of EGF andthe C−X−C motif chemokine ligand 12 (CXCL12) on themigration in serum − free conditions. Neither EGF norCXCL12 promoted cell migration. However, addition ofEGF and CXCL12 promoted the cell migration to an extentcomparable to that with serum present. It has been knownthat EGF and CXCL 12 respectively activate a tyrosinekinase receptor EGF receptor and the Gi protein−coupledreceptor CXCR4. The results here suggest that simultaneousactivation of these two pathways is necessary for the migra-tion of SF2 cells. Addition of EGF and CXCL12 in thepresence of serum did not increase cell migration further,suggesting that the factors, including the cytokine andchemokines in serum are sufficient for cell migration.??????????? ??????????? ???????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????Thesleff,2003 ; Nakamura et al., 2008??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????AMBN???????????????????????????????????????????He et al., 2010 ; Nakamura et al., 2017????????????????????????????????????????Nakamura et al., 2017 ; Liuet al., 2018????????????????????????????????????????????????????????????????????????????????????????SF2?????????????????????????????????????AMBN?????SF2????????iPS????????????????????????????????????????????????????????????????Arakaki et al., 2012???????SF2?????????????????????????????????????????????????????????????????Qiang et al., 2004 ; Agle et al.,2011 ; Maretzky et al., 2011 ; Sokol & Luster, 2015???????????????????EGF?????????????SAS???????????????????????????Ohnishi et al., 2017????????????????????????????????CXC chemokine ligand 12?CXCL12??CXC chemokinereceptor 4?CXCR4????????????????Busillo & Benovic, 2007????????????????mHAT9???CXCL12?????????????????????????Yokohama − Tamaki et al. ,2015??CXCR4?CXCL12???????????????????????????????????????????????????????????????????EGF?CXCL12?????SF2??????????????????????????????????????SF2???????????????????????????????????????? fetal bovine serum?FBS, HyClone, Buckinghamshire, England???Dul-becco’s Modified Eagle Medium?Nutrient Mixture F−12Ham?DMEM/F12, Gibco, MA. U.S.A.???????????????Epidermal growth factor?EGF, R&D SYS-TEMS, MN, U.S.A??Stromal Cell−Derived Factor−1 α,Human, recombinant?CXCL12, Wako, Osaka, Japan??EGF???????????Gefitinib, AOBIOUS, MA,U.S.A?????????????????3,3’−Dihexy-loxacarbocyanine Iodide?DIOC6, Enzo Life Sciences, NY,U.S.A???????????????????????????????????????????????NA=1.30, Nikon, Tokyo, Japan??????????????????????Nikon, C1????????nm???????????????nm?????????????????nm??????DIOC6????????????????????????mm?Glass base dish?Iwaki, Shizuoka, Japan??????SF2??????FBS???????????DMEM/F12?????????????????μM DIOC6?????????????????????????????????????????????????????????????????????????TOKAI HIT, Shizuoka,Japan??????????CO??????????????????DIOC6????SF2???????????????????????????????????????????????????????μM DIOC6????????????????????????????????????????????????????Aqua−Poly/Mount?Polyscience, PA, U.S.A???????????????????????????????????????NIS−Elements?Nikon??????????? ?? ?? ???????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????SF2???96 well plate?FALCON, NY, U.S.A???????cells/mL??????????????????????????????????????????????????Gefitinib??μM??μM??????????????????????????????????????????????CyQUANT Cell ProliferationAssay Kit?invitorogen????????????????????SF2????????cells/mL???well plate?OrisTM CellMigration Assay kit, PLATYPUS TECHNOLOGIES, WI.U.S.A???????????????????DMEM/F12?????????????????????????????????????????Gefitinib?EGF?CXCL12????????????????????????????????????????????????????????????????????????????????????????????????????????????????Sceheffe????Fisher?????????????????Kruskal Wal-lis???????????????p?????????????????????????????????? ???SF2???????????DIOC6?????SF2???????????????????????????????????SF2?????????????????????????????????????A?????????????????????????????????B????????????????????????????????????????C?????OrisTM Cell Migration Assay kit??????????????????????????????????????????????????????????????96 well plate????????????????????????????????????????????????????????????????A????????????B−D?????????????????????????????????????????????????????????????????????????????????????????????????????????B???????????????????? ???C????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? Migration assay????OrisTM Cell Migration Assay Kit?????????????????????????The Dental Journal of Health Sciences University of Hokkaido 38 2019??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????EGF??????????????????????????????????????????????????????????SF2???????????????EGF???????????EGF??????????Gefitinib?????????Gefitinib??μM??????????????????????????????????????D?????????μM Gefitinib????????????????data not shown?????????????????Gefitinib?????????????????????????????????Gefitinib??????????????????????????????????????????????????EGF??????????????????Gefitinib???????SF2?????????????????????EGF????????????????????????????????????????????EGF????????????????????????????????EGF?????????????????????????????????? ????????????SF2??????A?SF2???2μM DIOC6????????????????????????????????????????????????????????????B, C???????B???????????C??????DIOC6????????????SF2??????????????????????????????????C???????????????????????????? SF2????????????????SF2????????????OrisTM cell migration assay kit???????????????????pre−migration????????post−migration????????????????????A????????????????B???????????????C?????Gefitinib??μM????????????D????????????????????????????? Kaori MURATA et al.?Effects of growth factor and chemokine on the migration of the dental epithelial cell????????????????????????????????????????????????????????????????????????????????????????????????????CXCL12???????????????????????CXCL12??????????????????????????????????????CXCL12??????????????????????????????????????EGF?CXCL12??????????????????????????????????????????????EGF?CXCL12?EGF?CXCL12????????????????????????????????????? ??????????????????????????? SF2?????????????Gefitinib????????????????????????????????????????????????????????μM?????μM?Gefitinib?????????????????????????????????????????Gefit-inib?????????????????????????? SF2??????????????????Gefitinib???????????????????????????Gefitinib??????????SF2????????????????????Area closure???????????????????????????????????p???????p?????? SF2???????????????EGF?CXCL12????????????????EGF???CXCL12???EGF?CXCL12?????????????????????????EGF???CXCL12?????????????????????????????????????p???????????????? ?? ???????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????OrisTM Cell Migration Assay kit????????????????????????SF2??????????????????????????????????????????????????????SF2????????????EGF??????????Gefitinib?????????????EGF?????????????EGF?????????????????????????EGF?????????????????????????????????????CXCL12?EGF?????????????????????????????????????CXCL12?????????????????????????????EGF???????????????????????????????????????????????????????????EGF?CXCL12?????????????????????????????????????????????EGF???????????????????????????????????EGF???????????????????????EGF???????????????????EGF?????ErbB1?ErbB2?ErbB3?ErbB4????????????Wang??????????ErbB?????????????EGF?????????????????????????????????????????????????Dawson et al. , 2005 ; Zeng & Harris,2014???????????????SH2?????PTB?????????????????????????????????????????????????????????Ras−MAP???????PI3?????Akt??????????C?PLC??Ca????????????C?PKC???????Wells,1999 ; Zeng & Harris, 2014????????????SAS????????????EGF????????Akt?????????????????????????????????Ohnishi et al., 2017??????????????????EGF???????????????????????????HSC4???????????EGF????????????????????????????Ohnishi et al., 2017??CXCL12?Stromal cell−derived factor1α?SDF−1α????????????????G????????????GPCR????CXCR4??????CXCR4????????????????????????????????Busillo & Benovic, 2007???????????????????????????????apicalbud????????????????????????????????????????????CXCR4?apical bud???????????????????????????????Yokohama−Tamaki et al., 2015??????????????????????????????????????Zou et al., 1998??????????CXCR4????????????????????????????????????????????????Lu et al., 2001??CXCR4?Gi??GPCR????G??????α??????????????????????????cAMP??????????α??????????Src?Ras??????ERK1/2????????????????????????Busillo et al., 2010????G??????βγ???????PLC?PI3??????????????????Busillo & Benovic, 2007???????EGF???CXCL12??????Ras−MAP???????Pl3???????PLC−Ca???PKC????????????????????????????????????????????SF2????????????EGF?CXCL12??????????????????????????????CXCL12?G???????????????????????EGF???????????????????????????????????????????????????????????SF2???EGF????????????????CXCR4?????????????????????????????????????????????? ?EGF?CXCL12????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????G????????????????????????????????????????? ?????????????????????????? ?? ?? ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? ?Agle, K.A., Vongsa, R.A., & Dwinell, M.B. Chemokinestimulation promotes enterocyte migration through laminin− specific integrins. Am J Physiol Gastrointest LiverPhysiol 301 : R968−R980, 2011.Arakaki, M., Ishikawa, M., Nakamura, T. , Iwamoto, T. ,Yamada, A., Fukumoto, E., Saito, M., Otsu, K., Harada,H., Yamada, Y., & Fukumoto, S. Role of epithelial−stemcell interactions during dental cell differentiation. J BiolChem 287 : R10590−R10601, 2012.Busillo, J.M., Armando, S., Sengupta, R., Meucci, O., Bou-vier, M., & Benovic, J.L. Site−specific Phosphorylation ofCXCR4 Is Dynamically Regulated by Multiple Kinasesand Results in Differential Modulation of CXCR4 Signal-ing. J Biol Chem 285 : R7805−R7817, 2010.Busillo, J.M., & Benovic, J.L. Regulation of CXCR4 sig-naling. Biochim Biophys Acta Biomembr 1768 : R952−R963, 2007.Dawson, J.P., Berger, M.B., Lin, C.−C., Schlessinger, J.,Lemmon, M.A. , & Ferguson, K.M. Epidermal GrowthFactor Receptor Dimerization and Activation RequireLigand−Induced Conformational Changes in the DimerInterface. 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Nature393 : R595−R599, 1998.?The Dental Journal of Health Sciences University of Hokkaido 38 2019??????????????????????????????????????????????????????????????????????????????????????????????

歯原性上皮細胞のマイグレーションにおける成長因子とケモカインの相互作用

峯岸 高裕

北海道医療大学学術リポジトリ女性訪問介護職員の抑うつ症状とその関連要因ー正規・非正規職員別の検討ー著者 峯岸 高裕雑誌名 北海道医療大学看護福祉学部学会誌巻 11号 1ページ 37-42発行年 2015-03-31URL http://id.nii.ac.jp/1145/00010306/????????????????????????????????????? ????????? ???????????????????????????????????????????????????????????????????? ????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? CESD?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? ?????????? ?????????????????????????????????????????????????????????????? ??????????? ????????????????????????????????????????? ???? ?? ?????? ?? ????????????????????????????????????????????????????????????????????????????????????????????????????The Center forEpidemiologic Studies Depression scale??? CESD?????????????????????????????????????????????????????????????????????????????????????????????????MicrosoftOffice Excel??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????CESD???????????????????????????????????????????????????????????????????????????CESD????????????????????????????????????????????????????????????????????????????????????? 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象牙質再生におけるC C N 2 のインビトロ研究

Properties of an experimental prosthetic resin composite containing finely powdered enamel

裏表紙

投稿の手引き

羽化したばかりのゴフマン社会学―第二公刊論文（1952）に関する一考察―

CONTENTS

目次

SNAREへの道

歯原性上皮細胞のマイグレーションにおける成長因子とケモカインの相互作用

女性訪問介護職員の抑うつ症状とその関連要因ー正規・非正規職員別の検討ー

4,120

4,129