Osteosarcoma in the Skull of a Holstein Heifer

A-2-years-old heifer showed a mass on the frontal part of the skull. Cytological analysis displayed pleomorphic, polyedric and fusiform cells with extracellular hyaline material. Histologically, the excised mass showed proliferation of spindle-to-polyhedral cells that exhibited anisocytosis and anisocaryosis. Osteoid substance was demonstrated by Masson´s thricromic stain. Tumoral cells and osteoid expressed osteocalcin. This case report communicates a very rare osteosarcoma in the corneal process of a young heifer. As the heifer was previously dehorned, it is possible that this procedure was a predisposing factor for malignant growth.Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Investigación Animal del Chaco Semiárido; ArgentinaFil: Guidi, Maria Guadalupe. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; ArgentinaFil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología General Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentin

Suivi de l’évolution thermique de la Méditerranée

Le but de ce travail de Bachelor est d’automatiser la récolte des données de relevé thermiques de la Méditerranée ainsi que leur stockage dans une base de données, et de fournir un moyen simple de visualisation de ces informations. ETAT DE L’ART Avant de débuter le développement du projet, il est nécessaire de rechercher, lister et comparer les différents outils déjà disponibles sur le marché. Chaque outil se différencie par sa palette de fonctionnalités, sa simplicité, son utilité, sa performance ou même son coût. Il est donc essentiel de passer par cette étape de recherche afin d’avoir une vue d’ensemble et de choisir les outils les mieux adaptés pour le projet. PROJET Le projet final est destiné à être installé au sein du CREALP, qui l’utilisera aux côtés de ses autres outils de surveillance. Les données seront : Téléchargées régulièrement Converties Insérées dans une base de données Affichées sur une page web interne La plupart des technologies utilisées dans ce projet sont imposées par le CREALP afin de correspondre au mieux à leur système et méthodes de travail. RAPPORT Le présent rapport détaille les recherches, les grandes étapes du projet ainsi que les fonctionnalités. Un CD-ROM est joint à ce rapport, contenant entre-autres, le code source ainsi que les outils utilisés

Power Calculations for Replication Studies

The reproducibility crisis has led to an increasing number of replication studies being conducted. Sample sizes for replication studies are often calculated using conditional power based on the effect estimate from the original study. However, this approach is not well suited as it ignores the uncertainty of the original result. Bayesian methods are used in clinical trials to incorporate prior information into power calculations. We propose to adapt this methodology to the replication framework and promote the use of predictive instead of conditional power in the design of replication studies. Moreover, we describe how extensions of the methodology to sequential clinical trials can be tailored to replication studies. Conditional and predictive power calculated at an interim analysis are compared and we argue that predictive power is a useful tool to decide whether to stop a replication study prematurely. A recent project on the replicability of social sciences is used to illustrate the properties of the different methods.Comment: 28 pages, 4 figures (+ 1 in Appendix), 3 tables (+2 in Appendix

The assessment of replication success based on relative effect size

Replication studies are increasingly conducted in order to confirm original findings. However, there is no established standard how to assess replication success, and, in practice, many different approaches are used. The purpose of this paper is to refine and extend a recently proposed reverse-Bayes approach for the analysis of replication studies. We show how this method is directly related to the relative effect size, the ratio of the replication to the original effect estimate. This perspective leads to a new proposal to recalibrate the assessment of replication success, the golden level. The recalibration ensures that, for borderline significant original studies, replication success can only be achieved if the replication effect estimate is larger than the original one. Conditional power for replication success can then take any desired value if the original study is significant and the replication sample size is large enough. Compared to the standard approach to require statistical significance of both the original and replication study, replication success at the golden level offers uniform gains in project power and controls the type-I error rate if the replication sample size is not smaller than the original one. An application to data from four large replication projects shows that the new approach leads to more appropriate inferences, as it penalizes shrinkage of the replication estimate, compared to the original one, while ensuring that both effect estimates are sufficiently convincing on their own

Programming language abstractions for mobile code

Scala is a general-purpose programming language developed at EPFL. It combines the most important concepts found in object-oriented and functional languages. Scala is a statically typed language; in particular it features an advanced type system and supports local type inference. Furthermore it integrates well with the Java and .net platforms: their libraries are accessible without glue code and the Scala compiler generates code for both execution environments. The Scala programming language has several features that make it desirable as a language for distributed application programming. In particular, it supports first-class functions which are useful in relation with the notions of distributed scope and code mobility. In that context, the missing support for run-time types is one important drawback of the Java run-time environment as a target platform. This thesis focuses on the realisation of a new concept combining essential notions from the functional and distributed programming and implying the extension of the notion of lexical scoping to the distributed context. In short, we claim that the notion of lambda abstraction provides an elegant way for dealing with the dynamic rebinding of local references in a distributed execution environment. The key ideas exposed in this research work have been implemented in our Scala compiler. This helped us to evaluate the used techniques, in particular their impact on the reliability and the performance of distributed programs. So far, most research works related to the present subject have focused on functional programming languages, in particular on the ML language family

Nematode infection on beef cattle in two different productive regions of Argentina's Northwest

Cattle production in northwest Argentina (NOA) in the last 10 years has grown in importance. However, this growth of livestock brought productive and health constraints such as infection with gastrointestinal nematodes (GIN). Nematode infection may reduce productivity of grazing cattle [1-5]. Free-living forms of GIN exhibit a close relationship with climatic factors, the environment and animal management, affecting GIN development, survival and final infestation rate of pastures [1-7].Inst. Investigación Animal del Chaco SemiáridoFil: Suarez, Víctor Humberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Martinez, Gabriela Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentin

Osteosarcoma in the Skull of a Holstein Heifer

A-2-years-old heifer showed a mass on the frontal part of the skull. Cytological analysis displayed pleomorphic, polyedric and fusiform cells with extracellular hyaline material. Histologically, the excised mass showed proliferation of spindle-to-polyhedral cells that exhibited anisocytosis and anisocaryosis. Osteoid substance was demonstrated by Masson´s thricromic stain. Tumoral cells and osteoid expressed osteocalcin. This case report communicates a very rare osteosarcoma in the corneal process of a young heifer. As the heifer was previously dehorned, it is possible that this procedure was a predisposing factor for malignant growth.Facultad de Ciencias Veterinaria

Aspectos toxicocinéticos de la intoxicación con Heterophyllaea pustulata y su efecto sobre la proliferación y muerte celular en la epidermis de cabras

Heterophyllaea pustulata Hook f. (Rubiaceae) es una planta fotosensibilizante que produce pérdidas en el Noroeste Argentino. Su efecto tóxico ha sido demostrado en ratones y se identificaron nueve antraquinonas (AQs) con actividad fotodinámica. El presente trabajo de tesis pretendió establecer una correlación toxicocinética entre las principales AQs (rubiadina y soranjidiol), las lesiones histopatológicas y los patrones de proliferación y muerte celular en la epidermis de cabras intoxicadas experimentalmente. Se emplearon 10 cabras adultas que fueron divididas en dos grupos, uno control (GC) y otro tratado (GT). Los animales del GT recibieron hojas y tallos finos secos y molidos de H. pustulata a la dosis de 5 g/kg de peso. El GC, por su parte, recibió una dosis similar de heno de alfalfa molido. Se evaluó el perfil hepático, el daño dérmico, la concentración sérica y dérmica de las dos AQs en ambos grupos. Las muestras de piel y sangre se obtuvieron a diferentes tiempos durante 15 días de iniciado el experimento. Los tejidos fueron procesados por las técnicas histológicas de rutina. La detección de rubiadina y soranjidiol en muestras desuero tejido dérmico y material vegetal se determinó por cromatografía Líquida de Alta Resolución y acoplada a un espectrómetro de masas. Los patrones de proliferación celular se evaluaron mediante inmunohistoquímica con anticuerpos anti-PCNA, BAX2 y BCL2 para identificar células en mitosis y apoptosis respectivamente. Todos los animales del GT expresaron signos clínicos entre las 8-10 h post administración de la planta. Los signos fueron los característicos de un cuadro de fotosensibilización. En cuanto al perfil hepática solo la glutamato-oxalacetato transaminasa (GOT), la bilirrubina directa (BD) y bilirrubina total (BT)) arrojaron modificaciones durante los primeros 5 días. En concordancia, las AQs (soranjidiol y rubiadina) fueron detectadas en suero de todos los animales del GT a partir de las 8 h post administración, siendo sus niveles máximos entre las 24 y hasta las 72 h para luego descender. En piel no se observaron niveles detectables de ninguna de las AQs bajo estudio. En los estudios histopatológicos, inicialmente, se observaron cambios degenerativos, edema e inflamación. El día 8, la necrosis epidérmica fue intensa y comenzaron los fenómenos de reparación, que incluyeron la proliferación vascular y conjuntiva con hiperplasia epidérmica. La expresión de los antígenos BCL2 y BAX fue máxima entre las 32 y 72 h, mientras que el PCNA aumentó su expresión entre los 8 y 15 días. Los resultados de este trabajo demuestran que fotosensibilización puede considerarse como una forma acelerada de daño directo de la piel y que rubiadina y soranjidiol son determinantes en la toxicidad de H. pustulata.Facultad de Ciencias Veterinaria