Ammonia volatilization from irrigated and non-irrigated winter wheat plots in the North China Plain - Quantification and modeling

China’s growing population led to a drastic intensification of agriculture and livestock production in the last 50 years. Excessive mineral nitrogen (N) fertilizer application and intensive livestock production cause high N losses to the environment. Pathways of N losses may include gaseous N emissions via nitrification/denitrification (N2O, N2), ammonia (NH3) volatilization, nitrate leaching and surface run-off from soils. Ammonia emissions are one of the most important N loss pathways in the North China Plain (NCP) contributing to soil acidification, eutrophication of ecosystems and causing human health problems through combining with particles in the atmosphere which also impair visibility. For developing mitigation measures in a winter wheat cropping system, systematic measurements of NH3 volatilization were conducted in the NCP in Zhengding, 260 km southwest of Beijing. Ammonia emissions were measured with the calibrated Dräger-Tube method during the main crop growing season of winter wheat from April to June 2016. The treatments included urea and urea followed by immediate irrigation. Additionally, soil samples were taken from three depth increments (0-30, 30-60 and 60-90 cm) before and after fertilization and the NH3 volatilization was simulated with the HERMES model. The soils showed highest mineral nitrogen (Nmin) contents of up to 340 kg ha-1 (0-90 cm) after fertilization. A decrease in the calcium carbonate content and soil pH in topsoils (0-20 cm) (pH: 6.7) compared to subsoil horizons (pH: 7.7) was attributed to the long-term application of ammonium-based fertilizers as well as to high atmospheric deposition rates of ammonium and sulfuric compounds. Urea applied to winter wheat showed an NH3 loss equal to 22% the of applied N. Application of urea to winter wheat followed by irrigation yielded a reduction of the NH3 volatilization to 0.1% of the applied N. An improved N management based on the soil Nmin content is recommended to improve nitrogen use efficiency and to reduce N losses to the environment. Irrigation after fertilization can be recommended for reduction of NH3 volatilization, provided that other N loss pathways are of minor importance. The NH3 volatilization sub-module of the HERMES model enabled to simulate ammonia volatilization in the NCP satisfactorily. It is suggested to validate the model with further data sets from the NCP or from regions with comparable conditions

Early stage transplantation of bone marrow cells markedly ameliorates copper metabolism and restores liver function in a mouse model of Wilson disease

<p>Abstract</p> <p>Background</p> <p>Recent studies have demonstrated that normal bone marrow (BM) cells transplantation can correct liver injury in a mouse model of Wilson disease (WD). However, it still remains unknown when BM cells transplantation should be administered. The aim of this study was to investigate the potential impact of normal BM cells transplantation at different stages of WD to correct liver injury in toxic milk (tx) mice.</p> <p>Methods</p> <p>Recipient tx mice were sublethally irradiated (5 Gy) prior to transplantation. The congenic wild-type (DL) BM cells labeled with CM-DiI were transplanted via caudal vein injection into tx mice at the early (2 months of age) or late stage (5 months of age) of WD. The same volume of saline or tx BM cells were injected as controls. The DL donor cell population, copper concentration, serum ceruloplasmin oxidase activity and aspartate aminotransferase (AST) levels in the various groups were evaluated at 1, 4, 8 and 12 weeks post-transplant, respectively.</p> <p>Results</p> <p>The DL BM cells population was observed from 1 to 12 weeks and peaked by the 4^thweek in the recipient liver after transplantation. DL BM cells transplantation during the early stage significantly corrected copper accumulation, AST across the observed time points and serum ceruloplasmin oxidase activity through 8 to 12 weeks in tx mice compared with those treated with saline or tx BM cells (all <it>P </it>< 0.05). In contrast, BM cells transplantation during the late stage only corrected AST levels from 4 to 12 weeks post-transplant and copper accumulation at 12 weeks post-transplant (all <it>P </it>< 0.05). No significant difference was found between the saline and tx BM cells transplantation groups across the observed time points (<it>P </it>> 0.05).</p> <p>Conclusions</p> <p>Early stage transplantation of normal BM cells is better than late stage transplantation in correcting liver function and copper metabolism in a mouse model of WD.</p

X-Ray Fluorescence Microscopy Reveals Accumulation and Secretion of Discrete Intracellular Zinc Pools in the Lactating Mouse Mammary Gland

The mammary gland is responsible for the transfer of a tremendous amount of zinc ( approximately 1-3 mg zinc/day) from maternal circulation into milk during lactation to support the growth and development of the offspring. When this process is compromised, severe zinc deficiency compromises neuronal development and immune function and increases infant morbidity and/or mortality. It remains unclear as to how the lactating mammary gland dynamically integrates zinc import from maternal circulation with the enormous amount of zinc that is secreted into milk.Herein we utilized X-ray fluorescence microscopy (XFM) which allowed for the visualization and quantification of the process of zinc transfer through the mammary gland of the lactating mouse. Our data illustrate that a large amount of zinc first accumulates in the mammary gland during lactation. Interestingly, this zinc is not cytosolic, but accumulated in large, discrete sub-cellular compartments. These zinc pools were then redistributed to small intracellular vesicles destined for secretion in a prolactin-responsive manner. Confocal microscopy identified mitochondria and the Golgi apparatus as the sub-cellular compartments which accumulate zinc; however, zinc pools in the Golgi apparatus, but not mitochondria are redistributed to vesicles destined for secretion during lactation.Our data directly implicate the Golgi apparatus in providing a large, mobilizable zinc storage pool to assist in providing for the tremendous amount of zinc that is secreted into milk. Interestingly, our study also provides compelling evidence that mitochondrial zinc pools expand in the mammary gland during lactation which we speculate may play a role in regulating mammary gland function

Impact of clinical phenotypes on management and outcomes in European atrial fibrillation patients: a report from the ESC-EHRA EURObservational Research Programme in AF (EORP-AF) General Long-Term Registry

Background: Epidemiological studies in atrial fibrillation (AF) illustrate that clinical complexity increase the risk of major adverse outcomes. We aimed to describe European AF patients\u2019 clinical phenotypes and analyse the differential clinical course. Methods: We performed a hierarchical cluster analysis based on Ward\u2019s Method and Squared Euclidean Distance using 22 clinical binary variables, identifying the optimal number of clusters. We investigated differences in clinical management, use of healthcare resources and outcomes in a cohort of European AF patients from a Europe-wide observational registry. Results: A total of 9363 were available for this analysis. We identified three clusters: Cluster 1 (n = 3634; 38.8%) characterized by older patients and prevalent non-cardiac comorbidities; Cluster 2 (n = 2774; 29.6%) characterized by younger patients with low prevalence of comorbidities; Cluster 3 (n = 2955;31.6%) characterized by patients\u2019 prevalent cardiovascular risk factors/comorbidities. Over a mean follow-up of 22.5 months, Cluster 3 had the highest rate of cardiovascular events, all-cause death, and the composite outcome (combining the previous two) compared to Cluster 1 and Cluster 2 (all P &lt;.001). An adjusted Cox regression showed that compared to Cluster 2, Cluster 3 (hazard ratio (HR) 2.87, 95% confidence interval (CI) 2.27\u20133.62; HR 3.42, 95%CI 2.72\u20134.31; HR 2.79, 95%CI 2.32\u20133.35), and Cluster 1 (HR 1.88, 95%CI 1.48\u20132.38; HR 2.50, 95%CI 1.98\u20133.15; HR 2.09, 95%CI 1.74\u20132.51) reported a higher risk for the three outcomes respectively. Conclusions: In European AF patients, three main clusters were identified, differentiated by differential presence of comorbidities. Both non-cardiac and cardiac comorbidities clusters were found to be associated with an increased risk of major adverse outcomes

Clinical complexity and impact of the ABC (Atrial fibrillation Better Care) pathway in patients with atrial fibrillation: a report from the ESC-EHRA EURObservational Research Programme in AF General Long-Term Registry

Background: Clinical complexity is increasingly prevalent among patients with atrial fibrillation (AF). The ‘Atrial fibrillation Better Care’ (ABC) pathway approach has been proposed to streamline a more holistic and integrated approach to AF care; however, there are limited data on its usefulness among clinically complex patients. We aim to determine the impact of ABC pathway in a contemporary cohort of clinically complex AF patients. Methods: From the ESC-EHRA EORP-AF General Long-Term Registry, we analysed clinically complex AF patients, defined as the presence of frailty, multimorbidity and/or polypharmacy. A K-medoids cluster analysis was performed to identify different groups of clinical complexity. The impact of an ABC-adherent approach on major outcomes was analysed through Cox-regression analyses and delay of event (DoE) analyses. Results: Among 9966 AF patients included, 8289 (83.1%) were clinically complex. Adherence to the ABC pathway in the clinically complex group reduced the risk of all-cause death (adjusted HR [aHR]: 0.72, 95%CI 0.58–0.91), major adverse cardiovascular events (MACEs; aHR: 0.68, 95%CI 0.52–0.87) and composite outcome (aHR: 0.70, 95%CI: 0.58–0.85). Adherence to the ABC pathway was associated with a significant reduction in the risk of death (aHR: 0.74, 95%CI 0.56–0.98) and composite outcome (aHR: 0.76, 95%CI 0.60–0.96) also in the high-complexity cluster; similar trends were observed for MACEs. In DoE analyses, an ABC-adherent approach resulted in significant gains in event-free survival for all the outcomes investigated in clinically complex patients. Based on absolute risk reduction at 1 year of follow-up, the number needed to treat for ABC pathway adherence was 24 for all-cause death, 31 for MACEs and 20 for the composite outcome. Conclusions: An ABC-adherent approach reduces the risk of major outcomes in clinically complex AF patients. Ensuring adherence to the ABC pathway is essential to improve clinical outcomes among clinically complex AF patients

Impact of renal impairment on atrial fibrillation: ESC-EHRA EORP-AF Long-Term General Registry

Background: Atrial fibrillation (AF) and renal impairment share a bidirectional relationship with important pathophysiological interactions. We evaluated the impact of renal impairment in a contemporary cohort of patients with AF. Methods: We utilised the ESC-EHRA EORP-AF Long-Term General Registry. Outcomes were analysed according to renal function by CKD-EPI equation. The primary endpoint was a composite of thromboembolism, major bleeding, acute coronary syndrome and all-cause death. Secondary endpoints were each of these separately including ischaemic stroke, haemorrhagic event, intracranial haemorrhage, cardiovascular death and hospital admission. Results: A total of 9306 patients were included. The distribution of patients with no, mild, moderate and severe renal impairment at baseline were 16.9%, 49.3%, 30% and 3.8%, respectively. AF patients with impaired renal function were older, more likely to be females, had worse cardiac imaging parameters and multiple comorbidities. Among patients with an indication for anticoagulation, prescription of these agents was reduced in those with severe renal impairment, p&nbsp;&lt;.001. Over 24&nbsp;months, impaired renal function was associated with significantly greater incidence of the primary composite outcome and all secondary outcomes. Multivariable Cox regression analysis demonstrated an inverse relationship between eGFR and the primary outcome (HR 1.07 [95% CI, 1.01–1.14] per 10&nbsp;ml/min/1.73&nbsp;m2 decrease), that was most notable in patients with eGFR &lt;30&nbsp;ml/min/1.73&nbsp;m2 (HR 2.21 [95% CI, 1.23–3.99] compared to eGFR ≥90&nbsp;ml/min/1.73&nbsp;m2). Conclusion: A significant proportion of patients with AF suffer from concomitant renal impairment which impacts their overall management. Furthermore, renal impairment is an independent predictor of major adverse events including thromboembolism, major bleeding, acute coronary syndrome and all-cause death in patients with AF

Multi-ancestry study of blood lipid levels identifies four loci interacting with physical activity

Loss of 4E-BP1 expression has been linked to cancer progression and resistance to mTOR inhibitors, but the mechanism underlying 4E-BP1 downregulation in tumors remains unclear. Here we identify Snail as a strong transcriptional repressor of 4E-BP1. We find that 4E-BP1 expression inversely correlates with Snail level in cancer cell lines and clinical specimens. Snail binds to three E-boxes present in the human 4E-BP1 promoter to repress transcription of 4E-BP1. Ectopic expression of Snail in cancer cell lines lacking Snail profoundly represses 4E-BP1 expression, promotes cap-dependent translation in polysomes, and reduces the anti-proliferative effect of mTOR kinase inhibitors. Conversely, genetic and pharmacological inhibition of Snail function restores 4E-BP1 expression and sensitizes cancer cells to mTOR kinase inhibitors by enhancing 4E-BP1-mediated translation-repressive effect on cell proliferation and tumor growth. Our study reveals a critical Snail-4E-BP1 signaling axis in tumorigenesis, and provides a rationale for targeting Snail to improve mTOR-targeted therapies

The role of rural areas in the formation and utilisation of water resources in Poland

Za obszary wiejskie przyjęto tereny znajdujące się poza granicami administracyjnymi miast. Są to obszary o bardzo zróżnicowanym stopniu zagospodarowania i zainwestowania, w których tworzą się zasoby wody. O ich wielkości decydują głównie opady atmosferyczne, natomiast jakość i rozdział na poszczególne fazy obiegu wody wynika z cech środowiska oraz ze stanu i charakteru działalności gospodarczej. Celem pracy jest przedstawienie, na tle struktury użytkowania gruntów, stanu wykorzystania wody i jej jakości oraz wskazanie roli obszarów wiejskich w ochronie zasobów wody w Polsce. Sygnałem do pracy było pojawienie się długotrwałej i głębokiej niżówki w okresie lata 2003 r. Podstawę opracowania stanowiły publikowane materiały IMGW oraz dane statystyczne GUS. Dokumentują one stan i poziom wykorzystania zasobów wody i środowiska w pierwszym roku trzeciego tysiąclecia.Rural areas occupy the majority of the area in Poland . They are the areas of a varying degree of land development and investment, where water resources of the country are formed. The volume of water resources depends mainly on precipitation while their quality and distribution into cycle phases are the resultant of environmental features and of the condition and character of the economy. The use of the environment up to now has not, for the most part, resulted in intense anthropogenic impact. The aim of the study was to present water resources, their quality and management against a background of land use structure and to indicate the significance and tasks of rural areas for environmental protection. The study was prompted by the occurrence of a prolonged and deep low-water period during the summer of 2003. It was based on materials published by IMGW and statistical data by GUS. They document the condition and degree of utilisation of water resources and the environment during the first year of the third millennium

Changes in spring discharge in Wierzchowiska in the years 1970–2012 (Western Roztocze)

W opracowaniu przedstawiono zmiany wydajności dwu zespołów źródeł w Wierzchowiskach, położonych na obszarze Roztocza Zachodniego, dających początek górnej Sannie. Podstawą opracowania były własne pomiary wydajności wykonywane od 1970 r., uzupełnione danymi publikowanymi. Średnia wydajność, obliczona z ponad 190 pomiarów wykonanych w latach 1959–2012, wynosiła w źródłach Dworskich 48,0 dm3• s–1, a w Stokach 128,7 dm3• s–1. Zmiany wydajności obu źródeł były zbliżone, z dominacją rytmu wieloletniego. Reakcja źródeł na zasilanie, podobnie jak i stanów wód podziemnych poziomu roztoczańskiego, następuje po dłuższym okresie występowania wysokich lub niskich opadów atmosferycznych. W ujęciu miesięcznym, nie stwierdzono bezpośrednich relacji między zasilaniem i wydajnością źródeł. Jest to efekt powolnego przepływu wody opadowej przez grubą strefę aeracji, w której część stropową tworzą osady lessu eolicznego.The paper presents changes in the discharge rate of two spring complexes in Wierzchowiska, Western Roztocze, which give rise to the upper Sanna River. The study was based on own measurements of discharge carried out since 1970 and complemented with published data. The mean discharge rate calculated for over 190 measurements performed in 1959–2012 was 48.0 dm3• s–1 in the Dworskie springs and 128.7 dm3• s–1 in the Stoki spring. The changes in the discharge rate of both types of springs were comparable, with the dominance of a long-term rhythm. The reaction of the springs to recharge, likewise in the case of groundwater table in Roztocze, occurs after a long-term period of high or low atmospheric precipitation levels. On a monthly basis, no direct correlations were found between recharge and discharge of the springs. This was related to the slow rate of precipitation water flow through the thick aeration zone, the top part of which is represented by aeolian loess sediments