In vitro evaluation of carrier based obturation technique: a CBCT study

AIM: The goal of the study was to compare the ability of two different carrier based obturation (CBO) techniques to reach working length and fill in three-dimensions root canal systems, by using CBCT. MATERIALS AND METHODS: Twenty-six extracted molars were scanned with CBCT and 40 curved canals were selected (between 30° and 90°) and divided in two similar groups (n=20). All canals were prepared up to size 25 taper .06 using nickel-titanium instrumentation. The canals in the Group SC were obturated using Soft-Core obturators (Kerr, Romulus, Mi, USA), while Group TH canals (n= 20) were obturated using Thermafil Endodontic Obturators (Tulsa Dental Products, Tulsa, OK, USA), strictly following manufacturers' instructions for use. The obturations were analyzed by means of CBCT to measure the distance from the apical limit of obturation to the apical foramen and the presence of voids inside root canals. RESULTS: There was no significant difference between the two groups in the mean distance of the apical extent of the obturation (t test, p>0.05). Overfilling occurred in only 3 cases (2 in Group TH and 1 in Group SC). The percentages of voids in both groups were very low with no significant difference (Z test, p>0.05). CONCLUSIONS: The two tested CBO techniques showed similar positive results in terms of performance, even if, after checking with verifiers, in most cases the size of the selected Soft-Core obturator was one size smaller than Thermafil

A modification of the leaf-nets method for sampling benthic invertebrates in spring habitats

The full ecological potential of spring habitats remains relatively unexplored mostly because of the lack of standardized sampling procedures and difficulties to collect representative biological samples, especially in small-sized springs. Recent studies on sampling methodologies in spring habitats indicated that a modified Surber net with a reduced frame area, reliably describes the structure and composition of spring communities. This method, however, is very invasive and may severely impact the spring, especially when a large number of samples is required. The paper presents a new quantitative method (leaf-nets) for sampling crenic invertebrates which combines a rather high efficiency with negligible impacts on spring habitat structure and biota. The effectiveness of the new methodology was tested in a medium-size rheocrene spring in Central Apennine, where spring assemblages were sampled in parallel with a modified Surber net and with the new method. Taxa richness and density were higher in the Surber net, while no between-method differences were recorded for the number of insect taxa and Simpson diversity. Furthermore, the overall functional organization of Surber net and leaf-nets assemblages was very similar. The new method sampled only 25% of the individuals cumulatively collected, but 75% of the total richness, with a good representation of the structure and the functional organization of spring assemblages. In comparison with the Surber net, the negligible loss of information of the new method is highly compensated by its minor invasivity, lower impacts on spring microhabitats and invertebrate populations and by its higher versatility. Leaf-nets could also be used to assess leaf-detritus breakdown in springs, thus allowing a better ecological characterization of these ecosystems. </p

Corrigendum to: Structure, functional organization and biological traits of macroinvertebrate assemblages from leaf-bags and benthic samples in a third-order stream of Central Apennines (Italy) [Ecological Indicators 46 (2014) 84–91]

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A modification of the leaf-bags method to assess spring ecosystem functioning: benthic invertebrates and leaf-litter breakdown in Vera Spring (Central Italy)

The evaluation of leaf detritus processing (decomposition and breakdown) is one of the most simple and cost-effective method to assess the functional characteristics of freshwater ecosystems. However, in comparison with other freshwater habitats, information on leaf litter breakdown in spring ecosystems is still scarce and fragmentary. In this paper, we present results of the first application of a variant of the leaf-bags method to assess structure of macroinvertebrate assemblages and leaf-litter breakdown in a Central Apennines (Italy) cold spring which was investigated from July 2016 to October 2016. Notwithstanding the stable conditions of almost all hydrological and physico-chemical parameters, we found significant temporal differences in (i) % of mass loss of poplar leaves (ii) number of Ephemeroptera, Plecoptera and Trichoptera taxa, (iii) shredder and predator densities. We demonstrate that detritus processing in cold springs may be faster than or as fast as in warmer streams/rivers. Shredders activity and biocoenotic interactions, rather than temperature and nutrients load, were the main drivers of the process. A routine application of the modified leaf-bags may contribute to expand our knowledge on detritus processing in cold springs and may help to predict impacts of climate warming on freshwater ecosystem functioning

Idiopathic Aqueductal Stenosis: Late Neurocognitive Outcome in ETV Operated Adult Patients

ObjectiveThe aim of the present study is to evaluate a neurocognitive outcome in patients affected by late-onset idiopathic aqueductal stenosis (LIAS) who underwent endoscopic third ventriculostomy (ETV). Materials and MethodsA prospective study was conducted between January 2015 and December 2017 in a series of 10 consecutive adult patients referred to the Neurosurgery Department of IRCCS Istituto delle Scienze Neurologiche di Bologna, Bologna, Italy. All the adult patients admitted with absence of CSF flow through the aqueduct in phase-contrast (PC)-MRI sequences or a turbulence void signal in T2-weighted images in midsagittal thin-slice MR sequences underwent a specific neuroradiological, neurological, and neurocognitive assessment pre- and postoperatively. ResultsAll patients affected by gait and sphincter disturbances improved after ETV. Attentive and executive functions as well as visuo-spatial memory and verbal executive functions improved in several patients. Similarly, the affective and behavioral scales improved in almost 50% of the patients. No major complications have been recorded, and no patients required a second surgery for shunt placement. ConclusionEndoscopic third ventriculostomy represents a safe and effective surgical procedure for the treatment of LIAS. In addition to neurological improvement, we demonstrated also postoperative neurocognitive improvement mainly in attentive and executive functions, visuo-spatial memory, verbal executive functions, and behavioral and affective domains

The Enigmatic Genetic Landscape of Hereditary Hearing Loss: A Multistep Diagnostic Strategy in the Italian Population

Hearing loss is the most frequent sensorineural disorder, affecting approximately 1:1000 newborns. Hereditary forms (HHL) represent 50–60% of cases, highlighting the relevance of genetic testing in deaf patients. HHL is classified as non-syndromic (NSHL—70% of cases) or syndromic (SHL—30% of cases). In this study, a multistep and integrative approach aimed at identifying the molecular cause of HHL in 102 patients, whose GJB2 analysis already showed a negative result, is described. In NSHL patients, multiplex ligation probe amplification and long-range PCR analyses of the STRC gene solved 13 cases, while whole exome sequencing (WES) identified the genetic diagnosis in 26 additional ones, with a total detection rate of 47.6%. Concerning SHL, WES detected the molecular cause in 55% of cases. Peculiar findings are represented by the identification of four subjects displaying a dual molecular diagnosis and eight affected by non-syndromic mimics, five of them presenting Usher syndrome type 2. Overall, this study provides a detailed characterisation of the genetic causes of HHL in the Italian population. Furthermore, we highlighted the frequency of Usher syndrome type 2 carriers in the Italian population to pave the way for a more effective implementation of diagnostic and follow-up strategies for this disease

Item Reduction, Psychometric and Biometric Properties of the Italian Version of the Body Perception Questionnaire-Short Form (BPQ-SF): The BPQ-22

Body awareness disorders and reactivity are mentioned across a range of clinical problems. Constitutional differences in the control of the bodily state are thought to generate a vulnerability to psychological symptoms. Autonomic nervous system dysfunctions have been associated with anxiety, depression, and post-traumatic stress. Though interoception may be a transdiagnostic mechanism promoting the improvement of clinical symptomatology, few psychometrically sound, symptom-independent, self-report measures, informed by brain-body circuits, are available for research and clinical use. We validated the Italian version of the body perception questionnaire (BPQ)-short form and found that response categories could be collapsed from five to three and that the questionnaire retained a three-factor structure with items reduced from 46 to 22 (BPQ-22). The first factor was loaded by body awareness items; the second factor comprised some items from the body awareness scale and some from the subdiaphragmatic reactivity scale (but all related to bloating and digestive issues), and the third factor by supradiaphragmatic reactivity items. The BPQ-22 had sound psychometric properties, good convergent and discriminant validity and test-retest reliability and could be used in clinical and research settings in which the body perception assessment is of interest. Psychometric findings in light of the polyvagal theory are discussed

The Tracking performance for the IDEA drift chamber

The IDEA detector concept for a future e$^{+}$e$^{-}$ collider adopts an ultra-low mass drift chamber as a central tracking system. The He-based ultra-low mass drift chamber is designed to provide efficient tracking, a high-precision momentum measurement, and excellent particle identification by exploiting the cluster counting technique. This paper describes the expected tracking performance, obtained with full and fast simulation, for track reconstruction on detailed simulated physics events. Moreover, the details of the construction parameters of the drift chamber, including the inspection of new material for the wires, new techniques for soldering the wires, the development of an improved schema for the drift cell, and the choice of a gas mixture, will be described