Helicobacter pylori

Gastric cancer is the third cause of cancer death worldwide and infection by Helicobacter pylori (H. pylori) is considered the most important risk factor, mainly by the activity of its virulence factor CagA. H. pylori/CagA-induced chronic inflammation triggers a series of gastric lesions of increased severity, starting with gastritis and ending with cancer. IL-1β has been associated with tumor development and invasiveness in different types of cancer, including gastric cancer. Currently, it is not clear if there is an association between CagA and IL-1β at a cellular level. In this study, we analyzed the effects of IL-1β and CagA on MCF-10A nontransformed cells. We found evidence that both CagA and IL-1β trigger the initiation of the epithelial-to-mesenchymal transition characterized by β-catenin nuclear translocation, increased expression of Snail1 and ZEB1, downregulation of CDH1, and morphological changes during MCF-10A acini formation. However, only CagA induced MMP9 activity and cell invasion. Our data support that IL-1β and CagA target the β-catenin pathway, with CagA leading to acquisition of a stage related to aggressive tumors

Demands of People with Disabilities and Empowerment of Resilient Strategies

The objective of the research is to identify the resilient strategies in people with disabilities of the Technical University of Manabí. The paper presents a conceptual analysis associated with disability in university students. Some points of view related to the strategies of attention to diversity are exposed. A conceptual analysis related to resilience is provided. The situation of disabled students who are enrolled in the different careers of the Technical University of Manabí is addressed, specifying the type of disability they have. An analysis of the population is made for the study and the selection of the sample. The data related to the measurement of resilience is exposed to disabled students, for which the method developed by (Saavedra & Villalta, 2008) was used, consisting of 60 items, divided into 12 specific factors of resilience. Finally, a vision related to the resilience strategy demanded by disabled students of the Technical University of Manabí is offered

5to. Congreso Internacional de Ciencia, Tecnología e Innovación para la Sociedad. Memoria académica

El V Congreso Internacional de Ciencia, Tecnología e Innovación para la Sociedad, CITIS 2019, realizado del 6 al 8 de febrero de 2019 y organizado por la Universidad Politécnica Salesiana, ofreció a la comunidad académica nacional e internacional una plataforma de comunicación unificada, dirigida a cubrir los problemas teóricos y prácticos de mayor impacto en la sociedad moderna desde la ingeniería. En esta edición, dedicada a los 25 años de vida de la UPS, los ejes temáticos estuvieron relacionados con la aplicación de la ciencia, el desarrollo tecnológico y la innovación en cinco pilares fundamentales de nuestra sociedad: la industria, la movilidad, la sostenibilidad ambiental, la información y las telecomunicaciones. El comité científico estuvo conformado formado por 48 investigadores procedentes de diez países: España, Reino Unido, Italia, Bélgica, México, Venezuela, Colombia, Brasil, Estados Unidos y Ecuador. Fueron recibidas un centenar de contribuciones, de las cuales 39 fueron aprobadas en forma de ponencias y 15 en formato poster. Estas contribuciones fueron presentadas de forma oral ante toda la comunidad académica que se dio cita en el Congreso, quienes desde el aula magna, el auditorio y la sala de usos múltiples de la Universidad Politécnica Salesiana, cumplieron respetuosamente la responsabilidad de representar a toda la sociedad en la revisión, aceptación y validación del conocimiento nuevo que fue presentado en cada exposición por los investigadores. Paralelo a las sesiones técnicas, el Congreso contó con espacios de presentación de posters científicos y cinco workshops en temáticas de vanguardia que cautivaron la atención de nuestros docentes y estudiantes. También en el marco del evento se impartieron un total de ocho conferencias magistrales en temas tan actuales como la gestión del conocimiento en la universidad-ecosistema, los retos y oportunidades de la industria 4.0, los avances de la investigación básica y aplicada en mecatrónica para el estudio de robots de nueva generación, la optimización en ingeniería con técnicas multi-objetivo, el desarrollo de las redes avanzadas en Latinoamérica y los mundos, la contaminación del aire debido al tránsito vehicular, el radón y los riesgos que representa este gas radiactivo para la salud humana, entre otros

Demands of People with Disabilities and Empowerment of Resilient Strategies

The objective of the research is to identify the resilient strategies in people with disabilities of the Technical University of Manabí. The paper presents a conceptual analysis associated with disability in university students. Some points of view related to the strategies of attention to diversity are exposed. A conceptual analysis related to resilience is provided. The situation of disabled students who are enrolled in the different careers of the Technical University of Manabí is addressed, specifying the type of disability they have. An analysis of the population is made for the study and the selection of the sample. The data related to the measurement of resilience is exposed to disabled students, for which the method developed by (Saavedra & Villalta, 2008) was used, consisting of 60 items, divided into 12 specific factors of resilience. Finally, a vision related to the resilience strategy demanded by disabled students of the Technical University of Manabí is offered

Demands of People with Disabilities and Empowerment of Resilient Strategies

The objective of the research is to identify the resilient strategies in people with disabilities of the Technical University of Manabí. The paper presents a conceptual analysis associated with disability in university students. Some points of view related to the strategies of attention to diversity are exposed. A conceptual analysis related to resilience is provided. The situation of disabled students who are enrolled in the different careers of the Technical University of Manabí is addressed, specifying the type of disability they have. An analysis of the population is made for the study and the selection of the sample. The data related to the measurement of resilience is exposed to disabled students, for which the method developed by (Saavedra & Villalta, 2008) was used, consisting of 60 items, divided into 12 specific factors of resilience. Finally, a vision related to the resilience strategy demanded by disabled students of the Technical University of Manabí is offered

TFIIIB Subunit Bdp1 Participates in RNA Polymerase III Transcription in the Protozoan Parasite Leishmania major

Leishmania major, a protozoan parasite that diverged early from the main eukaryotic lineage, exhibits unusual mechanisms of gene expression. Little is known in this organism about the transcription factors involved in the synthesis of tRNA, 5S rRNA, and snRNAs, transcribed by RNA Polymerase III (Pol III). Here we identify and characterize the TFIIIB subunit Bdp1 in L. major (LmBdp1). Bdp1 plays key roles in Pol III transcription initiation in other organisms, as it participates in Pol III recruitment and promoter opening. In silico analysis showed that LmBdp1 contains the typical extended SANT domain as well as other Bdp1 conserved regions. Nevertheless, LmBdp1 also displays distinctive features, including the presence of only one aromatic residue in the N-linker region. We were not able to produce null mutants of LmBdp1 by homologous recombination, as the obtained double replacement cell line contained an extra copy of LmBdp1, indicating that LmBdp1 is essential for the viability of L. major promastigotes. Notably, the mutant cell line showed reduced levels of the LmBdp1 protein, and its growth was significantly decreased in relation to wild-type cells. Nuclear run-on assays demonstrated that Pol III transcription was affected in the mutant cell line, and ChIP experiments showed that LmBdp1 binds to 5S rRNA, tRNA, and snRNA genes. Thus, our results indicate that LmBdp1 is an essential protein required for Pol III transcription in L. major

Clipped histone H3 is integrated into nucleosomes of DNA replication genes in the human malaria parasite Plasmodium falciparum

The fastq files supporting the results of this article are available in the EMBL‐EBI European Nucleotide Archive (ENA: PRJEB18114; Sample group: ERG011046): http://www.ebi.ac.uk/ena/data/view/PRJEB18114.International audiencePost‐translational modifications of histone H3 N‐terminal tails are key epigenetic regulators of virulence gene expression and sexual commitment in the human malaria parasite Plasmodium falciparum. Here, we identify proteolytic clipping of the N‐terminal tail of nucleosome‐associated histone H3 at amino acid position 21 as a new chromatin modification. A cathepsin C‐like proteolytic clipping activity is observed in nuclear parasite extracts. Notably, an ectopically expressed version of clipped histone H3, PfH3p‐HA, is targeted to the nucleus and integrates into mononucleosomes. Furthermore, chromatin immunoprecipitation and next‐generation sequencing analysis identified PfH3p‐HA as being highly enriched in the upstream region of six genes that play a key role in DNA replication and repair: In these genes, PfH3p‐HA demarcates a specific 1.5 kb chromatin island adjacent to the open reading frame. Our results indicate that, in P. falciparum, the process of histone clipping may precede chromatin integration hinting at preferential targeting of pre‐assembled PfH3p‐containing nucleosomes to specific genomic regions. The discovery of a protease‐directed mode of chromatin organization in P. falciparum opens up new avenues to develop new anti‐malarials

Sequential Congo Red Elimination by UASB Reactor Coupled to Electrochemical Systems

Response surface methodology was investigated to determine the operational parameters on the degradation of Congo red dye (CR) and chemical oxygen demand (COD) in two electrochemical systems evaluated individually on effluent pretreated by an up-flow anaerobic sludge blanket (UASB) reactor. The UASB reactor was fed with 100 mg L−1 of CR and was operated for 12 weeks at different hydraulic residence times (HRTs) of 12 h, 10 h, and 8 h. Once stabilized at an HRT of 8 h, the effluent was collected, homogenized, and independently treated by electrooxidation (EO) and electrocoagulation (EC) cells. On both electrochemical systems, two electrode pairs were used; solid for EC (Fe and stainless-steel) and mesh electrodes for EO (Ti/PbO2 and Ti), and the effect of intensity (A), recirculation flow rate (mL min−1), and experimental time (min) was optimized on response variables. The maximum efficiencies of sequential systems for COD degradation and CR decolorization were 92.78% and 98.43% by EC and ≥99.84% and ≥99.71% by EO, respectively. Results indicate that the coupled systems can be used in textile industry wastewater treatment for the removal of dyes and the decolorized by-products

Textos de maestría 1. Apuntes para una clínica psicoanalítica

La publicación de algunos textos de los hoy graduados de la Maestría en Psicología Clínica con mención en Psicopatología y Psicoanálisis tiene un valor de acto, en tanto, su escritura da cuenta del camino que cada uno ha hecho durante el programa y que no es sin efectos. Siendo consecuentes con la transferencia por y para el psicoanálisis, recordamos a través de estos textos la pregunta de Lacan: “Lo que enseña psicoanálisis, ¿Cómo enseñarlo?” Respondiéndolo con una frase de Marcel Czermk: “si no sabemos en qué está aferrado el psicoanálisis, es inútil soñar con una enseñanza del psicoanálisis, se hará medicina o filosofía…”, pues el psicoanálisis es la clínica, el psicoanálisis “es una práctica que se ocupa de lo que no anda bien”

Digital Transformation in Organizations

Este libro aborda aspectos fundamentales de la transformación digital en las organizaciones, y a través de estas, en la sociedad en general. Especialistas de diversos campos disciplinares y contextos, así como líderes con experiencia en la realización de este tipo de iniciativas en organizaciones de diversos tipos y tamaños, abordan los orígenes, los fundamentos y las implicaciones de esta importante transformación. La transformación digital conduce a las organizaciones a considerar la implementación de múltiples iniciativas tecnológicas, pero también, a la realización de diversas acciones en lo social, lo cultural, lo político, lo económico, lo ecológico y lo normativo, en la configuración de un proceso de adaptación a las nuevas realidades y, en no pocas ocasiones, de un proceso que responde a la necesidad de reconfigurar, reinventar y hacer avanzar los esquemas tradicionales de organizarnos socialmente, con el interés de alcanzar nuevos estándares de bienestar, desarrollo y prosperidad para la humanidad. Este tipo de transformación implica la reconfiguración del modelo de negocio y, de hecho, el surgimiento de nuevos negocios, así como el establecimiento de formas inéditas de comerciar, comunicarse y de interactuar en todas las esferas entre los diversos actores que dan forma a la sociedad. Dado que en América Latina no existen suficientes trabajos que permitan comprender los aspectos relevantes de la transformación digital en el contexto organizacional, y de la sociedad en su conjunto, este libro pretende constituirse en una obra de referencia y de consulta para aquellas personas interesadas en este tema en el contexto actual.This book addresses the fundamental aspects of digital transformation in organizations and, through them, in society in general. Specialists from various disciplinary fields and contexts, as well as leaders with experience in carrying out this type of initiative in organizations of various types and sizes, addressed the origins, foundations and implications of this important transformation. Digital transformation leads organizations to consider the implementation of multiple technological initiatives, but also to carry out various social, cultural, political, economic, ecological, and regulatory actions, in the configuration of a process of adaptation to new realities and, in many cases, a process that responds to the need to reconfigure, reinvent, and advance the traditional models of social organization, seeking to achieve new standards of welfare, development, and prosperity for humanity. This type of transformation involves the reconfiguration of the business model and, in fact, the emergence of new businesses, as well as the formulation of unprecedented ways of trading, communicating, and interacting in all spheres among various actors shaping society. Given that in Latin America there are not enough works that allow understanding the relevant aspects of digital transformation in the organizational setting—and society as a whole—this book aims to become a reference work for those interested in this topic in the current context