Ecuadorian Amazonian Fruits: A Proposal to Value Biodiversity and Promote Food Sovereignty

Fruits are characterized as a source of vitamins and minerals, recognized as an important part of a healthy eating pattern that reduces the risk of some chronic diseases. The Ecuadorian flora in the Amazon region is rich in edible fruits, but little is known about their characteristics and nutritional importance. At the same time, over the years, traditional knowledge about them has been lost. With this implication, this work pretends to describe a research proposal that values the biodiversity of edible fruits in the Ecuadorian Amazon, so that these underutilized products are conceived as more than merchandise. For this purpose, a narrative bibliographic review and a participatory observation were carried out. The research proposal included three stages i) the characterization of fruits traded in local markets; ii) an ethnobotanical study to identify the use and preference of the fruits; and iii) the analysis of bioactive compounds of at least 5 fruits prioritized according to the characterization and ethnobotanical study. The proposal stages generated research that converges the valuation of biodiversity, traditional knowledge, and nutritional potential of Ecuadorian Amazonian fruits. Keywords: edibles, characterization, bioactives, Ecuador, ethnobotany. Resumen Las frutas se caracterizan por ser una fuente de vitaminas y minerales, reconocidas como una parte importante de un patrón de alimentación saludable que reducen el riesgo de algunas enfermedades crónicas. La flora ecuatoriana en la región amazónica es rica en frutas comestibles, pero muy poco se conoce sobre sus características e importancia nutricional. A su vez, con el pasar de los años se está perdiendo el conocimiento tradicional sobre estas. Con esta implicación, este trabajo busca describir una propuesta de investigación que valore la biodiversidad de las frutas comestibles presentes en la Amazonía ecuatoriana para que estos productos infrautilizados sean concebidos como algo más que mercancías. Para esto se realizó una revisión bibliográfica narrativa y una observación participativa. La propuesta resultante incluyó tres etapas i) la caracterización de las frutas comercializadas en los mercados locales ii) un estudio etnobotánico que identifique el uso y preferencia de las frutas y iii) el análisis de compuestos bioactivos de al menos 5 frutas priorizadas de acuerdo con la caracterización y estudio etnobotánico. Las etapas de la propuesta generaron una investigación que converge la valoración de la biodiversidad, el conocimiento tradicional y el potencial nutricional de las frutas amazónicas ecuatorianas. Palabras Clave: comestibles, caracterización, bioactivos, Ecuador, etnobotánica

Combined grazing incidence RBS and TEM analysis of luminescent nano-SiGe/SiO2 multilayers.

Multilayer structures with five periods of amorphous SiGe nanoparticles/SiO2 layers with different thickness were deposited by Low Pressure Chemical Vapor Deposition and annealed to crystallize the SiGe nanoparticles. The use of grazing incidence RBS was necessary to obtain sufficient depth resolution to separate the signals arising from the individual layers only a few nm thick. The average size and areal density of the embedded SiGe nanoparticles as well as the oxide interlayer thickness were determined from the RBS spectra. Details of eventual composition changes and diffusion processes caused by the annealing processes were also studied. Transmission Electron Microscopy was used to obtain complementary information on the structural parameters of the samples in order to check the information yielded by RBS. The study revealed that annealing at 900 °C for 60 s, enough to crystallize the SiGe nanoparticles, leaves the structure unaltered if the interlayer thickness is around 15 nm or higher

Characterization And Modeling Of Antireflective Coatings Of Si O 2, Si3 N4, And Si Ox Ny Deposited By Electron Cyclotron Resonance Enhanced Plasma Chemical Vapor Deposition

In this work the optical transmission spectra of silicon oxide (Si O2), silicon nitrides (Si3 N4), silicon-rich oxynitrides (Si Ox Ny), and antireflective coatings (ARCs), deposited by the electron cyclotron resonance enhanced plasma chemical vapor deposition onto a silicon substrate at room temperature, are studied. Simulations carried out with the MATHEMATICA program, from 0 to 1000 nm thick coatings, showed maximum transmittance in the three basic colors at 620, 480, and 560 nm for the Si O2, Si3 N4, and Si Ox Ny ARCs, respectively. However, a highly significant transmittance over a broad spectral range from visible (VIS) to near the infrared region, with optical gain in the three basic colors above 20%, is observed only at thicknesses of 80, 70, and 60 nm, respectively, for Si O2, Si3 N4, and Si Ox Ny ARCs. Among the three evaluated films, the highest transmittance in the broad spectral band in the VIS range was observed for 60 nm thick Si3 N4 films. The Fourier transform infrared spectroscopy of these films reveal high structural quality and the presence of Si-O, Si-H, N-H, and Si-N bonds, independent of thickness and deposition parameters. © 2006 American Vacuum Society.242823827White, M., Lampe, D., Blaha, F., Mack, I., (1974) IEEE J. Solid-State Circuits, SC-9, p. 1Furumiya, M., Ohkubo, H., Muramatsu, Y., Kurosawa, S., Okamoto, F., Fujimoto, Y., Nakashiba, Y., (2001) IEEE Trans. Electron Devices, 48, p. 2221Popov, O.A., Waldron, H., (1989) J. Vac. Sci. Technol. A, 7, p. 914Heavens, O.S., (1991) Optical Properties of Thin Solid Films, , Dover, New YorkAroutiounian, V.M., Maroutyan, K.R., Zatikyan, A.L., Touryan, K.J., (2002) Thin Solid Films, 403, p. 517Edwards, D.F., (1985) Handbook of Optical Constants of Solids, , edited by E. D.Palik (Academic, Washington, D.CGreen, M.L., Gusev, E.P., Degraeve, R., Garfunkel, E.L., (2001) J. Appl. Phys., 90, p. 2057Alayo, M.I., Pereyra, I., Scopel, W.L., Fantini, M.C.A., (2002) Thin Solid Films, 402, p. 154Tsu, D.V., Lucovsky, G., Mantini, M.J., Chao, S.S., (1987) J. Vac. Sci. Technol. A, 5, p. 1998Lucovsky, G., Richard, P.D., Tsu, D.V., Lin, S.Y., Markunas, J., (1986) J. Vac. Sci. Technol. A, 4, p. 681Joseph, E.A., Gross, C., Liu, H.Y., Laaksonen, R.T., Celii, F.G., (2001) J. Vac. Sci. Technol. A, 19, p. 2483Wu, X., Ossadnik, Ch., Eggs, Ch., Veprek, S., Phillipp, F., (2002) J. Vac. Sci. Technol. B, 20, p. 1368Diniz, J.A., Do Couto, A.L., Danilov, I., Tatsch, P.J., Swart, J.W., (1999) Proceedings of the XIV International Conference of Microelectronics and Packaging, p. 164Tsu, D.V., Lucovsky, G., Mantini, M.J., (1986) Phys. Rev. B, 33, p. 7069Denisse, C.M.M., Troost, K.Z., Oude Elferink, J.B., Habraken, F.H.P.M., Van Der Weg, W.F., (1986) J. Appl. Phys., 60, p. 253

Effect of plant density on yield components of common bean grown under inter-Andean Mountain conditions of Ecuador

El fréjol es un cultivo importante debido a su alto valor nutritivo. En los últimos años la producción en Ecuador de este cultivo ha disminuido drásticamente debido entre otros factores a ineficientes procesos y falta de tecnificación en la producción. Entender cómo se generan y determinan los componentes del rendimiento del grano es primordial para diseñar estrategias que permitan aumentar el rendimiento del cultivo. En este estudio, se modificó la densidad de plantas con el objetivo de evaluar su impacto sobre el número de granos, peso de granos y rendimiento, bajo condiciones de campo en una región andina de Ecuador. Se realizaron dos experimentos sembrados en fechas distintas, donde se evaluaron densidades de plantas contrastantes 4 y 11 plantas m-2. El efecto de los tratamientos se estudió sobre la duración del periodo emergencia - antesis, el número de vainas llenas y vanas, el número de granos, el peso seco de 100 granos y el rendimiento de grano. Los resultados indican que la densidad de plantas tiene un efecto significativo (p<0,05) sobre el rendimiento, alcanzando un promedio de 257,15 g m-2 en alta densidad y 151,45 g m-2 en baja densidad. Los componentes principales del rendimiento mostraron una respuesta distinta a la modificación de la densidad de plantas; el número de granos presentó una fuerte variabilidad y fue positivamente asociado con el rendimiento final del grano (p<0,05), mientras que el peso del grano no fue afectado.Common bean is an important crop due to its high nutritional value. However, its production in Ecuador has decreased due to biotic and abiotic factors. Understanding the mechanisms that determine the yield components of this crop is essential to establish strategies that allow increasing the yield. In this study, the plant density was modified to evaluate its impact on yield and its two main components, the grain number and grain weight under field conditions in an Andean region of Ecuador. Two experiments planted on different dates were performed, where two planting densities 4 and 11 plants m-2 were evaluated. The effect of the treatments was studied on the length of the period, from emergence to anthesis, the number of full and empty pods, grain number, 100 grain weight and grain yield. The results indicate that the plant density has a significant effect (p<0.05) on the yield, reaching an average of 257.15 g m-2 in high density, and 151.45 g m-2 in low density. The yield main components showed a different response to plant density modification; the grain number exhibited a strong variability and was positively associated with final grain yield (p<0.05) while the grain weight was not affected.

Quinoa in Ecuador: Recent Advances under Global Expansion

Quinoa is a highly diverse crop domesticated in the Andean region of South America with broad adaptation to a wide range of marginal environments. Quinoa has garnered interest worldwide due to its nutritional and health benefits. Over the last decade, quinoa production has expanded outside of the Andean region, prompting multiple studies investigating the potential for quinoa cultivation in novel environments. Currently, quinoa is grown in countries spanning five continents, including North America, Europe, Asia, Africa, and Oceania. Here, we update the advances of quinoa research in Ecuador across different topics, including (a) current quinoa production situation with a focus on breeding progress, (b) traditional seed production, and (c) the impact of the work of the nongovernment organization “European Committee for Training and Agriculture” with quinoa farmers in Chimborazo province. Additionally, we discuss genetic diversity, primary pests and diseases, actions for adapting quinoa to tropical areas, and recent innovations in quinoa processing in Ecuador. Finally, we report a case study describing a participatory breeding project between Washington State University and the Association of Andean Seed and Nutritional Food Producers Mushuk Yuyay in the province of Cañar

Characterization of the acetohydroxyacid synthase multigene family in the tetraploide plant Chenopodium quinoa

Background: Currently, the technology called Clearfield\uae is used in the development of crops resistant to herbicides that inhibit the enzyme acetohydroxy acid synthase (AHAS, EC 2.2.1.6). AHAS is the first enzyme of the biosynthetic pathway that produces the branched-chain of the essential amino acids valine, leucine, and isoleucine. Therefore, multiple copies of the AHAS gene might be of interest for breeding programs targeting herbicide resistance. In this work, the characterization of the AHAS gene was accomplished for the Chenopodium quinoa Regalona-Baer cultivar. Cloning, sequencing, and Southern blotting were conducted to determine the number of gene copies. Results: The presence of multiple copies of the AHAS gene as has been shownpreviously in several other species is described. Six copies of the AHAS gene were confirmed with Southern blot analyses. CqHAS1 and CqAHAS2 variants showed the highest homology with AHAS mRNA sequences found in the NR Database. A third copy, CqAHAS3, shared similar fragments with both CqAHAS1 and CqAHAS2, suggesting duplication through homeologous chromosomes pairing. Conclusions: The presence of multiple copies of the gene AHAS shows that gene duplication is a common feature in polyploid species during evolution. In addition, to our knowledge, this is the first report of the interaction of sub-genomes in quinoa

Aplastic Crisis as Primary Manifestation of Systemic Lupus Erythematosus

Aplastic crisis is an unusual feature of systemic lupus erythematosus (SLE). We report the case of a 54-year-old woman presenting with both (extravascular) Coombs-positive hemolytic anemia and laboratory findings of bone marrow hyporegeneration with concomitant severe neutropenia. A bone marrow biopsy confirmed aplastic crisis. Diagnostic work-up revealed soaring titers of autoantibodies (anti-nuclear, anti-double-stranded DNA, anti-cardiolipin-IgM, and anti-beta 2-glykoprotein-IgM antibodies), indicating a connective tissue disease as the most plausible reason for bone marrow insufficiency. As the criteria for SLE were fulfilled, we initiated an immunosuppressive therapy by steroids, which led to a rapid complete hematologic and clinical remission in our patient. In this case, we could report on one of the rare cases of SLE-induced aplastic crisis showing that this condition can be entirely reversed by immunosuppressive treatment and that SLE-induced aplastic crisis yields a good prognosis. In conclusion, in a case of aplastic crisis, physicians should be aware that SLE can be a rare cause that is accessible to specific treatment

Influence of the crystallization process on the luminescence of multilayers of SiGe nanocrystals embedded in SiO2

Multilayers of SiGe nanocrystals embedded in an oxide matrix have been fabricated by low-pressure chemical vapor deposition SiO2 onto Si wafers (in a single run at 390 ◦C and 50mTorr, using GeH4, Si2 H6 and O2) followed by a rapid thermal annealing crystallize the SiGe nanoparticles. The main emission band is located at 400 nm in both cathodoluminescence and photoluminescence at 80K and also at room temperature. The annealing conditions (temperatures ranging from 700 to 1000 ◦C and for times of 30 investigated in samples with different diameter of the nanoparticles (from ≈3 to ≥5 nm) and oxide interlayer thickness (15 and establish a correlation between the crystallization of the nanoparticles, the degradation of their composition by Ge diffusion the luminescence emission band. Structures with small nanoparticles (3–4.5 nm) separated by thick oxide barriers (≈35 nm) annealed 60 s yield the maximum intensity of the luminescence. An additional treatment at 450 ◦C in forming gas for dangling-bond the intensity of the luminescence band by 25–30%

Efecto de la densidad de plantas sobre los componentes del rendimiento de fréjol cultivado en condiciones de campo en un valle interandino de Ecuador

El fréjol es un cultivo importante debido a su alto valor nutritivo. En los últimos años la producción en Ecuador de este cultivo ha disminuido drásticamente debido entre otros factores a ineficientes procesos y falta de tecnificación en la producción. Entender cómo se generan y determinan los componentes del rendimiento del grano es primordial para diseñar estrategias que permitan aumentar el rendimiento del cultivo. En este estudio, se modificó la densidad de plantas con el objetivo de evaluar su impacto sobre el número de granos, peso de granos y rendimiento, bajo condiciones de campo en una región andina de Ecuador. Se realizaron dos experimentos sembrados en fechas distintas, donde se evaluaron densidades de plantas contrastantes 4 y 11 plantas m-2. El efecto de los tratamientos se estudió sobre la duración del periodo emergencia - antesis, el número de vainas llenas y vanas, el número de granos, el peso seco de 100 granos y el rendimiento de grano. Los resultados indican que la densidad de plantas tiene un efecto significativo (p<0,05) sobre el rendimiento, alcanzando un promedio de 257,15 g m-2 en alta densidad y 151,45 g m-2 en baja densidad. Los componentes principales del rendimiento mostraron una respuesta distinta a la modificación de la densidad de plantas; el número de granos presentó una fuerte variabilidad y fue positivamente asociado con el rendimiento final del grano (p<0,05), mientras que el peso del grano no fue afectado

Primer aislamiento y caracterización de la cepa prototipo del virus SARS-CoV-2 a inicios de la pandemia de la COVID-19 en el Perú

Introduction: Currently, infections by the SARS-CoV-2 virus exceed 600 million cases in the world. Objective: Isolation and characterization of the SARS-CoV-2 virus causing COVID-19 at the beginning of the pandemic in Peru. Materials and methods: Twenty nasal and pharyngeal swab samples were isolated SARS-CoV-2 using two cell lines, Vero ATCC CCL-81 and Vero E-6; virus identification was performed by RT-PCR and the onset of cytopathic effect (CPE) was evaluated by indirect immunofluorescence and subsequent identification by genomic sequencing. One of the most widely circulating isolates was selected and named the prototype strain (PE/B.1.1/28549/2020). Then 10 successive passages were performed in Vero ATCC CCL-81 cells to assess mutation dynamics. Results: Results detected 11 virus isolates by cytopathic effect, and subsequently confirmed by RT-PCR and indirect immunofluorescence. Of these, six were sequenced and identified as the lineages B.1, B.1.1, B.1.1.1, and B.1.205 according to the Pango lineage nomenclature. The prototype strain corresponded to lineage B.1.1. The analysis of the strains from the successive passages showed mutations mainly at in the spike (S) protein of the virus without variation in the identity of the lineage. Conclusions: Four lineages were isolated in the Vero ATCC CCL-81 cell line. Subcultures in the same cell line show mutations in the spike protein indicating greater adaptability to the host cell and variation in pathogenicity in vitro, a behavior that allows it to have more survival success.Introducción: Actualmente los contagios por el virus del SARS-CoV-2 supera los 600 millones de casos en el mundo. Objetivo: Aislar y caracterizar el virus SARS-CoV-2 causante de la COVID-19 a inicios de la pandemia en el Perú. Materiales y métodos: Se realizó el aislamiento viral a partir de 20 muestras de hisopado nasal y faríngeo positivas a SARS-CoV-2 por RT-PCR. El aislamiento se realizó en las líneas celulares Vero ATCC CCL-81 y Vero E6, evaluando el efecto citopático, la presencia del virus por RT-PCR, inmunofluorescencia indirecta (IFI) y posterior identificación por secuenciación genómica. Posteriormente, uno de los aislamientos de mayor circulación fue seleccionado y denominado cepa prototipo (PE/B.1.1/28549/2020), realizándose 10 pasajes sucesivos en células Vero ATCC CCL-81 para evaluar la dinámica de mutaciones. Resultados: Se observaron 11 aislamientos de virus por efecto citopático confirmándose por RT-PCR e IFI, de los cuales 6 fueron secuenciados identificándose los linajes B.1, B.1.1, B.1.1.1 y B.1.205, según el comité Pango de los genomas. La cepa prototipo corresponde a la variante B.1.1 y el análisis de las secuencias de los pasajes sucesivos mostró mutaciones a nivel de la proteína de la espiga (S) del virus, sin variación en la identidad del linaje. Conclusiones: Se aislaron 4 linajes en la línea celular Vero ATCC CCL-81. Los subcultivos en la misma línea celular muestran mutaciones en la proteína de la espiga, lo que indica mayor adaptabilidad a la célula hospedera y variación de la patogenicidad in vitro, comportamiento que le permite tener más éxito de supervivencia