CHARACTERIZATION AND ANTIMICROBIAL SPECTRUM OF A POTENT STREPTOMYCES SP. GOS2 ISOLATED FROM WESTERN GHATS OF KARNATAKA, INDIA

Objective: Western Ghats of Karnataka, India-a biodiversity hotspot is a rich source of microorganisms with undiscovered metabolic capabilities. The upsurge of antibiotic-resistant pathogens has created a greater demand for novel antibiotics. In context to the above a potent soil Streptomyces sp. GOS2 isolated from Agumbe regions of Western Ghats, and its metabolite was assessed and characterized for its antimicrobial spectrum.Methods: The isolation was carried out by soil serial dilution plating on Starch Casein Nitrate agar media (SCN). The obtained isolate was characterized by morphological and biochemical tests. The antimicrobial activity was assessed by well in agar methods against 28 test organisms. The partial characterization of the bioactive metabolite was carried out by thin layer chromatography (TLC) and UV–Visible spectroscopy studies.Results: The isolated GOS2 was observed as a raised powdery colony with grey colored aerial mycelium and media impregnated substrate mycelium. The spore chain was rectus with smooth spore surface. The isolate was gram positive, non-acid fast, positive for catalase, hydrogen sulphide production and starch hydrolysis, negative for casein and gelatin hydrolysis. The carbohydrate fermentation studies showed acid production in dextrose and alkali production in sucrose, lactose, maltose and starch. A prominent antibacterial activity was observed with a zone of inhibition measuring 21-27 mm. The TLC showed a purple spot and UV spectroscopy revealed λ max at 233.2 and 235 nm indicating macrolide group of antibiotics.Conclusion: Western Ghats actinomycetes are a potent source of novel antibiotic molecules

CHARACTERIZATION AND ANTIMICROBIAL POTENTIAL OF MARINE ACTINOMYCETES FROM ESTUARIES OF UTTARA KANNADA DISTRICT, KARNATAKA

The oceans cover about 70% of the Earth’s surface and harbor most of the planets biodiversity. The marine environment has become a prime resource in search and discovery for novel natural products and biological diversity and marine actinomycetes turn out to be important contributors. The diversity of marine actinomycetes is enormous and has immense scope for the discovery of novel bioactive metabolites. The present study describes the antimicrobial potential of crude extracts of marine Actinomycetes species isolated from Aghanashini, Sharavathi and Kali estuaries of Uttara Kannada district, Karnataka, India. The sediment and water samples were collected from different sites of estuaries and subjected for serial dilution and plating. A total of 43 actinomycetes isolates were obtained on Starch Casein Nitrate Agar and Kusters Agar supplemented with 10% sea water. The isolates were identified as Actinomycetes by morphological and biochemical studies. The results revealed diversity of Actinomycetes with varying spore, aerial and substrate mycelium colours such as white, grey, pink, yellow and black. The colonies formed were discrete, powdery, raised and velvety colonies with brown, yellow and maroon pigmentations. The spore chain morphology studies showed different arrangements like rectus, flexibilis, retinaculum aperatum – open loops, hooks and spira – simple spirals. Primary screening for antimicrobial activity was determined by cross streak method against 16 bacterial isolates among which 07 isolates were gram positive and 09 isolates were gram negative, 02 yeasts viz., Candida albicans and Cryptococcus neoformans and a dermatophyte Trichosporon. The actinomycetes isolates from marine environments have shown to be potent in inhibiting the test bacteria and fungi. Further studies on cytotoxic potential of the actinomycetes isolates on cell lines are under progress

Peningkatan Kompetensi Guru Dalam Mengimlementasikan Kurikulum 2013 Dalam Education 4.0

Abstrak Pengabdian kepada masyarakat merupakan bagian integral tri dharma perguruan tinggi yang melibatkan dosen, mahasiswa, dan tenaga kependidikan. Pengabdian kepada masyarakat bertujuan memberikan makna kebermanfaatan keberadaan Program Magister Administrasi Pendidikan kepada sekolah Kasih Immanuel dalam meningkatkan kompetensi mengimplementasikan kurikulum 2013. Populasi seluruh guru SD, SMP, SMA Kasih Immanuel sebanyak 15 orang dan sampel penelitian seluruh guru, namun yang hadir 12 orang. Data diperoleh dengan wawancara mendalam terhadap kepala sekolah dan guru. Selain itu data diperoleh melalui instrumen pre test dan post test. Data dianalisis menggunakan statistik deskriptif. Hasil pengabdian kepada masyarakat menunjukkan: (1) Guru mengajar merangkap di SD, SMP, dan SMA, (2) Guru yang memenuhi standar kualifikasi pendidikan 50%, (3) Guru yang sudah tersertifikasi 25%, dan (4) Pemberian bimbingan teknis implementasi kurikulum 2013 sangat bermanfaat meningkatkan kompetensi pedagogik, personal, sosial dan profesional. Implikasi pengabdian kepada masyarakat guru semakin termotivasi melengkapi rencana pelaksanaan pembelajaran (RPP) dan meningkatkan profesionalitas guru.  Kata kunci: Kompetensi, implementasi, Kurikulum 2013 Abstract Community service is an integral part of the three pillars of higher education that involves lecturers, students, and education staff. Community service aims to give meaning to the usefulness of the existence of the Educational Administration, graduate Program Universitas Kristen Indonesia to the Kasih Immanuel school in improving the competence of implementing the 2013 curriculum. The population of all elementary school, junior high school students is Kasih Immanuel as many as 15 people and a sample of all teachers, but there are 12 people present. Data obtained by in-depth interviews with principals and teachers. In addition, data were obtained through pre-test and post-test instruments. Data were analyzed using descriptive statistics. The results of community service show: (1) Teachers teaching concurrently in elementary, middle and high school, (2) Teachers who meet the 50% educational qualification standard, (3) 25% certified teachers, and (4) Providing technical implementation guidance the 2013 curriculum is very useful in increasing pedagogical, personal, social and professional. The implications of service to the teacher community are increasingly motivated to complete the lesson plan and increase teacher professionalism.  Keywords: Competence, implementation, curriculu

Potassium Levels, Sources and Time of Application on Nutrient Uptake and Nutrient Use Efficiency of Onion (Allium cepa L.)

The present study on potassium levels, sources and time of application on nutrient uptake and nutrient use efficiency of onion var. ArkaKalyan was carried out at the College of Horticulture, Bagalkot, Karnataka during Kharif season of 2015 and 2016. The uptake of nitrogen, phosphorus, potassium and zinc recorded significantly in 200 per cent RDK (203.62, 47.33, 236.36 kg ha-1 and 347.19 g ha-1 , respectively) over 100 per cent RDK. Among the potassium sources, SOP recorded higher nitrogen and phosphorus, potassium and zinc uptake (171.02 and 40.29, 197.73 kg ha-1 and 264.02 g ha-1 respectively) over MOP. The higher nitrogen, phosphorus, potassium and zinc uptake was recorded significantly in application of 50 per cent potassium at transplanting and 50 per cent at 30 DAT (168.52, 39.74, 195.69 kg ha-1 and 289.76 g ha-1 ) over 100 per cent potassium at transplanting (157.90, 37.03, 187.23 kg ha-1 and 277.73 g ha-1 ). Significantly higher nitrogen and phosphorus use efficiency was recorded in 200 per cent RDK (162.90 and 63.10%, respectively) over 100 per cent RDK. The higher potassium use efficiency on the contrary was recorded in 100 per cent RDK (121.18%) over 125, 150, 175 and 200 per cent RDK. Among the potassium sources, SOP recorded higher nitrogen, phosphorus and potassium use efficiency of onion plant (136.82, 53.72 and 107.89%, respectively) over MOP. The higher nitrogen, phosphorus and potassium use efficiency was recorded significantly in application of 50 per cent potassium at transplanting and 50 per cent at 30 DAT (134.82, 52.99 and 106.68%) over 100 per cent potassium at transplanting (126.31, 49.37 and 102.0%)

Effect of Potassium Levels, Sources and Time of Application on Storage Life of Onion (Allium cepa L.)

The present investigation on “Effect of potassium levels, sources and time of application on storage life of onion var. ArkaKalyan” was carried out at the College of Horticulture, Bagalkot, Karnataka during Kharif season crop of 2015 and 2016.The physiological loss in weight and rotting and sprouting of onion bulbs was minimum in 200 per cent RDK (19.25 and 13.91%, respectively) and maximum was recorded in 100 per cent RDK (23.40 and 18.13%, respectively). The marketable bulbs of onion was recorded highest in 200 per cent RDK (77.51%) and lowest marketable bulbs was recorded in 100 per cent RDK (71.96%) followed by 175 per cent RDK. The physiological loss in weight and rotting and sprouting of onion bulbs was minimum in potassium sources as SOP (21.12 and 15.09%) over MOP (22.60 and 16.50 per cent respectively). The marketable bulbs of onion was highest in potassium sources as SOP (76.01%, respectively) over MOP (74.43%). The increased marketable bulb yield and reduced the physiological loss in weight and rotting and sprouting onion bulb with the application 50 per cent potassium at transplanting and 50 per cent K at 30 DAT over 100 per cent potassium at transplanting

Response of Onion (Allium cepa L.) to Potassium Levels, Sources and Time of Application

The present investigation on “Effect of potassium levels, sources and time of application on growth and growth parameters of onion var. Arka Kalyan” was carried out at the College of Horticulture, Bagalkot, Karnataka during Kharif season of 2015 and 2016. Potassium significantly influenced the growth components like plant height, number of leaves per plant, leaf length, leaf breadth, neck thickness and biomass per plant with increasing levels of potassium at 30, 60 and 90 days after transplanting. Application of 200 per cent RDK recorded significantly higher plant height (37.08, 54.00 and 56.69 cm, respectively), number of leaves per plant (5.59, 8.66 and 9.88, respectively), leaf length (33.85, 47.50 and 48.02 cm, respectively), leaf breadth (5.19, 7.44 and 7.84 mm, respectively), leaf area per plant (154.52, 490.92 and 582.72 cm2, respectively), neck thickness (7.60, 13.90 and 15.08 mm, respectively) and biomass per plant (4.60, 8.22 and 14.70 g, respectively) and it proved significantly superior over 100 per cent RDK. Growth parameters like plant height, number of leaves per plant, leaf length, leaf breadth, leaf area per plant, neck thickness and biomass per plant varied significantly by potassium supplied as sulphate of potash over muriate of potash. The growth parameters was significantly influenced by the time of application of potassium. At 30 DAT, the application of 100 per cent potassium at transplanting was recorded significantly higher growth parameters compared to 50 per cent potassium at transplanting and 50 per cent K at 30 DAT was applied as basal. At 60 and 90 DAT, application of 50 per cent potassium at transplanting and 50 per cent K at 30 DAT was recorded superior growth over 100 per cent potassium at transplanting

Pengaruh Diversifikasi Pendapatan dan Bank Capital Buffer terhadap Kinerja dan Risiko Likuiditas pada Sektor Perbankan Syariah Indonesia

Penelitian ini bertujuan untuk mengetahui pengaruh diversifikasi pendapatan (DIVPEN) terhadap kinerja bank yang menggunakan proksi return on assets (ROA) dan return on equity (ROE) dan risiko likuiditas yang menggunakan proksi non performing financing (NPF) dengan mediasi bank capital buffer (BCB) pada sektor perbankan syariah Indonesia. Observasi dilakukan terhadap seluruh perbankan syariah yang terdaftar di Otoritas Jasa Keuangan salama periode 2009-2018. Metode analisisnya menggunakan analisis regresi linear berganda. Hasil penelitian menunjukkan bahwa DIVPEN memiliki pengaruh positif signifikan terhadap ROA dan ROE, namun berpengaruh negatif terhadap NPF. Sedangkan BCB memiliki pengaruh positif signifikan terhadap ROE dan pengaruh negatif signifikan terhadap NPF