THE RELATIONSHIP BETWEEN ATTACHMENT AND SERUM OXYTOCIN AND HEAT SHOCK PROTEIN-70 LEVELS IN ADOLESCENTS OF PARENTS WITH SCHIZOPHRENIA AND BIPOLAR DISORDER

Background: The aim of this study was to evaluate serum heat shock protein 70 (HSP70) and oxytocin levels, attachment and perceived social support levels in adolescents with parental bipolar disorder (BD) and Schizophrenia (SCZ). Subjects and Methods: This study included 9 adolescents with SCZ parents, 30 adolescents with BD parents and 31 healthy adolescents. Brief Symptom Inventory (BSI), Relationship Scale Questionnaire-Adolescent Form (RSQ-A) and Multidimensional Scale of Perceived Social Support (MSPSS) were administered to all participants. In addition, serum HSP-70 and oxytocin levels were evaluated. Results: There was no significant difference between the groups in terms of attachment style, psychiatric symptoms and perceived social support. Serum HSP-70 levels were found to be lower in adolescents whose parents had BD. Serum oxytocin levels of the SCZ group were significantly lower than those of the BD group. Conclusions: HSP-70 level was found to be lower in adolescents with BD parents. Oxytocin level was found to be lower in adolescents with SCZ parents. These findings suggest that HSP-70 and oxytocin may be a marker of early life stress in adolescents with parental psychopathology. However, studies are needed to evaluate the relationship between attachment, oxytocin and HSP-70 in adolescents exposed to parental psychopathology in early life

Antimicrobial, cytotoxic, antiviral wffects, and apectroscopic characterization of metabolites produced by fusarium oxysporum YP9B

The goal of the work is to determine the bioactive pharmaceutical metabolites produced by the Fusarium oxysporum YP9B isolate. Ten new natural compounds were isolated from the ethyl acetate extract of the F. oxysporum YP9B strain. Their structures were elucidated by spectroscopic methods using 1D and 2D NMR, UV, FT-IR, and mass spectra (LC-QTOF MS and GC-FID/MS). Identified compounds were named as; (1-benzyl-2-methoxy-2-oxoethyl)-2-hydroxy-3-methylbutanoate (1), 2-oxo-8-azatricyclo[9.3.1.1(3,7)]-hexadeca-1(15),3(16),4,6,11,13-hexaen-10-one (2), 2,3-dihydroxypropanoic, hexadecanoic anhydride (3a), 2,3-dihydroxypropanoic (9Z)-octadecenoic anhydride (3b), 2,3-dihydroxy-propanoic (9Z,12Z)-octadecadienoic anhydride (3c), 2,3-dihydroxypropanoic (11Z)-octadecenoic anhydride (4a), 2,3-dihydroxypropanoic, (9E,12E)-octadecadienoic anhydride (4b), 3-hydroxy-1,2,6,10-tetramethylundecyl hexzadecanoate (5a), 3-hydroxy-1,2,6,10-tetramethylundecyl (9E)-octadecaenoate (5b), and 3-hydroxy-1,2,6,10-tetramethylundecyl octadecanoate (5c). Antimicrobial activities of the isolates obtained from the YP9B strain were determined. Cytotoxic and antiviral activities were tested for the isolates against VERO, MCF-7, PC-3, and A549. Compounds 5a-c, 1, and 3a-c showed bacteriostatic activity at low concentrations, and 4a-b and 2 were found to be bactericides. MIC and MBC values against Mycobacterium smegmatis for the compounds 5a-c and 1 were determined to be <0.5 mu g/mL and 0.46 mu g/mL, respectively. The experimental result showed that compounds 2, 5a-c and 1 have strong cytotoxic (7.51 +/- 1.38 and 19.13 +/- 0.68 (mu M) IC50) activity. The antiviral activity against HSV type-1 was determined to be 1.25 mu M for compounds 4a-c and 0.312 mu M for compound 1

New Approach to Cancer: Anti-Angiogenic Treatment in Vitro Lung Cancer

WOS: 000464326000020Objective: Angiogenesis is physiological event which involve endothelial cells and in malignant conditions involve bone-marrow derived cells, stromal cells related to tumor microenvironment is a multifactorial event. The main factor in this action is vascular endothelial growth factor (VEGFA). In recent years, bevacizumab (anti-VEGF) agent has been used against VEGFA receptor to block potential vascular signal in tumor cells and different activities were determined by in vitro experiments in lung cancer (NSCLC), colorectal cancer, breast cancer and renal cell carcinoma. The main objective of this study was to determine the vascularity and mechanism of vascular cancer in Bevacizumab-treated lung cancer cells compared to normal cells. Methods: Study of cell functions with main stimulant VEGF and Bevacizumab applications which provide angiogenesis blockade. Normal epithelial HUVEC line and lung cancer A549 cell line were used and cultured according to ATCC protocol. After application of different doses of agents, cell viability, Sytox proliferation index and Notch pathway activity responsible for angiogenesis were investigated. Results: Cells treated with VEGF showed a significant increase in cell viability with incubation for 24 hours. VEGF effect was significantly different in two cell lines compared to viability. In the application of Bevacizumab, the proliferation index of A549 cells decreased significantly and the ADAM10 protein level associated with the Notch mechanism was found to increase significantly compared to HUVEC cell. Conclusion: According to the findings, it is seen that it needs more detailed studies to explain the molecular mechanism of angiogenesis. In addition to conventional cancer treatment methods, enhancing combined therapies with known antiangiogenic agents will increase the chances of success

The Systemic Cell Apoptotic-Based Neutrophil– Lymphocyte Ratio: Experience in Children Diagnosed with ADHD and Autism Spectrum Disorder

Objective: In recent years, the rate of Neutrophil/Lymphocyte ratio (NLR) has been shown to be a marker of systemic inflammation that associated with prognosis in many diseases like malignancies and chronic inflammatory diseases. Based on previous studies, there is not any finding about NLR and cellular morphological mechanism studied together in inflamation-related diseases; attention deficit hyperactivity disorder(ADHD) and autism spectrum disorders(ASD). We assessed the effect and association of these parameters on ethiopathogenesis of neurodevelopmental disorders.Methods: 30 healthy and 30 each patients who were diagnosed with ADHD and ASD were evaluated at psychiatry department in tertiary hospital. The hemogram profile were analyzed and NLR parameter was statistically evaluated among groups. However, apoptotic stage of cells were staining with 2 different methods. Apoptotic mechanism of ADHD, ASD and control group were comparably displayed.Results: NLR values in patients diagnosed with ADHD and ASD were significantly higher compare to control; lymphocyte count was found significantly lower level in patient groups. Apoptotic morphology becomes evident as degree of disease increment.Conclusions: This parameter can be used as an easily applicable method is estimated to be risk for psychiatric diseases. The positive association of NLR with apoptotic imaging indicates a marker of cellular degradation with neurodegenerative disorders.Amaç: Son yıllarda, Nötrofil / Lenfosit oranının (NLR) sistemik inflamasyonun bir belirteci olduğu gösterilmiştir ve maligniteler ve kronik inflamatuar hastalıklar gibi birçok hastalıkta prognoz ile ilişkilidir. Önceki çalışmalara dayanarak, NLR ve hücresel morfolojik mekanizmanın inflamasyonla ilişkili hastalıklarda dikkat eksikliği hiperaktivite bozukluğu (DHB) ve otizm spektrum bozuklukları (ASD)’nın birlikte çalışıldığı bir bulgu yoktur. Araştırmamızda bu parametrelerin nörogelişimsel bozukluklar etiyopatogenezine etkisini ve ilişkisini değerlendirdik. Gereç ve Yöntem: DHB ve ASD tanısı alan 30 sağlıklı ve 30 hasta, üçüncü basamak bir hastanede psikiyatri kliniğinde değerlendirildi. Tüm gruplarda hemogram profiline bakılarak NLR sayısı istatistiksel olarak karşılaştırıldı. Ayrıca hücrelerin apoptotik evresini belirlemek üzere 2 farklı yöntemle boyandı. DHB, ASD ve kontrol grubunun apoptotik mekanizması karşılaştırılabilir şekilde görüntülendi. Bulgular: DHB ve ASD tanısı alan hastalarda NLR değerleri kontrole kıyasla anlamlı derecede yüksekti; lenfosit sayısı hasta gruplarında anlamlı olarak düşük bulundu. Apoptotik morfoloji, hastalık artışı derecesi ile korele olarak farklılık gösterdi. Sonuç: Bu parametre, psikiyatrik hastalıklara yakalanma riski olan durumlarda, erken tanıda kolay uygulanabilir bir yöntem olarak kullanılabilir. Nötrofil/Lenfosit oranının apoptotik görüntüleme ile pozitif ilişkisi, nörodejeneratif patolojilerde hücresel bozulmanın bir belirtecini gösterir.WOS:00062876870001

ANTIOXIDANT, ANTIMICROBIAL AND ANTITUMORAL EFFECTS OF STACHYS ANNUA (L.) L. SUBSP. ANNUA VAR. ANNUA IN COMPARATIVE CANCER PROFILES

The genus Stachys (Lamiaceae) have been used for the treatment of folk medicine. This study was carried out with an objective to investigate the in vitro antimicrobial, antioxidant and antitumor effects of leaves of Stachys annua subsp. annua var. annua. The antimicrobial activity of ethanol extract (75µL) of leaves of S. annua subsp. annua var. annua was evaluated for potential antimicrobial activity against some bacterial and fungal isolates obtained from hospital. Antimicrobial susceptibility testing was determined with using the disc diffusion method according to the protocol applied by CLSI. The results showed that the ethanol extract revealed similarly effect against bacteria as compared to standart antibiotics. But, the extract showed slightly effect against Candida species. Also, we used HeLa and PC3 cells to test anticancerogen effect on urinary system. The cells were cultured for ATCC protocol. After application of extract dilution, we analyzed MTT assay for viability detection at 570 nm. Based on cytotoxicity value, optimal concentration were evaluated at 50uM. The potency of cell growth inhibition for each extract was expressed as IC50 value. According to datas, we showed that antioxidant activity of this plant have significantly increased. Stachys annua (L.) L. subsp. annua var. annua may evaluated in advanced pharmacological studies for distinct feature

Effective copper(II) and nickel(II) complexes with N3O and ON3 thiosemicarbazidato ligands. Synthesis, structural analysis and in vitro cytotoxity on melanoma B16F10 cells

Novel copper(II) and nickel(II) complexes were newly synthesized by template condensation using 2-amino-3,5-dibromo- or 2-hydroxy-5-bromo-benzaldehyde S-propyl thiosemicarbazones as starting materials. The complexes with asymmetric donor atom set were identified through use of analytical and spectroscopic methods (IR, H NMR, ESI-MS and X-ray crystallography). Cytotoxicity potents of the N3O and ON3-type complexes were determined on melanoma B16F10 and fibroblast BJ ATCC CRL-2522 cell lines. The cell lines were exposed to the complexes in the concentration range of 6-500 mu M for 24 h. The results indicated selective effect of the nickel and copper complexes in favor of healthy cells. Namely, relative low concentrations of the test molecules resulted in cytotoxic effects on malignant cells while higher concentrations were required for normal cell death. The nickel complexes were more effective than the copper complexes and a nickel complex (1a) showed selectively cytotoxcity by IC50 value of 1978 mu g/ml on B16F10 cells compared to the 5464 mu g/ml value of CRL-2522 cells

The investigation antiproliferative and apoptotic effects of capsaicin and alpha lipoic acid on colon adenocarcinoma cells

WOS: 000431624200026

A NEW MARKER PERILIPINE COUNTERWORK WITH VITAMIN D THROUGH REGULATION LIPID METABOLISM IN OBESES

Introduction: Vitamin D deficiency and obesity are common controversial health problems. Vit D supports lipolysiv from the outside, stimulates lipogenesis. Perilipine protein, which allows the use of lipids in the mitochondria in cell. Our main aim of this research was to control obesity prognosis monitoring Perilipine levels related by WO treatments. Methods: During winter months, patients that have admitted the hospital whose ages ranged from 18 to 65 years at 80 obese and normal BMI individuals were included the study. Blood samples from patients were taken and measured for VitD level. Experimental groups arranged again according to BMI and VitD results. Results: The mean age of the patients was +/- 34(18-65). After organized samples, vitamin D deficiency group which 25-OH D levels were < 20 nglml, were 37.75 +/- 4.79 higher Perilipine levels than normal VItD individuals. However, Perilipine levels were increased in obese patients display positive correlation with BMI. Discussion: These results were indicated that high frequency of vitamin D deficiency drives obesity relatively especially in winter months. The research findings suggest that Perilipine expression has direct correlation by vitamin D alteration. For this purpose, Perilipine can be a novel marker to prevent obesity and should he controlled previously identified obesity groups treated new methods of VitD uptake.WOS:0005261216000152-s2.0-8508339293