The genus Stachys (Lamiaceae) have been used for the treatment of folk medicine. This study was carried out with an objective to investigate the in vitro antimicrobial, antioxidant and antitumor effects of leaves of Stachys annua subsp. annua var. annua. The antimicrobial activity of ethanol extract (75µL) of leaves of S. annua subsp. annua var. annua was evaluated for potential antimicrobial activity against some bacterial and fungal isolates obtained from hospital. Antimicrobial susceptibility testing was determined with using the disc diffusion method according to the protocol applied by CLSI. The results showed that the ethanol extract revealed similarly effect against bacteria as compared to standart antibiotics. But, the extract showed slightly effect against Candida species. Also, we used HeLa and PC3 cells to test anticancerogen effect on urinary system. The cells were cultured for ATCC protocol. After application of extract dilution, we analyzed MTT assay for viability detection at 570 nm. Based on cytotoxicity value, optimal concentration were evaluated at 50uM. The potency of cell growth inhibition for each extract was expressed as IC50 value. According to datas, we showed that antioxidant activity of this plant have significantly increased. Stachys annua (L.) L. subsp. annua var. annua may evaluated in advanced pharmacological studies for distinct feature
