Common Mitochondrial DNA Mutations Generated through DNA-Mediated Charge Transport

Mutation sites that arise in human mitochondrial DNA as a result of oxidation by a rhodium photooxidant have been identified. HeLa cells were incubated with [Rh(phi)2bpy]Cl3 (phi is 9,10-phenanthrenequinone diimine), an intercalating photooxidant, to allow the complex to enter the cell and bind mitochondrial DNA. Photoexcitation of DNA-bound [Rh(phi)2bpy]3+ can promote the oxidation of guanine from a distance through DNA-mediated charge transport. After two rounds of photolysis and growth of cells incubated with the rhodium complex, DNA mutations in a portion of the mitochondrial genome were assessed via manual sequencing. The mutational pattern is consistent with dG to dT transversions in the repetitive guanine tracts. Significantly, the mutational pattern found overlaps oxidative damage hot spots seen previously. These mutations are found within conserved sequence block II, a critical regulatory element involved in DNA replication, and these have been identified as sites of low oxidation potential to which oxidative damage is funneled. On the basis of this mutational analysis and its correspondence to sites of long-range oxidative damage, we infer a critical role for DNA charge transport in generating these mutations and, thus, in regulating mitochondrial DNA replication under oxidative stress

Effects of forage supplements on milk production and chemical properties, in vivo digestibility, rumen fermentation and N excretion in dairy cows offered red clover silage and corn silage or dry ground corn

peer-reviewedThis study concerned the effects of partial substitution of clover silage with high starch forages on milk production and chemical composition, in vivo digestibility, rumen fermentation pattern and nitrogen excretion of dairy cows. Sixteen dairy cows were separated into two groups and were assigned to treatments in a two-period crossover design. Two forage supplements were used: corn silage (CS) and dry ground corn (DG). All animals received 4.5 kg of concentrate dry matter per day. Results showed no significant difference between the forage supplements for milk production, while significant differences (P<0.01) were observed for milk fat, milk protein and nitrogen utilisation efficiency (42 v. 4.0 g/kg, 3.5 v. 3.3 g/kg and 222 v. 188 g/kg, respectively, for DG and CS). Faecal N excretion did not differ between forage supplements, but urinary N excretion was higher for CS (P<0.05). No significant differences were observed between treatments for rumen fluid pH or for rumen fluid concentrations of ammonium nitrogen or of acetic, propionic or butyric acids. Dry matter intake and the in vivo digestibility of dry matter, organic matter, acid detergent fibre and neutral detergent fibre were all higher for CS compared with DG.The authors are grateful to the Environmental Office of the Cantabrian Government for funding the project 05-640.02-2174

Effects of anisotropy in spin molecular-orbital coupling on effective spin models of trinuclear organometallic complexes

We consider layered decorated honeycomb lattices at two-thirds filling, as realized in some trinuclear organometallic complexes. Localized $S=1$ moments with a single-spin anisotropy emerge from the interplay of Coulomb repulsion and spin molecular-orbit coupling (SMOC). Magnetic anisotropies with bond dependent exchange couplings occur in the honeycomb layers when the direct intracluster exchange and the spin molecular-orbital coupling are both present. We find that the effective spin exchange model within the layers is an XXZ + 120$^\circ$ honeycomb quantum compass model. The intrinsic non-spherical symmetry of the multinuclear complexes leads to very different transverse and longitudinal spin molecular-orbital couplings, which greatly enhances the single-spin and exchange coupling anisotropies. The interlayer coupling is described by a XXZ model with anisotropic biquadratic terms. As the correlation strength increases the systems becomes increasingly one-dimensional. Thus, if the ratio of SMOC to the interlayer hopping is small this stabilizes the Haldane phase. However, as the ratio increases there is a quantum phase transition to the topologically trivial `$D$-phase'. We also predict a quantum phase transition from a Haldane phase to a magnetically ordered phase at sufficiently strong external magnetic fields.Comment: 22 pages, 11 figures. Final version of paper to be published in PRB. Important corrections to appendix

Low-thrust chemical propulsion system propellant expulsion and thermal conditioning study. Executive summary

Preferred techniques for providing abort pressurization and engine feed system net positive suction pressure (NPSP) for low thrust chemical propulsion systems (LTPS) were determined. A representative LTPS vehicle configuration is presented. Analysis tasks include: propellant heating analysis; pressurant requirements for abort propellant dump; and comparative analysis of pressurization techniques and thermal subcoolers

Late Pleistocene and Holocene palaeoenvironments in and around the middle Caspian basin as reconstructed from a deep-sea core

This article has been made available through Open Access by the Brunel Open Access Publishing Fund.Late Pleistocene and/or Holocene high-resolution palynological studies are available for the south basin of the Caspian Sea (CS), the world's largest lake. However, the north and middle basins have not been the object of high-resolution palynological reconstructions. This new study presents the pollen, spores and dinoflagellate cysts records obtained from a 10m-long sediment core recovered in the middle basin, which currently has brackish waters and is surrounded by arid and semi-arid vegetation.An age-depth model built based on six radiocarbon dates on ostracod shells indicates that the sequence spans the period from 14.47 to 2.43cal.kaBP. The present palaeoenvironmental study focuses on the top 666cm, or from 12.44 to 2.43cal.kaBP.At the vegetation level, the Younger Dryas is characterised by an open landscape dominated by desert vegetation composed by Amaranthaceae with shrubs and salt-tolerant plants. However, although the Early Holocene is also characterised by desert vegetation, it is enriched in various shrubs such as Ephedra and Calligonum, but tree expansion is not important at the Holocene onset. After a major shift at 8.19cal.kaBP, the Middle Holocene displays now both the character of desert and of steppe, although some trees such as Quercus and Corylus slightly spread. The Late Holocene records steppe vegetation as dominant, with more tree diversity.Regarding the lacustrine signal, the dinocyst assemblage record fluctuates between slightly brackish conditions highlighted by Pyxidinopsis psilata and Spiniferites cruciformis, and more brackish ones - similar to the present day - with the dominance of Impagidinium caspienense. The Late Pleistocene is characterised by low salinities, related to the Khvalynian highstand. From 11.56cal.kaBP, slightly more saline waters are reconstructed with an increase of I.caspienense for a period of 1000 years, which could be attributed to the Mangyshlak lowstand. From 10.55cal.kaBP, low salinity conditions return with remains such as Anabaena and Botryococcus abundant until 8.83cal.kaBP, followed by a slow, progressive decrease of P. psilata and S. cruciformis until 4.11cal.kaBP, which is the main assemblage change at lacustrine scale. Since then, higher salinities, similar to the present one, are reconstructed. Finally, Lingulodinium machaerophorum starts its development only at 2.75cal.kaBP, in the Late Holocene.The present research revealed fundamental differences from previously published sea-level curves, in that a 6000yr-long highstand suggested by low salinities is shown between 10.55 and 4.11cal.kaBP. Amongst other arguments, using a comparison to a similar palynological regard but in the south basin, a N-S salinity gradient that is the reverse of the present one across the CS, suggests that the Amu Darya was flowing in the CS. Hence the CS levels during the Late Pleistocene and Holocene were influenced by a combination of precipitation over the high European latitudes and the indirect influence of the Indian summer monsoon over the Pamirs. © 2014.This study has been conducted within the European Contract INCO-Copernicus “Understanding the Caspian Sea erratic fluctuations” n IC15-CT96-0112. This was funded by the Centre National de la Recherche Scientifique within the framework of the INSUDYTEC (DYnamique de la Terre et du Climat) Program (France)