Gathering and Analyzing Surface Parameters for Diet Identification Purposes

International audienceModern surface acquisition devices, such as interferometers and confocal microscopes, make it possible to have accurate three-dimensional (3D) numerical representations of real surfaces. The numerical dental surfaces hold details that are related to the microwear that is caused by food processing. As there are numerous surface parameters that describe surface properties and knowing that a lot more can be built, is it possible to identify the ones that can separate taxa based on their diets? Until now, the candidates were chosen from among those provided by metrology software, which often implements International Organization for Standardization (ISO) parameters. Moreover, the way that a parameter is declared as diet-discriminative differs from one researcher to another. The aim of the present work is to propose a framework to broaden the investigation of relevant parameters and subsequently a procedure that is based on statistical tests to highlight the best of them. Many parameters were tested in a previous study. Here, some were dropped and others added to the classical ones. The resulting set is doubled while considering two derived surfaces: the initial one minus a second order and an eighth order polynomial. The resulting surfaces are then sampled—256 samples per surface—making it possible to build new derived parameters that are based on statistics. The studied dental surfaces belong to seven sets of three or more groups with known differences in diet. In almost all cases, the statistical procedure succeeds in identifying the most relevant parameters to reflect the group differences. Surprisingly, the widely used Area-scale fractal complexity (Asfc) parameter—despite some improvements—cannot differentiate the groups as accurately. The present work can be used as a standalone procedure, but it can also be seen as a first step towards machine learning where a lot of training data is necessary, thus making the human intervention prohibitive

L'écologie alimentaire d'Eucladoceros ctenoides : un outil pour suivre les variations environnementales régionales en Europe au Pléistocène inférieur

The early Pleistocene is represented by a succession of glacial-interglacial cycles character-ized by a general tendency towards global cooling, with increasing aridity and seasonality.The large deer Eucladoceros is found in abundance in Europe during this period of faunaldispersions. The dietary plasticity of Eucladoceros and how it can mirror early Pleistoceneclimatic variations will be explored here using Dental Microwear Texture Analysis. Thewide range of dental microwear textures for Eucladoceros reflects a low selectivity and highplasticity in its diet. It is an appropriate proxy to track vegetal resource availability. Oscil-lations were identified between a browsing and a grazing signal. This study proposes thata browsing signal is associated with a fossil assemblage deposited during an interglacialevent characterized by warmer temperatures and deciduous vegetation. A grazing signalmore likely indicates a glacial event with cooler temperatures and a developed herbaceous,bushy layer.Le Pléistocène inférieur correspond à une succession de cycles glaciaires-interglaciairescaractérisée par une tendance générale au refroidissement global, avec une aridité et unesaisonnalité croissantes. Le cervidé de grande taille Eucladoceros est abondant en Europedurant cette période de dispersions fauniques. Grâce à l'analyse de texture de la micro-usuredentaire, nous explorons la plasticité alimentaire d'Eucladoceros et la manière dont celle-ci reflète les variations climatiques du Pléistocène inférieur. La large gamme de texturesde micro-usure dentaire d'Eucladoceros reflète sa faible sélectivité et sa forte plasticité ali-mentaire. De fait, il constitue un outil approprié pour aborder la disponibilité en ressources végétales dans le milieu. Nous identifions des oscillations entre un signal brouteur et unsignal paisseur. Nous proposons qu'un signal brouteur soit associé à un assemblage fossiledéposé lors d'un évènement interglaciaire caractérisé par des températures plus chaudeset une végétation décidue, tandis qu'un signal paisseur correspondrait plutôt à un évène-ment glaciaire, avec des températures plus froides et la présence d?une strate herbacée etarbustive développée.This project was supported by the “Agence nationale dela recherche” (ANR TRIDENT: grant number: ANR-13-JSV7-0008-01; PI.: Gildas Merceron)

The Primate Community of Cachoeira (Brazilian Amazonia): A Model to Decipher Ecological Partitioning among Extinct Species

International audienceDental microwear analysis is conducted on a community of platyrrhine primates from South America. This analysis focuses on the primate community of Cachoeira Porteira (Para, Brazil), in which seven sympatric species occur: Alouatta seniculus, Ateles paniscus, Cebus apella, Chiropotes satanas, Pithecia Pithecia, Saguinus midas, and Saimiri sciureus. Shearing quotients are also calculated for each taxon of this primate community. Dental microwear results indicate significant differences between taxa, but are somewhat insufficient when it comes to discriminating between ecologically similar taxa. The primates of Cachoeira Porteira all incorporate a certain amount of fruit in their diet, entailing a definite amount of interspecific competition as they must share food resources. Alouatta is the most folivorous taxon of this community, which is corroborated by dental microwear analysis. Ateles, although of a similar size to Alouatta, limits inter-specific competition by incorporating more fruit in its diet. Cebus has a very diverse omnivorous diet, which is highlighted in this study, as it compares to both fruit and leaf eating taxa. In some cases, microwear results need to be supplemented by other methods. For example, dental microwear seems insufficient to distinguish between Pithecia and Chiropotes, which eat foods with similar physical properties. However, other methods (i.e. shearing quotients and body mass) provide enough complimentary information to be able to highlight differences between the two taxa. On the other hand, dental microwear can highlight differences between primates which have similar diets, such as Saimiri and Saguinus. In this case, differences could be due to other exogenous factors

Can Dental Microwear Textures Record Inter-Individual Dietary Variations?

International audienceBackground: Dental microwear analyses are commonly used to deduce the diet of extinct mammals. Conventional methods rely on the user identifying features within a 2D image. However, recent interdisciplinary research has lead to the development of an advanced methodology that is free of observer error, based on the automated quantification of 3D surfaces by combining confocal microscopy with scale-sensitive fractal analysis. This method has already proved to be very efficient in detecting dietary differences between species. Focusing on a finer, intra-specific scale of analysis, the aim of this study is to test this method's ability to track such differences between individuals from a single population. Methodology/Principal Findings: For the purposes of this study, the 3D molar microwear of 78 individuals from a well-known population of extant roe deer (Capreolus caprelous) is quantified. Multivariate statistical analyses indicate significant seasonal and sexual differences in individual dental microwear design. These are probably the consequence of seasonal variations in fruit, seed and leaf availability, as well as differences in feeding preference between males and females due to distinct energy requirements during periods of rutting, gestation or giving birth. Nevertheless, further investigations using two-block Partial Least-Squares analysis show no strong relationship between individual stomach contents and microwear texture. This is an expected result, assuming that stomach contents are composed of food items ingested during the last few hours whereas dental microwear texture records the physical properties of items eaten over periods of days or weeks. Conclusions/Significance: Microwear 3D scale-sensitive fractal analysis does detect differences in diet ranging from the inter-feeding styles scale to the intra-population between-season and between-sex scales. It is therefore a possible tool, to be used with caution, in the further exploration of the feeding biology and ecology of extinct mammals

Spatio-temporal diversity of dietary preferences and stress sensibilities of early and middle Miocene Rhinocerotidae from Eurasia: impact of climate changes

Major climatic and ecological changes are documented in terrestrial ecosystems during the Miocene epoch. The Rhinocerotidae are a very interesting clade to investigate the impact of these changes on ecology, as they are abundant and diverse in the fossil record throughout the Miocene. Here, we explored the spatio-temporal evolution of rhinocerotids’ paleoecology during the early and middle Miocene of Europe and Pakistan. We studied the dental texture microwear (proxy for diet) and enamel hypoplasia (stress indicator) of 19 species belonging to four sub-tribes and an unnamed clade of Rhinocerotidae, and coming from nine Eurasian localities ranging from Mammal Neogene zone (MN) 2 to MN7/8. Our results suggest clear differences in the feeding ecology and thus niche partitioning at Kumbi 4 (MN2, Pakistan), Sansan (MN6, France), and Villefranche d’Astarac (MN7/8, France), while overlap of the interpreted diets and subtle variations are discussed for Béon 1 (MN4, France) and Gračanica (MN5/6, Bosnia-Herzegovina). All rhinocerotids studied were interpreted as browsers or mixed-feeders, and none had a grazer nor frugivore diet. The prevalence of hypoplasia was moderate (∼10%) to high (> 20%) at all localities but Kumbi 4 (∼6%), and documented quite well the local conditions. For instance, the high prevalence at the close to Miocene Climatic Optimum locality of Béon 1 (∼26%) has been correlated with periodical droughts, while the moderate ones (∼10%) at Sansan and Devínska Nová Ves Spalte (Slovakia) both dated from the MN6 (i.e., by the middle Miocene Climatic Transition, ca. 13.9 Mya) were linked to the persistence of sub-tropical local conditions. Besides locality, species and tooth locus were also important factors of variation for the prevalence of hypoplasia. The very large hippo-like Brachypotherium brachypus was one of the most affected species at all concerned localities (but Sansan), whereas early-diverging elasmotheriines were very little affected, suggesting an influence of phylogeny and/or diet in stress susceptibility

Deer in an arid habitat: dental microwear textures track feeding adaptability

Teeth constitute a bridge between an organism and its environment. Dental wear is a good proxy for (paleo) ecologists to better comprehend the ecology and habitat of modern and extinct species. In this study, we showed Dental Microwear Texture Analysis to be a useful tool, integrating not only specific plant selection but also dietary quality and digestibility in order to understand resource use. Resource-partitioning, seasonal and sexual variations in the diet of two deer species on a Spanish game estate are explored here through Dental Microwear Texture Analysis. This Mediterranean área is on the fringes of the average European environments in terms of constraints and diet for extant red and fallow deer, resulting in an opportunity to understand their ability to live in harsh conditions and the feeding strategies they developed. These two taxa already experienced harsh living conditions during the Pleistocene. Dental microwear texture shows both deer feeding differently on the herbaceous layer in a context where it is the main resource consumed annually. These differences are linked to body mass. With its smaller incisor arcade, Dama dama is able to be more selective, hence focusing on less fibrous parts of forages. Cervus elaphus is more plastic, with dietary variations corresponding to seasonal plant availability and the physiological requirements of stags and does. In general, C. elaphus consumes a more fibrous and less digestible vegetable material tan D. dama. This study brings light on the feeding behavior of the two game species under constraint conditions. The results of this study are discussed in terms of realized vs potential ecological niches.This work was supported by the Agence Nationale de la Recherche (ANR TRIDENT: grant number: ANR-13-JSV7-0008-01; PI: Gildas MERCERON) and the project P07-RNM-03087 from the Consejería de Innovacion Ciencia y Empresa, Junta de Andalucía, by the Organismo Autonomo de Parques Nacionales, Ministerio de Medio Ambiente, Medio Rural y Marino, Spain, and by the European Fund for Regional Development (FEDER)

Surface texture analysis in Toothfrax and MountainsMap® SSFA module: Different software packages, different results?

Pre-print: Calandra_etal_SSFA_PCIarchaeo_revised.pdf (figures incorporated, but additionally available as separate PDF files) Supplementary Material: Bayesian-models_PCIarchaeo_revised.pdf and Comparison-analyses_PCIarchaeo_revised.pdf See also related identifiers for the other supplementary materials. Revised version submitted to PCI Archaeology

Palaeogenomics of Upper Palaeolithic to Neolithic European hunter-gatherers

Modern humans have populated Europe for more than 45,000 years1,2. Our knowledge of the genetic relatedness and structure of ancient hunter-gatherers is however limited, owing to the scarceness and poor molecular preservation of human remains from that period3. Here we analyse 356 ancient hunter-gatherer genomes, including new genomic data for 116 individuals from 14 countries in western and central Eurasia, spanning between 35,000 and 5,000 years ago. We identify a genetic ancestry profile in individuals associated with Upper Palaeolithic Gravettian assemblages from western Europe that is distinct from contemporaneous groups related to this archaeological culture in central and southern Europe4, but resembles that of preceding individuals associated with the Aurignacian culture. This ancestry profile survived during the Last Glacial Maximum (25,000 to 19,000 years ago) in human populations from southwestern Europe associated with the Solutrean culture, and with the following Magdalenian culture that re-expanded northeastward after the Last Glacial Maximum. Conversely, we reveal a genetic turnover in southern Europe suggesting a local replacement of human groups around the time of the Last Glacial Maximum, accompanied by a north-to-south dispersal of populations associated with the Epigravettian culture. From at least 14,000 years ago, an ancestry related to this culture spread from the south across the rest of Europe, largely replacing the Magdalenian-associated gene pool. After a period of limited admixture that spanned the beginning of the Mesolithic, we find genetic interactions between western and eastern European hunter-gatherers, who were also characterized by marked differences in phenotypically relevant variants