Infrared Imaging of Meibomian Glands and Evaluation of the Lipid Layer in Sjogren's Syndrome Patients and Nondry Eye Controls

Menzies, K. L., Srinivasan, S., Prokopich, C. L., & Jones, L. (2015). Infrared Imaging of Meibomian Glands and Evaluation of the Lipid Layer in Sjogren’s Syndrome Patients and Nondry Eye Controls. Investigative Ophthalmology & Visual Science, 56(2), 836–841. https://doi.org/10.1167/iovs.14-13864Purpose.: The purpose of this study was to evaluate meibomian gland dropout and lipid layer thickness (LLT) in patients with and without Sjögren's syndrome dry eye (SS). Methods.: We recruited 11 participants with SS (males/females [M/F], 1:10; mean age = 56.0 ± 9.1 years) and 10 control subjects without dry eye (M/F, 3:7; mean age = 58.5 ± 4.7 years). All participants completed the Ocular Surface Disease Index (OSDI) questionnaire. The LLT was assessed using the Tearscope Plus based on the appearance of the lipid layer. Noninvasive tear break-up time (NITBUT) also was measured. The lower and upper lids were everted, and the meibomian glands were imaged using the infrared camera of the Keratograph 4. A meibomian gland dropout score due to gland loss was obtained. Statistical analysis was conducted using the Mann-Whitney U test and correlations were determined using Spearman rank correlations. Results.: Of the SS participants, 100% reported ocular and oral dryness symptoms in the AECC questionnaire. The SS group recorded a higher OSDI score (median = 48.00, interquartile range [IQR] 23.0–56.2 vs. 2.1, IQR 0.0–2.6; P < 0.001), reduced LLT (median [IQR] = 15.0 [15.0–15.0] vs. 60.0 [45.0–100.0] nm; P = 0.001), and lower NITBUT (median [IQR] = 3.7 [2.5–4.2] vs. 9.5 [6.4–17.6] sec; P < 0.001) compared to the controls. Digital meibomian gland dropout score (% dropout) was significantly higher for the SS group (16.0% [IQR 12.1–40.0%] vs. 6.7% [IQR 1.5–12.7%]; P = 0.01). Subjective meibomian gland dropout score (0–6 score) was significantly higher for the SS group (median [IQR] = 1.5 [1.0–4.0] vs. 1.0 [0.0–1.25]; P = 0.03). Conclusions.: Patients with SS showed higher meibomian gland dropout scores and reduced LLT and NITBUT, which likely contribute to the severe dry eye symptoms reported by SS subjects

Design and Synthesis of Broad Spectrum Trypanosomatid Selective Inhibitors

Neglected tropical diseases caused by parasitic infections are an ongoing and increasing concern that have a devastating effect on the developing world due to their burden on human and animal health. In this work, we detail the preparation of a focused library of substituted-tetrahydropyran derivatives and their evaluation as selective chemical tools for trypanosomatid inhibition and the follow-on development of photoaffinity probes capable of labeling target protein(s) <i>in vitro</i>. Several of these functionalized compounds maintain low micromolar activity against <i>Trypanosoma brucei</i>, <i>Trypanosoma cruzi</i>, <i>Leishmania major</i>, and <i>Leishmania donovani</i>. In addition, we demonstrate the utility of the photoaffinity probes for target identification through preliminary cellular localization studies

The historical vanishing of the Blazhko effect of RR Lyr from GEOS and Kepler surveys

RR Lyr is one of the most studied variable stars. Its light curve has been regularly monitored since the discovery of the periodic variability in 1899. Analysis of all observed maxima allows us to identify two primary pulsation states defined as pulsation over a long (P0 longer than 0.56684 d) and a short (P0 shorter than 0.56682 d) primary pulsation period. These states alternate with intervals of 13-16 yr, and are well defined after 1943. The 40.8 d periodical modulations of the amplitude and the period (i.e. Blazhko effect) were noticed in 1916. We provide homogeneous determinations of the Blazhko period in the different primary pulsation states. The Blazhko period does not follow the variations of P0 and suddenly diminished from 40.8 d to around 39.0 d in 1975. The monitoring of these periodicities deserved and deserves a continuous and intensive observational effort. For this purpose we have built dedicated, transportable and autonomous small instruments, Very Tiny Telescopes (VTTs), to observe the times of maximum brightness of RR Lyr. As immediate results the VTTs recorded the last change of P0 state in mid-2009 and extended the time coverage of the Kepler observations, thus recording a maximum O-C amplitude of the Blazhko effect at the end of 2008, followed by the historically smallest O-C amplitude in late 2013. This decrease is still ongoing and VTT instruments are ready to monitor the expected increase in the next few years.Comment: 10 pages, 6 figures. Accepted for publication in MNRAS. Contents of appendix B may be requested to first autho

Meeting the Cool Neighbours, II: Photometry of southern NLTT stars

We present BVRI photometry of 180 bright, southern nearby-star candidates. The stars were selected from the New Luyten Two-Tenths proper motion catalogue based on optical/infrared colours, constructed by combining Luytens's m(r) estimates with near-infrared photometry from the 2-Micron All Sky Survey. Photometric parallaxes derived from (V-K), (V-I) and (I-J) colours, combined with the limited available astrometry, show that as many as 108 stars may lie within 20 parsecs of the Sun. Of these, 53 are new to nearby star catalogues, including three within 10 parsecs of the Sun.Comment: 24 pages, 6 figures, to be published in The Astronomical Journal. More information can be found at http://www.stsci.edu/~inr/nstars.htm

UBVI Surface Photometry of the Spiral Galaxy NGC 300 in the Sculptor Group

We present UBVI surface photometry for 20.'5 X 20.'5 area of a late-type spiral galaxy NGC 300. In order to understand the morphological properties and luminosity distribution characteristics of NGC 300, we have derived isophotal maps, surface brightness profiles, ellipticity profiles, position angle profiles, and color profiles. By merging the I-band data of our surface brightness measurements with those of Boeker et al. (2002) based on Hubble Space Telescope observations, we have made combined I-band surface brightness profiles for the region of 0."02 < r < 500" and decomposed the profiles into three components: a nucleus, a bulge, and an exponential disk.Comment: 16 pages(cjaa209.sty), Accepted by the Chinese J. Astron. Astrophys., Fig 2 and 8 are degraded to reduce spac

Limits on Stellar and Planetary Companions in Microlensing Event OGLE-1998-BUL-14

We present the PLANET photometric data set for \ob14, a high magnification ($A_{\rm max}\sim 16$) event alerted by the OGLE collaboration toward the Galactic bulge in 1998. The PLANET data set consists a total of 461 I-band and 139 $V-$band points, the majority of which was taken over a three month period. The median sampling interval during this period is about 1 hour, and the $1\sigma$ scatter over the peak of the event is 1.5%. The excellent data quality and high maximum magnification of this event make it a prime candidate to search for the short duration, low amplitude perturbations that are signatures of a planetary companion orbiting the primary lens. The observed light curve for \ob14 is consistent with a single lens (no companion) within photometric uncertainties. We calculate the detection efficiency of the light curve to lensing companions as a function of the mass ratio and angular separation of the two components. We find that companions of mass ratio $\ge 0.01$ are ruled out at the 95% confidence level for projected separations between 0.4-2.4 \re, where \re is the Einstein ring radius of the primary lens. Assuming that the primary is a G-dwarf with \re\sim3 {\rm AU} our detection efficiency for this event is $\sim 60$ for a companion with the mass and separation of Jupiter and $\sim5$ for a companion with the mass and separation of Saturn. Our efficiencies for planets like those around Upsilon And and 14 Her are > 75%.Comment: Data available at http://www.astro.rug.nl/~planet/planetpapers.html 20 pages, 10 figures. Minor changes. ApJ, accepte