The effect of flowering stage in wheat on the infection efficiency of Ustilago tritici

Dans l'ouest du Canada, la détermination du niveau de résistance de lignées de blé (Triticum aestivum) à l’ Ustiiago tritici est habituellement faite par inoculation lorsque les plantes atteignent le stade mi-anthèse du développement de l'épi. Par contre, il n'est pas toujours possible de faire les inoculations à ce stade, ainsi des inoculations sont parfois faites quelques jours avant ou après la mi-anthèse. L'objectif de cette étude était de déterminer si l'inoculation, par la méthode du vide partiel, d'épis de blé avec l’U. tritici à différents stades du développement de l'épi pouvait avoir un effet sur le nombre de grains viables par épi et, ultérieurement, sur le pourcentage de plantes cariées issues de grains inoculés. À chaque année durant 5 ans, quatre lignées de blé ont été inoculées au champ à trois différents stades du développement de l'épi. Les stades du développement de l'épi étudiés étaient la pré-anthèse, la mi-anthèse (les anthères à chaque extrémité de l'épi sont déhiscentes alors que celles du centre sont jaunes) et la post-anthèse (toutes les anthères sont déhiscentes). Il y avait des différences significatives entre les quatre lignées de blé et les 5 années de l'étude quant au nombre de grains viables par épi et le pourcentage de plantes cariées. Un nombre de grains viables par épi plus grand et un pourcentage plus faible de plantes cariées ont été significativement obtenus avec l'inoculation post-anthèse. Nous concluons que l'inoculation de l’Ustilago tritici par la méthode du vide partiel doit être faite au stade préanthèse ou mi-anthèse du développement de l'épi de blé afin de minimiser les effets négatifs de la maturité sur l'efficacité d'infection.In general, in western Canada, the inoculation of wheat (Triticum aestivum) lines to determine the level of resistance to Ustiiago tritici occurs when the plants are at the mid-anthesis stage of spike development. However, it is not always possible to inoculate at this stage so occasionally inoculations are done a few days before or after mid-anthesis. The objective of this study was to determine if inoculation of wheat spikes with U. tritici at different stages of spike development using the partial-vacuum method affected the number of viable seed per spike and subsequent percentage of smutted plants grown from inoculated seed. Four lines of wheat were inoculated at three different stages of spike development in the field each year for 5 years. The stages of spike development studied were preanthesis, mid-anthesis (anthers at either end of the spike were dehisced while those in the middle of the spike were yellow), and post-anthesis (all anthers dehisced). There were significant differences among the four wheat lines and the 5 years of the study for the number of viable seed per spike and the percent of smutted plants. Inoculation at post-anthesis resulted in a significantly greater number of viable seed per spike and lower percentage of smutted plants. We conclude that the partial-vacuum method of inoculation with Ustiiago tritici should be done at the pre- or midanthesis stage of wheat head development to minimize the negative effect of maturity on infection efficiency

Ergot: a perennial issue?

Non-Peer Reviewe

A resistance gene to Ustilago nuda in barley is located on chromosome 3H

Canadian Journal of Plant Pathology website: http://www.tandfonline.com/loi/tcjp20Loose smut of barley is a common disease which can be controlled using resistant varieties. Information on the chromosome location of loci controlling loose smut resistance and the development of molecular markers to aid in selection for these genes can be beneficial in the resistant variety development process. The objectives of this work were to determine the resistance or susceptibility of doubled haploid barley lines arising from a cross of the varieties ‘Steptoe’ and ‘Morex’ to Ustilago nuda, the causal agent of loose smut of barley, and map the chromosome location of the loose smut resistance locus in ‘Morex’. The reaction to Ustilago nuda of the doubled-haploid barley plants was determined by inoculating spikelets of each line at anthesis by injection of a teliospore suspension using a needle inoculation method. Mature seeds from the inoculated spikelets were grown to determine the percentage of plants that developed with smutted heads. The lines were classified as susceptible if greater than 10% of the plants were smutted. The loose smut resistance locus from the resistant source ‘Morex’ was mapped using an existing DNA marker map of the ‘Steptoe’/‘Morex’ population. The distribution of the resistant and susceptible progeny from the loose smut testing fit a single gene model. The resistance gene was mapped to chromosome 3 (3H)

Influence of gestational age at initiation of antihypertensive therapy: secondary analysis of CHIPS trial data (control of hypertension in pregnancy study)

For hypertensive women in CHIPS (Control of Hypertension in Pregnancy Study), we assessed whether the maternal benefits of tight control could be achieved, while minimizing any potentially negative effect on fetal growth, by delaying initiation of antihypertensive therapy until later in pregnancy. For the 981 women with nonsevere, chronic or gestational hypertension randomized to less-tight (target diastolic blood pressure, 100 mm Hg), or tight (target, 85 mm Hg) control, we used mixed-effects logistic regression to examine whether the effect of less-tight (versus tight) control on major outcomes was dependent on gestational age at randomization, adjusting for baseline factors as in the primary analysis and including an interaction term between gestational age at randomization and treatment allocation. Gestational age was considered categorically (quartiles) and continuously (linear or quadratic form), and the optimal functional form selected to provide the best fit to the data based on the Akaike information criterion. Randomization before (but not after) 24 weeks to less-tight (versus tight) control was associated with fewer babies with birth weight 48 hours (Pinteraction=0.354). For the mother, less-tight (versus tight) control was associated with more severe hypertension at all gestational ages but particularly so before 28 weeks (Pinteraction=0.076). In women with nonsevere, chronic, or gestational hypertension, there seems to be no gestational age at which less-tight (versus tight) control is the preferred management strategy to optimize maternal or perinatal outcomes

The Cholecystectomy As A Day Case (CAAD) Score: A Validated Score of Preoperative Predictors of Successful Day-Case Cholecystectomy Using the CholeS Data Set

Background Day-case surgery is associated with significant patient and cost benefits. However, only 43% of cholecystectomy patients are discharged home the same day. One hypothesis is day-case cholecystectomy rates, defined as patients discharged the same day as their operation, may be improved by better assessment of patients using standard preoperative variables. Methods Data were extracted from a prospectively collected data set of cholecystectomy patients from 166 UK and Irish hospitals (CholeS). Cholecystectomies performed as elective procedures were divided into main (75%) and validation (25%) data sets. Preoperative predictors were identified, and a risk score of failed day case was devised using multivariate logistic regression. Receiver operating curve analysis was used to validate the score in the validation data set. Results Of the 7426 elective cholecystectomies performed, 49% of these were discharged home the same day. Same-day discharge following cholecystectomy was less likely with older patients (OR 0.18, 95% CI 0.15–0.23), higher ASA scores (OR 0.19, 95% CI 0.15–0.23), complicated cholelithiasis (OR 0.38, 95% CI 0.31 to 0.48), male gender (OR 0.66, 95% CI 0.58–0.74), previous acute gallstone-related admissions (OR 0.54, 95% CI 0.48–0.60) and preoperative endoscopic intervention (OR 0.40, 95% CI 0.34–0.47). The CAAD score was developed using these variables. When applied to the validation subgroup, a CAAD score of ≤5 was associated with 80.8% successful day-case cholecystectomy compared with 19.2% associated with a CAAD score >5 (p < 0.001). Conclusions The CAAD score which utilises data readily available from clinic letters and electronic sources can predict same-day discharges following cholecystectomy