The diary project

Negative body perception is both a personal and social stigma that is felt by many women, regardless of their cultural background. Design can be a tool to help women reflect on their journey with their bodies and start a conversation about this problem. This thesis is attempting to work on a small aspect of negative body perception by working on the self and integrating women in the process of the project. This thesis began as a self-reflective approach to body image. It evolved into a participatory project where women utilized self-communication based on a design probe. The aim of the project is to design a communication tool that enables women to have a positive relationship with their bodies. Background research provides knowledge on the topic of body perception and how design is contributing to positive body image. Empirical research begins in the exploration of the topic of body image through a survey and an exhibition project and continues with interviews of ten women from different cultural backgrounds to discuss the pressures they felt connected to their body perception. Data collection and self-reflection leads to the design of a diary probe that is distributed for the women to fill in. The interpretation and analysis of the diaries, as well as the feedback provided, shows that these women benefited from this exploration of themselves and the project achieved fruitful results. This diary is a starting point for someone to get to know themselves and focus on growing their body acceptance. It can also be of aid to young people, and those who want to renew their relationship with their bodies or begin a better one. It is a safe and relaxed way to venture down the road of self-discovery and acceptance

Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco

Post-weaning diarrhea (PWD) in piglets is a major problem in piggeries worldwide and results in severe economic losses. Infection with Enterotoxigenic Escherichia coli (ETEC) is the key culprit for the PWD disease. F4 fimbriae of ETEC are highly stable proteinaceous polymers, mainly composed of the major structural subunit FaeG, with a capacity to evoke mucosal immune responses, thus demonstrating a potential to act as an oral vaccine against ETEC-induced porcine PWD. In this study we used a transplastomic approach in tobacco to produce a recombinant variant of the FaeG protein, rFaeG(ntd/dsc), engineered for expression as a stable monomer by N-terminal deletion and donor strand-complementation (ntd/dsc). The generated transplastomic tobacco plants accumulated up to 2.0 g rFaeG(ntd/dsc) per 1 kg fresh leaf tissue (more than 1% of dry leaf tissue) and showed normal phenotype indistinguishable from wild type untransformed plants. We determined that chloroplast-produced rFaeG(ntd/dsc) protein retained the key properties of an oral vaccine, i.e. binding to porcine intestinal F4 receptors (F4R), and inhibition of the F4-possessing (F4+) ETEC attachment to F4R. Additionally, the plant biomass matrix was shown to delay degradation of the chloroplast-produced rFaeG(ntd/dsc) in gastrointestinal conditions, demonstrating a potential to function as a shelter-vehicle for vaccine delivery. These results suggest that transplastomic plants expressing the rFaeG(ntd/dsc) protein could be used for production and, possibly, delivery of an oral vaccine against porcine F4+ ETEC infections. Our findings therefore present a feasible approach for developing an oral vaccination strategy against porcine PWD

Effect of Systemic Diseases on Periodontal Microbiome. A Literature Review. Part III

Periodontitis is defined as a chronic inflammatory disease and is mainly caused by a dysbiosis of the periodontal microbiome. Many systemic diseases have been linked to periodontal disease, and the alteration of the microbiome plays a major role in the pathogenesis. Diabetes has been highly associated with the increased risk of periodontal disease, as it provides a hyperglycemic microenvironment that heavily influences the periodontal microbiome by reducing its diversity and favoring disease associated bacteria. Rheumatoid arthritis has also been associated with periodontitis, with many studies indicating microbial shifts in affected individuals without reaching a consensus on the precise nature of dysbiosis. Contradictory and limited number of studies focusing on the effect of other diseases (systemic lupus erythematosus, human immunodeficiency virus, leucocyte adhesion deficiency, liver diseases) on the periodontal microbiome have been also conducted, and many of them have shown distinct microbial shifts in affected individuals

Drug induced gingival hyperplasia

Thesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 1998 (Periodontology and Oral Biology).Includes bibliographical references: (leaves 31-35).Drug induced gingival hyperplasia is a side effect of certain drugs taken systemically which have an effect intraorally and can affect function and esthetics. In this study we tried to find an explanation at the molecular level. We know from previous experiments that lysyl oxidase is responsible for the cross-linking of collagen. Basic fibroblast growth factor down regulates mRNA levels of lysyl oxidase. The goal of this study was to determine whether drugs (cyclosporin A, dilantin, and nifedipine) block this down regulation allowing increased deposition of ground substance in the extracellular matrix leading to fibrosis. Gingival fibroblasts were treated with nifedipine plus or minus basic fibroblast growth factor. RNA was isolated and changes in lysyl oxidase, collagen, and elastin mRNA levels were measured. The lysyl oxidase enzyme activity was also measured to see if it was concordant with the mRNA levels. Down regulation by basic fibroblast growth factor was reproducible at high concentrations of basic fibroblast growth factor only (1 nM). At 0.1 nM this down regulation was not consistant anymore. The blocking effect produced by the drugs was not reproducible at high or low concentrations of basic growth factor. This may be due to the stage at which the cells were treated, the kind of cells, or the time of treatment

Valuing diversity : an examination of diversity climate, work attitudes, and employee racioethnicities

This study examines the relations between diversity climate, characterized by organizational fairness and organizational inclusion, and employees' work attitudes (i.e., job satisfaction and affective, normative and continuance commitment). It was proposed that the degree of perceived organizational support would mediate the relationships between diversity climate and employees' work attitudes (i.e., job satisfaction, affective commitment and normative commitment). Furthermore, employees' racioethnicities were hypothesized to moderate the relationships between diversity climate and work attitudes. Employees (N = 311) in organizations in Canada completed a self-report survey regarding their perceptions on the organization's diversity climate and organizational support, and their levels of job satisfaction and organizational commitment. Regression analyses revealed that diversity climate is positively related to job satisfaction, affective and normative commitment, and that perceived organizational support mediates the relationship between diversity climate and these employee attitudes. Contrary to what had been hypothesized, diversity climate is negatively associated with continuance commitment. Furthermore, racioethnicity generally did not moderate the relations between diversity climate and work attitudes

Les attentes, la satisfaction et la qualité de vie des édentés suite à une réhabilitation par mise en charge immédiate d'une prothèse totale reliée à deux implants non jumelés : une étude pilote expérimentale

Problématique : La majorité des études publiées sur la réhabilitation par mise en charge immédiate de deux implants non jumelés avec une prothèse totale mandibulaire de recouvrement n’ont rapporté que des mesures cliniques objectives et très peu ont évalué les mesures centrées sur le patient, et ce, avec des erreurs de mesure. Aucune étude n’a évalué les attentes des patients vis-à-vis d'un tel protocole. Objectifs : Évaluer les attentes, le niveau de satisfaction ainsi que la qualité de vie reliée à la santé bucco-dentaire des édentés complets suite à un protocole de mise en charge immédiate. Méthodologie : Cet essai clinique de phase 1 utilise un design pré-post afin d’évaluer les mesures centrées sur le patient. Dix-huit individus, complètement édentés et âgés en moyenne de 62,39 ± 7,65 ans, ont reçu une prothèse totale mandibulaire de recouvrement sur deux implants non jumelés suite à un protocole de mise en charge immédiate, conjointement à une prothèse totale conventionnelle maxillaire. Un instrument adapté pour mesurer leurs attentes à l’aide d’échelles visuelles analogues, le questionnaire « McGill Denture Satisfaction Instrument » ainsi que le questionnaire OHIP-20 ont été remis aux patients avant de procéder aux traitements (T0), ainsi qu’aux rendez-vous de suivi à 2 semaines (T1), 1 mois (T2) et 4 mois (T3). De plus, l’inventaire de personnalité révisé (NÉO PI-R) ainsi qu’un questionnaire sociodémographique ont été remplis par les participants. Les « change scores » ont été calculés puis des tests non paramétriques et des analyses de variances en mesures répétées suivies de comparaisons par paires ont été utilisés afin d’analyser les données recueillies. La taille d’effet a été estimée. Résultats : Les participants avaient différentes attentes par rapport à la mise en charge immédiate. Certains s’attendaient à un effet positif à court terme par rapport à leur apparence esthétique (83,3 %) et à leur vie sociale (55,7 %), alors que d’autres avaient des craintes envers leur confort (5,6 %), leur habileté à mastiquer (11,1 %) et à nettoyer leur prothèse inférieure (11,1 %). À 4 mois, le protocole de mise en charge immédiate avait rencontré la majorité des attentes des patients par rapport à l’esthétique (94.4 %), la mastication (83.3 %), la phonétique (61.1 %), le confort (94.4 %), l’hygiène (88.9 %) et leur vie sociale (88.9 %). Une amélioration statistiquement significative de la satisfaction en générale, du confort, de l’esthétique, de la stabilité de la prothèse inférieure et de l’habileté à mastiquer a été notée à 2 semaines (p<0,001). Également, les comparaisons par paires ont révélé une diminution statistiquement significative du score total de l’OHIP-20 (p < 0,001) de même que la majorité des domaines de l’OHIP (p < 0.01), sauf pour l’handicap social qui n’a diminué significativement qu’après 1 mois (p = 0.01). Ces changements (pour la satisfaction et la qualité de vie) sont restés stables au cours des suivis subséquents. Indépendamment des traits de personnalité et des variables sociodémographiques, le protocole immédiat a satisfait 94,4 % des participants et a amélioré leur qualité de vie avec une large magnitude d’effet (d = 1.9; p < 0.001). Bien que deux patients aient perdu des implants au cours du traitement, 100 % des participants étaient d’accord pour recommander cette procédure à leurs pairs. Conclusions: Le protocole de mise en charge immédiate semble satisfaire les patients quelles que soient leurs attentes. Le protocole MCI peut améliorer, à court terme, la qualité de vie des patients totalement édentés. Les résultats prometteurs de la phase 1 devraient être corroborés lors de la 2e phase de cette étude.Statement of problem: Most published research on the immediate loading of a mandibular 2-implant overdenture has only reported on clinical outcomes. Few studies have evaluated patients centered-outcomes but with measurement errors. Consequently, there is a need to assess clinical and patient-reported outcomes in this regard. Objectives: To examine patients’ level of satisfaction, expectations and quality of life with regard to the implants’ immediate loading protocol in edentate individuals. Methods: This phase-I trial used a pre-post design to assess patient-centered outcomes in 18 edentate individuals (mean age 62.39 ± 7.65 years) who have received a 2-implant (unsplinted) mandibular overdenture through an immediate loading protocol. The McGill Denture Satisfaction Instrument, a VAS instrument assessing expectations and the OHIP-20 questionnaire were used to evaluate patients’ outcomes at baseline, 2 weeks, 1 and 4 months. Socio-demographic and personality trait data were obtained using the Revised NEO Personality Inventory and a self-administered questionnaire. Change scores were calculated and non-parametrical tests were used to analyse the data. Repeated measures ANOVAs and paired t-tests were also conducted, and effect size was estimated. Results: The participants had a wide range of expectations regarding the immediate loading protocol. Expectations included short-term positive impact on aesthetics (83.3 %) and social life (55.7 %). Other expectations included negative effects on comfort (5.6%), the ability to chew (11.1 %) and to clean their lower denture (11.1%). At 4 months, the immediate loading protocol had met most patients’ expectations regarding aesthetics (94.4%), the ability to chew (83.3%), ability to speak (61.1%), comfort (94.4%), the ability to clean their lower denture (88.9%) and their social life (88.9%). The statistical analysis showed an overall satisfaction increase with a median change score of 66 (range=7-98), 68 (range=7- 95), and 70 (range= 17- 99), at 2 weeks, 1 month and 4 months respectively. There was a statistically significant improvement in overall satisfaction, comfort, perceived aesthetics, stability of the overdenture, and ability to chew at 2 weeks (p < 0,001). Pairwise comparison (within-subjects comparisons) revealed significant decreases in total OHIP scores (P < 0.001), as well as fewer functional limitations, physical pain, psychological discomfort, physical disability, psychological disability, and handicap (P < 0.01) from baseline to 2 weeks, 1 month and 4 months follow-up. Social disability score decrease significantly only after 1 month follow-up (p = 0.01). These changes remained stable during subsequent follow-ups. The protocol satisfied 94.4% of the participants and improve their quality of life with an estimated effect size of 1.9 (p < 0.001), regardless of socio-demographic and personality profiles. There was 100% agreement among patients on recommending this procedure to their peers. Conclusions: Immediate loading of two unsplinted implants with a mandibular overdenture seemed to fulfill patients’ satisfaction regardless of their expectations and may improve oral health-related quality life of edentate individuals in short-term. Encouraging results from phase I should be confirmed and approved in Phase II clinical trials

Method of Improving Weld Quality

A method of improving weld quality between aluminum members by slowing the rate of solidification of a molten weld trough into solidified material. Upper and lower aluminum members are positioned together in contact between facing surfaces thereof to expose a first outer surface of the upper aluminum member to laser irradiation. A welding laser beam is moved in a path over the first outer surface, wherein the welding laser beam has an energy and width to progressively melt a trough of molten metal to a depth through the upper aluminum member and into the lower aluminum member. The molten metal in the trough has a void filled with gas, and the molten metal re-solidifies into re-solidified metal after the passage of the welding laser beam. An area in and around the trough is heated to slow the rate of solidification of the molten metal into the re-solidified metal, thereby preventing entrainment of the gas within the re-solidified metal

The deleterious effect of crossfostering in rat pups on hypoxic-ischemic injury tolerance and hypothermic neuroprotection

We study the effect of hypothermia (HT) following hypoxic-ischemic (HI) brain injury in postnatal day 7 (P7) rats. In 2015, new European Union animal transport regulations prompted a change in practice at the breeding facility, which henceforth crossfostered P3 litters to P8 older lactating dam prior to transportation. It is generally assumed that crossfostering does not significantly affect the experimental results. The aim of this study was to examine whether crossfostering affects our model consistency by modifying injury susceptibility and hypothermic neuroprotection. We analysed 219 pups (56 litters) from 11 experiments conducted between 2013 and 2015: 73 non-crossfostered and 146 crossfostered pups. At P7, all pups underwent unilateral common carotid artery ligation followed by 50min of hypoxia (8% O2, 36°C). Immediately after this mild insult, the pups were randomised to post-insult normothermia (NT) or HT treatment. Pups were culled at P14. Injury was assessed by area loss of the ipsilateral hemisphere and histopathology scoring of hippocampus, cortex, thalamus, and basal ganglia. Crossfostered pups had double the injury compared to non-crossfostered pups irrespective of treatment group. Hypothermic neuroprotection was statistically significant, but with a smaller and less consistent effect in crossfostered pups (relative neuroprotection 16% vs. 31% in non-crossfostered). These results demonstrate hypothermic neuroprotection following a mild HI insult. A representative subset of 41 animals were also assessed for evidence of microglial reactivity, however no detectable difference in microglial reactivity was observed between any of the groups. In conclusion, crossfostering alters outcomes in our established model through reduced insult tolerance and variable neuroprotection. Crossfostering as a common breeding practice is a largely unexplored variable in animal research that may result in invalid research conclusions if inadequately adjusted for by larger group sizes. As a result, crossfostering is likely to be inconsistent with the principles of replacement, reduction, and refinement