Measuring soft measures within a stated preference survey: The effect of pollution and traffic stress on mode choice

The objective of this research is to study the extent to which information on pollution and individual stress has on the choice to shift from private car to Park and Ride. A Stated Preference experiment was built where the reduction of CO2 and stress are attributes of the experimental design. Results showed that the utility to Park and Ride increases with the level of awareness, 2) the more individuals consider receiving information about stress useful, the more they tend to behave sustainably, 3) aspects associated with stress appear to have a greater influence on travel choice than environmental aspects

A hybrid discrete choice model to assess the effect of awareness and attitude towards environmentally friendly travel modes

The need to reduce private vehicle use has led to the development of soft measures aimed at re-educating car users through information processes that raise their awareness regarding the benefits of environmentally friendly modes, encouraging them to voluntarily change their mode choice behaviour (level-of-service characteristics being equal). It has been observed, though not scientifically demonstrated, that these measures can produce changes, being the result of mindful decisions. However, in some cases, as demonstrated by numerous environmental psychology studies such measures are not sufficient to overcome the effect of cognitive dissonance, one of the main factors hindering change. In fact it is not unusual to find discrepancies between attitudes and behaviour in travel behaviour research. The objective of the present work is to understand the relationship between awareness, attitude and behaviour in the context of mode choice and to measure the effect of awareness after the implementation of a soft measure after controlling for individual environmental attitudes. Using a dataset gathered in two weeks, before and after individuals are informed of the benefits of using park and ride (P&R) instead of their car, we estimated a hybrid mode choice model

Development of a Technological Platform for Implementing VTBC Programs

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CDL: CILEA Digital Library. "Accesso alle risorse elettroniche: esperienze e prospettive tratte dall'analisi delle statistiche"

CILEA, contributing to the creation of an italian digital library, created the CDL (CILEA Digital Libray service)

CDL: CILEA Digital Library. "Accesso alle risorse elettroniche: esperienze e prospettive tratte dall'analisi delle statistiche"

CILEA, contributing to the creation of an italian digital library, created the CDL (CILEA Digital Libray service)

Testicular degeneration and infertility following arbovirus infection

Arboviruses can cause a variety of clinical signs including febrile illness, arthritis, encephalitis and hemorrhagic fever. The recent Zika epidemic highlighted the possibility that arboviruses may also negatively affect the male reproductive tract. In this study, we focused on bluetongue virus (BTV), the causative agent of bluetongue and one of the major arboviruses of ruminants. We show that rams that recovered from bluetongue displayed signs of testicular degeneration and azoospermia up to 100 days after the initial infection. Importantly, testicular degeneration was induced in rams experimentally infected with either a high (BTV-1IT2006) or low (BTV-1IT2013) virulence strain of BTV. Rams infected with the low virulent BTV strain displayed testicular lesions in the absence of other major clinical signs. Testicular lesions in BTV-infected rams were due to viral replication in the endothelial cells of the peritubular areas of the testes, resulting in stimulation of a type-I IFN response, reduction of testosterone biosynthesis by Leydig cells, and destruction of Sertoli cells and the blood-testis barrier in more severe cases. Hence, BTV induces testicular degeneration and disruption of spermatogenesis by replicating solely in the endothelial cells of the peritubular areas unlike other gonadotropic viruses. This study shows that a naturally occurring arboviral disease can cause testicular degeneration and affect male fertility at least temporarily

Propensity for Voluntary Travel Behavior Changes: An Experimental Analysis

In this paper we analyze individual propensity to voluntary travel behavior change combining concepts from theory of change with the methodologies deriving from behavioral models. In particular, following the theory of voluntary changes, we set up a two-week panel survey including soft measure implementation, which consisted of providing car users with a personalized travel plan after the first week of observation (before) and using the second week to monitoring the post-behavior (after). These data have then been used to estimate a Mixed Logit for the choice to use a personal vehicle or a light metro; and a Multinomial Logit for the decision to change behavior. Results from both models show the relevance of providing information about available alternatives to individuals while promoting voluntary travel behavioral change

A proof-of-concept analysis of plasma-derived exosomal microRNAs in interstitial pulmonary fibrosis secondary to antisynthetase syndrome

Antisynthetase syndrome (ASSD) is an autoimmune disease characterized by the positivity of autoantibodies against different aminoacyl transfer RNA (tRNA) synthetases. Morbidity and mortality of this disease are highly affected by interstitial lung disease (ILD) which is present in about 80% of patients. In this study, we investigated possible differences in 84 immune-related circulating miRNAs between ASSD patients with and without ILD; we enrolled 15 ASSD patients, 11 with ILD (ILD+) and 4 without ILD (ILD-), and 5 patients with idiopathic pulmonary fibrosis (IPF) as an additional control group. All patients were at disease onset and not on therapy at the time of inclusion. Differentially expressed miRNAs were identified in plasma-derived exosomes, using an miRNA PCR array (MIHS-111ZG, Qiagen, Hilden, Germany); miR-30a-5p and miR-29c-3p were upregulated in ASSD-ILD patients compared to patients without lung involvement (adjusted p-value < 0.05). IPF patients showed higher miR-29c-3p expression levels with respect to both ASSD and ASSD-ILD (p = 0.0005), whereas levels of miR-30a-5p were not different. miR-29c-3p and miR-30a-5p are overexpressed in ASSD-ILD+ patients compared with ILD?. These miRNAs are involved in the regulation of inflammation and fibrosis through their action on NF-?B and TGF-?1. Although the mechanistic role of these miRNAs in ASSD-ILD development has to be elucidated, we suggest that their exosome levels could be useful in identifying patients at risk of ILD.Funding: This research was supported by the Ministry of Health IRCCS Foundation Policlinico San Matteo Grant [grant number 948-rcr2019i2-46]

Vitamin D responsive elements within the HLA-DRB1 promoter region in Sardinian multiple sclerosis associated alleles

Vitamin D response elements (VDREs) have been found in the promoter region of the MS-associated allele HLA-DRB1*15:01, suggesting that with low vitamin D availability VDREs are incapable of inducing *15:01 expression allowing in early life autoreactive T-cells to escape central thymic deletion. The Italian island of Sardinia exhibits a very high frequency of MS and high solar radiation exposure. We test the contribution of VDREs analysing the promoter region of the MS-associated DRB1 *04:05, *03:01, *13:01 and *15:01 and non-MS-associated *16:01, *01, *11, *07:01 alleles in a cohort of Sardinians (44 MS patients and 112 healthy subjects). Sequencing of the DRB1 promoter region revealed a homozygous canonical VDRE in all *15:01, *16:01, *11 and in 45/73 *03:01 and in heterozygous state in 28/73 *03:01 and all *01 alleles. A new mutated homozygous VDRE was found in all *13:03, *04:05 and *07:01 alleles. Functionality of mutated and canonical VDREs was assessed for its potential to modulate levels of DRB1 gene expression using an in vitro transactivation assay after stimulation with active vitamin D metabolite. Vitamin D failed to increase promoter activity of the *04:05 and *03:01 alleles carrying the new mutated VDRE, while the *16:01 and *03:01 alleles carrying the canonical VDRE sequence showed significantly increased transcriptional activity. The ability of VDR to bind the mutant VDRE in the DRB1 promoter was evaluated by EMSA. Efficient binding of VDR to the VDRE sequence found in the *16:01 and in the *15:01 allele reduced electrophoretic mobility when either an anti-VDR or an anti-RXR monoclonal antibody was added. Conversely, the Sardinian mutated VDRE sample showed very low affinity for the RXR/VDR heterodimer. These data seem to exclude a role of VDREs in the promoter region of the DRB1 gene in susceptibility to MS carried by DRB1* alleles in Sardinian patients