310 research outputs found
La pintura ilusionista entre Europa y América
Aborda el libro la pintura decorativa y su conexión con la perspectiva arquitectónica en el mundo iberoamericano. Desde las experiencias italianas, con un gran desarrollo de la "quadratura" como soporte de este arte decorativo, hasta conocer su proyección en el mundo americano, donde Brasil fue especialmente receptivo a estas experiencias en que se aunaron arte y ciencia.Área de Historia del Arte, UPOVersión del edito
Identification and analysis of vocal communication pathways in birds through inducible gene expression
WFPC2 Observations of the Hubble Deep Field-South
The Hubble Deep Field-South observations targeted a high-galactic-latitude
field near QSO J2233-606. We present WFPC2 observations of the field in four
wide bandpasses centered at roughly 300, 450, 606, and 814 nm. Observations,
data reduction procedures, and noise properties of the final images are
discussed in detail. A catalog of sources is presented, and the number counts
and color distributions of the galaxies are compared to a new catalog of the
HDF-N that has been constructed in an identical manner. The two fields are
qualitatively similar, with the galaxy number counts for the two fields
agreeing to within 20%. The HDF-S has more candidate Lyman-break galaxies at z
> 2 than the HDF-N. The star-formation rate per unit volume computed from the
HDF-S, based on the UV luminosity of high-redshift candidates, is a factor of
1.9 higher than from the HDF-N at z ~ 2.7, and a factor of 1.3 higher at z ~ 4.Comment: 93 pages, 25 figures; contains very long table
Quantum Graphs: A simple model for Chaotic Scattering
We connect quantum graphs with infinite leads, and turn them to scattering
systems. We show that they display all the features which characterize quantum
scattering systems with an underlying classical chaotic dynamics: typical
poles, delay time and conductance distributions, Ericson fluctuations, and when
considered statistically, the ensemble of scattering matrices reproduce quite
well the predictions of appropriately defined Random Matrix ensembles. The
underlying classical dynamics can be defined, and it provides important
parameters which are needed for the quantum theory. In particular, we derive
exact expressions for the scattering matrix, and an exact trace formula for the
density of resonances, in terms of classical orbits, analogous to the
semiclassical theory of chaotic scattering. We use this in order to investigate
the origin of the connection between Random Matrix Theory and the underlying
classical chaotic dynamics. Being an exact theory, and due to its relative
simplicity, it offers new insights into this problem which is at the fore-front
of the research in chaotic scattering and related fields.Comment: 28 pages, 13 figures, submitted to J. Phys. A Special Issue -- Random
Matrix Theor
The Hubble Deep Field South Flanking Fields
As part of the Hubble Deep Field South program, a set of shorter 2-orbit
observations were obtained of the area adjacent to the deep fields. The WFPC2
flanking fields cover a contiguous solid angle of 48 square arcminutes.
Parallel observations with the STIS and NICMOS instruments produce a patchwork
of additional fields with optical and near-infrared (1.6 micron) response.
Deeper parallel exposures with WFPC2 and NICMOS were obtained when STIS
observed the NICMOS deep field. These deeper fields are offset from the rest,
and an extended low surface brightness object is visible in the deeper WFPC2
flanking field. In this data paper, which serves as an archival record of the
project, we discuss the observations and data reduction, and present SExtractor
source catalogs and number counts derived from the data. Number counts are
broadly consistent with previous surveys from both ground and space. Among
other things, these flanking field observations are useful for defining slit
masks for spectroscopic follow-up over a wider area around the deep fields, for
studying large-scale structure that extends beyond the deep fields, for future
supernova searches, and for number counts and morphological studies, but their
ultimate utility will be defined by the astronomical community.Comment: 46 pages, 15 figures. Images and full catalogs available via the
HDF-S at http://www.stsci.edu/ftp/science/hdfsouth/hdfs.html at present. The
paper is accepted for the February 2003 Astronomical Journal. Full versions
of the catalogs will also be available on-line from AJ after publicatio
Noradrenergic Control of Gene Expression and Long-Term Neuronal Adaptation Evoked by Learned Vocalizations in Songbirds
Norepinephrine (NE) is thought to play important roles in the consolidation and retrieval of long-term memories, but its role in the processing and memorization of complex acoustic signals used for vocal communication has yet to be determined. We have used a combination of gene expression analysis, electrophysiological recordings and pharmacological manipulations in zebra finches to examine the role of noradrenergic transmission in the brain’s response to birdsong, a learned vocal behavior that shares important features with human speech. We show that noradrenergic transmission is required for both the expression of activity-dependent genes and the long-term maintenance of stimulus-specific electrophysiological adaptation that are induced in central auditory neurons by stimulation with birdsong. Specifically, we show that the caudomedial nidopallium (NCM), an area directly involved in the auditory processing and memorization of birdsong, receives strong noradrenergic innervation. Song-responsive neurons in this area express α-adrenergic receptors and are in close proximity to noradrenergic terminals. We further show that local α-adrenergic antagonism interferes with song-induced gene expression, without affecting spontaneous or evoked electrophysiological activity, thus dissociating the molecular and electrophysiological responses to song. Moreover, α-adrenergic antagonism disrupts the maintenance but not the acquisition of the adapted physiological state. We suggest that the noradrenergic system regulates long-term changes in song-responsive neurons by modulating the gene expression response that is associated with the electrophysiological activation triggered by song. We also suggest that this mechanism may be an important contributor to long-term auditory memories of learned vocalizations
Neural Correlates of Threat Perception: Neural Equivalence of Conspecific and Heterospecific Mobbing Calls Is Learned
Songbird auditory areas (i.e., CMM and NCM) are preferentially activated to playback of conspecific vocalizations relative to heterospecific and arbitrary noise [1]–[2]. Here, we asked if the neural response to auditory stimulation is not simply preferential for conspecific vocalizations but also for the information conveyed by the vocalization. Black-capped chickadees use their chick-a-dee mobbing call to recruit conspecifics and other avian species to mob perched predators [3]. Mobbing calls produced in response to smaller, higher-threat predators contain more “D” notes compared to those produced in response to larger, lower-threat predators and thus convey the degree of threat of predators [4]. We specifically asked whether the neural response varies with the degree of threat conveyed by the mobbing calls of chickadees and whether the neural response is the same for actual predator calls that correspond to the degree of threat of the chickadee mobbing calls. Our results demonstrate that, as degree of threat increases in conspecific chickadee mobbing calls, there is a corresponding increase in immediate early gene (IEG) expression in telencephalic auditory areas. We also demonstrate that as the degree of threat increases for the heterospecific predator, there is a corresponding increase in IEG expression in the auditory areas. Furthermore, there was no significant difference in the amount IEG expression between conspecific mobbing calls or heterospecific predator calls that were the same degree of threat. In a second experiment, using hand-reared chickadees without predator experience, we found more IEG expression in response to mobbing calls than corresponding predator calls, indicating that degree of threat is learned. Our results demonstrate that degree of threat corresponds to neural activity in the auditory areas and that threat can be conveyed by different species signals and that these signals must be learned
A Potential Neural Substrate for Processing Functional Classes of Complex Acoustic Signals
Categorization is essential to all cognitive processes, but identifying the neural substrates underlying categorization processes is a real challenge. Among animals that have been shown to be able of categorization, songbirds are particularly interesting because they provide researchers with clear examples of categories of acoustic signals allowing different levels of recognition, and they possess a system of specialized brain structures found only in birds that learn to sing: the song system. Moreover, an avian brain nucleus that is analogous to the mammalian secondary auditory cortex (the caudo-medial nidopallium, or NCM) has recently emerged as a plausible site for sensory representation of birdsong, and appears as a well positioned brain region for categorization of songs. Hence, we tested responses in this non-primary, associative area to clear and distinct classes of songs with different functions and social values, and for a possible correspondence between these responses and the functional aspects of songs, in a highly social songbird species: the European starling. Our results clearly show differential neuronal responses to the ethologically defined classes of songs, both in the number of neurons responding, and in the response magnitude of these neurons. Most importantly, these differential responses corresponded to the functional classes of songs, with increasing activation from non-specific to species-specific and from species-specific to individual-specific sounds. These data therefore suggest a potential neural substrate for sorting natural communication signals into categories, and for individual vocal recognition of same-species members. Given the many parallels that exist between birdsong and speech, these results may contribute to a better understanding of the neural bases of speech
Nucleofection induces non-specific changes in the metabolic activity of transfected cells
Transfection has become an everyday technique widely used for functional studies in living cells. The choice of the particular transfection method is usually determined by its efficiency and toxicity, and possible functional consequences specific to the method used are normally overlooked. We describe here that nucleofection, a method increasingly used because of its convenience and high efficiency, increases the metabolic rate of some cancer cells, which can be misleading when used as a measure of proliferation. Moreover, nucleofection can alter the subcellular expression pattern of the transfected protein. These undesired effects are independent of the transfected nucleic acid, but depend on the particular cell line used. Therefore, the interpretation of functional data using this technology requires further controls and caution
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