Identification and GroEL gene characterization of green petal phytoplasma infecting strawberry in Italy

none5The presence of phytoplasmas in strawberry showing malformation of the fruits together with the typical green petals symptoms was detected in some North Western Italy cultivations. Nucleic acids extracted from these plants were used in nested-PCR assays with primers amplifying 16S rDNA and GroEL sequences specific for phytoplasmas. Bands of 1.2 kb were obtained in both cases after nested-PCR assays and RFLP analyses allowed to classify the detected phytoplasmas in the aster yellows subgroup 16SrI-C, the GroELI grouping confirm all the strains from strawberry to be identical to each other and to GroELI-VI group. This is the first multigene molecular identification of strawberry green petals phytoplasmas in Italy.openContaldo N.; J.F. Mejia; S. Paltrinieri; A. Calari; A. BertacciniContaldo N.; J.F. Mejia; S. Paltrinieri; A. Calari; A. Bertaccin

Compress sensing algorithm for estimation of signals in sensor networks

ARTICULO DE INVESTIGACION IDEXADO EN JCR CON FACTOR DE IMPACTO 2.4In this research, we present a data recovery scheme for wireless sensor networks. In some sensor networks, each node must be able to recover the complete information of the network, which leads to the problem of the high cost of energy in communication and storage of information. We proposed a modified gossip algorithm for acquire distributed measurements and communicate the information across all nodes of the network using compressive sampling and Gossip algorithms to compact the data to be stored and transmitted through a network. The experimental results on synthetic data show that the proposed method reconstruct better the signal and in less iterations than with a similar method using a thresholding algorithm

Comportamiento de la producción de café en Colombia usando técnicas de analítica de datos

El objeto de este estudio fue la identificación de tendencias en la producción de café. Donde se analizaron estadísticas cafeteras a nivel nacional, considerando reportes de la Organización Internacional del Café (ICO), el Sistema de Información Agropecuaria y la Federación Nacional de Cafeteros (FNC). Los factores relacionados con la variabilidad climática, el incremento del precio de producción, la escasez de mano de obra y la volatilidad del precio del café afectan la industria cafetera y pueden ser causas estructurales de las tendencias decrecientes en la producción, encontradas en algunos países productores y en algunos departamentos tradicionalmente caficultores de Colombia. La inteligencia de negocios, vela por la democratización de los datos, por tener una madurez de ellos y contar con una analítica predicativa.The object of this study was the identification of trends in coffee production. Where coffee statistics were analyzed at the national level, considering reports from the International Coffee Organization (ICO), the Agricultural Information System and the National Federation of Coffee Growers (FNC). Factors related to climate variability, increased production prices, labor shortages, and coffee price volatility affect the coffee industry and may be structural causes of the declining trends in production found in some producing countries. and in some traditionally coffee-growing departments of Colombia. Business intelligence ensures the democratization of data, for having a maturity of them and having a predictive analytics.PregradoIngeniero(a) de Sistemas y ComputaciónTABLA DE CONTENIDO INTRODUCCIÓN ................................................................................. 1 CAPITULO I. ....................................................................................... 2 1. GENERALIDADES .......................................................................... 2 1.1 PROBLEMA .................................................................................. 2 1.2 JUSTIFICACIÓN ........................................................................... 5 1.3 OBJETIVOS .................................................................................. 6 1.3.1 Objetivo General ......................................................................... 6 1.3.2 Objetivos Específicos ................................................................. 6 1.4 METODOLOGIA ............................................................................ 7 CAPITULO II. ...................................................................................... 9 2. ESTADO DEL ARTE ....................................................................... 9 2.1 MARCO CONCEPTUAL ................................................................ 9 2.2 MARCO TEORICO .......................................................................12 2.2.1 Apache Hadoop .........................................................................12 2.3 MARCO REFERENCIAL ..............................................................15 2.4 MARCO HISTORICO ...................................................................16 CAPITULO III .....................................................................................17 3. IDENTIFICACION DE FACTORES SOCIOECONOMICOS Y AMBIENTALES ..................................................................................17 3.1 RELACION ENTRE LAS EXPORTACIONES Y EL PRECIO EN LOS MERCADOS CONTRA LA PRODUCCION DEL CAFE ..............19 3.2 APORTE .......................................................................................21 3.3 CAPTACION DE LA INFORMACION ...........................................21 3.4 MANEJO DE LA INFORMACION .................................................23 3.5 VISUALIZACION ..........................................................................24 3.5 ANALISIS DE LA INFORMACION ................................................30 CAPITULO IV. ....................................................................................39 4. RESULTADOS ...............................................................................39 CAPITULO V. .....................................................................................41 5. CONCLUSIONES ...........................................................................41 CAPITULO VI. ....................................................................................43 6. BIBLIOGRAFIA ...............................................................................4

Factores socioeducativos asociados a la percepción de la investigación entre estudiantes de estomatología en una universidad cubana.

Introduction: Conducting research at the undergraduate level is essential to prepare future professionals for the process of properly channeling their scientific interest and provision of care. However, the factors influencing the degree of participation of students in research are unknown. Aim: To determine the socio-educational factors associated with the perception of research among stomatology students in a Cuban university. Materials and Methods: Cross-sectional analytical study conducted on 166 stomatology students. Participants received an anonymous, validated and self-administered questionnaire designed to collect information on the perception of scientific research within the educational spectrum, which included elements related to writing, participation, scientific publication and the interest of pursuing a career in research. Using associative statistics, those who had an overall positive perception of research were considered interested and were assessed according to socio-educational variables. Results: Students showed a positive perception of scientific research; however, when asked if they wanted to pursue a career in research, only 61.4% responded affirmatively. This positive perception increases with the progression of academic years (p<0.04), is higher in males (p=0.030) and in students who perform assistantships in core courses (p=0.001). Conclusions: There is a positive perception of scientific research, which tends to be intensified by academic progression and other factors. This must be considered by educational institutions in order to generate strategies as a response.Introducción: La investigación en el pregrado constituye una forma importante de preparar a los futuros profesionales para encauzar sus inquietudes científicas y la labor asistencial. Sin embargo, se desconocen los factores que influyen en la participación de los estudiantes en investigación. Objetivo: Determinar los factores socioeducativos asociados a la percepción de la investigación en estudiantes de estomatología de una universidad cubana. Materiales y Métodos: Estudio transversal-analítico, realizado en 166 estudiantes de estomatología. Los participantes recibieron un cuestionario anónimo, validado y autoadministrado para recoger información sobre la percepción de la investigación científica, que incluyó elementos relacionados con la redacción, la participación, la publicación científica y el interés de hacer una carrera de investigación en el futuro. A los que tuvieron una mejor percepción de la investigación se les consideró como interesados, y se les cruzó según sus variables socioeducativas, con estadísticos de asociación. Resultados: Los estudiantes mostraron una percepción positiva de la investigación científica; sin embargo, cuando se les preguntó si querían hacer una carrera de investigación en el futuro solo el 61,4% respondió afirmativamente. La percepción aumenta según progresan los años académicos (p<0,04), es mayor en los hombres (p=0,030) y en los estudiantes que realizan ayudantía en las cátedras de la carrera (p=0,001). Conclusiones: Existe una percepción positiva con respecto a la investigación científica, la cual aumentan conforme lo hacen los años académicos y otros factores; esto debe tomarse en cuenta para generar estrategias por parte de las instituciones educativas

ACTIVIDAD EXTRACTO ETANÓLICO DE HOJA DE Allium Sativum (Ajo) SOBRE Staphylococcus Aureus

Es conocida la actividad antimicrobiana del ajo como medida de tratamiento tradicional, el presente estudio tuvo como objetivo determinar la actividad del extracto etanólico de hoja de Allium sativum (ajo) sobre Staphylococcus aureus para lo cual se empleo un preparado preliminar utilizado para tratar infecciones fúngicas; empleando un extracto etanólico con características propias de la hoja de Allium cepa. Para valorar los efectos inhibitorios se utilizó la metodología tradicional que sustenta la halometría desarrollada. Logrando evidenciar una interacción farmacognosica. Se utilizó cepas de Staphylococcus aureus obtenidas de aislamiento de laboratorio clínico particular que fueron enfrentadas con concentraciones al 5%, 10%, 20% y 30% de una solución stock (extracto etanólico obtenido de las hojas), comprobándose un efecto en halos de inhibición de 10 nm, 15 nm y 18 nm son más efectivos, mientras que el 30% es más efectivo

Propuesta de Supply Chain Management y Logística para la empresa distribuidora & comercializadora Aguazul DCA

Tablas relacionando los resultados de las encuestas, enlaces de las encuestas realizadas, Formato de estudio para la selección de proveedores y relaciones comercialesEl Presente proyecto de grado tiene como objetivo el definir el enfoque del Supply Chain Management y Logística de la empresa Distribuidora & Comercializadora Aguazul DCA, por cinco estudiantes del Diplomado de Profundización Supply Chain Management y Logística integrantes del grupo 207115_18, el cual busca identificando los actores de la cadena de suministros desde los proveedores y clientes y las estructuras y procesos logísticos y cadena de Suministro de la empresa, mediante el uso de modelos propios de la disciplina; Configurando la red de cadena de suministro usando para ello el enfoque del GSCF. Para esto, se hace necesario describir como aplicarían los ocho procesos estratégicos según el Global Supply Chain Forum (GSCF). La distribución física es un propulsor estratégico para la economía de cualquier empresa por lo que de esta dependen la variación de los costos de la cadena de suministros que afectan de forma positiva o negativa en la rentabilidad de las empresas. Entre los temas a tratar en el presente documento se pueden observar el control de gestión juega un papel muy importante en la empresa, así como también tener actualizado las redes de logística y tener una buena planificación de la cadena de suministros. También se afianzarán conocimientos de SCM y logística de acuerdo al enfoque de APICS-SCOR, describiremos los procesos para la empresa Distribuidora & Comercializadora Aguazul DCA de acuerdo al enfoque anteriormente mencionado. De igual forma se comparará la posición de Colombia frente a otros seis países del mundo, en términos de logística según informe del Banco Mundial, junto con diagramas de flujo en que se representan los procesos de información, producto y dinero de la empresa Distribuidora & Comercializadora Aguazul DCA. La implementación de estrategias de DRP y TMS para la empresa Distribuidora & Comercializadora Aguazul DCA, verificando que los beneficios sean bien aprovechados en la gestión de los procesos logísticos y las estrategias que se podrían implementar para la distribución de la mercancía. Se crearan estrategias para la distribución en la organización Distribuidora & Comercializadora Aguazul DCA, con el fin de ser más competitiva en el mercado, al mismo tiempo que se busca generar un análisis de los diferentes métodos para gestionar la entrega de los productos manteniendo la calidad y generar la rentabilidad esperada por la organización, de esta forma mantener una comunicación directa con todos los actores que hacen parte de la cadena de distribución para mantener un control en la gestión del pedido, fomentando una rotación de los inventarios, esto logrando un mayor alcance para la compañía, también logra la planificación rigurosa en la entrega, mejorando la marca, su posicionamiento, y la imagen con el consumidor final quien tendrá una percepción positiva de la organización.The objective of this degree project is to define the approach of the Supply Chain Management and Logistics of the company Distribuidora & Comercializadora Aguazul DCA, by five students of the Diploma of Deepening Supply Chain Management and Logistics members of the group 207115_18, which seeks to identify the actors of the supply chain from suppliers and customers and the structures and logistics processes and supply chain of the company, through the use of models of the discipline; Configuring the supply chain network using the GSCF approach. For this, it is necessary to describe how the eight strategic processes would be applied according to the Global Supply Chain Forum (GSCF). Physical distribution is a strategic driver for the economy of any company, which is why the variation in the costs of the supply chain depends on it, which positively or negatively affects the profitability of companies. Among the topics to be discussed in this document, management control plays a very important role in the company, as well as keeping logistics networks up-to-date and having good supply chain planning. Knowledge of SCM and logistics will also be strengthened according to the APICS-SCOR approach, we will describe the processes for the company Distribuidora & Comercializadora Aguazul DCA according to the aforementioned approach. In the same way, the position of Colombia will be compared against six other countries in the world, in terms of logistics according to the World Bank report, together with flow charts that represent the information, product and money processes of the Distributor & Marketing company. Aguazul DCA. The implementation of DRP and TMS strategies for the company Distribuidora & Comercializadora Aguazul DCA, verifying that the benefits are well used in the management of logistics processes and the strategies that could be implemented for the distribution of merchandise. Strategies will be created for the distribution in the organization Distribuidora & Comercializadora Aguazul DCA, in order to be more competitive in the market, at the same time that it seeks to generate an analysis of the different methods to manage the delivery of the products maintaining the quality and generate the profitability expected by the organization, in this way maintain direct communication with all the actors that are part of the distribution chain to maintain control in the management of the order, promoting a rotation of inventories, this achieving a greater scope for The company also achieves rigorous delivery planning, improving the brand, its positioning, and the image with the final consumer who will have a positive perception of the organization

First wave of COVID-19 in Venezuela:Epidemiological, clinical, and paraclinical characteristics of first cases

The coronavirus disease 2019 (COVID-19) pandemic has particularly affected countries with weakened health services in Latin America, where proper patient management could be a critical step to address the epidemic. In this study, we aimed to characterize and identify which epidemiological, clinical, and paraclinical risk factors defined COVID-19 infection from the first confirmed cases through the first epidemic wave in Venezuela. A retrospective analysis of consecutive suspected cases of COVID-19 admitted to a sentinel hospital was carried out, including 576 patient cases subsequently confirmed for severe acute respiratory syndrome coronavirus 2 infection. Of these, 162 (28.1%) patients met the definition criteria for severe/critical disease, and 414 (71.2%) were classified as mild/moderate disease. The mean age was 47 (SD 16) years, the majority of which were men (59.5%), and the most frequent comorbidity was arterial hypertension (23.3%). The most common symptoms included fever (88.7%), headache (65.6%), and dry cough (63.9%). Severe/critical disease affected mostly older males with low schooling (p < 0.001). Similarly, higher levels of glycemia, urea, aminotransferases, total bilirubin, lactate dehydrogenase, and erythrocyte sedimentation rate were observed in severe/critical disease patients compared to those with mild/moderate disease. Overall mortality was 7.6% (44/576), with 41.7% (28/68) dying in hospital. We identified risk factors related to COVID-19 infection, which could help healthcare providers take appropriate measures and prevent severe clinical outcomes. Our results suggest that the mortality registered by this disease in Venezuela during the first epidemic wave was underestimated. An increase in fatalities is expected to occur in the coming months unless measures that are more effective are implemented to mitigate the epidemic while the vaccination process is ongoing

Global unmet needs in cardiac surgery

More than 6 billion people live outside industrialized countries and have insufficient access to cardiac surgery. Given the recently confirmed high prevailing mortality for rheumatic heart disease in many of these countries together with increasing numbers of patients needing interventions for lifestyle diseases due to an accelerating epidemiological transition, a significant need for cardiac surgery could be assumed. Yet, need estimates were largely based on extrapolated screening studies while true service levels remained unknown. A multi-author effort representing 16 high-, middle-, and low-income countries was undertaken to narrow the need assessment for cardiac surgery including rheumatic and lifestyle cardiac diseases as well as congenital heart disease on the basis of existing data deduction. Actual levels of cardiac surgery were determined in each of these countries on the basis of questionnaires, national databases, or annual reports of national societies. Need estimates range from 200 operations per million in low-income countries that are nonendemic for rheumatic heart disease to >1,000 operations per million in high-income countries representing the end of the epidemiological transition. Actually provided levels of cardiac surgery range from 0.5 per million in the assessed low- and lower-middle income countries (average 107 ± 113 per million; representing a population of 1.6 billion) to 500 in the upper-middle-income countries (average 270 ± 163 per million representing a population of 1.9 billion). By combining need estimates with the assessment of de facto provided levels of cardiac surgery, it emerged that a significant degree of underdelivery of often lifesaving open heart surgery does not only prevail in low-income countries but is also disturbingly high in middle-income countries

Sodium restriction in patients with chronic heart failure and reduced ejection fraction: A randomized controlled trial

Background: Sodium restriction is recommended for patients with heart failure (HF) despite the lack of solid clinical evidence from randomized controlled trials. Whether or not sodium restrictions provide beneficial cardiac effects is not known. Methods: The present study is a randomized, double-blind, controlled trial of stable HF patients with ejection fraction ≤ 40%. Patients were allocated to sodium restriction (2 g of sodium/day) vs. control (3 g of sodium/day). The primary outcome was change in N-terminal pro-B-type natriuretic peptide (NT-proBNP) at 20 weeks. Secondary outcomes included quality of life and adverse safety events (HF readmission, blood pressure or electrolyte abnormalities). Results: Seventy patients were enrolled. Median baseline sodium consumption was 3268 (2225–4537) mg/day. Adherence to the intervention based on 24-hour urinary sodium was 32%. NT-proBNP and quality of life did not significantly change between groups (p &gt; 0.05 for both). Adverse safety events were not significantly different between the arms (p &gt; 0.6 for all). In the per protocol analysis, patients who achieved a sodium intake &lt; 2500 mg/day at the  intervention conclusion showed improvements in NT-proBNP levels (between-group difference: –55%, 95% confidence interval –27 to –73%; p = 0.002) and quality of life (between-group difference –11 ± 5 points; p = 0.04). Blood pressure decreased in patients with lower sodium intake (between-group difference –9 ± 5 mmHg; p = 0.05) without significant differences in symptomatic hypotension or other safety events (p &gt; 0.3 for all). Conclusions: Adherence assessed by 24-hour natriuresis and by the nutritionist was poor. The group allocated to sodium restriction did not show improvement in NT-proBNP. However, patients who achieved a sodium intake &lt; 2500 mg/day appeared to have improvements in NT-proBNP and quality of life without any adverse safety signals. ClinicalTrials.gov Identifier: NCT03351283