Managing Climatic Risks to Combat Land Degradation and Enhance Food security: Key Information Needs

This paper discusses the key information needs to reduce the negative impacts of weather variability and climate change on land degradation and food security, and identifies the opportunities and barriers between the information and services needed. It suggests that vulnerability assessments based on a livelihood concept that includes climate information and key socio-economic variables can overcome the narrow focus of common one-dimensional vulnerability studies. Both current and future climatic risks can be managed better if there is appropriate policy and institutional support together with technological interventions to address the complexities of multiple risks that agriculture has to face. This would require effective partnerships among agencies dealing with meteorological and hydrological services, agricultural research, land degradation and food security issues. In addition a state-of-the-art infrastructure to measure, record, store and disseminate data on weather variables, and access to weather and seasonal climate forecasts at desired spatial and temporal scales would be needed

Optimization of a 3x3 focusing array for heavy ion drivers

A heavy ion driver for inertial fusion will accelerate an array of beams through common induction cores and then direct the beams onto the DT target. An array of quadrupole focusing magnets is used to prevent beam expansion from space charge forces. In the array, the magnet fields from the coils embracing the beams are coupled, which reduces the cost of superconductor and increases the focusing power. The challenges in designing such an array are meeting the strict requirements for the quadrupole field inside the beam pipes and preventing stray fields outside. We report our optimization effort on designing such an array and show that 3 x 3 or larger arrays are feasible and practical to build with flat racetrack coils

Sextupole correction magnets for the Large Hadron Collider

About 2500 superconducting sextupole corrector magnets (MCS) are needed for the Large Hadron Collider (LHC) at CERN to compensate persistent current sextupole fields of the main dipoles. The MCS is a cold bore magnet with iron yoke. The coils are made from a NbTi conductor, which is cooled to 1.9 K. In the original CERN design 6 individual sub-coils, made from a monolithic composite conductor, are assembled and spliced together to form the sextupole. The coils are individually wound around precision-machined central islands and stabilized with matching saddle pieces at both ends. The Advanced Magnet Lab, Inc. (AML) has produced an alternative design, which gives improved performance and reliability at reduced manufacturing cost. In the AML design, the magnet consists of three splice-free sub-coils, which are placed with an automated winding process into pockets of prefabricated G-11 support cylinders. Any assembly process of sub-coils with potential misalignment is eliminated. The AML magnet uses a Kapton-wrapped mini-cable, which allows helium penetration into the vicinity of the conductor, increasing its cryogenic stability. Eliminating all internal splices from the magnet significantly reduces heat loads and the risk of magnet failure during operation. A tested prototype reached the critical current limit of the conductor in the first quench. (3 refs)

Foresight and trade-off analyses : tools for science strategy development in agriculture and food systems research

Foresight and trade-off analyses offer organizations such as CGIAR an opportunity to better prepare for alternative futures through adaptive research strategy and management. This essay introduces a set of papers that explore foresight and trade-off analyses within the context of the major reforms now occurring in the CGIAR. We tease out lessons not only for One CGIAR, but also for international development research organizations more broadly.Publisher PDFPeer reviewe

Follicular Delivery of Caffeine from a Shampoo for Hair Retention

A key factor in the prevention of hair loss is the provision of optimal conditions on the scalp. In this regard, reduction of oxidative stress on the scalp is one critical requirement to support the hair follicles to function optimally. Recently, a novel shampoo formulation technology containing anti-oxidants such as piroctone olamine has been demonstrated to improve hair retention based on micellar degradation and coacervation effects. Caffeine has also been shown to exhibit anti-oxidant activity including the ability to inhibit lipid peroxidation. As with piroctone olamine, it is expected that follicular delivery of caffeine will enhance its anti-oxidant activity in a region that will be beneficial for hair retention. In this study, two shampoo formulations as well as a control formulation were applied to the calf area of n = 9 male participants. The technique of differential tape stripping was applied to obtain the caffeine penetrated to the stratum corneum and to the hair follicles. Isotope-dilution liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was performed to demonstrate caffeine follicular delivery from the shampoo formulas. The results showed that the percentage of caffeine recovered in the hair follicles was 8–9% of the caffeine absorbed into the skin and matched an existing caffeine-based shampoo. In conclusion, a novel shampoo formulation technology has been developed that effectively delivers beneficial anti-oxidants to improve hair retention. This new shampoo is expected to be especially useful in the goal of retaining hair during aging

Spatio-Temporal Model-Checking of Cyber-Physical Systems Using Graph Queries

We explore the application of graph database technology to spatio-temporal model checking of cooperating cyber-physical systems-of-systems such as vehicle platoons. We present a translation of spatio-temporal automata (STA) and the spatio-temporal logic STAL to semantically equivalent property graphs and graph queries respectively. We prove a sound reduction of the spatio-temporal verification problem to graph database query solving. The practicability and efficiency of this approach is evaluated by introducing NeoMC, a prototype implementation of our explicit model checking approach based on Neo4j. To evaluate NeoMC we consider case studies of verifying vehicle platooning models. Our evaluation demonstrates the effectiveness of our approach in terms of execution time and counterexample detection

Nanocrystals for Improved Drug Delivery of Dexamethasone in Skin Investigated by EPR Spectroscopy

Nanocrystals represent an improvement over the traditional nanocarriers for dermal application, providing the advantages of 100% drug loading, a large surface area, increased adhesion, and the potential for hair follicle targeting. To investigate their advantage for drug delivery, compared to a base cream formulation, dexamethasone (Dx), a synthetic glucocorticoid frequently used for the treatment of inflammatory skin diseases, was covalently linked with the paramagnetic probe 3-(carboxy)-2,2,5,5-tetramethyl-1-pyrrolidinyloxy (PCA) to DxPCA. To investigate the penetration efficiency between these two vehicles, electron paramagnetic resonance (EPR) spectroscopy was used, which allows the quantification of a spin-labeled drug in different skin layers and the monitoring of the drug release. The penetration behavior in excised healthy and barrier-disrupted porcine skin was monitored by EPR, and subsequently analyzed using a numerical diffusion model. As a result, diffusion constants and free energy values in the different layers of the skin were identified for both formulations. Dx-nanocrystals showed a significantly increased drug amount that penetrated into viable epidermis and dermis of intact (factor 3) and barrier-disrupted skin (factor 2.1) compared to the base cream formulation. Furthermore, the observed fast delivery of the spin-labeled drug into the skin (80% DxPCA within 30 min) and a successive release from the aggregate unit into the viable tissue makes these nanocrystals very attractive for clinical applications

A multistage sequencing strategy pinpoints novel candidate alleles for Emery-Dreifuss muscular dystrophy and supports gene misregulation as its pathomechanism

BACKGROUND: As genome-wide approaches prove difficult with genetically heterogeneous orphan diseases, we developed a new approach to identify candidate genes. We applied this to Emery-Dreifuss muscular dystrophy (EDMD), characterised by early onset contractures, slowly progressive muscular wasting, and life-threatening heart conduction disturbances with wide intra- and inter-familial clinical variability. Roughly half of EDMD patients are linked to six genes encoding nuclear envelope proteins, but the disease mechanism remains unclear because the affected proteins function in both cell mechanics and genome regulation. METHODS: A primer library was generated to test for mutations in 301 genes from four categories: (I) all known EDMD-linked genes; (II) genes mutated in related muscular dystrophies; (III) candidates generated by exome sequencing in five families; (IV) functional candidates - other muscle nuclear envelope proteins functioning in mechanical/genome processes affected in EDMD. This was used to sequence 56 unlinked patients with EDMD-like phenotype. FINDINGS: Twenty-one patients could be clearly assigned: 18 with mutations in genes of similar muscular dystrophies; 3 with previously missed mutations in EDMD-linked genes. The other categories yielded novel candidate genes, most encoding nuclear envelope proteins with functions in gene regulation. INTERPRETATION: Our multi-pronged approach identified new disease alleles and many new candidate EDMD genes. Their known functions strongly argue the EDMD pathomechanism is from altered gene regulation and mechanotransduction due to connectivity of candidates from the nuclear envelope to the plasma membrane. This approach highlights the value of testing for related diseases using primer libraries and may be applied for other genetically heterogeneous orphan diseases. FUNDING: The Wellcome Trust, Muscular Dystrophy UK, Medical Research Council, European Community's Seventh Framework Programme "Integrated European -omics research project for diagnosis and therapy in rare neuromuscular and neurodegenerative diseases (NEUROMICS)"

Evolution of the One CGIAR’s research and innovation portfolio to 2030 : approaches, tools, and insights after the reform

In this perspective, we offer insights into the evolution of CGIAR’s research and innovation portfolio from 2019 to 2023, underpinning the transformative journey towards One CGIAR. With this contribution, we aim to strengthen the social and environmental sustainability components of allied, future Research for Development (R4D) portfolios. We explore three interlinked operational frameworks—Quality of Research for Development (QoR4D), Comparative Advantage (CA) Analysis, and Inclusive Innovation—and present practical tools and lessons for enhancing the quality and impact of R4D initiatives. This work is based on insights gained by the Independent Science for Development Council (ISDC) during the review of proposals for the current One CGIAR research portfolio. QoR4D’s four dimensions (relevance, scientific credibility, legitimacy, and effectiveness) guide research strategy, portfolio development, evaluation and performance standards, fostering intentional design and transparent assessment. CA Analysis leverages organisational strengths, facilitating purposeful partnerships, and strategic resource allocation. Inclusive Innovation emphasises stakeholder inclusivity, amplifying legitimacy, relevance, and effectiveness. Insights are drawn from the application of these frameworks, highlighting the importance of collaboration, the need for a mindset shift and institutional reform, specialisation, and impact maximisation. By adopting these lessons, CGIAR and allied organisations can collectively address global food system challenges more effectively, driving sustainable agricultural innovation and societal transformation. This article aims to contribute to advancing sustainable agriculture and underscores the significance of systemic collaborations in creating more resilient and equitable food systems.Publisher PDFPeer reviewe

MidA is a putative methyltransferase that is required for mitochondrial complex I function

10 páginas, 6 figuras.-- et al.Dictyostelium and human MidA are homologous proteins that belong to a family of proteins of unknown function called DUF185. Using yeast two-hybrid screening and pull-down experiments, we showed that both proteins interact with the mitochondrial complex I subunit NDUFS2. Consistent with this, Dictyostelium cells lacking MidA showed a specific defect in complex I activity, and knockdown of human MidA in HEK293T cells resulted in reduced levels of assembled complex I. These results indicate a role for MidA in complex I assembly or stability. A structural bioinformatics analysis suggested the presence of a methyltransferase domain; this was further supported by site-directed mutagenesis of specific residues from the putative catalytic site. Interestingly, this complex I deficiency in a Dictyostelium midA- mutant causes a complex phenotypic outcome, which includes phototaxis and thermotaxis defects. We found that these aspects of the phenotype are mediated by a chronic activation of AMPK, revealing a possible role of AMPK signaling in complex I cytopathology.This work was supported by grants BMC2006-00394 and BMC2009-09050 to R.E. from the Spanish Ministerio de Ciencia e Innovación; to P.R.F. from the Thyne Reid Memorial Trusts and the Australian Research Council; to A.V. and O.G. from the Spanish National Bioinformatics Institute (www.inab.org), a platform of Genome Spain; to R.G. from the Fondo de Investigaciones Sanitarias, Instituto de Salud Carlos III, Spain (PI070167) and from the Comunidad de Madrid (GEN-0269/2006). S.C. is supported by a research contract from Consejería de Educación de la Comunidad de Madrid y del Fondo Social Europeo (FSE).Peer Reviewe