Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey

A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae . Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [a-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. The regenerated shoots transferred to rooting medium (MS with 0.5 mg/l indole-3-butyric acid) were successfully rooted (100%) and showed rapid elongation. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite

Responses of Some Dwarf Bean (Phaseolus vulgaris L.) Genotypes Grown in Turkey to Zinc and Boron Applications

This study was conducted to determine the effects of boron (B) and zinc (Zn) applications on biomass, dry weight content and grain yield of 25 registered and local populations of dwarf bean genotypes tested. Plants were grown for 3 B and 3 Zn doses (0, 5, 10 mg kg-1 each). The trial was conducted in three replicates within a controlled glasshouse according to randomized plots factorial design. Results have shown that B and Zn applications considerably effect on the dwarf dry bean varieties in terms of biomass, dry weight and grain yield. From the genotypes used, Eskisehir-855, Karacasehir-90, Noyanbey, Terzibaba, Yakutiye, Fasulye sira, Yalova-17, Romano, Nazende, Seminis Gina, Yunus-90, Akman-98, Z¨ulbiye, Horoz fasulye and Kanada were found to be resistant to B deficiency while Sehirali-90, G¨oyn¨uk-98, Akdag, Sahin-90, ¨Onceler-98, Aras-98, Sarikiz, Magnum, May Gina and Efsane were the sensitive ones. The highest grain yield at 10 mg B kg-1 application was attained from the species Aras-98 and Kanada, hence they were classified as resistant to B toxicity. Zinc x genotype interaction was found significant from the stand point of biomass, dry weight and grain yield. Akman-98, Karacasehir-90, Noyanbey, Terzibaba, Sahin-90, Horoz fasulye, Nazende and Efsane were tolerant to Zn deficiency, while G¨oyn¨uk-98, Aras-98 and Fasulye sira were moderately tolerant. On the other hand, the varieties Eskisehir-855, Yunus-90, Sehirali-90, ¨Onceler-98, Z¨ulbiye, Yakutiye, Romano and Kanada were moderately sensitive to Zn deficiency, while the species of Akdag, Sarikiz, Magnum, Seminis Gina, May Gina and Yalova-17 were highly sensitive

Responses of Some Dwarf Bean (Phaseolus vulgaris L.) Genotypes Grown in Turkey to Zinc and Boron Applications

This study was conducted to determine the effects of boron (B) and zinc (Zn) applications on biomass, dry weight content and grain yield of 25 registered and local populations of dwarf bean genotypes tested. Plants were grown for 3 B and 3 Zn doses (0, 5, 10 mg kg-1 each). The trial was conducted in three replicates within a controlled glasshouse according to randomized plots factorial design. Results have shown that B and Zn applications considerably effect on the dwarf dry bean varieties in terms of biomass, dry weight and grain yield. From the genotypes used, Eskisehir-855, Karacasehir-90, Noyanbey, Terzibaba, Yakutiye, Fasulye sira, Yalova-17, Romano, Nazende, Seminis Gina, Yunus-90, Akman-98, Z¨ulbiye, Horoz fasulye and Kanada were found to be resistant to B deficiency while Sehirali-90, G¨oyn¨uk-98, Akdag, Sahin-90, ¨Onceler-98, Aras-98, Sarikiz, Magnum, May Gina and Efsane were the sensitive ones. The highest grain yield at 10 mg B kg-1 application was attained from the species Aras-98 and Kanada, hence they were classified as resistant to B toxicity. Zinc x genotype interaction was found significant from the stand point of biomass, dry weight and grain yield. Akman-98, Karacasehir-90, Noyanbey, Terzibaba, Sahin-90, Horoz fasulye, Nazende and Efsane were tolerant to Zn deficiency, while G¨oyn¨uk-98, Aras-98 and Fasulye sira were moderately tolerant. On the other hand, the varieties Eskisehir-855, Yunus-90, Sehirali-90, ¨Onceler-98, Z¨ulbiye, Yakutiye, Romano and Kanada were moderately sensitive to Zn deficiency, while the species of Akdag, Sarikiz, Magnum, Seminis Gina, May Gina and Yalova-17 were highly sensitive

E-Government Education in Turkish Public Administration Graduate Programs: Past, Present, and Future

WOS: 000407817400012The study of e-government is becoming increasingly important around the world in the field of public administration. This article examines the historical development, current status, and future prospects of graduate e-government courses in Turkish public administration programs. To that end, we performed content analysis of e-government course syllabi and evaluated relevant archival documents. We then conducted semistructured surveys of past and current instructors of graduate e-government courses. The article concludes by discussing the future prospects of e-government education in Turkey, including the problems that instructors need to solve in order to improve instruction and the relevance and impact of such courses on students and faculty of public administration programs in Turkey and elsewhere

Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey

A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae . Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [a-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. The regenerated shoots transferred to rooting medium (MS with 0.5 mg/l indole-3-butyric acid) were successfully rooted (100%) and showed rapid elongation. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite

Modified naples prognostic score as a potential predictor for coronary slow flow: a retrospective observational study

The Naples prognostic score (NPS) predicts patient survival in gastroesophageal cancer using parameters related to nutritional and inflammatory status. These parameters include risk factors for coronary endothelial dysfunction except for low total cholesterol. Therefore, we modified the score (mNPS) to include high cholesterol, a risk factor for coronary endothelial dysfunction. We aimed to evaluate the relationship between mNPS and the angiographic epicardial coronary slow flow phenomenon (CSFP). This retrospective study included 301 patients with coronary slow flow who underwent coronary angiography between 2018 and 2022. The mNPS parameters were calculated and the population was divided into three groups based on the calculated parameters. Angiographic findings were classified in the left anterior descending (LAD), circumflex (Cx), right coronary (RCA) arteries, and three coronary arteries together. Statistical analyses were performed to identify mNPS as predictors of a slow flow phenomenon. Participants were divided into mNPS Group 1 (n=63), mNPS Group 2 (n=201), and mNPS Group 3 (n=37). No significant differences were observed in age, gender, or medications among the mNPS groups. The RCA had a statistically significant association with mNPS groups for slow flow phenomenon (p=0.006). Considering all three coronary arteries, the association with mNPS groups was also significant (p=0.005). White blood cell and lymphocyte counts showed significant differences. Compared with group 1, group 3 had 4.11 times more coronary artery slow flow. Our study suggests that the mNPS, integrating nutritional and inflammatory parameters along with high cholesterol, holds promise as a potential predictor for the coronary slow flow phenomenon. This could impact risk stratification and clinical management in this patient group. [Med-Science 2023; 12(4.000): 1227-31

Platelet/lymphocyte ratio and mean platelet volume in patients with granulomatosis with polyangiitis

Background Granulomatosis with polyangiitis (GPA) is a granulomatous necrotizing vasculitis with high morbidity and mortality. Anti-neutrophil cytoplasmic antibody is a valuable diagnostic marker, however its titer lacks predictive value for the severity of organ involvement. Platelet to lymphocyte ratio (PLR) and mean platelet volume (MPV) has been regarded as a potential marker in assessing systemic inflammation. We aimed to explore the value of PLR and MPV in the assessment of disease activity and manifestations of disease in GPA. Methods 56 newly diagnosed GPA patients and 53 age-sex matched healthy controls were included in this retrospective and cross-sectional study with comparative group. Complete blood count was performed with Backman Coulter automatic analyzer, erythrocyte sedimentation rate (ESR) with Westergen method and C-reactive protein (CRP) levels with nephelometry. The PLR was calculated as the ratio of platelet and lymphocyte counts. Result Compared to control group, ESR, CRP and PLR were significantly higher and MPV significantly lower in GPA patients. In patients group, PLR was positively correlated with ESR and CRP (r = 0.39, p = 0.005 and r = 0.51, p < 0.001, respectively). MPV was negatively correlated with ESR and CRP (r = - 0.31, p = 0.028 and r = - 0.34 p = 0.014, respectively). Patients with renal involvement had significantly higher PLR than patients without renal involvement (median:265.98, IQR:208.79 vs median:180.34 IQR:129.37, p = 0.02). PLR was negatively correlated with glomerular filtration rate (r = - 0.27, p = 0.009). A cut-off level of 204 for PLR had 65.6\% sensitivity and 62.5 specificity to predict renal involvement. Conclusion PLR exhibit favorable diagnostic performance in predicting renal involvement in patients with GPA