Continuous measurements of real-life bidirectional pedestrian flows on a wide walkway

Employing partially overlapping overhead \kinectTMS sensors and automatic pedestrian tracking algorithms we recorded the crowd traffic in a rectilinear section of the main walkway of Eindhoven train station on a 24/7 basis. Beside giving access to the train platforms (it passes underneath the railways), the walkway plays an important connection role in the city. Several crowding scenarios occur during the day, including high- and low-density dynamics in uni- and bi-directional regimes. In this paper we discuss our recording technique and we illustrate preliminary data analyses. Via fundamental diagrams-like representations we report pedestrian velocities and fluxes vs. pedestrian density. Considering the density range $0$ - $1.1\,$ped/m$^2$, we find that at densities lower than $0.8\,$ped/m$^2$ pedestrians in unidirectional flows walk faster than in bidirectional regimes. On the opposite, velocities and fluxes for even bidirectional flows are higher above $0.8\,$ped/m$^2$

Heart rate responses and technical-tactical aspects of official 5-a-side youth soccer matches played on soil and artificial turf.

Using a randomized crossover design, this study aimed to compare the heart rate (HR) responses and match analysis parameters (i.e., type of action; the number of players involved in an action; the number of passes performed in a collective action; precision of the shots; lost balls; ball interceptions; dribblings; and tackles) of official 5-a-side youth male soccer matches played over 2 pitch surface (i.e., clay vs. artificial turf) conditions. Twenty-two young male soccer players (age 8.3 6 0.4 years) engaged in 2 consecutive 15-minute periods in each experimental condition. During the match, 53% of HR responses exceeded 85% of the individual HRpeak. No difference emerged between both pitch surfaces and match periods for HR and match analysis indicators. The two 15-minute periods resulted in being appropriate for 8-year-old players, in resembling the intensity of play and the intermittent nature of adult soccer. The similar HR responses and technical-tactical patterns observed on the 2 surfaces indicate that youth match play is not affected by differences in pitch surface. To support the development of individual and team skills of young soccer players, coaches are advised to prioritize game knowledge based on divergent thinking and experience, rather than traditional teaching based on instructions

The N-terminal intrinsically disordered domain of mgm101p is localized to the mitochondrial nucleoid.

The mitochondrial genome maintenance gene, MGM101, is essential for yeasts that depend on mitochondrial DNA replication. Previously, in Saccharomyces cerevisiae, it has been found that the carboxy-terminal two-thirds of Mgm101p has a functional core. Furthermore, there is a high level of amino acid sequence conservation in this region from widely diverse species. By contrast, the amino-terminal region, that is also essential for function, does not have recognizable conservation. Using a bioinformatic approach we find that the functional core from yeast and a corresponding region of Mgm101p from the coral Acropora millepora have an ordered structure, while the N-terminal domains of sequences from yeast and coral are predicted to be disordered. To examine whether ordered and disordered domains of Mgm101p have specific or general functions we made chimeric proteins from yeast and coral by swapping the two regions. We find, by an in vivo assay in S.cerevisiae, that the ordered domain of A.millepora can functionally replace the yeast core region but the disordered domain of the coral protein cannot substitute for its yeast counterpart. Mgm101p is found in the mitochondrial nucleoid along with enzymes and proteins involved in mtDNA replication. By attaching green fluorescent protein to the N-terminal disordered domain of yeast Mgm101p we find that GFP is still directed to the mitochondrial nucleoid where full-length Mgm101p-GFP is targeted

Recovery and performance in sport: Consensus statement

© 2018 Human Kinetics, Inc. The relationship between recovery and fatigue and its impact on performance has attracted the interest of sport science for many years. An adequate balance between stress (training and competition load, other life demands) and recovery is essential for athletes to achieve continuous high-level performance. Research has focused on the examination of physiological and psychological recovery strategies to compensate external and internal training and competition loads. A systematic monitoring of recovery and the subsequent implementation of recovery routines aims at maximizing performance and preventing negative developments such as underrecovery, nonfunctional overreaching, the overtraining syndrome, injuries, or illnesses. Due to the inter- and intraindividual variability of responses to training, competition, and recovery strategies, a diverse set of expertise is required to address the multifaceted phenomena of recovery, performance, and their interactions to transfer knowledge from sport science to sport practice. For this purpose, a symposium on Recovery and Performance was organized at the Technical University Munich Science and Study Center Raitenhaslach (Germany) in September 2016. Various international experts from many disciplines and research areas gathered to discuss and share their knowledge of recovery for performance enhancement in a variety of settings. The results of this meeting are outlined in this consensus statement that provides central definitions, theoretical frameworks, and practical implications as a synopsis of the current knowledge of recovery and performance. While our understanding of the complex relationship between recovery and performance has significantly increased through research, some important issues for future investigations are also elaborated

Natuur en biodiversiteit in de biologische markt. Verkenning van de mogelijkheden om natuur en biodiversiteit in de biologische markt te verwaarden

In deze studie zijn de mogelijkheden verkend om natuur en biodiversiteit op biologische melkveebedrijven te vermarkten. Er is consumentenonderzoek uitgevoerd, er zijn pakketten van maatregelen opgesteld en getoetst op hun bijdrage aan biodiversiteit en de haalbaarheid voor de biologische melkveehouders. De burger verwacht van boeren en tuinders dat ze niet alleen voedsel produceren, maar ook bijdragen aan natuur en biodiversiteit en aan een aantrekkelijk landschap. Daarbij groeit het besef dat dit alleen mogelijk is als boerenondernemers hiervoor een reële vergoeding ontvangen. Het project waarvan we hier verslag doen, heeft tot doel de mogelijkheden voor het vermarkten van bovenwettelijke prestaties op het gebied van natuur en biodiversiteit te verkennen en uit te werken in een certificeringsprogramma

Effizienz und Wettbewerb im deutschen Bankensektor

In diesem Papier geben wir einen Überblick über den Wettbewerb und die Effizienz im deutschen Bankensektor. Wir nehmen dabei die Entwicklung in den letzten 15 Jahren in den Blick. Frühere Studien für Deutschland weisen darauf hin, dass das deutsche Bankensystem durch monopolistische Konkurrenz und durch moderat hohe Kosteneffizienz gekennzeichnet ist. Zudem scheinen Wettbewerb und Effizienz positiv miteinander verbunden. Des weiteren bestanden deutliche Unterschiede zwischen großen und kleinen Banken. Kleine Banken bevorzugten mehr das traditionelle Bankgeschäft, geringere Risiken und haben eher auf regional segmentierten Märk ten agiert. Die gegenwärtige Krise könnte zu tieferen Änderungen im Bankensystem führen, mit möglichen Folgen für Wettbewerb und Effizienz.In this paper we review the main findings on competition and efficiency for the German banking sector. Our aim is to give a bird view of the evolution during the past 15 years. Previous studies on Germany point to a banking system dominated by monopolistic competition and moderate cost efficiency. Competition and efficiency seem to be positively related. Furthermore significant differences existed between small and large banks. The business model of small banks was often more traditional, they preferred less risk and they acted on more segmented regional markets. The current crisis may lead to deep changes of the banking system, potentially with consequences for competition and efficiency

Fat1 deletion promotes hybrid EMT state, tumour stemness and metastasis

FAT1, which encodes a protocadherin, is one of the most frequently mutated genes in human cancers1–5. However, the role and the molecular mechanisms by which FAT1 mutations control tumour initiation and progression are poorly understood. Here, using mouse models of skin squamous cell carcinoma and lung tumours, we found that deletion of Fat1 accelerates tumour initiation and malignant progression and promotes a hybrid epithelial-to-mesenchymal transition (EMT) phenotype. We also found this hybrid EMT state in FAT1-mutated human squamous cell carcinomas. Skin squamous cell carcinomas in which Fat1 was deleted presented increased tumour stemness and spontaneous metastasis. We performed transcriptional and chromatin profiling combined with proteomic analyses and mechanistic studies, which revealed that loss of function of FAT1 activates a CAMK2–CD44–SRC axis that promotes YAP1 nuclear translocation and ZEB1 expression that stimulates the mesenchymal state. This loss of function also inactivates EZH2, promoting SOX2 expression, which sustains the epithelial state. Our comprehensive analysis identified drug resistance and vulnerabilities in FAT1-deficient tumours, which have important implications for cancer therapy. Our studies reveal that, in mouse and human squamous cell carcinoma, loss of function of FAT1 promotes tumour initiation, progression, invasiveness, stemness and metastasis through the induction of a hybrid EMT state