Screening of Synergic Interactions of Anti-Angiogenic and Anti-Tumor Compounds

Solid cancers have several common characteristics that Hanahan & Weinberg named as the hallmarks of cancer. Angiogenesis is an essential hallmark of cancer because tumor cells need oxygen and nutrients delivered by the vascular system. In fact, tumor growth and metastasis are angiogenesis dependent, and microvascular endothelial cells recruited by tumors have become an important target in cancer therapy. Combinations of drugs with different modes of action may lead to enhanced antitumor and antiangiogenic effects without injuring the host. The combined use of two drugs may sometimes produce enhanced, unchanged or diminished effects in comparison with their individual effects. These three different types of behaviour of the interacting drugs are called synergy, additive/indifferent and antagonistic effects. In the present work, we analyze 105 paired combinations of 15 compounds, some described by our research group as potent antiangiogenic compounds, and others currently used in clinical therapy. Our results show synergistic effects of several paired combinations using the MTT assay. [Our experimental work is supported by grants BIO2014-56092-R (MINECO and FEDER), P12-CTS-1507 (Andalusian Government and FEDER) and funds from group BIO-267 (Andalusian Government). The "CIBER de Enfermedades Raras" is an initiative from the ISCIII (Spain)].This communication has the support of a travel grant "Universidad de Málaga.Campus de Excelencia Internacional Andalucía Tech".Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Mezclas asfálticas dosificadas con RCD para carreteras de bajo tráfico

[Resumen:] En este artículo se estudia la viabilidad técnica de utilizar áridos procedentes de residuos de construcción y demolición en mezclas asfálticas en caliente. Se eligió una mezcla gruesa con diferentes porcentajes de áridos reciclados. Los parámetros Marshall cumplen lo estipulado en el PG-3 para tráficos T3. Las mezclas asfálticas en caliente fabricadas con diferentes porcentajes de áridos reciclados tienen una mala adhesividad. Con áridos calizos se obtiene una mejor adhesividad. La adhesividad mejora notablemente al eliminar el mortero de cemento adherido a los áridos reciclados

Ensayos de resistencia de las mezclas asfálticas al ataque de carburantes. Recomendaciones sobre el tiempo de inmersión en baño

Este congreso tivo lugar na Coruña do 2 ao 4 de xullo de 2008.[Resumen:] Una de las limitaciones que tienen las mezclas asfálticas como material para pavimentación es su falta de resistencia al ataque de los carburantes derivados del petróleo. Existen diversos procedimientos para determinar la resistencia de los materiales asfálticos a la acción de los carburantes. En el caso de selladores y tratamientos superficiales pueden encontrarse diversas normas de ensayo, tanto en la series europeas como americanas. Sin embargo, en lo referido a la evaluación de mezclas asfálticas propiamente dichas, existe una notable falta de normalización. Esta ponencia estudia en laboratorio algunos aspectos fundamentales de los procedimientos para evaluar la resistencia de las mezclas asfálticas respecto a la acción de los carburantes. Como resultado de la investigación se hacen algunas recomendaciones de buena práctica para los procedimientos de pérdida de peso tras inmersión en carburante, con o sin cepillado posterior, y respecto a la estabilidad Marshall conservada tras inmersión. En particular, respecto al período de inmersión, uno de los factores con más influencia, a la vista de los resultados de laboratorio y su tratamiento estadístico, se recomienda que sea de 24 horas, excepto en el ensayo de pérdida de peso tras inmersión sin cepillado posterior, en el que los datos obtenidos resultan más significativos si el período de inmersión es de 7 días

Hot mix asphalt using C&D waste as coarse aggregates

[Abstract] This article evaluates the possibility of designing hot asphalt mix road pavements using Construction and demolition waste as coarse recycled aggregates. The percentages of recycled aggregates used in the mixtures were: 0%, 20%, 40% and 60%. Cement and lime were used as fillers. The mixtures made with coarse recycled aggregates complied with the Marshall technical specifications for low volume roads. The mixtures also showed good resistance to permanent deformation evaluated by means of wheel tracking tests. Nevertheless, the mixtures made with RA may have insufficient durability due to their high susceptibility to water action which was evaluated using stripping tests

Impact of construction and demolition waste aggregates on stripping in hot mix asphalt

[Abstract:] This paper analyzes the effect of water on the durability of hot asphalt mixtures made with recycled aggregates from construction and demolition debris. Indirect Tensile Stress tests were carried out to evaluate stripping behavior. The mixtures tested were fabricated with 0%, 20%, 40% and 60% recycled aggregates. Two types of natural aggregates were used: schist and calcite dolomite. An increase in the percentage of recycled aggregates was found to produce a decrease in the Tensile Stress Ratio of the hot asphalt mixtures. To study this phenomenon, two and three factor Analysis of Variance (ANOVA) were performed with Indirect Tensile Stress being used as the dependent variable. The factors studied were the percentage of recycled aggregates (0%, 20%, 40% and 60%), the moisture state (dry, wet) and the type of natural aggregate (schist, calcite). On the basis of the ANOVA results, it was found that the most important factor affecting resistance was the moisture state (dry, wet) of the specimens. The percentage of recycled aggregate also affected Indirect Tensile Stress, especially in the dry state. The type of natural aggregate did not have a significant effect on Indirect Tensile Stress. The hot asphalt mixture specimens made with different percentages of recycled aggregates from construction and demolition debris and of natural quarry aggregates showed poor stripping behaviour. This stripping behaviour can be related to both the poor adhesion of the recycled aggregates and the high absorption of the mortar of cement adhered to them.Ministerio de Educación y Ciencia; BIA 2006-14742-C02-01Xunta de Galicia; 07TMT012118P

Modelización numérica en 3D de materiales reciclados en frío in situ con emulsión bituminosa en carreteras de bajo tráfico de la República Sudafricana

Esta XII Jornada tuvo lugar en Madrid, el 30 y 31 de mayo de 2017.[Resumen:] En esta comunicación se hace una modelización numérica de secciones de firme recicladas “in situ” con emulsión asfáltica. Se analizan firmes constituidos por un 25 % de material bituminoso fresado (Reclaimed Asphalt Pavement, RAP) y un 75 % de áridos naturales (capa granular), tanto con un 1 % de cemento como sin él. Se modeliza el comportamiento resiliente y permanente de estos materiales. Se calculan las respuestas del firme bajo una carga de ruedas gemelas, con cuatro tipos de explanadas de diversas características. Se obtiene la ley de variación del módulo resiliente para las cuatro explanadas y para los dos materiales reciclados (con cemento y sin cemento). Se analiza finalmente la deformación permanente de estos materiales reciclados

NeuN/Fox-3 is an intrinsic component of the neuronal nuclear matrix

AbstractNeuN is an antigen detected in the nucleus of neurons in a wide range of vertebrates and so it is widely used as a tool for detecting neuronal cells. NeuN has been recently identified as Fox-3, a new member of the Fox-1 gene family of splicing factors. The predominant localization of NeuN/Fox-3 to neuronal nuclei and its role in splicing pose the question of the nuclear compartmentalization of such a protein. Here we provide evidence that NeuN/Fox-3 is an intrinsic component of the neuronal nuclear matrix and a reliable marker of nuclear speckles in neurons.Structured summaryMINT-7890176: Fox-3 (uniprotkb:B7ZC13) and Splicing factor SC35 (uniprotkb:Q6PDU1) colocalize (MI:0403) by fluorescence microscopy (MI:0416

Numerical Simulation of the Stiffness Evolution With Curing of Pavement Sections Rehabilitated Using Cold In-Place Recycling Technology

Financiado para publicación en acceso aberto: Universidade da Coruña/CISUG[Abstract] Cold in-place recycling (CIR) technologies are becoming one of the main bets in the road sector to promote the reduction of greenhouse gas emissions. This technique also contributes to the circular economy, reusing 100% of the RAP from worn roads. In this research, numerical simulations of CIR sections are presented. The nonlinear behaviour of the CIR-base material is modelled using three predictive models based on triaxial test results. Variations in the performance depending on the type of subbase, the curing of the CIR-base material and its thickness, and the effect of the wearing-course were analysed. The response of the sections with unbound granular subbase proved to be very sensitive to variations in the parameters studied, and the increase in CIR base thickness was beneficial, while the opposite occurred with a cement-treated subbase.The authors would like to acknowledge funding for the project BIA2016-80317-R from the Spanish Ministry of Economy and Competitiveness (MINECO), with an associated pre-doctoral scholarship for the training of research workers (FPI) BES-2017-079633. An special acknowledgement to the Universidade da Coruña/ CISUG for funding Elsevier’s open access charg