Editorial: Design and Implementation of Rehabilitation Interventions for People With Complex Psychosis

Introduction Between one fifth and one quarter of people who become unwell with a psychotic disorder will develop particularly complex problems (1). These include severe, treatment-resistant symptoms and cognitive impairments that affect motivation, organizational, and social skills. Co-existing mental, neurodevelopmental, and physical health conditions can often complicate recovery further, and up to three quarters have been found to be vulnerable to self-neglect and/or exploitation by others (2). Despite their high levels of need, this group has been missing from recent mental health policy internationally, resulting in inadequate treatment and, worryingly, increasing levels of institutionalization (3). The publication in 2020 of the first National Institute for Health and Care Excellence (NICE) Guideline on the mental health rehabilitation of adults with complex psychosis (4) is therefore a very welcome and important milestone, but there is an ongoing, urgent need for research to identify effective interventions for this group. In this Research Topic we aimed to collate relevant work that can help to address this evidence gap

Improving Health Outcomes for Patients with Depression: A Population Health Imperative. Report on an Expert Panel Meeting

Improving Health Outcomes for Patients with Depression: A Population Health Imperative. Report on an Expert Panel Meeting Janice L. Clarke, RN, Alexis Skoufalos, EdD, Alice Medalia, PhD, and A. Mark Fendrick, MD Editorial: A Call to Action: David B. Nash, MD, MBA???S-2 Overview: Depression and the Population Health Imperative???S-3 Promoting Awareness of the Issues and Opportunities for Improvement???S-5 Cognitive Dysfunction in Affective Disorders???S-5 Critical Role of Employers in Improving Health Outcomes for Employees with Depression???S-6 Closing the Behavioral Health Professional and Process Gaps???S-6 Achieving the Triple Aim for Patients with Depressive Disorders???S-6 Improving the Experience of Care for Patients with Depression???S-6 Improving Quality of Care and Health Outcomes for Patients with Depression???S-7 Changing the Cost of Care Discussion from How Much to How Well???S-8 Panel Insights and Recommendations???S-9 Conclusion???S-10Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/140195/1/pop.2016.0114.pd

Nuclear lamins: Structure and function in mechanobiology

Nuclear lamins are type V intermediate filament proteins that polymerize into complex filamentous meshworks at the nuclear periphery and in less structured forms throughout the nucleoplasm. Lamins interact with a wide range of nuclear proteins and are involved in numerous nuclear and cellular functions. Within the nucleus, they play roles in chromatin organization and gene regulation, nuclear shape, size, and mechanics, and the organization and anchorage of nuclear pore complexes. At the whole cell level, they are involved in the organization of the cytoskeleton, cell motility, and mechanotransduction. The expression of different lamin isoforms has been associated with developmental progression, differentiation, and tissue-specific functions. Mutations in lamins and their binding proteins result in over 15 distinct human diseases, referred to as laminopathies. The laminopathies include muscular (e.g., Emery-Dreifuss muscular dystrophy and dilated cardiomyopathy), neurological (e.g., microcephaly), and metabolic (e.g., familial partial lipodystrophy) disorders as well as premature aging diseases (e.g., Hutchinson-Gilford Progeria and Werner syndromes). How lamins contribute to the etiology of laminopathies is still unknown. In this review article, we summarize major recent findings on the structure, organization, and multiple functions of lamins in nuclear and more global cellular processes

Faktor-Faktor Yang Mempengaruhi Keputusan Keluarga Dalam Pemanfaatan Pelayanan Bersalin Di Rumah Sakit Dr. Asmir Salatiga

Rumah sakit Dr. Asmir Salatiga merupakan rumah sakit pemerintah yang dalam pelaksanaan diperuntukkan melayani anggota TNI. Dimanan keberadaan rumah sakit Dr. Asmir Salatiga memang diprioritaskan bagi pelayanan kesehatan bagi anggota TNI di wilayah Salatiga. Namun dalam kenyataannya tidak semua anggota TNI di wilayah Salatiga khususnya di asrama Yonif 411 Salatiga menggunakan fasilitas kesehatan yang terdapat di rumah sakit Dr. Asmir Salatiga tersebut. Hasil survey pada ibu bersalin di asrama menunjukkan menunjukkan tingkat pemanfaatan sebesar 52%. Penelitian ini bertujuan mengetahui faktorfaktor apa saja yang mempengaruhi pengambilan keputusan keluarga dalam pemanfaatan pelayanan bersalin di Rumah Sakit Dr. Asmir Salatiga sebagai tempat bersalin bagi ibu hamil di Asrama Yonif 411 Salatiga. Penelitian ini adalah deskriptif analitik dengan rancangan cross sectional. Sampel penelitian adalah sebanyak 67 responden dengan teknik total sampling. Teknik pengolahan data menggunakan teknik univariat dan bivariat menggunakan analisis chi square. Kesimpulan penelitian menunjukkan bahwa: (1) terdapat pengaruh persepsi sehat sakit tentang kehamilan dengan pemanfaatan pelayanan bersalin di rumah sakit Dr. Asmir Salatiga pada ibu bersalin di Asrama Yonif 411 Salatiga, dan (2) tidak terdapat pengaruh persepsi tentang kualitas pelayanan dengan pemanfaatan pelayanan bersalin di RS. Dr. Asmir Salatiga pada ibu bersalin di Asrama Yonif 411 Salatiga

Nanoscale resolution of microbial fiber degradation in action

The lives of microbes unfold at the micron scale, and their molecular machineries operate at the nanoscale. Their study at these resolutions is key toward achieving a better understanding of their ecology. We focus on cellulose degradation of the canonical Clostridium thermocellum system to comprehend how microbes build and use their cellulosomal machinery at these nanometer scales. Degradation of cellulose, the most abundant organic polymer on Earth, is instrumental to the global carbon cycle. We reveal that bacterial cells form 'cellulosome capsules' driven by catalytic product-dependent dynamics, which can increase the rate of hydrolysis. Biosynthesis of this energetically costly machinery and cell growth are decoupled at the single-cell level, hinting at a division-of-labor strategy through phenotypic heterogeneity. This novel observation highlights intrapopulation interactions as key to understanding rates of fiber degradation

Nucleoplasmic lamin C rapidly accumulates at sites of nuclear envelope rupture with BAF and cGAS

In mammalian cell nuclei, the nuclear lamina (NL) underlies the nuclear envelope (NE) to maintain nuclear structure. The nuclear lamins, the major structural components of the NL, are involved in the protection against NE rupture induced by mechanical stress. However, the specific role of the lamins in repair of NE ruptures has not been fully determined. Our analyses using immunofluorescence and live-cell imaging revealed that the nucleoplasmic pool of lamin C rapidly accumulated at sites of NE rupture induced by laser microirradiation in mouse embryonic fibroblasts. The accumulation of lamin C at the rupture sites required both the immunoglobulin-like fold domain that binds to barrier-to-autointegration factor (BAF) and a nuclear localization signal. The accumulation of nuclear BAF and cytoplasmic cyclic GMP-AMP synthase (cGAS) at the rupture sites was in part dependent on lamin A/C. These results suggest that nucleoplasmic lamin C, BAF, and cGAS concertedly accumulate at sites of NE rupture for rapid repair

Vimentin intermediate filaments and filamentous actin form unexpected interpenetrating networks that redefine the cell cortex

The cytoskeleton of eukaryotic cells is primarily composed of networks of filamentous proteins, F-actin, microtubules, and intermediate filaments. Interactions among the cytoskeletal components are important in determining cell structure and in regulating cell functions. For example, F-actin and microtubules work together to control cell shape and polarity, while the subcellular organization and transport of vimentin intermediate filament (VIF) networks depend on their interactions with microtubules. However, it is generally thought that F-actin and VIFs form two coexisting but separate networks that are independent due to observed differences in their spatial distribution and functions. In this paper, we present a closer investigation of both the structural and functional interplay between the F-actin and VIF cytoskeletal networks. We characterize the structure of VIFs and F-actin networks within the cell cortex using structured illumination microscopy and cryo-electron tomography. We find that VIFs and F-actin form an interpenetrating network (IPN) with interactions at multiple length scales, and VIFs are integral components of F-actin stress fibers. From measurements of recovery of cell contractility after transient stretching, we find that the IPN structure results in enhanced contractile forces and contributes to cell resilience. Studies of reconstituted networks and dynamic measurements in cells suggest direct and specific associations between VIFs and F-actin. From these results, we conclude that VIFs and F-actin work synergistically, both in their structure and in their function. These results profoundly alter our understanding of the contributions of the components of the cytoskeleton, particularly the interactions between intermediate filaments and F-actin

Characterisation of flame-generated soot and soot-in-oil using electron tomography volume reconstructions and comparison with traditional 2D-TEM measurements

This work characterises soot nanoparticles by electron tomography using Weighted Back Projection algorithm and appraises the uncertainties in two-dimensional calculations by comparison with 3D parameters for flame-generated soot and diesel soot-in-oil. Bright field TEM was used to capture 2D images of soot. Large uncertainties exist in 2D-measured morphological parameters. The flame-generated particle showed an extensive 3D structure while the soot-in-oil was notably two-dimensional. Morphological parameters of flame-generated soot and diesel soot-in-oil were different; primary particles, volume, and surface area varied significantly over the range of viewing angle, with differences as large as 60%. 2D flame-generated soot volume underestimated 3D measurements by 38%; soot-in-oil 2D and 3D-derived volumes were within 4%. 2D calculations of fractal dimension generally underestimate the 3D value