Beta cells preferentially exchange cationic molecules via connexin 36 gap junction channels

Aims/hypothesis: Pancreatic beta cells are connected by gap junction channels made of connexin 36 (Cx36), which permit intercellular exchanges of current-carrying ions (ionic coupling) and other molecules (metabolic coupling). Previous studies have suggested that ionic coupling may extend to larger regions of pancreatic islets than metabolic coupling. The aim of the present study was to investigate whether this apparent discrepancy reflects a difference in the sensitivity of the techniques used to evaluate beta cell communication or a specific characteristic of the Cx36 channels themselves. Methods: We microinjected several gap junction tracers, differing in size and charge, into individual insulin-producing cells and evaluated their intercellular exchange either within intact islets of control, knockout and transgenic mice featuring beta cells with various levels of Cx36, or in cultures of wild-type and Cx36-transfected MIN6 cells. Results: We found that (1) Cx36 channels favour the exchange of cations and larger positively charged molecules between beta cells at the expense of anionic molecules; (2) this exchange occurs across sizable portions of pancreatic islets; and (3) during glibenclamide (known as glyburide in the USA and Canada) stimulation beta cell coupling increases to an extent that varies for different gap junction-permeant molecules. Conclusions/interpretation: The data show that beta cells are extensively coupled within pancreatic islets via exchanges of mostly positively charged molecules across Cx36 channels. These exchanges selectively increase during stimulation of insulin secretion. The identification of this permselectivity is expected to facilitate the identification of endogenous permeant molecules and of the mechanism whereby Cx36 signalling significantly contributes to the modulation of insulin secretio

Systematic study of the PDC speckle structure for quantum imaging applications

Sub shot noise imaging of weak object by exploiting Parametric Down Converted light represents a very interesting technological development. A precise characterization of PDC speckle structure in dependence of pump beam parameters is a fundamental tool for this application. In this paper we present a first set of data addressed to this purpose

Quantum state reconstruction using binary data from on/off photodetection

The knowledge of the density matrix of a quantum state plays a fundamental role in several fields ranging from quantum information processing to experiments on foundations of quantum mechanics and quantum optics. Recently, a method has been suggested and implemented in order to obtain the reconstruction of the diagonal elements of the density matrix exploiting the information achievable with realistic on/off detectors, e.g. silicon avalanche photo-diodes, only able to discriminate the presence or the absence of light. The purpose of this paper is to provide an overview of the theoretical and experimental developments of the on/off method, including its extension to the reconstruction of the whole density matrix.Comment: revised version, 11 pages, 6 figures, to appear as a review paper on Adv. Science Let

Enterococcus faecalis bacteremia: please do the echo

Infective endocarditis (IE) caused by Enterococcus faecalis (E. faecalis) is a disease of the elderly with an increasing incidence, often health-care associated and with in-hospital mortality rates around 10-20%. E. faecalis IE is notoriously challenging to diagnose due to unspecific symptoms, often presenting with a complex clinical picture with low-grade fever and only moderately elevated infectious parameters. In a newly published prospective multicenter study using echocardiography to screen E. faecalis bacteremia patients, we found an IE prevalence as high as 26%. The 344 included patients with E. faecalis bacteremia had a mean age of 74 (±12) years confirming that it is indeed a disease of the elderly. The key feature of the study was that echocardiography was performed in all patients including transesophageal echocardiography (TEE) in 74%. Transthoracic echocardiography (TTE) missed vegetations in half of the cases where TEE demonstrated vegetations, underlining the importance of TEE

A Comprehensive Framework for the Security Risk Management of Cyber-Physical Systems

Cyber Physical Systems are facing huge and diverse set of security risks, especially cyber-attacks that can cause disruption to physical services or create a national disaster. Information and communication technology (ICT) has made a remarkable impact on the society. A Cyber Physical System (CPS) relies basically on information and communication technology, which puts the system\u2019s assets under certain risks especially cyber ones, and hence they must be kept under control by means of security countermeasures that generate confidence in the use of these assets. And so there is a critical need to give a great attention on the cybersecurity of these systems, which consequently leads to the safety of the physical world. This goal is achieved by adopting a solution that applies processes, plans and actions to prevent or reduce the effects of threats. Traditional IT risk assessment methods can do the job, however, and because of the characteristics of a CPS, it is more efficient to adopt a solution that is wider than a method, and addresses the type, functionalities and complexity of a CPS. This chapter proposes a framework that breaks the restriction to a traditional risk assessment method and encompasses wider set of procedures to achieve a high level strategy that could be adopted in the risk management process, in particular the cybersecurity of cyber-physical systems

Quantum and classical characterization of single/few photon detectors

This paper's purpose is to review the results recently obtained in the Quantum Optics labs of the National Institute of Metrological Research (INRIM) in the field of single- and few-photon detectors calibration, from both the classical and quantum viewpoint. In the first part of the paper is presented the calibration of a single-photon detector with absolute methods, while in the second part we focus on photon-number-resolving detectors, discussing both the classical and quantum characterization of such devices.Comment: Quantum Matter in pres

Improving resolution-sensitivity trade off in sub-shot noise quantum imaging

One of the challenges of quantum technologies is realizing the quantum advantage, predicted for ideal systems, in real applications, which have to cope with decoherence and inefficiencies. In quantum metrology, sub-shot-noise quantum imaging (SSNQI) and sensing methods can provide genuine quantum enhancement in realistic situations. However, wide-field SSNQI schemes realized so far suffer a trade-off between the resolution and the sensitivity gain over a classical counterpart: small pixels or integrating area are necessary to achieve high imaging resolution, but larger pixels allow a better detection efficiency of quantum correlations, which means a larger quantum advantage. Here, we show how the SSNQI protocol can be optimized to significantly improve the resolution without giving up the quantum advantage in sensitivity. We show a linear resolution improvement (up to a factor 3) with respect to the simple protocol used in previous demonstrations