Determination of the fate of polynuclear aromatic hydrocarbons in natural water systems

The polynuclear aromatic hydrocarbons, or PAH, are of current concern as water pollutants and potential health hazards. The presence of PAH in natural water systems was evaluated and an analytical technique for specific PAH was developed. It was found that the PAH are not soluble in water but they either are present as particulate material or as material adsorbed on solid surfaces in natural water systems. The photodecomposition of two PAH, 1,2 benzanthracene, or BA, and 3,4 benzpyrene, or BP, was examined. Both compounds decompose under ultraviolet light to form their quinones, which then further decompose. Both BP and BA decompose following first order kinetics in true solution in 20 percent acetone in water. Particulate BA also decomposes following first order reaction kinetics, a1 though particulate BP will decompose only to a depth of 0.2 pm before decomposition stops. This decomposition is relatively unaffected by water chemistry and will occur under solar radiation and in turbid waters.U.S. Department of the InteriorU.S. Geological SurveyOpe

A Note On Effects of Wounds On Heartwood Formation in White Oak (Quercus ALBA L.)

Heartwood formation was retarded by wounds on white oaks in Maine and Missouri. Maine samples with fire wounds had 22-23 rings of sapwood near the wound whereas control trees had 9. Missouri samples subjected to frill and herbicide treatments averaged 16-17 rings of sapwood near the wound, whereas control trees averaged 12. The effects of wounding on Missouri trees were pronounced at heights of 1, 2.5, and 6 m

A Note on Effects of Sewage Effluent Irrigation on Specific Gravity and Growth Rate of White and Red Oaks

A 2.5-acre forested terrace of mixed hardwoods (predominately oak) in southern Missouri was sprinkler-irrigated with treated sewage effluent. Ninety-two oak trees were sampled (increment cores) at breast height. There were 41 white oaks and 52 red oaks. Growth rate increased significantly for white oaks and specific gravity increased significantly for red oaks

A Macroscopic and Microscopic Study of Compartmentalization and Wound Closure after Mechanical Wounding of Black Walnut Trees

Compartmentalization is a concept developed to explain tree response to injury. To study this concept, uniform mechanical wounds were made in fifty black walnut trees. Each tree was wounded at two different heights, 0.5 and 1.4 m, and at two different times, fall (November 1975) and spring (March 1976). The amount of wound closure was noted after one complete growing season, as were several macro- and microscopic characteristics of compartmentalization. Wound closure and compartmentalization were separate responses. Most of the wounds were closed after a single season's growth.The eight trees with one or more open wounds were among the smallest and slowest growing trees in the study. This suggests a positive relationship between growth rate and wound closure, but statistically the relationship was not significant. Wood discoloration was the most prominent wound-related defect. Greater volumes of discolored wood were associated with fall wounds than with spring wounds. Similarly, fall and spring upper wounds were associated with larger volumes of discolored wood than their lower counterparts. Prior fertilizer treatments had no effect on wound closure or compartmentalization. The compartmentalization of wound-affected wood in black walnut agrees with the generalized model of compartmentalization of decay in trees (CODIT). The outer tangential and lateral compartment walls are the strongest, and the inner tangential and top and bottom compartment walls are the weakest and most easily overcome by invading microorganisms. The initial wood discoloration process did not appear to be associated with microorganism activity. Effective compartmentalization was positively correlated with growth rate. Some results of this study suggest that the relative ability to compartmentalize is under genetic control

Functional divergence in the role of N-linked glycosylation in smoothened signaling

The G protein-coupled receptor (GPCR) Smoothened (Smo) is the requisite signal transducer of the evolutionarily conserved Hedgehog (Hh) pathway. Although aspects of Smo signaling are conserved from Drosophila to vertebrates, significant differences have evolved. These include changes in its active sub-cellular localization, and the ability of vertebrate Smo to induce distinct G protein-dependent and independent signals in response to ligand. Whereas the canonical Smo signal to Gli transcriptional effectors occurs in a G protein-independent manner, its non-canonical signal employs Gαi. Whether vertebrate Smo can selectively bias its signal between these routes is not yet known. N-linked glycosylation is a post-translational modification that can influence GPCR trafficking, ligand responsiveness and signal output. Smo proteins in Drosophila and vertebrate systems harbor N-linked glycans, but their role in Smo signaling has not been established. Herein, we present a comprehensive analysis of Drosophila and murine Smo glycosylation that supports a functional divergence in the contribution of N-linked glycans to signaling. Of the seven predicted glycan acceptor sites in Drosophila Smo, one is essential. Loss of N-glycosylation at this site disrupted Smo trafficking and attenuated its signaling capability. In stark contrast, we found that all four predicted N-glycosylation sites on murine Smo were dispensable for proper trafficking, agonist binding and canonical signal induction. However, the under-glycosylated protein was compromised in its ability to induce a non-canonical signal through Gαi, providing for the first time evidence that Smo can bias its signal and that a post-translational modification can impact this process. As such, we postulate a profound shift in N-glycan function from affecting Smo ER exit in flies to influencing its signal output in mice

Vaccine Potential of Nipah Virus-Like Particles

Nipah virus (NiV) was first recognized in 1998 in a zoonotic disease outbreak associated with highly lethal febrile encephalitis in humans and a predominantly respiratory disease in pigs. Periodic deadly outbreaks, documentation of person-to-person transmission, and the potential of this virus as an agent of agroterror reinforce the need for effective means of therapy and prevention. In this report, we describe the vaccine potential of NiV virus-like particles (NiV VLPs) composed of three NiV proteins G, F and M. Co-expression of these proteins under optimized conditions resulted in quantifiable amounts of VLPs with many virus-like/vaccine desirable properties including some not previously described for VLPs of any paramyxovirus: The particles were fusogenic, inducing syncytia formation; PCR array analysis showed NiV VLP-induced activation of innate immune defense pathways; the surface structure of NiV VLPs imaged by cryoelectron microscopy was dense, ordered, and repetitive, and consistent with similarly derived structure of paramyxovirus measles virus. The VLPs were composed of all the three viral proteins as designed, and their intracellular processing also appeared similar to NiV virions. The size, morphology and surface composition of the VLPs were consistent with the parental virus, and importantly, they retained their antigenic potential. Finally, these particles, formulated without adjuvant, were able to induce neutralizing antibody response in Balb/c mice. These findings indicate vaccine potential of these particles and will be the basis for undertaking future protective efficacy studies in animal models of NiV disease

Disulfide bond formation is a determinant of glycosylation site usage in the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus.

Determinants of glycosylation site usage were explored by using the hemagglutinin-neuraminidase (HN) glycoprotein of the paramyxovirus Newcastle disease virus. The amino acid sequence of the HN protein, a type II glycoprotein, has six N-linked glycosylation addition sites, G1 to G6, two of which, G5 and G6, are not used for the addition of carbohydrate (L. McGinnes and T. Morrison, Virology 212:398-410, 1995). The sequence of this protein also has 13 cysteine residues in the ectodomain (C2 to C14). Mutation of either cysteine 13 or cysteine 14 resulted in the addition of another oligosaccharide chain to the protein. These cysteine residues flank the normally unused G6 glycosylation addition site, and mutation of the G6 site eliminated the extra glycosylation found in the cysteine mutants. These results suggested that failure to form an intramolecular disulfide bond resulted in the usage of a normally unused glycosylation site. This conclusion was confirmed by preventing cotranslational disulfide bond formation in cells by using dithiothreitol. Under these conditions, the wild-type protein acquired extra glycosylation, which was eliminated by mutation of the G6 site. These results suggest that localized folding events on the nascent chain, such as disulfide bond formation, which block access to the oligosaccharyl transferase are a determinant of glycosylation site usage