Toll-Interacting Protein Regulates Immune Cell Infiltration and Promotes Colitis-Associated Cancer.

Expression of Toll-interacting protein (Tollip), a potent TLR modulator, decreases in patients with inflammatory bowel diseases (IBD), whereas Tollip <sup>-/-</sup> mice are susceptible to colitis. Tollip expression was shown to be reduced in sporadic adenoma . In contrast, we found variable Tollip expression in patients with colitis-associated adenomas. In Tollip <sup>-/-</sup> mice challenged to develop colitis-associated cancer (CAC), tumor formation was significantly reduced owing to decreased mucosal proliferative and apoptotic indexes. This protection was associated with blunt inflammatory responses without significant changes in microbial composition. mRNA expression of Cd62l and Ccr5 homing receptors was reduced in colons of untreated Tollip <sup>-/-</sup> mice, whereas CD62L <sup>+</sup> CD8 <sup>+</sup> T cells accumulated in the periphery. In Tollip-deficient adenomas Ctla-4 mRNA expression and tumor-infiltrating CD4 <sup>+</sup> Foxp3 <sup>+</sup> regulatory T cell (Treg) were decreased. Our data show that protection from CAC in Tollip-deficient mice is associated with defects in lymphocyte accumulation and composition in colitis-associated adenomas

The population genetic structure of vectors and our understanding of disease epidemiology

Understanding and predicting disease epidemiology relies on clear knowledge about the basic biology of the organisms involved. Despite the key role that arthropod vectors play in disease dynamics and detailed mechanistic work on the vectorpathogen interface, little information is often available about how these populations function under natural conditions. Population genetic studies can help fill this void by providing information about the taxonomic status of species, the spatial limits of populations, and the nature of gene flow among populations. Here, I briefly review different types of population genetic structure and some recent examples of where this information has provided key elements for understanding pathogen transmission in tick-borne systems

Tiques et maladies à tiques : biologie, écologie évolutive, épidémiologie

Présentes dans tous les écosystèmes depuis des centaines de millions d'années, les tiques sont parmi les plus anciens arthropodes apparus sur Terre, exploitant leurs hôtes bien avant l'apparition de l'homme. Hématophages, elles sont responsables chez leurs hôtes d'une grande diversité de maladies, que ce soit par spoliation sanguine ou par transmission vectorielle de virus, de bactéries ou de parasites. Leur présence accrue dans l'environnement est à l'origine de nombreux cas d'encéphalites, de borrélioses de Lyme, de fièvres récurrentes, de babésioses; Ainsi, les infestations par les tiques et les maladies qu'elles transmettent constituent un véritable problème de santé pour l'homme et pour les animaux domestiques, tant en zone tropicale que tempérée. Pour dresser un état des connaissances complet sur les tiques, cet ouvrage collectif adopte une approche pluridisciplinaire. Il décrit la systématique et l'évolution, la biologie et l'écologie des tiques ainsi que les relations complexes qu'elles entretiennent avec leur hôte. Les agents de maladies infectieuses transmis, les modes de transmission, les méthodes de prévention des risques ainsi qu'un état des lieux sur la lutte contre les tiques sont également présentés

Data from: Host association influences variation at salivary protein genes in the bat ectoparasite Cimex adjunctus

Parasite-host relationships create strong selection pressures that can lead to adaptation and increasing specialization of parasites to their hosts. Even in relatively loose host-parasite relationships, such as between generalist ectoparasites and their hosts, we may observe some degree of specialization of parasite populations to one of the multiple potential hosts. Salivary proteins are used by blood-feeding ectoparasites to prevent hemostasis in the host and maximize energy intake. We investigated the influence of association with specific host species on allele frequencies of salivary protein genes in Cimex adjunctus, a generalist blood-feeding ectoparasite of bats in North America. We analysed two salivary protein genes: an apyrase, which hydrolyses ATP at the feeding site and thus inhibits platelet aggregation, and a nitrophorin, which brings nitrous oxide to the feeding site, inhibiting platelet aggregation and vasoconstriction. We observed more variation at both salivary protein genes among parasite populations associated with different host species than among populations from different spatial locations associated with the same host species. The variation in salivary protein genes among populations on different host species was also greater than expected under a neutral scenario of genetic drift and gene flow. Finally, host species was an important predictor of allelic divergence in genotypes of individual C. adjunctus at both salivary protein genes. Our results suggest differing selection pressures on these two salivary protein genes in C. adjunctus depending on the host species

'More Than Meets the Eye': cryptic diversity and contrasting patterns of host-specificity in feather mites inhabiting seabirds

Feather mites are useful models for studying speciation due to their high diversity and strong degree of host specialization. However, studies to date have focused on the evolution of higher-level mite taxa while much hidden diversity likely occurs at the level of host genera and species. In this study, we examined the diversity and evolution of feather mites infesting six sympatric seabird species from six genera, breeding in the Cape Verde archipelago. We report 32 feather mite morphospecies categorized into 10 genera and three families, of which nine correspond to new, undescribed species. Molecular data corroborated morphological species descriptions, except for two morphologically-cryptic, but genetically distinct mite lineages related to Zachvatkinia oceanodromae and Laminalloptes simplex. Using these communities, we then applied a co-structure approach to test the contribution of ectosymbiont and host factors in driving feather mite evolution. Most seabird species hosted specific and unique feather mite species, even under sympatric conditions, and in general, feather mite species exhibited strong host-driven genetic structure. However, patterns of genetic differentiation were variable. That is, some mite species are more generalist than others and mite lineages/haplotypes can be shared by related seabird species. Interestingly, host-specific mites (e.g., Zachvatkinia spp.) tend to display much higher intra-specific diversity compared to more generalist mites (e.g., Microspalax and Plicatalloptes spp.). We discuss ectosymbiont and host life-history traits that might generate these patterns, such as host dispersal and breeding behavior and/or mite spatial and trophic specialization. Our findings highlight both the vast and largely unrecognized diversity of avian feather mites on seabirds, and the intrinsic complexity of the ecological processes underlying the evolution of these ectosymbionts

'More Than Meets the Eye': cryptic diversity and contrasting patterns of host-specificity in feather mites inhabiting seabirds

Feather mites are useful models for studying speciation due to their high diversity and strong degree of host specialization. However, studies to date have focused on the evolution of higher-level mite taxa while much hidden diversity likely occurs at the level of host genera and species. In this study, we examined the diversity and evolution of feather mites infesting six sympatric seabird species from six genera, breeding in the Cape Verde archipelago. We report 32 feather mite morphospecies categorized into 10 genera and three families, of which nine correspond to new, undescribed species. Molecular data corroborated morphological species descriptions, except for two morphologically-cryptic, but genetically distinct mite lineages related to Zachvatkinia oceanodromae and Laminalloptes simplex. Using these communities, we then applied a co-structure approach to test the contribution of ectosymbiont and host factors in driving feather mite evolution. Most seabird species hosted specific and unique feather mite species, even under sympatric conditions, and in general, feather mite species exhibited strong host-driven genetic structure. However, patterns of genetic differentiation were variable. That is, some mite species are more generalist than others and mite lineages/haplotypes can be shared by related seabird species. Interestingly, host-specific mites (e.g., Zachvatkinia spp.) tend to display much higher intra-specific diversity compared to more generalist mites (e.g., Microspalax and Plicatalloptes spp.). We discuss ectosymbiont and host life-history traits that might generate these patterns, such as host dispersal and breeding behavior and/or mite spatial and trophic specialization. Our findings highlight both the vast and largely unrecognized diversity of avian feather mites on seabirds, and the intrinsic complexity of the ecological processes underlying the evolution of these ectosymbionts

A flea-induced pre-hatching maternal effect modulates tick feeding behaviour on great tit nestlings

1. A host's defence reaction against one parasite species can modulate the habitat quality for other parasites in two ways: it can provide cross-resistance against closely related species due to antigenic similarity, or it can reduce resistance to other cohabiting species, since the mounting of multiple defence reactions is more costly. 2. Here we test whether two completely unrelated parasite species can influence each other across host generations, that is, whether a hen flea-induced maternal effect known to protect great tit (Parus major) nestlings against flea infestations will also alter tick (Ixodes ricinus) feeding behaviour on nestlings. 3. We infested experimental great tit nests with hen fleas (Ceratophyllus gallinae) prior to egg-laying to induce the maternal effect, while all parasites were removed in control nests. Nestlings from the two types of nests were then cross-fostered into flea-free foster nests to produce broods that contained both, nestlings with and without the flea-induced maternal effect. Five days after hatching, we put five larval ticks on each nestling and assessed tick feeding behaviour. 4. We found that ticks feeding on nestlings with the flea-induced maternal effect detached significantly earlier than ticks feeding on controls. The result is compatible with the hypothesis of a trans-generational parasite–parasite interaction, that is, it suggests that the flea-induced maternal effect alters tick feeding behaviour and that it may protect nestlings against tick-borne diseases by reducing tick attachment times. In addition, we found that more ticks attached on male than on female nestlings, suggesting that males are more susceptible to parasites than females as shown in other vertebrates