Improved resolution in fiber bundle inline holographic microscopy using multiple illumination sources

Recent work has shown that high-quality inline holographic microscopy images can be captured through fiber imaging bundles. Speckle patterns arising from modal interference within the bundle cores can be minimized by use of a partially-coherent optical source such as an LED delivered via a multimode fiber. This allows numerical refocusing of holograms from samples at working distances of up to approximately 1 mm from the fiber bundle before the finite coherence begins to degrade the lateral resolution. However, at short working distances the lateral resolution is limited not by coherence, but by sampling effects due to core-to-core spacing in the bundle. In this article we demonstrate that multiple shifted holograms can be combined to improve the resolution by a factor of two. The shifted holograms can be rapidly acquired by sequentially firing LEDs, which are each coupled to their own, mutually offset, illumination fiber. Following a one-time calibration, resolution-enhanced images are created in real-time at an equivalent net frame rate of up to 7.5 Hz. The resolution improvement is demonstrated quantitatively using a resolution target and qualitatively using mounted biological slides. At longer working distances, beyond 0.6 mm, the improvement is reduced as resolution becomes limited by the source spatial and temporal coherence

Optical Polarimetry of the May 2022 Lunar Eclipse

The sunlight reflected from the Moon during a total lunar eclipse has been transmitted through the Earth's atmosphere on the way to the Moon. The combination of multiple scattering and inhomogeneous atmospheric characteristics during that transmission can potentially polarize that light. A similar (although much smaller) effect should also be observable from the atmosphere of a transiting exoplanet. We present the results of polarization observations during the first 15 minutes of totality of the lunar eclipse of 2022 May 16. We find degrees of polarization of 2.1 +/- 0.4 per cent in B, 1.2 +/- 0.3 per cent in V, 0.5 +/- 0.2 per cent in R and 0.2 +/- 0.2 per cent in I. Our polarization values lie in the middle of the range of those reported for previous eclipses, providing further evidence that the induced polarization can change from event to event. We found no significant polarization difference (<0.02 per cent) between a region of dark Mare and nearby bright uplands or between the lunar limb and regions closer to the disk centre due to the different angle of incidence. This further strengthens the interpretation of the polarization's origin being due to scattering in the Earth's atmosphere rather than by the lunar regolith.Comment: Accepted for publication in MNRA

Improved depth range and resolution of inline holographic microscopy through fiber imaging bundles

Inline holographic microscopy has recently been demonstrated through fiber imaging bundles, opening up the possibility of ultra-miniaturized microscopy probes. In order to minimize artefacts arising due to the multimode behaviour of the fiber bundle cores, a partially coherent light source was used: an LED coupled into a multimode fiber. However, partial coherence limits the maximum working distance between the bundle and the sample before the resolution begins to degrade. The resolution is also limited by the finite core spacing in the fiber bundle, leading to under-sampling of the finer details of the hologram. Here, we demonstrate and evaluate several techniques for improving the resolution and working range, including tailoring the source coherence and using multiple sources, demonstrating that we can achieve at least a two-fold improvement in performance

Optical Polarimetry of the May 2022 Lunar Eclipse

The sunlight reflected from the Moon during a total lunar eclipse has been transmitted through the Earth's atmosphere on the way to the Moon. The combination of multiple scattering and inhomogeneous atmospheric characteristics during that transmission can potentially polarize that light. A similar (although much smaller) effect should also be observable from the atmosphere of a transiting exoplanet. We present the results of polarization observations during the first 15 minutes of totality of the lunar eclipse of 2022 May 16. We find degrees of polarization of 2.1 +/- 0.4 per cent in B, 1.2 +/- 0.3 per cent in V, 0.5 +/- 0.2 per cent in R and 0.2 +/- 0.2 per cent in I. Our polarization values lie in the middle of the range of those reported for previous eclipses, providing further evidence that the induced polarization can change from event to event. We found no significant polarization difference (<0.02 per cent) between a region of dark Mare and nearby bright uplands or between the lunar limb and regions closer to the disk centre due to the different angle of incidence. This further strengthens the interpretation of the polarization's origin being due to scattering in the Earth's atmosphere rather than by the lunar regolith.Comment: Accepted for publication in MNRA

Refining the prediction for OJ 287 next impact flare arrival epoch

The bright blazar OJ~287 routinely parades high brightness bremsstrahlung flares which are explained as being a result of a secondary supermassive black hole (SMBH) impacting the accretion disk of a primary SMBH in a binary system. We begin by showing that these flares occur at times predicted by a simple analytical formula, based on the Kepler equation, which explains flares since 1888. The next impact flare, namely the flare number 26, is rather peculiar as it breaks the typical pattern of two impact flares per 12 year cycle. This will be the third bremsstrahlung flare of the current cycle that follows the already observed 2015 and 2019 impact flares from OJ~287. Unfortunately, astrophysical considerations make it difficult to predict the exact arrival epoch of the flare number 26. In the second part of the paper, we describe our recent OJ~287 observations. They show that the pre-flare light curve of flare number 22, observed in 2005, exhibits similar activity as the pre-flare light curve in 2022, preceding the expected flare number 26 in our model. We argue that the pre-flare activity most likely arises in the primary jet whose activity is modulated by the transit of the secondary SMBH through the accretion disk of the primary. Observing the next impact flare of OJ~287 in October 2022 will substantiate the theory of disk impacts in binary black hole systems.Comment: 16 pages, 2 figure

Demonstration of real-time resolution improvement in fiber bundle inline holographic microscopy using multiple illumination sources

The video demonstrates that resolution enhancement in fiber bundle inline holographic microscopy can be achieved during live imaging. The video shows a Python-based graphical user-interface which displays processed and refocusing holograms as they are streamed from the camera. A High Resolution USAF imaging target was imaged, initially at a distance of 140 microns from the bundle tip. The video initially shows the refocused hologram without resolution enhancement, at a frame rate of 30 fps. The exposure was set at 16 ms and kept constant throughout. Resolution enhancement is then turned on, and the resolution visibly improves. With resolution enhancement turned on, raw frames are captured at 25 fps, giving a net enhanced-resolution frame rate of 3.1 fps. In principle a rate of 7.5 fps could be achieved using the camera and the python processing code, but the overheads of the python GUI and the screen recording software resulted in lagging at faster than 3.1 fps. When the sample is translated, motion artefacts are now seen, as expected from a multi-frame approach. Finally, use of a calibration LUT to allow fast refocusing in the GUI is demonstrated. The calibration LUT was built from depths from 100 to 500 microns in steps of 20 microns. The USAF target is shown being moved away from the bundle using a translation stage, and the numerical refocusing then adjusted in the GUI to bring it back into focus, changing which calibration is used from the LUT in the process

A Bayesian reanalysis of the Standard versus Accelerated Initiation of Renal-Replacement Therapy in Acute Kidney Injury (STARRT-AKI) trial

Background Timing of initiation of kidney-replacement therapy (KRT) in critically ill patients remains controversial. The Standard versus Accelerated Initiation of Renal-Replacement Therapy in Acute Kidney Injury (STARRT-AKI) trial compared two strategies of KRT initiation (accelerated versus standard) in critically ill patients with acute kidney injury and found neutral results for 90-day all-cause mortality. Probabilistic exploration of the trial endpoints may enable greater understanding of the trial findings. We aimed to perform a reanalysis using a Bayesian framework. Methods We performed a secondary analysis of all 2927 patients randomized in multi-national STARRT-AKI trial, performed at 168 centers in 15 countries. The primary endpoint, 90-day all-cause mortality, was evaluated using hierarchical Bayesian logistic regression. A spectrum of priors includes optimistic, neutral, and pessimistic priors, along with priors informed from earlier clinical trials. Secondary endpoints (KRT-free days and hospital-free days) were assessed using zero–one inflated beta regression. Results The posterior probability of benefit comparing an accelerated versus a standard KRT initiation strategy for the primary endpoint suggested no important difference, regardless of the prior used (absolute difference of 0.13% [95% credible interval [CrI] − 3.30%; 3.40%], − 0.39% [95% CrI − 3.46%; 3.00%], and 0.64% [95% CrI − 2.53%; 3.88%] for neutral, optimistic, and pessimistic priors, respectively). There was a very low probability that the effect size was equal or larger than a consensus-defined minimal clinically important difference. Patients allocated to the accelerated strategy had a lower number of KRT-free days (median absolute difference of − 3.55 days [95% CrI − 6.38; − 0.48]), with a probability that the accelerated strategy was associated with more KRT-free days of 0.008. Hospital-free days were similar between strategies, with the accelerated strategy having a median absolute difference of 0.48 more hospital-free days (95% CrI − 1.87; 2.72) compared with the standard strategy and the probability that the accelerated strategy had more hospital-free days was 0.66. Conclusions In a Bayesian reanalysis of the STARRT-AKI trial, we found very low probability that an accelerated strategy has clinically important benefits compared with the standard strategy. Patients receiving the accelerated strategy probably have fewer days alive and KRT-free. These findings do not support the adoption of an accelerated strategy of KRT initiation

Regional Practice Variation and Outcomes in the Standard Versus Accelerated Initiation of Renal Replacement Therapy in Acute Kidney Injury (STARRT-AKI) Trial: A Post Hoc Secondary Analysis.

ObjectivesAmong patients with severe acute kidney injury (AKI) admitted to the ICU in high-income countries, regional practice variations for fluid balance (FB) management, timing, and choice of renal replacement therapy (RRT) modality may be significant.DesignSecondary post hoc analysis of the STandard vs. Accelerated initiation of Renal Replacement Therapy in Acute Kidney Injury (STARRT-AKI) trial (ClinicalTrials.gov number NCT02568722).SettingOne hundred-fifty-three ICUs in 13 countries.PatientsAltogether 2693 critically ill patients with AKI, of whom 994 were North American, 1143 European, and 556 from Australia and New Zealand (ANZ).InterventionsNone.Measurements and main resultsTotal mean FB to a maximum of 14 days was +7199 mL in North America, +5641 mL in Europe, and +2211 mL in ANZ (p p p p p p p p = 0.007).ConclusionsAmong STARRT-AKI trial centers, significant regional practice variation exists regarding FB, timing of initiation of RRT, and initial use of continuous RRT. After adjustment, such practice variation was associated with lower ICU and hospital stay and 90-day mortality among ANZ patients compared with other regions