Otitis media in the Tgif knockout mouse implicates TGFβ signalling in chronic middle ear inflammatory disease

Otitis media with effusion (OME) is the most common cause of hearing loss in children and tympanostomy to alleviate the condition remains the commonest surgical intervention in children in the developed world. Chronic and recurrent forms of OM are known to have a very significant genetic component, however, until recently little was known of the underlying genes involved. The identification of mouse models of chronic OM has indicated a role of transforming growth factor beta (TGFβ) signalling and its impact on responses to hypoxia in the inflamed middle ear. We have, therefore, investigated the role of TGFβ signalling and identified and characterized a new model of chronic OM carrying a mutation in the gene for transforming growth interacting factor 1 (Tgif1). Tgif1 homozygous mutant mice have significantly raised auditory thresholds due to a conductive deafness arising from a chronic effusion starting at around 3 weeks of age. The OM is accompanied by a significant thickening of the middle ear mucosa lining, expansion of mucin-secreting goblet cell populations and raised levels of vascular endothelial growth factor, TNF-α and IL-1β in ear fluids. We also identified downstream effects on TGFβ signalling in middle ear epithelia at the time of development of chronic OM. Both phosphorylated SMAD2 and p21 levels were lowered in the homozygous mutant, demonstrating a suppression of the TGFβ pathway. The identification and characterization of the Tgif mutant supports the role of TGFβ signalling in the development of chronic OM and provides an important candidate gene for genetic studies in the human population

Response of soil microfauna to tillage methods and cropping systems in humic nitosols of Eastern Kenya

Yellow passion fruit (Passiflora edulis var. flavicarpa) is emerging as an important high value horticultural crop in Kenya since its introduction five years ago. The fruit is gaining wide adoption in Mbeere Sub County due to its apparent adaptation to the hot arid conditions and tolerance to Fusarium wilt disease. However, the current production levels of the fruit are low due to poor water and nutrient management, erratic rainfall and poor agronomic practices. A study was conducted to assess the agronomic practices and main constraints among the farmers in Mbeere Sub County. A stratified random sampling procedure was used to collect data from 63 households from a total population of 170,950. Data was collected on household demographics, farm size, farming systems, planting materials, fertilizer use and irrigation types. This was carried out through personal interviews with members in each household responsible for management of passion fruit orchards using structured and semi-structured questionnaires. The results indicate that the majority of the farmers (65%) grow yellow passion as an intercrop with other food crops. Nevertheless, passion contributes to <10% of the proportion of the farm, though this proportion is expected to increase with expansion in production. Major findings indicate that the main source of planting materials were seedlings from KARLO (44%), use of Yaramillar (44%) as the preferred planting fertilizers and irrigation of crops three times per week (49%). In conclusion, yellow passion is a fruit with potential for increased adoption but more detailed studies are needed to clearly identify and understand water and nutrient management.Peter Mwirigi, Maina Mwangi, and Joseph Gweyi-Onyang

On-farm experimentation on conservation agriculture in maize-legume based cropping systems in Kenya: Water use efficiency and economic impacts

Conservation agriculture (CA) is a promising technology for controlling soil degradation, mitigating drought, increasing crop yield and reducing production costs. We hypothesized that adopting CA system would improve system productivity and efficiency, hence resulting in higher profits. To test the hypothesis, we designed a study to evaluate water use efficiency (WUE) and the economic benefits (yield and gross margins) of CA in the upper and lower midlands agro-ecological zones of eastern Kenya. Four tillage treatments, including farmers’ practice (residues removed), conventional tillage (residues removed) and two CA practices with residue retention (zero tillage and furrow–ridge), were laid out in 22 farmers’ fields where each farm was treated as a replicate. The results are based on four consecutive seasons farmer–researcher managed trials during the period 2010 and 2012. CA significantly improved crop yields after the first season of experimentation. Joint use of zero tillage and furrow–ridge provided higher WUE and yield advantage (25–34%) in the third and fourth seasons compared to the conventional practices. The lower midlands zone gave higher WUE values, which can be explained by the effects of water harvesting and retention for longer period on CA treatments. CA practices have increased income on average by 12% resulted from labour cost reduction and yield increment. Weeding costs for conventional tillage were USD 88 ha−1 compared to USD 24 ha−1 for herbicide application under CA. Practicing CA will certainly increase crop yields, WUE, generate more revenue and diversify risks during poor seasons. However, these benefits may not necessarily be earned in the first season, but will accrue in subsequent seasons

A systematic, genome-wide, phenotype-driven mutagenesis programme for gene function studies in the mouse

As the human genome project approaches completion, the challenge for mammalian geneticists is to develop approaches for the systematic determination of mammalian gene function. Mouse mutagenesis will be a key element of studies of gene function. Phenotype-driven approaches using the chemical mutagen ethylnitrosourea (ENU) represent a potentially efficient route for the generation of large numbers of mutant mice that can be screened for novel phenotypes. The advantage of this approach is that, in assessing gene function, no a priori assumptions are made about the genes involved in any pathway. Phenotype-driven mutagenesis is thus an effective method for the identification of novel genes and pathways. We have undertaken a genome-wide, phenotype-driven screen for dominant mutations in the mouse. We generated and screened over 26,000 mice, and recovered some 500 new mouse mutants. Our work, along with the programme reported in the accompanying paper, has led to a substantial increase in the mouse mutant resource and represents a first step towards systematic studies of gene function in mammalian genetics