Il questionario per i docenti: costruzione, implementazione, analisi. Progetto PRODID (Preparazione alla Professionalità Docente e Innovazione Didattica) - Rapporto dell'Unità di Ricerca n.3 - prima annualità [Technical Report Series, 1/2014]

The work aims to illustrate the design and the first results of a survey which involved 1849 university professors who were teaching courses at Bachelor's and Master's Degrees of University of Padova during the academic year 2012/13. The research is part of a project of improvement of the academic educational innovation and the quality of academic teaching. The data was directly obtained using a CAWI questionnaire which was distributed to the whole teaching staff, and it was linked to administrative data related to the educational offer and students’ evaluation of teaching in the same academic year. The results were summarized using some indicators which showed the diffusion of good practices of teaching, support needs, beliefs, interest and availability of the respondents. We identified sub-groups of potential participants for the qualitative phase of the research and subjects to involve in different and integrated activities directed to develop teachers’ professional competences

The Online on Job Training. The Experience of TOL@TFA in a Training Course for Teachers

The paper presents an on-line course, preparatory to training, held at the University of Padua in the initial training high school teachers. It is an internship, named TOL@TFA, made with students attending the Internship Training Active (TFA), which is the final training course of their academic career necessary, in Italy, to obtain the title for teaching in high schools. The TOL@TFA has developed 4 learning units closely connected. During the course, the students acted in remote interaction with a group of peers and with the support of a tutor skilled in technology and teaching. In this way, they prepared them self to the experience about direct and indirect internship in the school, they learned to work online, they reflected on their future professional identity, they acquires fundamental intervention tools for the internship and for their future work and they have been introduced to the knowledge of school as a system. At the end of the training activities, through a quantitative and qualitative questionnaire, the students evaluated the experience. The results demonstrate a strong appreciation both for the quality of the training both with regard to the working model adopted

Reconstruction and functional analysis of altered molecular pathways in human atherosclerotic arteries

<p>Abstract</p> <p>Background</p> <p>Atherosclerosis affects aorta, coronary, carotid, and iliac arteries most frequently than any other body vessel. There may be common molecular pathways sustaining this process. Plaque presence and diffusion is revealed by circulating factors that can mediate systemic reaction leading to plaque rupture and thrombosis.</p> <p>Results</p> <p>We used DNA microarrays and meta-analysis to study how the presence of calcified plaque modifies human coronary and carotid gene expression. We identified a series of potential human atherogenic genes that are integrated in functional networks involved in atherosclerosis. Caveolae and JAK/STAT pathways, and S100A9/S100A8 interacting proteins are certainly involved in the development of vascular disease. We found that the system of caveolae is directly connected with genes that respond to hormone receptors, and indirectly with the apoptosis pathway.</p> <p>Cytokines, chemokines and growth factors released in the blood flux were investigated in parallel. High levels of RANTES, IL-1ra, MIP-1alpha, MIP-1beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL-17, PDGF-BB, VEGF and IFN-gamma were found in plasma of atherosclerotic patients and might also be integrated in the molecular networks underlying atherosclerotic modifications of these vessels.</p> <p>Conclusion</p> <p>The pattern of cytokine and S100A9/S100A8 up-regulation characterizes atherosclerosis as a proinflammatory disorder. Activation of the JAK/STAT pathway is confirmed by the up-regulation of IL-6, STAT1, ISGF3G and IL10RA genes in coronary and carotid plaques. The functional network constructed in our research is an evidence of the central role of STAT protein and the caveolae system to contribute to preserve the plaque. Moreover, Cav-1 is involved in SMC differentiation and dyslipidemia confirming the importance of lipid homeostasis in the atherosclerotic phenotype.</p

Pastores trashumantes del Neolítico antiguo en un entorno de alta montaña: secuencia crono-cultural de la Cova de Els Trocs (San Feliú de Veri, Huesca)

This paper presents the preliminary results of the excavation of the “Els Trocs’’ Cave, developed in the context of the research project “The pathways of the Neolithic”. The careful and detailed work of excavation and recording has revealed a complex stratigraphy in which, thanks to the radiocarbon dates on short-lived samples (cereal seeds, human bones and domestic animal remains), it has been possible to distinguish four different periods of occupation inside the cave. With this information and some preliminary analyses of part of the materials (pottery, flint and faunal remains), we have documented the presence of a human group in the mountain pastures of the axial Pyrennes, at a very early stage (beginning of the last third of the Vlth millennium cal. BC), with a transhumant pastoral economy. It was a fully neolithic population with clear evidences of the use of wheat and barley, despite they did not cultivate around the close environment of this cave, where complex burial rituals had been also documented.Este trabajo presenta los resultados preliminares obtenidos en la excavación de la Cova de Els Trocs, desarrollada dentro del proyecto de investigación "Los caminos del Neolítico". La minuciosa labor de excavación y de registro ha permitido establecer una compleja secuencia estratigráfica en la que, gracias a una veintena de dataciones radiocarbónicas sobre eventos singulares de vida corta (semillas de cereal, huesos humanos y fauna doméstica), se han podido diferenciar cuatro momentos de ocupación distintos de la cueva. Con estos datos y algunos análisis, también preliminares, de parte del registro (cerámica, sílex y restos faunísticos), se ha podido definir, en una época muy temprana (inicios del último tercio del VI milenio cal. BC.), la presencia en los pastos de montaña del Pirineo axial de un grupo humano que desarrolla una actividad pastoril trashumante. Una población plenamente neolitizada, que conoce el trigo y la cebada, aunque no los cultive en el entorno de la cueva, y que, a tenor de ciertas evidencias, practica complejos rituales tanatológicos

Pastores trashumantes del Neolítico antiguo en un entorno de alta montaña: secuencia crono-cultural de la Cova de Els Trocs (San Feliú de Veri, Huesca)

This paper presents the preliminary results of the excavation of the “Els Trocs” Cave, developed in the context of the research project “The pathways of the Neolithic”. The careful and detailed work of excavation and recording has revealed a complex stratigraphy in which, thanks to the radiocarbon dates on short-lived samples (cereal seeds, human bones and domestic animal remains), it has been possible to distinguish four different periods of occupation inside the cave. With this information and some preliminary analyses of part of the materials (pottery, flint and faunal remains), we have documented the presence of a human group in the mountain pastures of the axial Pyrennes, at a very early stage (beginning of the last third of the VIth millennium cal. BC), with a transhumant pastoral economy. It was a fully neolithic population with clear evidences of the use of wheat and barley, despite they did not cultivate around the close environment of this cave, where complex burial rituals had been also documented

Los primeros pastores trashumantes de la Alta Ribagorza

Los autores utilizan argumentos arqueológicos y arqueobiológicos para plantear la hipótesis de que quienes ocuparon la Cova de Els Trocs desde el último cuarto del VI mileno calANE hasta finales del IV (ibídem, Tab. I). Eran grupos humanos poco numerosos que practicaban una economía primordialmente pastoril. Las ocupaciones de la cueva fueron recurrentes y estacionales, llegando a estos parajes a finales de primavera o principios de verano y abandonándolos a finales de septiembre, cuando los días reducen drásticamente las horas de luz. Plantean posibles procedencias y caminos de acceso a estos pastos de verano entre las cabeceras de los ríos Ésera e Isábena y defienden un tipo de actividad trashumante o trasterminante ya desde el VI milenio calANE.La línea de investigación principal de este trabajo se desarrolla en el marco del proyecto “Los Caminos del Neolítico” (HAR200909027), bajo la dirección del Dr. Manuel Rojo Guerra, concedido por la Subdirección General de Proyectos de Investigación/Dirección General de investigación y gestión del Plan Nacional de I+D+I/Secretaría de Estado de Investigación, del Ministerio de Ciencia e Innovación, y co-financiado por el Gobierno de Aragón. Del mismo modo, la parte analítica se enmarca dentro del proyecto “Origins and spread of agriculture in the western Mediterranean region” (ERC-2008-AdG 230561), coordinado por la Dra. Leonor Peña Chocarro y financiado por el European Research Council. También, buena parte de la investigación relativa al estudio antropológico y su datación, ha sido sufragada por la German Research Foundation (Al 287/14-1).Peer reviewe

The rapid spread of SARS-COV-2 Omicron variant in Italy reflected early through wastewater surveillance

The SARS-CoV-2 Omicron variant emerged in South Africa in November 2021, and has later been identified worldwide, raising serious concerns. A real-time RT-PCR assay was designed for the rapid screening of the Omicron variant, targeting characteristic mutations of the spike gene. The assay was used to test 737 sewage samples collected throughout Italy (19/21 Regions) between 11 November and 25 December 2021, with the aim of assessing the spread of the Omicron variant in the country. Positive samples were also tested with a real-time RT-PCR developed by the European Commission, Joint Research Centre (JRC), and through nested RT-PCR followed by Sanger sequencing. Overall, 115 samples tested positive for Omicron SARS-CoV-2 variant. The first occurrence was detected on 7 December, in Veneto, North Italy. Later on, the variant spread extremely fast in three weeks, with prevalence of positive wastewater samples rising from 1.0% (1/104 samples) in the week 5–11 December, to 17.5% (25/143 samples) in the week 12–18, to 65.9% (89/135 samples) in the week 19–25, in line with the increase in cases of infection with the Omicron variant observed during December in Italy. Similarly, the number of Regions/Autonomous Provinces in which the variant was detected increased fromone in the first week, to 11 in the second, and to 17 in the last one. The presence of the Omicron variant was confirmed by the JRC real-time RT-PCR in 79.1% (91/115) of the positive samples, and by Sanger sequencing in 66% (64/97) of PCR amplicons

The rapid spread of SARS-COV-2 Omicron variant in Italy reflected early through wastewater surveillance

The SARS-CoV-2 Omicron variant emerged in South Africa in November 2021, and has later been identified worldwide, raising serious concerns. A real-time RT-PCR assay was designed for the rapid screening of the Omicron variant, targeting characteristic mutations of the spike gene. The assay was used to test 737 sewage samples collected throughout Italy (19/21 Regions) between 11 November and 25 December 2021, with the aim of assessing the spread of the Omicron variant in the country. Positive samples were also tested with a real-time RT-PCR developed by the European Commission, Joint Research Centre (JRC), and through nested RT-PCR followed by Sanger sequencing. Overall, 115 samples tested positive for Omicron SARS-CoV-2 variant. The first occurrence was detected on 7 December, in Veneto, North Italy. Later on, the variant spread extremely fast in three weeks, with prevalence of positive wastewater samples rising from 1.0% (1/104 samples) in the week 5-11 December, to 17.5% (25/143 samples) in the week 12-18, to 65.9% (89/135 samples) in the week 19-25, in line with the increase in cases of infection with the Omicron variant observed during December in Italy. Similarly, the number of Regions/Autonomous Provinces in which the variant was detected increased from one in the first week, to 11 in the second, and to 17 in the last one. The presence of the Omicron variant was confirmed by the JRC real-time RT-PCR in 79.1% (91/115) of the positive samples, and by Sanger sequencing in 66% (64/97) of PCR amplicons. In conclusion, we designed an RT-qPCR assay capable to detect the Omicron variant, which can be successfully used for the purpose of wastewater-based epidemiology. We also described the history of the introduction and diffusion of the Omicron variant in the Italian population and territory, confirming the effectiveness of sewage monitoring as a powerful surveillance tool