DEVELOPMENT OF VASCULARIZED PATHOLOGICAL AND PHYSIOLOGICAL ENGINEERED TISSUES

Vascularization is still a great challenge in Tissue Engineering. First this is important to avoid mass transfer limitation in engineered tissues but also for innovative in vivo and in vitro applications. A pre-vascularized tissue can accelerate the formation of functional anastomosis with host vasculature in vivo ensuring the sustenance of the implant, its integration and long term survival. In the same time, in vitro, it can be a suitable model in view of fundamental research about physiological and pathological angiogenesis and industrial applications like drugs screening. In this work, we analyse the formation of capillary like networks into pathological and physiological tissues obtained by bottom up approach and strengthened by an endogenous extracellular matrix (ECM). The introduction describes the fundamental role of vascularization in tissue engineering and the bottom up approach that we exploit in order to obtain stroma-rich tissues. Chapter 2 retraces interesting steps of the state of art regarding 3D engineered models of pathological and physiological conditions, specifically, analyzing human breast and skin tissues and underling the role of microenvironment in tissue functionality. Chapter 3 and 4 are dedicated to the description of our work with the aim to achieve and investigate the features of vascularized pathological and physiological engineered models suitable for pharmaceutical and clinical applications. Notably, chapter 3 describes the development of vascularized breast cancer micro-tissues which are able to recapitulate tumor vascular pro-angiogenic and stromal desmoplastic reaction, and heterogeneity in cluster migration during interstitial invasion. On the other hand, chapter 4 shows how healthy micro-tissues are assembled and cultured in order to obtain vascularized dermis/skin equivalents mirroring physiological angiogenesis. Both models are featured by the presence of an active and organized stroma which is part of an interactive microenvironment and able to receive, store and release physio-pathological stimuli. We speculate that the vascularized dermis/skin model could be useful for in vivo applications in order to repair and restore damaged tissues. Furthermore, both vascularized tumor and healthy tissues are suitable research models. Thus, allowing the study of tumor progression, physio-pathological angiogenesis and providing a platform for drug screening on multicomponent systems

El federalismo y la autonomía municipal : una mirada a partir del llamado "Consenso Fiscal"

The present paper will address how the so-called "fiscal consensus", signed at the end of last year between the national Government, the provinces and the autonomous city of Buenos Aires and, as a whole, all the fiscal pacts affect Argentine federalism, autonomy of the municipalities and, consequently, our national Constitution.Fil: Mazio, Federico Ignacio. Universidad de Buenos Aires. Facultad de Derecho. Cátedra Derecho Constitucional. Buenos Aires, ArgentinaFil: Mazio, Federico Ignacio. Universidad de Buenos Aires. Facultad de Derecho. Cátedra Derecho Tributario. Buenos Aires, ArgentinaEn el presente trabajo se abordará cómo el denominado "Consenso fiscal", suscripto a finales del año pasado entre el Gobierno Nacional, las Provincias y la Ciudad Autónoma de Buenos Aires y, en su conjunto, todas las llamadas leyes convenio (Ley de Coparticipación Federal, Convenio Multilateral, Pacto Federal para el Empleo, la Producción y el Crecimiento) afectan al Federalismo argentino, a la autonomía de los municipios y, consecuentemente, a nuestra Constitución Nacional

Predicción de Corrientes de Marea Subsuperficiales en Profundidades Bajas e Intermedias

O desenvolvimento de investigações sobre dinâmica costeira exige o conhecimento das correntes de maré em diferentes profundidades oceânicas. O modelo aqui proposto permite obter velocidades de correntes de marés através de séries de medidas próximas à superfície da água. Este modelo considera o fator de viscosidades relacionado à turbulência como constante, ao mesmo tempo que introduz o efeito Coriólis. A investigação é orientada para a componente de energia máxima M2 e os resultados são comparados com as séries de medidas em diferentes profundidades. Esta comparação permite concluir pela validade do modelo para predição da direção e sentido de rotação da corrente, bem como fornece uma aceitável resposta as suas velocidades.

Estructura mareológica en norpatagonia utilizando el modelo WQMAP

El modelo hidrodinámico bidimensional WQMap, de amplio uso internacional, ha sido aplicado en el área de los tres golfos norpatagónicos (San Matías, San José y Nuevo) y aguas adyacentes de la plataforma continental argentina. Como datos de entrada al modelo indicado, se han utilizado la batimetría más actualizada y, para aplicar como condición de contorno en los bordes abiertos, la marea brindada por los modelos globales más confiables (se optó por el OSU). Después de diseñar una grilla de mucho detalle (con retículo casi cuadrado, de aproximadamente 1700 metros de lado) y de encontrar la estabilidad numérica correspondiente se seleccionaron 402 puntos distribuidos en el recinto, para los que WQMap generó, en cada uno de ellos, las series temporales de un mes de duración de la topografía (marea) y del movimiento (corriente) de las aguas. Luego, dichas series fueron analizadas con el método armónico obteniéndose los valores de las constantes (amplitud y fase) de las principales ondas tanto de la marea como de las componentes de la corriente. En particular, se analizaron dos ondas diurnas (Oj y KJ, tres semidiumas (N2, M2 y S2) más importantes energéticamente y una cuartodiuma (MJ. Se han realizado comparaciones de los resultados obtenidos con aquellos que fueron calculados a partir de datos de mediciones realizadas con anterioridad. Con las constantes armónicas de cada componente en los 402 puntos y mediante el Surfer, un programa muy adecuado para obtener óptimas distribuciones espaciales de isolíneas, se generaron, por un lado, las cartas de cotidales e isoamplitudes de las ondas de marea ya señaladas, y por otro, las correspondientes elipses de comente. Las configuraciones brindadas permiten tener una adecuada imagen de la propagación y de la amplificación de las ondas de marea en esta región del litoral marítimo patagónico.The hydrodynamic two-dimensional model, WQMap, of ample international use, has been applied in the area of the three northpatagonian gulfs (San Matías, San Jose and Nuevo) and adjacent waters of the Argentinian continental platform. About the input data to force the model, the bathymetry more updated has been used. Amplitudes and phases derived from Oregon State University (OSU) global model were used to force the model along the open boundaries. After to design a regular grid (each element almost square of approximately 1700 meters of side) and to find the numerical stability corresponding, 402 points were selected in the domain, for which WQMap generated, in each one of them, the temporary series of a month of duration of the topography (tide) and the movement (current) of waters. Then, these series were analyzed with the harmonic method, obtaining the values of the constants (amplitude and phase) of the mainwaves. In particular, two diurnal waves (Ot andKJ, and three semidiurnal waves (N2, M2 and S2) and one quarter diurnal (MJ were analyzed to characterize the hydrodynamic state. Comparisons between the obtained computational results with the values calculated from data of measurements made previously. With the harmonics constants of each component in the 402 points and by means of the Surfer, a program to obtain optimal space distributions, were generated, on the one hand, the cotidal and corange charts of the tidal waves, and, on the other hand, the corresponding tidal current ellipses. The offered configurations allow having a suitable pattern of the propagation and the amplification of the tidal waves in the northpatagonian maritime coast.Material digitalizado en SEDICI gracias a la colaboración de la Facultad de Ciencias Astronómicas y Geofísicas (UNLP).Asociación Argentina de Geofísicos y Geodesta

Propagation and amplification of tide at the Bransfield and Gerlache Straits, northwestern Antarctic Peninsula

The propagation and amplification of the tide at the Gerlache and Bransfield Straits, northwestern side of the Antarctic Peninsula, was studied by analysis of thirteen series of direct sea level measurements. Harmonic analysis was performed to obtain the amplitudes and phases of the M_2, S_2, O_1 and K_1 tidal constituents. Based on the computed harmonic constants, cotidal and corange charts of these main constituents were prepared. The M_2 cotidal lines are parallel to the coast and show predominantly southeastward propagation, except at the western side of the Trinity Peninsula, where the M_2 wave pivots to enter the Bransfield Strait from the Weddell Sea and turns southwestward. M_2 amplitudes present a southwestward decrease. S_2 cotidal lines are normal to the Antarctic Peninsula coast and show southwestward propagation. S_2 corange lines increase eastward from the Gerlache Strait to the Antarctic Strait. O_1 and K_1 cotidal lines are also normal to the Antarctic Peninsula coast. Diurnal amplitudes are alike in the study area except at the northwestern side of the Antarctic Strait where they present a maximum gradient. Amplitudes of the main tidal constituents are higher in the northwestern Weddell Sea than at the northwestern side of the peninsula. This fact explains the maximum tidal amplitudes observed at the Antarctic Strait in comparison to the Bransfield Strait

One-dimensional tidal model of the Ría San Julián, Argentina

Se implementó un modelo numérico unidimensional para sistemas de canales interconectados con el fin de simular la hidrodinámica de la marea de la ría San Julián, Argentina. En posiciones prefijadas dentro del sistema se pronosticaron la altura de la superficie libre y los caudales, utilizando un método implícito en diferencias finitas. Los resultados de la simulación numérica se compararon con mediciones de campo, y se obtuvo una correlación satisfactoria entre marea y corrientes predichas y observadas.A one-dimensional numerical model for a system of interconnected channels was implemented to simulate the tidal hydraulics of the Ría San Julián, Argentina. Free surface displacement and discharge were computed from predetermined locations throughout the system using an implicit, finite-difference technique. The results of the numerical simulation were compared with field measurements, and a satisfactory correlation was obtained between observed and predicted tides and currents.Este documento es bilingüe: español-inglésFacultad de Ciencias Naturales y Muse

Avances en el estudio de potencial de generación mareomotriz en la Bahía de San Julián utilizando turbinas sumergibles

Este trabajo presenta los avances en un estudio de la generación eléctrica en San Julián (Santa Cruz) mediante el aprovechamiento de las corrientes marinas originadas por mareas en la bahía homónima. A partir de mediciones de la velocidad del agua en distintas profundidades en la entrada de la bahía, una modelización de las corrientes por diferencias finitas y datos aportados por empresas que desarrollan un prototipo pre-comercial de turbinas sumergibles, se ha realizado una cuantificación del potencial de generación eléctrica en la zona, utilizando este recurso.This work presents the initial results of modelling, measurements and potential energy output of a small tidal stream turbine installation in the San Julián Bay in Santa Cruz, based on water speed measurements and calculations using a finite difference circulation model. Turbine parameters were estimated based on prototype tests underway in Devon, U.K., and technical information published by the companies involved.Asociación Argentina de Energías Renovables y Medio Ambiente (ASADES

Avances en el estudio de potencial de generación mareomotriz en la Bahía de San Julián utilizando turbinas sumergibles

Este trabajo presenta los avances en un estudio de la generación eléctrica en San Julián (Santa Cruz) mediante el aprovechamiento de las corrientes marinas originadas por mareas en la bahía homónima. A partir de mediciones de la velocidad del agua en distintas profundidades en la entrada de la bahía, una modelización de las corrientes por diferencias finitas y datos aportados por empresas que desarrollan un prototipo pre-comercial de turbinas sumergibles, se ha realizado una cuantificación del potencial de generación eléctrica en la zona, utilizando este recurso.This work presents the initial results of modelling, measurements and potential energy output of a small tidal stream turbine installation in the San Julián Bay in Santa Cruz, based on water speed measurements and calculations using a finite difference circulation model. Turbine parameters were estimated based on prototype tests underway in Devon, U.K., and technical information published by the companies involved.Asociación Argentina de Energías Renovables y Medio Ambiente (ASADES

Avances en el estudio de potencial de generación mareomotriz en la Bahía de San Julián utilizando turbinas sumergibles

Este trabajo presenta los avances en un estudio de la generación eléctrica en San Julián (Santa Cruz) mediante el aprovechamiento de las corrientes marinas originadas por mareas en la bahía homónima. A partir de mediciones de la velocidad del agua en distintas profundidades en la entrada de la bahía, una modelización de las corrientes por diferencias finitas y datos aportados por empresas que desarrollan un prototipo pre-comercial de turbinas sumergibles, se ha realizado una cuantificación del potencial de generación eléctrica en la zona, utilizando este recurso.This work presents the initial results of modelling, measurements and potential energy output of a small tidal stream turbine installation in the San Julián Bay in Santa Cruz, based on water speed measurements and calculations using a finite difference circulation model. Turbine parameters were estimated based on prototype tests underway in Devon, U.K., and technical information published by the companies involved.Asociación Argentina de Energías Renovables y Medio Ambiente (ASADES