496 research outputs found
Quantitative proteomic analysis of Parkin substrates in Drosophila neurons.
Parkin (PARK2) is an E3 ubiquitin ligase that is commonly mutated in Familial Parkinson's Disease (PD). In cell culture models, Parkin is recruited to acutely depolarised mitochondria by PINK1. PINK1 activates Parkin activity leading to ubiquitination of multiple proteins, which in turn promotes clearance of mitochondria by mitophagy. Many substrates have been identified using cell culture models in combination with depolarising drugs or proteasome inhibitors, but not in more physiological settings.Here we utilized the recently introduced BioUb strategy to isolate ubiquitinated proteins in flies. Following Parkin Wild-Type (WT) and Parkin Ligase dead (LD) expression we analysed by mass spectrometry and stringent bioinformatics analysis those proteins differentially ubiquitinated to provide the first survey of steady state Parkin substrates using an in vivo model. We further used an in vivo ubiquitination assay to validate one of those substrates in SH-SY5Y cells.We identified 35 proteins that are more prominently ubiquitinated following Parkin over-expression. These include several mitochondrial proteins and a number of endosomal trafficking regulators such as v-ATPase sub-units, Syx5/STX5, ALiX/PDCD6IP and Vps4. We also identified the retromer component, Vps35, another PD-associated gene that has recently been shown to interact genetically with parkin. Importantly, we validated Parkin-dependent ubiquitination of VPS35 in human neuroblastoma cells.Collectively our results provide new leads to the possible physiological functions of Parkin activity that are not overtly biased by acute mitochondrial depolarisation
Vibrational spectrum of topologically disordered systems
The topological nature of the disorder of glasses and supercooled liquids
strongly affects their high-frequency dynamics. In order to understand its main
features, we analytically studied a simple topologically disordered model,
where the particles oscillate around randomly distributed centers, interacting
through a generic pair potential. We present results of a resummation of the
perturbative expansion in the inverse particle density for the dynamic
structure factor and density of states. This gives accurate results for the
range of densities found in real systems.Comment: Completely rewritten version, accepted in Physical Review Letter
Nonequilibrium functional RG with frequency dependent vertex function: A study of the single impurity Anderson model
We investigate nonequilibrium properties of the single impurity Anderson
model by means of the functional renormalization group (fRG) within Keldysh
formalism. We present how the level broadening Gamma/2 can be used as flow
parameter for the fRG. This choice preserves important aspects of the Fermi
liquid behaviour that the model exhibits in case of particle-hole symmetry. An
approximation scheme for the Keldysh fRG is developed which accounts for the
frequency dependence of the two-particle vertex in a way similar but not
equivalent to a recently published approximation to the equilibrium Matsubara
fRG. Our method turns out to be a flexible tool for the study of weak to
intermediate on-site interactions U <= 3 Gamma. In equilibrium we find
excellent agreement with NRG results for the linear conductance at finite gate
voltage, magnetic field, and temperature. In nonequilibrium, our results for
the current agree well with TD-DMRG. For the nonlinear conductance as function
of the bias voltage, we propose reliable results at finite magnetic field and
finite temperature. Furthermore, we demonstrate the exponentially small scale
of the Kondo temperature to appear in the second order derivative of the
self-energy. We show that the approximation is, however, not able to reproduce
the scaling of the effective mass at large interactions.Comment: [v2] - minor changes throughout the text; added new Fig. 3; corrected
pert.-theory data in Figs. 10, 11; published versio
Dynamic structure factor of the Ising model with purely relaxational dynamics
We compute the dynamic structure factor for the Ising model with a purely
relaxational dynamics (model A). We perform a perturbative calculation in the
expansion, at two loops in the high-temperature phase and at one
loop in the temperature magnetic-field plane, and a Monte Carlo simulation in
the high-temperature phase. We find that the dynamic structure factor is very
well approximated by its mean-field Gaussian form up to moderately large values
of the frequency and momentum . In the region we can investigate,
, , where is the correlation
length and the zero-momentum autocorrelation time, deviations are at
most of a few percent.Comment: 21 pages, 3 figure
A new test for random number generators: Schwinger-Dyson equations for the Ising model
We use a set of Schwinger-Dyson equations for the Ising Model to check
several random number generators. For the model in two and three dimensions, it
is shown that the equations are sensitive tests of bias originated by the
random numbers. The method is almost costless in computer time when added to
any simulation.Comment: 6 pages, 3 figure
Driving current through single organic molecules
We investigate electronic transport through two types of conjugated
molecules. Mechanically controlled break-junctions are used to couple thiol
endgroups of single molecules to two gold electrodes. Current-voltage
characteristics (IVs) of the metal-molecule-metal system are observed. These
IVs reproduce the spatial symmetry of the molecules with respect to the
direction of current flow. We hereby unambigously detect an intrinsic property
of the molecule, and are able to distinguish the influence of both the molecule
and the contact to the metal electrodes on the transport properties of the
compound system.Comment: 4 pages, 5 figure
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