696 research outputs found
Hubungan Kepercayaan Diri Dengan Komunikasi Interpersonal Pada Mahasiswa Angkatan 2013 Program Studi Ilmu Keperawatan Universitas Sam Ratulagi Manado
: Background In the interpersonal communication, confidence is needed because the recognition and appreciation of communication we will have if we have the confidence..Purpose to know The Relationship Between Interpersonal Communication with Confidence for Student class 2013 at Nursing Department, Sam Ratulangi University Manado. Design is observational analytic with approach cross sectional. Population entire student class of 2013 at Nursing Department, Sam Ratulangi University, with number 109 students and Sample use purposive sampling, with number 57 students. The data collected were processed using SPSS computer assistance (Statistical Programme For Social Science) version 20 using the chi-square test at 95% significance level (ĂĄ 0.05).Result obtained value of P =0,152 more than ĂĄ = 0,05 (p 0,05).Conclusion there is not relationship between interpersonal communication with confidence for student class 2013 at Nursing Department, Sam Ratulangi University Manado. Suggestion consideration for the department as teaching materials for courses Psychiatric Nursing, and the student is expected to improve the knowledge about confidence and interpersonal communication
Unique molecular identifier-based high-resolution HLA typing and transcript quantitation using long-read sequencing
HLA typing provides essential results for stem cell and solid organ transplants, as well as providing diagnostic benefits for various rheumatology, gastroenterology, neurology, and infectious diseases. It is becoming increasingly clear that understanding the expression of patient HLA transcripts can provide additional benefits for many of these same patient groups. Our study cohort was evaluated using a long-read RNA sequencing methodology to provide rapid HLA genotyping results and normalized HLA transcript expression. Our assay used NGSEngine to determine the HLA genotyping result and normalized mRNA transcript expression using Athlon2. The assay demonstrated an excellent concordance rate of 99.7%. Similar to previous studies, for the class I loci, patients demonstrated significantly lower expression o
A conserved set of maternal genes? Insights from a molluscan transcriptome
The early animal embryo is entirely reliant on maternal gene products for a âjump-startâ that transforms a transcriptionally inactive embryo into a fully functioning zygote. Despite extensive work on model species, it has not been possible to perform a comprehensive comparison of maternally-provisioned transcripts across the Bilateria because of the absence of a suitable dataset from the Lophotrochozoa. As part of an ongoing effort to identify the maternal gene that determines left-right asymmetry in snails, we have generated transcriptome data from 1 to 2-cell and ~32-cell pond snail (Lymnaea stagnalis) embryos. Here, we compare these data to maternal transcript datasets from other bilaterian metazoan groups, including representatives of the Ecydysozoa and Deuterostomia. We found that between 5 and 10% of all L. stagnalis maternal transcripts (~300-400 genes) are also present in the equivalent arthropod (Drosophila melanogaster), nematode (Caenorhabditis elegans), urochordate (Ciona intestinalis) and chordate (Homo sapiens, Mus musculus, Danio rerio) datasets. While the majority of these conserved maternal transcripts (âCOMATsâ) have housekeeping gene functions, they are a non-random subset of all housekeeping genes, with an overrepresentation of functions associated with nucleotide binding, protein degradation and activities associated with the cell cycle. We conclude that a conserved set of maternal transcripts and their associated functions may be a necessary starting point of early development in the Bilateria. For the wider community interested in discovering conservation of gene expression in early bilaterian development, the list of putative COMATs may be useful resource
Synthesizing audio from tongue motion during speech using tagged MRI via transformer
Investigating the relationship between internal tissue point motion of the
tongue and oropharyngeal muscle deformation measured from tagged MRI and
intelligible speech can aid in advancing speech motor control theories and
developing novel treatment methods for speech related-disorders. However,
elucidating the relationship between these two sources of information is
challenging, due in part to the disparity in data structure between
spatiotemporal motion fields (i.e., 4D motion fields) and one-dimensional audio
waveforms. In this work, we present an efficient encoder-decoder translation
network for exploring the predictive information inherent in 4D motion fields
via 2D spectrograms as a surrogate of the audio data. Specifically, our encoder
is based on 3D convolutional spatial modeling and transformer-based temporal
modeling. The extracted features are processed by an asymmetric 2D convolution
decoder to generate spectrograms that correspond to 4D motion fields.
Furthermore, we incorporate a generative adversarial training approach into our
framework to further improve synthesis quality on our generated spectrograms.
We experiment on 63 paired motion field sequences and speech waveforms,
demonstrating that our framework enables the generation of clear audio
waveforms from a sequence of motion fields. Thus, our framework has the
potential to improve our understanding of the relationship between these two
modalities and inform the development of treatments for speech disorders.Comment: SPIE Medical Imaging: Deep Dive Ora
Prevalence of Multidrug-Resistant Foodborne Pathogens and Indicator Bacteria from Edible Offal and Muscle Meats in Nashville, Tennessee
This study investigated the prevalence of antimicrobial-resistant bacteria in retail edible offal and muscle meats in Nashville, Tennessee. A total of 348 retail meats (160 edible offal and 188 muscle) were analyzed for Salmonella enterica serovar, Campylobacter, Escherichia coli, E. coli O157:H7, and enterococci. Bacteria was identified using biochemical and PCR methods. Salmonella enterica serovar (4.4% and 4.3%), Campylobacter (1.9% and 1.1%), E. coli (79.4% and 89.4%), and enterococci (88.1% and 95.7%) was detected in offal and muscle meats, respectively. Chicken liver (9.7%) was most frequently contaminated with Salmonella enterica serovar, followed by ground chicken (6.9%) and chicken wings (4.2%). No Salmonella enterica serovar was detected in beef liver, beef tripe, and ground beef. The prevalence of Campylobacter was 6.9%, 2.3%, and 1.4% in beef liver, ground beef, and ground chicken, respectively. None of the meats were positive for E. coli O157:H7. Resistance of isolates was significantly (p \u3c 0.05) highest in erythromycin (98.3%; 99.1%), followed by tetracycline (94%; 98.3%), vancomycin (88.8%; 92.2%) as compared to chloramphenicol (43.1%; 53.9%), amoxicillin/clavulanic (43.5%; 45.7%), and ciprofloxacin (45.7%; 55.7%) in offal and muscle meats, respectively. Imipenem showed the lowest resistance (0%; 0.9%). A total of 41 multidrug-resistant patterns were displayed. Edible offal could be a source of antibiotic-resistant bacteria
SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion.
The secretory pathway of eukaryotic cells packages cargo proteins into COPII-coated vesicles for transport from the endoplasmic reticulum (ER) to the Golgi. We now report that complete genetic deficiency for the COPII component SEC24A is compatible with normal survival and development in the mouse, despite the fundamental role of SEC24 in COPII vesicle formation and cargo recruitment. However, these animals exhibit markedly reduced plasma cholesterol, with mutations in Apoe and Ldlr epistatic to Sec24a, suggesting a receptor-mediated lipoprotein clearance mechanism. Consistent with these data, hepatic LDLR levels are up-regulated in SEC24A-deficient cells as a consequence of specific dependence of PCSK9, a negative regulator of LDLR, on SEC24A for efficient exit from the ER. Our findings also identify partial overlap in cargo selectivity between SEC24A and SEC24B, suggesting a previously unappreciated heterogeneity in the recruitment of secretory proteins to the COPII vesicles that extends to soluble as well as trans-membrane cargoes. DOI:http://dx.doi.org/10.7554/eLife.00444.001
Speech Audio Synthesis from Tagged MRI and Non-Negative Matrix Factorization via Plastic Transformer
The tongue's intricate 3D structure, comprising localized functional units,
plays a crucial role in the production of speech. When measured using tagged
MRI, these functional units exhibit cohesive displacements and derived
quantities that facilitate the complex process of speech production.
Non-negative matrix factorization-based approaches have been shown to estimate
the functional units through motion features, yielding a set of building blocks
and a corresponding weighting map. Investigating the link between weighting
maps and speech acoustics can offer significant insights into the intricate
process of speech production. To this end, in this work, we utilize
two-dimensional spectrograms as a proxy representation, and develop an
end-to-end deep learning framework for translating weighting maps to their
corresponding audio waveforms. Our proposed plastic light transformer (PLT)
framework is based on directional product relative position bias and
single-level spatial pyramid pooling, thus enabling flexible processing of
weighting maps with variable size to fixed-size spectrograms, without input
information loss or dimension expansion. Additionally, our PLT framework
efficiently models the global correlation of wide matrix input. To improve the
realism of our generated spectrograms with relatively limited training samples,
we apply pair-wise utterance consistency with Maximum Mean Discrepancy
constraint and adversarial training. Experimental results on a dataset of 29
subjects speaking two utterances demonstrated that our framework is able to
synthesize speech audio waveforms from weighting maps, outperforming
conventional convolution and transformer models.Comment: MICCAI 2023 (Oral presentation
Unique Molecular Identifier based High-resolution HLA Typing and Transcript Quantitation using Long-Read Sequencing
HLA typing provides essential results for stem cell and solid organ transplants, as well as providing diagnostic benefits for various rheumatology, gastroenterology, neurology, and infectious diseases. It is becoming increasingly clear that understanding the expression of patient HLA transcripts can provide additional benefits for many of these same patient groups. Our study cohort was evaluated using a long-read RNA sequencing methodology to provide rapid HLA genotyping results and normalized HLA transcript expression. Our assay used NGSEngine to determine the HLA genotyping result and normalized mRNA transcript expression using Athlon2. The assay demonstrated an excellent concordance rate of 99.7%. Similar to previous studies, for the class I loci, patients demonstrated significantly lower expression of HLA-C than HLA-A and -B (MannâWhitney U, P value = 0.0065 & P value = 0.0154, respectively). In general, the expression of class II transcripts was lower than that of class I transcripts. This study demonstrates a rapid high-resolution HLA typing assay using RNA-Seq that can provide accurate HLA genotyping and HLA allele-specific transcript expression in 7 to 8 hours, a timeline short enough to perform the assay for deceased donors
Recombinant hemagglutinin proteins formulated in a novel PELC/CpG adjuvant for H7N9 subunit vaccine development
Humans infected with H7N9 avian influenza viruses can result in severe pneumonia and acute respiratory syndrome with an approximately 40% mortality rate, and there is an urgent need to develop an effective vaccine to reduce its pandemic potential. In this study, we used a novel PELC/CpG adjuvant for recombinant H7HA (rH7HA) subunit vaccine development. After immunizing BALB/c mice intramuscularly, rH7HA proteins formulated in this adjuvant instead of an alum adjuvant elicited higher IgG, hemagglutination-inhibition, and virus neutralizing antibodies in sera; induced higher numbers of H7HA-specific IFN-Îł-secreting T cells and antibody secreting cells in spleen; and provided improved protection against live virus challenges. Our results indicate that rH7HA proteins formulated in PELC/CpG adjuvant can induce potent anti-H7N9 immunity that may provide useful information for H7N9 subunit vaccine development
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