Intracellular Ca2+ release through ryanodine receptors contributes to AMPA receptor-mediated mitochondrial dysfunction and ER stress in oligodendrocytes

Overactivation of ionotropic glutamate receptors in oligodendrocytes induces cytosolic Ca2+ overload and excitotoxic death, a process that contributes to demyelination and multiple sclerosis. Excitotoxic insults cause well-characterized mitochondrial alterations and endoplasmic reticulum (ER) dysfunction, which is not fully understood. In this study, we analyzed the contribution of ER-Ca2+ release through ryanodine receptors (RyRs) and inositol triphosphate receptors (IP3Rs) to excitotoxicity in oligodendrocytes in vitro. First, we observed that oligodendrocytes express all previously characterized RyRs and IP3Rs. Blockade of Ca2+-induced Ca2+ release by TMB-8 following α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate (AMPA) receptor-mediated insults attenuated both oligodendrocyte death and cytosolic Ca2+ overload. In turn, RyR inhibition by ryanodine reduced as well the Ca2+ overload whereas IP3R inhibition was ineffective. Furthermore, AMPA-triggered mitochondrial membrane depolarization, oxidative stress and activation of caspase-3, which in all instances was diminished by RyR inhibition. In addition, we observed that AMPA induced an ER stress response as revealed by α subunit of the eukaryotic initiation factor 2α phosphorylation, overexpression of GRP chaperones and RyR-dependent cleavage of caspase-12. Finally, attenuating ER stress with salubrinal protected oligodendrocytes from AMPA excitotoxicity. Together, these results show that Ca2+ release through RyRs contributes to cytosolic Ca2+ overload, mitochondrial dysfunction, ER stress and cell death following AMPA receptor-mediated excitotoxicity in oligodendrocytes

AGN counts at 15um. XMM observations of the ELAIS-S1-5 sample

Context: The counts of galaxies and AGN in the mid infra-red (MIR) bands are important instruments for studying their cosmological evolution. However, the classic spectral line ratios techniques can become misleading when trying to properly separate AGN from starbursts or even from apparently normal galaxies. Aims: We use X-ray band observations to discriminate AGN activity in previously classified MIR-selected starburst galaxies and to derive updated AGN1 and (Compton thin) AGN2 counts at 15 um. Methods: XMM observations of the ELAIS-S1 15um sample down to flux limits ~2x10^-15 erg cm^-2 s^-1 (2-10 keV band) were used. We classified as AGN all those MIR sources with a unabsorbed 2-10 keV X-ray luminosity higher that ~10^42 erg/s. Results: We find that at least about 13(+/-6) per cent of the previously classified starburst galaxies harbor an AGN. According to these figures, we provide an updated estimate of the counts of AGN1 and (Compton thin) AGN2 at 15 um. It turns out that at least 24% of the extragalactic sources brighter than 0.6 my at 15 um are AGN (~13% contribution to the extragalactic background produced at fluxes brighter than 0.6 mJy).Comment: Accepted for publication on A&

Optimal dispatch model for PV-electrolysis plants in self-consumption regime to produce green hydrogen: a Spanish case study

The production of green hydrogen from renewable energy by means of water electrolysis is a promising approach to support energy sector decarbonization. This paper presents a techno-economic model of plants with PV sources connected to electrolysis in self-consumption regime that considers the dynamics of electrolysis systems. The model calculates the optimal hourly dispatch of the electrolysis system including the operational states (production, standby, and idle), the load factor in production, and the energy imports and exports to the electricity grid. Results indicate that the model is a useful decision support tool to operate electrolysis plants connected to PV plants in self-consumption regimes with the target of reducing hydrogen production costs

The XMM-Newton survey of the ELAIS-S1 field II: optical identifications and multiwavelength catalogue of X-ray sources

We present optical identifications and a multi-band catalogue of a sample of 478 X-ray sources in the XMM and Chandra surveys of the central 0.6 deg^2 of the ELAIS-S1 field. The optical/infrared counterpart of each X-ray source was identified using R and IRAC 3.6 um bands. This method was complemented by the precise positions obtained through Chandra observations. Approximately 94% of the counterparts are detected in the R band, while the remaining are blank fields in the optical down to R~24.5, but have a near-infrared counterpart detected by IRAC within 6 arcsec from the XMM centroid. The multi-band catalogue contains photometry in ten photometric bands (B to the MIPS 24 um). We determined redshift and classification for 237 sources (~50% of the sample) brighter than R=24. We classified 47% of the sources with spectroscopic redshift as broad-line active galactic nuclei (BL AGNs) with z=0.1-3.5, while sources without broad-lines are about 46% of the spectroscopic sample and are found up to z=2.6. We identified 11 type 2 QSOs among the sources with X/O>8, with z=0.9-2.6, high 2-10 keV luminosity (log(L2-10keV)>=43.8 erg/s) and hard X-ray colors suggesting large absorbing columns at the rest frame (logN_H up to 23.6 cm^-2). BL AGNs show on average blue optical-to-near-infrared colors, softer X-ray colors and X-ray-to-optical colors typical of optically selected AGNs. Conversely, narrow-line sources show redder optical colors, harder X-ray flux ratio and span a wider range of X-ray-to-optical colors. On average the SEDs of high-luminosity BL AGNs resemble the power-law typical of unobscured AGNs. The SEDs of NOT BL AGNs are dominated by the galaxy emission in the optical/near-infrared, and show a rise in the mid-infrared which suggests the presence of an obscured active nucleus.Comment: 15 pages, A&A accepted, affiliations correcte