Open-ended evolution to discover analogue circuits for beyond conventional applications

This is the author's accepted manuscript. The final publication is available at Springer via http://dx.doi.org/10.1007/s10710-012-9163-8. Copyright @ Springer 2012.Analogue circuits synthesised by means of open-ended evolutionary algorithms often have unconventional designs. However, these circuits are typically highly compact, and the general nature of the evolutionary search methodology allows such designs to be used in many applications. Previous work on the evolutionary design of analogue circuits has focused on circuits that lie well within analogue application domain. In contrast, our paper considers the evolution of analogue circuits that are usually synthesised in digital logic. We have developed four computational circuits, two voltage distributor circuits and a time interval metre circuit. The approach, despite its simplicity, succeeds over the design tasks owing to the employment of substructure reuse and incremental evolution. Our findings expand the range of applications that are considered suitable for evolutionary electronics

Homeodomain Interacting Protein Kinase 2 Activation Compromises Endothelial Cell Response to Laminar Flow: Protective Role of p21waf1,cip1,sdi1

BACKGROUND: In the cardiovascular system, laminar shear stress (SS) is one of the most important source of endothelial protecting signals. Physical and chemical agents, however, including ionising radiations and anticancer drugs, may injure endothelial cells determining an increase in oxidative stress and genotoxic damage. Whether the SS protective function remains intact in the presence of strong oxidants or DNA damage is currently unclear. METHODS AND RESULTS: To investigate this aspect a series of experiments were performed in which HUVEC were exposed to sub-lethal doses of the radio-mimetic compound Bleomycin (Bleo; 10 microg/ml) which generated free radicals (ROS) without significantly compromising cell survival. Remarkably, the application of a SS of 12 dyne/cm(2) did not protect endothelial cells but markedly accelerated apoptosis compared to controls kept in static culture and in the presence of Bleo. Experiments with the inducible nitric oxide synthase (iNOS) inhibitor GW274150 significantly reduced the SS-dependent apoptosis indicating that the production of NO was relevant for this effect. At molecular level, the ataxia-telangectasia-mutated (ATM) kinase, the homeodomain-interacting protein kinase-2 (HIPK2) and p53 were found activated along a pro-apoptotic signalling pathway while p21(waf1,cip1,sdi1) was prevented from its protective action. RNA interference experiments revealed that HIPK2 and p53 were both important for this process, however, only the forced expression p21(waf1,cip1,sdi1) fully restored the SS-dependent pro-survival function. CONCLUSIONS: This study provides the first evidence that, in the presence of genotoxic damage, laminar flow contributes to endothelial toxicity and death and identifies molecular targets potentially relevant in endothelial dysfunction and cardiovascular disease pathogenesis

The activity demands and physiological responses observed in professional ballet: A systematic review

The aim of this study was to systematically review research into the activity demands and physiological responses observed in professional ballet. PubMed, Web of Science, SPORTDiscus, and ProQuest were searched for original research relating to 1) the session- specific activity demands of professional ballet, 2) the general activity demands of professional ballet, 3) the immediate physiological responses to professional ballet, or 4) the delayed physiological responses to professional ballet. From an initial 7672 studies, 22 met the inclusion criteria. Methodological quality was assessed using the Mixed Methods Appraisal Tool and a modified Downs and Black Index. Professional ballet is intermittent; however, activity characteristics and intensity vary by session type and company rank. Performances involve high volumes of jumps (5.0 ± 4.9 jumps·min-1), pliés (11.7 ± 8.4 pliés·min-1), and lifts (men - 1.9 ± 3.3 lifts·min-1), which may result in near-maximal metabolic responses. Ballet classes are less metabolically intense than performance during both barre and centre ( 5 h·day-1), but half is spent at intensities below 3 METs. Evidence is mixed regarding the delayed physiological responses to professional ballet; however, metabolic and musculoskeletal adaptations are unlikely to occur from ballet alone. The mean Downs and Black score was 62%. Appraisal tools revealed that a lack of clarity regarding sampling procedures, no power calculation, and a poor quality of statistical analysis were common limitations of the included studies. Given the large working durations and high rates of jumps, pliés, and lifts, managing training loads and recovery may be a focus for strategies seeking to optimize dancer health and wellbeing. Ballet companies should provide dancers with opportunities and resources to engage in supplementary physical training. Further research is required into the physical demands of rehearsals and the longitudinal training loads undertaken by professional ballet dancers

On morphological hierarchical representations for image processing and spatial data clustering

Hierarchical data representations in the context of classi cation and data clustering were put forward during the fties. Recently, hierarchical image representations have gained renewed interest for segmentation purposes. In this paper, we briefly survey fundamental results on hierarchical clustering and then detail recent paradigms developed for the hierarchical representation of images in the framework of mathematical morphology: constrained connectivity and ultrametric watersheds. Constrained connectivity can be viewed as a way to constrain an initial hierarchy in such a way that a set of desired constraints are satis ed. The framework of ultrametric watersheds provides a generic scheme for computing any hierarchical connected clustering, in particular when such a hierarchy is constrained. The suitability of this framework for solving practical problems is illustrated with applications in remote sensing

On the equivalence between hierarchical segmentations and ultrametric watersheds

We study hierarchical segmentation in the framework of edge-weighted graphs. We define ultrametric watersheds as topological watersheds null on the minima. We prove that there exists a bijection between the set of ultrametric watersheds and the set of hierarchical segmentations. We end this paper by showing how to use the proposed framework in practice in the example of constrained connectivity; in particular it allows to compute such a hierarchy following a classical watershed-based morphological scheme, which provides an efficient algorithm to compute the whole hierarchy.Comment: 19 pages, double-colum

Dance Exposure, Individual Characteristics, and Injury Risk over Five Seasons in a Professional Ballet Company

PURPOSE: To describe the relationships between dance exposure, dancer characteristics, and injury risk across five seasons in a professional ballet company. METHODS: Dance exposure time and clinician-reported time-loss and medical attention injury data were prospectively collected from 118 professional dancers of The Royal Ballet between 2015/16 and 2019/20. Cox proportional hazards and shared frailty models were fitted to overuse and traumatic injuries; individualized robust Z-scores for 7-day and 28-day accumulated exposure, and week-to-week change in exposure, age, sex, company rank, and injury history were included as time-varying covariates. RESULTS: Across 381,710 h of exposure, 1332 medical attention and 427 time-loss injuries were observed. Positive relationships were observed between week-to-week change in exposure and overuse time-loss (+1 Z-score hazard ratio (HR): 1.27, 95% confidence interval (CI): 1.06-1.53) and medical attention injury risk (+1 Z-score HR: 1.17, 95% CI: 1.06-1.28). A negative relationship was observed between 7-day accumulated exposure and overuse medical-attention injury risk (+1 Z-score HR: 0.74, 95% CI: 0.66-0.84). Overuse time-loss injury risk was greater in soloists compared to the corps de ballet (HR: 1.47, 95% CI: 1.01-2.15), and in dancers with a higher previous injury rate (+1 injury.1000 h-1 HR: 1.06, 95% CI: 1.02-1.10). Only age was associated with traumatic time-loss (+1-year HR: 1.05, 95% CI: 1.01-1.09) or medical attention injury risk (+1-year HR: 1.04, 95% CI: 1.01-1.07). CONCLUSION: Professional ballet companies should implement training principles such as periodization and progression, particularly in the case of senior-ranking dancers, older dancers, and dancers with high rates of previous injury. These findings provide a basis for future prospective investigations into specific causal injury pathways

Synthetic microparticles conjugated with VEGF165 improve the survival of endothelial progenitor cells via microRNA-17 inhibition

Several cell-based therapies are under pre-clinical and clinical evaluation for the treatment of ischemic diseases. Poor survival and vascular engraftment rates of transplanted cells force them to work mainly via time-limited paracrine actions. Although several approaches, including the use of soluble vascular endothelial growth factor (sVEGF)-VEGF165, have been developed in the last 10 years to enhance cell survival, they showed limited efficacy. Here, we report a pro-survival approach based on VEGF-immobilized microparticles (VEGF-MPs). VEGF-MPs prolong VEGFR-2 and Akt phosphorylation in cord blood-derived late outgrowth endothelial progenitor cells (OEPCs). In vivo, OEPC aggregates containing VEGF-MPs show higher survival than those treated with sVEGF. Additionally, VEGF-MPs decrease miR-17 expression in OEPCs, thus increasing the expression of its target genes CDKN1A and ZNF652. The therapeutic effect of OEPCs is improved in vivo by inhibiting miR-17. Overall, our data show an experimental approach to improve therapeutic efficacy of proangiogenic cells for the treatment of ischemic diseases.Soluble vascular endothelial growth factor (VEGF) enhances vascular engraftment of transplanted cells but the efficacy is low. Here, the authors show that VEGF-immobilized microparticles prolong survival of endothelial progenitors in vitro and in vivo by downregulating miR17 and upregulating CDKN1A and ZNF652