Journey to Burning Man

This comic book tells the story of four friends coping from personal trauma and stress as they embark on a journey of self-discovery and healing. The storyline focuses on character development and growth as they travel to the Burning Man festival in the Black Rock Desert of Nevada. This trip, and their desire to go in the first place, reflects open-mindedness and a willingness to engage in alternative forms of medicine beneficial to surviving and thriving after illness or injury

An apodizing phase plate coronagraph for VLT/NACO

We describe a coronagraphic optic for use with CONICA at the VLT that provides suppression of diffraction from 1.8 to 7 lambda/D at 4.05 microns, an optimal wavelength for direct imaging of cool extrasolar planets. The optic is designed to provide 10 magnitudes of contrast at 0.2 arcseconds, over a D-shaped region in the image plane, without the need for any focal plane occulting mask.Comment: 9 pages, 5 figures, to appear in Proc. SPIE Vol. 773

Confirmation and characterization of the protoplanet HD100546 b - Direct evidence for gas giant planet formation at 50 au

We present the first multi-wavelength, high-contrast imaging study confirming the protoplanet embedded in the disk around the Herbig Ae/Be star HD100546. The object is detected at $L'$ ($\sim 3.8\,\mu m$) and $M'$ ($\sim 4.8\,\mu m$), but not at $K_s$ ($\sim 2.1\,\mu m$), and the emission consists of a point source component surrounded by spatially resolved emission. For the point source component we derive apparent magnitudes of $L'=13.92\pm0.10$ mag, $M'=13.33\pm0.16$ mag, and $K_s>15.43\pm0.11$ mag (3$\sigma$ limit), and a separation and position angle of $(0.457\pm0.014)"$ and $(8.4\pm1.4)^\circ$, and $(0.472\pm0.014)"$ and $(9.2\pm1.4)^\circ$ in $L'$ and $M'$, respectively. We demonstrate that the object is co-moving with HD100546 and can reject any (sub-)stellar fore-/background object. Fitting a single temperature blackbody to the observed fluxes of the point source component yields an effective temperature of $T_{eff}=932^{+193}_{-202}$ K and a radius for the emitting area of $R=6.9^{+2.7}_{-2.9}$ R$_{\rm Jupiter}$. The best-fit luminosity is $L=(2.3^{+0.6}_{-0.4})\cdot 10^{-4}\,L_{\rm Sun}$. We quantitatively compare our findings with predictions from evolutionary and atmospheric models for young, gas giant planets, discuss the possible existence of a warm, circumplanetary disk, and note that the de-projected physical separation from the host star of $(53\pm2)$ au poses a challenge standard planet formation theories. Considering the suspected existence of an additional planet orbiting at $\sim$13--14 au, HD100546 appears to be an unprecedented laboratory to study the formation of multiple gas giant planets empirically.Comment: Accepted for publication in ApJ; 13 pages incl. 9 figures, 2 tables and references in ApJ-styl

HIV-1 Nef disrupts MHC-I trafficking by recruiting AP-1 to the MHC-I cytoplasmic tail

To avoid immune recognition by cytotoxic T lymphocytes (CTLs), human immunodeficiency virus (HIV)-1 Nef disrupts the transport of major histocompatibility complex class I molecules (MHC-I) to the cell surface in HIV-infected T cells. However, the mechanism by which Nef does this is unknown. We report that Nef disrupts MHC-I trafficking by rerouting newly synthesized MHC-I from the trans-Golgi network (TGN) to lysosomal compartments for degradation. The ability of Nef to target MHC-I from the TGN to lysosomes is dependent on expression of the μ1 subunit of adaptor protein (AP) AP-1A, a cellular protein complex implicated in TGN to endolysosomal pathways. We demonstrate that in HIV-infected primary T cells, Nef promotes a physical interaction between endogenous AP-1 and MHC-I. Moreover, we present data that this interaction uses a novel AP-1 binding site that requires amino acids in the MHC-I cytoplasmic tail. In sum, our evidence suggests that binding of AP-1 to the Nef–MHC-I complex is an important step required for inhibition of antigen presentation by HIV

Seroprevalence and Risk Factors for Rickettsia and Leptospira Infection in Four Ecologically Distinct Regions of Peru.

Rickettsia and Leptospira spp. are under-recognized causes of acute febrile disease worldwide. Rickettsia species are often placed into the spotted fever group rickettsiae (SFGR) and typhus group rickettsiae (TGR). We explored the antibody prevalence among humans for these two groups of rickettsiae in four regions of Peru (Lima, Cusco, Puerto Maldonado, and Tumbes) and for Leptospira spp. in Puerto Maldonado and Tumbes. We also assessed risk factors for seropositivity and collected serum samples and ectoparasites from peri-domestic animals from households in sites with high human seroprevalence. In total, we tested 2,165 human sera for antibodies (IgG) against SFGR and TGR by ELISA and for antibodies against Leptospira by a microscopic agglutination test. Overall, human antibody prevalence across the four sites was 10.6% for SFGR (ranging from 6.2% to 14.0%, highest in Tumbes) and 3.3% for TGR (ranging from 2.6% to 6.4%, highest in Puerto Maldonado). Factors associated with seroreactivity against SFGR were male gender, older age, contact with backyard birds, and working in agriculture or with livestock. However, exposure to any kind of animal within the household decreased the odds ratio by half. Age was the only variable associated with higher TGR seroprevalence. The prevalence of Leptospira was 11.3% in Puerto Maldonado and 5.8% in Tumbes, with a borderline association with keeping animals in the household. We tested animal sera for Leptospira and conducted polymerase chain reaction (PCR) to detect Rickettsia species among ectoparasites collected from domestic animals in 63 households of seropositive participants and controls. We did not find any association between animal infection and human serostatus

Genome-scale analysis identifies paralog lethality as a vulnerability of chromosome 1p loss in cancer.

Functional redundancy shared by paralog genes may afford protection against genetic perturbations, but it can also result in genetic vulnerabilities due to mutual interdependency1-5. Here, we surveyed genome-scale short hairpin RNA and CRISPR screening data on hundreds of cancer cell lines and identified MAGOH and MAGOHB, core members of the splicing-dependent exon junction complex, as top-ranked paralog dependencies6-8. MAGOHB is the top gene dependency in cells with hemizygous MAGOH deletion, a pervasive genetic event that frequently occurs due to chromosome 1p loss. Inhibition of MAGOHB in a MAGOH-deleted context compromises viability by globally perturbing alternative splicing and RNA surveillance. Dependency on IPO13, an importin-β receptor that mediates nuclear import of the MAGOH/B-Y14 heterodimer9, is highly correlated with dependency on both MAGOH and MAGOHB. Both MAGOHB and IPO13 represent dependencies in murine xenografts with hemizygous MAGOH deletion. Our results identify MAGOH and MAGOHB as reciprocal paralog dependencies across cancer types and suggest a rationale for targeting the MAGOHB-IPO13 axis in cancers with chromosome 1p deletion