VOCALIZATION OF THE CROCODILE SKINK, TRIBOLONOTUS GRACILIS (DE ROOY, 1909), AND EVIDENCE OF PARENTAL CARE.

The crocodile or spiny skinks, Tribolonotus, comprise eight secretive semi-fossorial lizards, which are generally found under vegetation in the immediate vicinity of water (Greer and Parker, 1968; O'Shea, 1991, 1994; Rogner, 1997). Tribolonotus gracilis (Figure 1) and Tribolonotus novaeguineae are restricted to New Guinea, while the remaining six species range throughout Indonesia, Manus, New Britain, Bougainville, and the Solomon Islands (McCoy, 1980; O'Shea, 1991, 1994). Because scant life history information is available for the genus Tribolonotus, the purpose of the present contribution is to document the vocalization and parental care of T. gracilis

Development of nitrogen protecting groups and synthesis and complexation of bridged bis-tetraazamacrocycles

The development of nitrogen-protecting groups that are orthogonal to the N-benzyl group for use in cross-bridged tetraazamacrocycles and the synthesis and complexation of bridged bis-tetraazamacrocycles are presented. The allyl, p-methoxybenzyl (PMB), and 2-naphthylmethyl (Nap) nitrogen-protecting groups were investigated for use on cross-bridged tetraazamacrocycle systems and conditions for deprotection were found for the N-allyl and N-PMB protecting groups. The conditions applied for the cleavage of the N-allyl and N-PMB groups and the systems that these conditions were employed on are illustrated below. This research has demonstrated that the N-allyl and N-PMB protecting groups are applicable to a variety of systems. These protecting groups have the potential to be utilized in the synthesis of novel cross-bridged bis-tetraazamacrocycles where the N-benzyl protecting group may not be applicable. These cross-bridged systems can be further functionalized, in particular the mono-benzyl cross-bridged cyclam system 33, for a variety of biomedical purposes including positron emission tomography (PET) imaging. The N-allyl protecting group was also used to synthesize a cross-bridged bis-cyclen 31 that can now be further functionalized for a variety of biomedical purposes. Copper (II) and zinc (II) complexes of 94a and 90 and the diamide cross-bridged bis-tehaazamacrocycle 105 were also synthesized. These bis-tetraazamacrocycle systems may find use as potential anti-HIV agents or for stem-cell mobilization

Comparison of HepaRG cells following growth in proliferative and differentiated culture conditions reveals distinct bioenergetic profiles.

HepaRG is a proliferative human hepatoma-derived cell line that can be differentiated into hepatocyte-like and biliary-like cells. Differentiated HepaRG cultures maintain key hepatic functions including drug transporters and xenobiotic-metabolizing enzymes. To gain insight into proliferative and differentiated HepaRG metabolism we profiled various bioenergetic parameters and investigated cell culture levels of adenosine triphosphate (ATP), lactate, and lactate dehydrogenase (LDH) activity. Compared to differentiated-derived HepaRG, cells from proliferative cultures had increased basal and ATP-linked respiration and decreased maximal and spare respiratory capacities. Basal ATP levels but not lactate or LDH activity were increased in samples from proliferative-derived compared to differentiated-derived HepaRG. Further extracellular acidification rate (ECAR) experiments revealed parameters associated with glycolysis and oxidative phosphorylation. Under basal conditions, cells derived from both cultures had similar ECARs; however, under stressed conditions, proliferative-derived HepaRG had increases in ECAR capacity and apparent glycolytic reserve. The biguanide metformin has been reported to protect differentiated HepaRG against acetaminophen (APAP)-induced cell injury, as well as offer protection against bioenergetic deficiencies; therefore, we studied the outcome of exposure to these drugs in both culture conditions. Proliferative- and differentiated-derived cells were found to have distinct mitochondrial bioenergetic alterations when exposed to the hepatotoxic drug APAP. Metformin offered protection against loss of APAP-induced cellular viability and prevented APAP-induced decreases in bioenergetics in differentiated- but not proliferative-derived HepaRG. Distinguishingly, treatment with metformin alone reduced ATP-linked respiration, maximal respiratory capacity, and basal respiration in proliferative-derived HepaRG. Our results support that HepaRG represents an appropriate model to study drug-induced bioenergetic dysfunction

Heterogeneous Convergence

We use U.S. county-level data containing 3,058 cross-sectional observations and 41 conditioning variables to study economic growth and explore possible heterogeneity in growth determination across 32 individual states. Using a 3SLS-IV estimation method, we find that all statistically significant convergence rates (for 32 individual states) are above 2 percent, with an average of 8.1 percent. For 7 states the convergence rate can be rejected as identical to at least one other state’s convergence rate with 95 percent confidence. Convergence rates are negatively correlated with initial income. The size of government at all levels of decentralization is either unproductive or negatively correlated with growth. Educational attainment has a non-linear relationship with growth. The size of the finance, insurance and real estate, and entertainment industries are positively correlated with growth, while the size of the education industry is negatively correlated with growth. Heterogeneity in the effects of balanced growth path determinants across individual states is harder to detect than in convergence rates.Economic Growth, Conditional Convergence, County Level Data

Sigma Convergence Versus Beta Convergence: Evidence from U.S. County-Level Data

This note outlines (i) why σ-convergence may not accompany β-convergence; (ii) cites evidence of β-convergence in the U.S.; (iii) demonstrates that σ-convergence does not hold across the U.S., or within most U.S. states; and (iv) demonstrates the robustness of this finding to increases in mean income. The distributions of shocks appear important towards accounting for income disparity.σ-convergence, β-convergence, Solow growth model, speed of convergence

Heterogeneity in Convergence Rates and Income Determination across U.S. States: Evidence from County-Level Data

We utilize county-level data to explore growth determination in the U.S. and possible heterogeneity in growth determination across individual states. The data includes over 3,000 cross-sectional observations and 39 demographic control variables. We use a consistent two stage least squares estimation procedure. (We report OLS estimates as well.) The estimated convergence rate across the U.S. is about 7 percent per year – higher than the 2 percent normally found with OLS in cross-country, U.S. state, and European region samples. Estimated convergence rates for 32 individual states are above 2 percent with an average of 8.1 percent. For 29 states the convergence rate is above 2 percent with 95 percent confidence. For seven states the convergence rate can be rejected as identical to at least one other state’s convergence rate with 95 percent confidence. In examining the determinants of balanced growth path heights, we find that government at all levels of decentralization is negatively correlated with economic growth. Educational attainment of a population has a non-linear relationship with economic growth according to our estimates: growth is positively related to high-school degree attainment, seemingly unrelated to obtaining some college education, and then positively related to four-year degree or more attainment. Also, finance, insurance and real estate industry and entertainment industry are positively correlated with growth, while education industry is negatively correlated with growth. Heterogeneity in the effects of balanced growth path determinants across individual states is much harder to detect (or dismiss) than in convergence rates.Economic Growth, Income Convergence, Solow Growth Model, Balanced Growth Path, Heterogeneity in Convergence, Education and Growth, Size of Government and Growth, Consistent Estimation, County-Level Data

Nanoindentation of the a and c domains in a tetragonal BaTiO3 single crystal

Nanoindentation in conjunction with piezoresponse force microscopy was used to study domain switching and to measure the mechanical properties of individual ferroelectric domains in a tetragonal BaTiO3 single crystal. It was found that nanoindentation has induced local domain switching; the a and c domains of BaTiO3 have different elastic moduli but similar hardness. Nanoindentation modulus mapping on the a and c domains further confirmed such difference in elasticity. Finite element modeling was used to simulate the von Mises stress and plastic strain profiles of the indentations on both a and c domains, which introduces a much higher stress level than the critical value for domain nucleation