Things left unsaid: Source disclosure, the Video News Release and perceptions of credibility

Master of ScienceDepartment of Journalism and Mass CommunicationsLouise BenjaminThe video news release (VNR) has been a source of controversy since its first inclusion into newscasts in the early 1980s. This third-party (not produced by a news station) public relations and marketing-friendly content, when included alongside normally produced news stories, can make it difficult for the public to discern what is news and what is not. Problems specifically arise when news operations fail to disclose to their audience the source or provider of VNR content, and prevent news consumers from evaluating the legitimacy or intent of a VNR. A 4 (source disclosure cue: audio, video, combination of the two, and none) x 2 (source agent type: biased or neutral) experiment was implemented within this study to better understand audience evaluations, post exposure to a source disclosure cue, of the credibility of a news operation that implements VNRs within their broadcast. Disclosure cues were also evaluated for their effectiveness in raising awareness to the persuasive aspects of a VNR, and the impact of differing source agent types on participants' credibility assessment of a news operation. Results demonstrated 75% of participants (n=238) failed to correctly identify the source of the VNR when a disclosure cue was given. However, the audio and video combination condition was found to instigate the most awareness to the use of VNR. Overall, disclosure of a VNR's source could not be linked to changes in participants' evaluation of a news operation's credibility, with results demonstrating uniformly average means throughout. In addition, source disclosure could not be associated to a change in participants' awareness to the persuasive context of the VNR, with similar means exhibited. Because of the lack of an overall effect concerning credibility or knowledge of persuasive content within the study, greater media transparency is needed as are more media literacy opportunities for the public to best understand and navigate today’s complicated broadcast media reality

Country Characteristics, Internet Connectivity and Combating Misinformation: A Network Analysis of Global North-South

Analyzing data on 152 countries using network and regression analyses, this study examined how countries’ positions in the global Internet network are associated with their political, economic, and technological characteristics, and how those characteristics are related to media, information, and digital (MID) education programs in the countries. This research shows countries with higher levels of international Internet bandwidth capacity, Internet use, and press freedom status are more likely to have MID programs that are comprehensive. Differences between Global North and Global South countries were significant both in terms of Internet capacity and use and in terms of MID complexity and dimensions. MID literacy education is an important long-term solution to misinformation, as such education informs people’s epistemological beliefs which in turn have direct effects on their comprehension of various issues and topics. This study offers important scholarly and policy implications in the areas of digital connectivity, MID literacies, misinformation, and international communication. In particular, it offers guidance for comparative studies in this area

Outcomes following anastomotic leak from rectal resections, including bowel function and quality of life

Purpose Anastomotic leak (AL) is an uncommon but potentially devastating complication after rectal resection. We aim to provide an updated assessment of bowel function and quality of life after AL, as well as associated short- and long-term outcomes. Methods A retrospective audit of all rectal resections performed at a colorectal unit and associated private hospitals over the past 10 years was performed. Relevant demographic, operative, and histopathological data were collected. A prospective survey was performed regarding patients’ quality of life and fecal continence. These patients were matched with nonAL patients who completed the same survey. Results One hundred patients (out of 1,394 resections) were included. AL was contained in 66.0%, not contained in 10.0%, and only anastomotic stricture in 24.0%. Management was antibiotics only in 39.0%, percutaneous drainage in 9.0%, operative abdominal drainage in 19.0%, transrectal drainage in 6.0%, combination of percutaneous drainage and transrectal drainage in 2.0%, and combination abdominal/transrectal drainage in 1.0%. The 1-year stoma rate was 15.0%. Overall, mean Fecal Incontinence Severity Instrument scores were higher for AL patients than their matched counterparts (8.06±10.5 vs. 2.92±4.92, P=0.002). Patients with an AL had a mean EuroQol visual analogue scale (EQ-VAS) of 76.23±19.85; this was lower than the matched mean EQ-VAS for non-AL patients of 81.64±18.07, although not statistically significant (P=0.180). Conclusion The majority of AL patients in this study were managed with antibiotics only. AL was associated with higher fecal incontinence scores in the long-term; however, this did not equate to lower quality of life scores

Population genomics of domestic and wild yeasts

The natural genetics of an organism is determined by the distribution of sequences of its genome. Here we present one- to four-fold, with some deeper, coverage of the genome sequences of over seventy isolates of the domesticated baker's yeast, _Saccharomyces cerevisiae_, and its closest relative, the wild _S. paradoxus_, which has never been associated with human activity. These were collected from numerous geographic locations and sources (including wild, clinical, baking, wine, laboratory and food spoilage). These sequences provide an unprecedented view of the population structure, natural (and artificial) selection and genome evolution in these species. Variation in gene content, SNPs, indels, copy numbers and transposable elements provide insights into the evolution of different lineages. Phenotypic variation broadly correlates with global genome-wide phylogenetic relationships however there is no correlation with source. _S. paradoxus_ populations are well delineated along geographic boundaries while the variation among worldwide _S. cerevisiae_ isolates show less differentiation and is comparable to a single _S. paradoxus_ population. Rather than one or two domestication events leading to the extant baker's yeasts, the population structure of _S. cerevisiae_ shows a few well defined geographically isolated lineages and many different mosaics of these lineages, supporting the notion that human influence provided the opportunity for outbreeding and production of new combinations of pre-existing variation

The Interaction Properties of the Human Rab GTPase Family – A Comparative Analysis Reveals Determinants of Molecular Binding Selectivity

Rab GTPases constitute the largest subfamily of the Ras protein superfamily. Rab proteins regulate organelle biogenesis and transport, and display distinct binding preferences for effector and activator proteins, many of which have not been elucidated yet. The underlying molecular recognition motifs, binding partner preferences and selectivities are not well understood.Comparative analysis of the amino acid sequences and the three-dimensional electrostatic and hydrophobic molecular interaction fields of 62 human Rab proteins revealed a wide range of binding properties with large differences between some Rab proteins. This analysis assists the functional annotation of Rab proteins 12, 14, 26, 37 and 41 and provided an explanation for the shared function of Rab3 and 27. Rab7a and 7b have very different electrostatic potentials, indicating that they may bind to different effector proteins and thus, exert different functions. The subfamily V Rab GTPases which are associated with endosome differ subtly in the interaction properties of their switch regions, and this may explain exchange factor specificity and exchange kinetics.We have analysed conservation of sequence and of molecular interaction fields to cluster and annotate the human Rab proteins. The analysis of three dimensional molecular interaction fields provides detailed insight that is not available from a sequence-based approach alone. Based on our results, we predict novel functions for some Rab proteins and provide insights into their divergent functions and the determinants of their binding partner selectivity

Analyses of pig genomes provide insight into porcine demography and evolution

For 10,000 years pigs and humans have shared a close and complex relationship. From domestication to modern breeding practices, humans have shaped the genomes of domestic pigs. Here we present the assembly and analysis of the genome sequence of a female domestic Duroc pig (Sus scrofa) and a comparison with the genomes of wild and domestic pigs from Europe and Asia. Wild pigs emerged in South East Asia and subsequently spread across Eurasia. Our results reveal a deep phylogenetic split between European and Asian wild boars ∼1 million years ago, and a selective sweep analysis indicates selection on genes involved in RNA processing and regulation. Genes associated with immune response and olfaction exhibit fast evolution. Pigs have the largest repertoire of functional olfactory receptor genes, reflecting the importance of smell in this scavenging animal. The pig genome sequence provides an important resource for further improvements of this important livestock species, and our identification of many putative disease-causing variants extends the potential of the pig as a biomedical model

Going against the grain? Examining the efficacy of media literacy interventions on congenial media effects

While people’s proclivity toward congenial partisan media has been well documented, methods of resistance are less researched. This study explores the congenial media effect, the phenomenon of our gravitation to and acceptance of like-minded media, and whether a media literacy intervention can mitigate people’s acceptance of ideologically congruent partisan media. In an online experiment (N = 199), conservatives and liberals were exposed to differing media literacy interventions, one reviewing the traits of objectivity and bias, another which combined the former with an opportunity to reflect on participants’ own political beliefs. All were then exposed to amenable partisan content on the Supreme Court nomination of Brett Kavanaugh. Results demonstrated that neither intervention affected participants’ perceptions of bias toward the material, however, liberals were more critical in their evaluations, deeming the partisan content as less credible than conservatives. Additionally, for liberals exposed to the objectivity and bias intervention, lower perceptions of credibility were seen compared to liberals who received no intervention

A primer on phylogenetic generalised least squares

Phylogenetic generalised least squares (PGLS) is one of the most commonly employed phylogenetic comparative methods. The technique, a modification of generalised least squares, uses knowledge of phylogenetic relationships to produce an estimate of expected covariance in cross-species data. Closely related species are assumed to have more similar traits because of their shared ancestry and hence produce more similar residuals from the least squares regression line. By taking into account the expected covariance structure of these residuals, modified slope and intercept estimates are generated that can account for interspecific autocorrelation due to phylogeny. Here, we provide a basic conceptual background to PGLS, for those unfamiliar with the approach. We describe the requirements for a PGLS analysis and highlight the packages that can be used to implement the method. We show how phylogeny is used to calculate the expected covariance structure in the data and how this is applied to the generalised least squares regression equation. We demonstrate how PGLS can incorporate information about phylogenetic signal, the extent to which closely related species truly are similar, and how it controls for this signal appropriately, thereby negating concerns about unnecessarily 018;correcting’ for phylogeny. In addition to discussing the appropriate way to present the results of PGLS analyses, we highlight some common misconceptions about the approach and commonly encountered problems with the method. These include misunderstandings about what phylogenetic signal refers to in the context of PGLS (residuals errors, not the traits themselves), and issues associated with unknown or uncertain phylogeny