Optimizing infrared to near infrared upconversion quantum yield of β-NaYF₄:Er³⁺ in fluoropolymer matrix for photovoltaic devices

The present study reports for the first time the optimization of the infrared (1523 nm) to near-infrared (980 nm) upconversion quantum yield (UC-QY) of hexagonal trivalent erbium doped sodium yttrium fluoride (β-NaYF4:Er3+) in a perfluorocyclobutane (PFCB) host matrix under monochromatic excitation. Maximum internal and external UC-QYs of 8.4% ± 0.8% and 6.5% ± 0.7%, respectively, have been achieved for 1523 nm excitation of 970 ± 43 Wm−2 for an optimum Er3+ concentration of 25 mol% and a phosphor concentration of 84.9 w/w% in the matrix. These results correspond to normalized internal and external efficiencies of 0.86 ± 0.12 cm2 W−1 and 0.67 ± 0.10 cm2 W−1, respectively. These are the highest values ever reported for β-NaYF4:Er3+ under monochromatic excitation. The special characteristics of both the UC phosphor β-NaYF4:Er3+ and the PFCB matrix give rise to this outstanding property. Detailed power and time dependent luminescence measurements reveal energy transfer upconversion as the dominant UC mechanism

Functional interaction between the ZO-1-interacting transcription factor ZONAB/DbpA and the RNA processing factor symplekin

Epithelial tight junctions participate in the regulation of gene expression by controlling the activity of transcription factors that can interact with junctional components. One such protein is the Y-box transcription factor ZONAB/DbpA that binds to ZO-1, a component of the junctional plaque. Symplekin, another nuclear protein that can associate with tight junctions, functions in the regulation of polyadenylation and thereby promotes gene expression. Here, we addressed the question of whether these two proteins interact and whether this is of functional relevance. We demonstrate that ZONAB/DbpA and symplekin form a complex in kidney and intestinal epithelial cells that can be immunoprecipitated and that exists in the nucleus. The interaction between ZONAB/DbpA and symplekin can be reconstituted with recombinant proteins. In reporter gene assays in which ZONAB/DbpA functions as a repressor, symplekin functionally interacts with ZONAB/DbpA, indicating that symplekin can also promote transcriptional repression. RNAi experiments indicate that symplekin depletion reduces the nuclear accumulation and the transcriptional activity of ZONAB/DbpA in colon adenocarcinoma cells, resulting in inhibition of proliferation and reduced expression of the ZONAB/DbpA-target gene cyclin D1. Our data thus indicate that symplekin and ZONAB/DbpA cooperate in the regulation of transcription, and that they promote epithelial proliferation and cyclin D1 expression

Revealing the Anatomy of Vote Trading

Cooperation in the form of vote trading, also known as logrolling,is central for law-making processes, shaping the development of democratic societies. Empirical evidence of logrolling is scarce and limited to highly specific situations because existing methods are not easily applicable to broader contexts. We have developed a general and scalable methodology for revealing a network of vote traders, allowing us to measure logrolling on a large scale. Analysis on more than 9 million votes spanning 40 years in the U.S. Congress reveals a higher logrolling prevalence in the Senate and an overall decreasing trend over recent congresses, coincidental with high levels of political polarization. Our method is applicable in multiple contexts, shedding light on many aspects of logrolling and opening new doors in the study of hidden cooperation

Uncovering Vote Trading Through Networks and Computation

We develop a new methodological framework for the empirical study of legislative vote trading. Building on the concept of reciprocity in directed weighted networks, our method facilitates the measurement of vote trading on a large scale, while estimating the micro-structure of trades between individual legislators. In principle, it can be applied to a broad variety of voting data and refined for various specific contexts. It allows, for example, to study how vote trading in a specific legislative assembly varies over time. We validate our method with a computational model in which we control the level of vote trading. Finally, we demonstrate our framework in an analysis of four decades of roll call voting in the U.S. Congress

The polarized expression of Na+,K+-ATPase in epithelia depends on the association between beta-subunits located in neighboring cells

The polarized distribution of Na+,K+-ATPase plays a paramount physiological role, because either directly or through coupling with co- and countertransporters, it is responsible for the net movement of, for example, glucose, amino acids, Ca2+, K+, Cl-, and CO3H- across the whole epithelium. We report here that the beta-subunit is a key factor in the polarized distribution of this enzyme. 1) Madin-Darby canine kidney (MDCK) cells (epithelial from dog kidney) express the Na+,K+-ATPase over the lateral side, but not on the basal and apical domains, as if the contact with a neighboring cell were crucial for the specific membrane location of this enzyme. 2) MDCK cells cocultured with other epithelial types (derived from human, cat, dog, pig, monkey, rabbit, mouse, hamster, and rat) express the enzyme in all (100%) homotypic MDCK/MDCK borders but rarely in heterotypic ones. 3) Although MDCK cells never express Na+,K+-ATPase at contacts with Chinese hamster ovary (CHO) cells, they do when CHO cells are transfected with beta(1)-subunit from the dog kidney (CHO-beta). 4) This may be attributed to the adhesive property of the beta(1)-subunit, because an aggregation assay using CHO (mock-transfected) and CHO-beta cells shows that the expression of dog beta(1)-subunit in the plasma membrane does increase adhesiveness. 5) This adhesiveness does not involve adherens or tight junctions. 6) Transfection of beta(1)-subunit forces CHO-beta cells to coexpress endogenous a-subunit. Together, our results indicate that MDCK cells express Na+,K+-ATPase at a given border provided the contacting cell expresses the dog P,-subunit. The cell-cell interaction thus established would suffice to account for the polarized expression and positioning of Na+,K+-ATPase in epithelial cells

Dog Ecology and Dog Rabies Control

Dog populations, like other populations, depend on the availability of resources (food, water, and shelter). Humans either make available or deliberately withhold resources for varying proportions of dog populations. Dog-keeping practices and the duties of responsible ownership vary with the cultural setting. Dog populations often attain densities that allow the species to be a main host of rabies. The epidemiology of dog rabies is not well understood, despite the easy access to dog populations. Today dog rabies is predomina~t in developing countries. In addition to the high rate of exposure of humans to dogs, tradItional medical beliefs and practices are the most important cultural factors that lead to high numbers of cases of human rabies. Dog rabies control programs have been succe~sful in the past, but most are failing today. Program development should follow managenal principles and take into consideration the biology of dog populations as w~ll as. cultural constraints. Elimination of stray dogs IS not an effIcIent means of controllIng eIther the dog population or rabies, but it may create public awarenes

Inner disk clearing around the Herbig Ae star HD\,139614: Evidence for a planet-induced gap ?

Spatially resolving the inner dust cavity of the transitional disks is a key to understanding the connection between planetary formation and disk dispersal. The disk around the Herbig star HD 139614 is of particular interest since it presents a pretransitional nature with an au-sized gap, in the dust, that was spatially resolved by mid-IR interferometry. Using new NIR interferometric observations, we aim to characterize the 0.1-10~au region of the HD~139614 disk further and identify viable mechanisms for the inner disk clearing. We report the first multiwavelength radiative transfer modeling of the interferometric data acquired on HD~139614 with PIONIER, AMBER, and MIDI, complemented by Herschel/PACS photometries. We confirm a gap structure in the um-sized dust, extending from about 2.5 au to 6 au, and constrained the properties of the inner dust component: e.g., a radially increasing surface density profile, and a depletion of 10^3 relative to the outer disk. Since self-shadowing and photoevaporation appears unlikely to be responsible for the au-sized gap of HD~139614, we thus tested if dynamical clearing could be a viable mechanism using hydrodynamical simulations to predict the gaseous disk structure. Indeed, a narrow au-sized gap is expected when a single giant planet interacts with the disk. Assuming that small dust grains are well coupled to the gas, we found that a ~ 3~Mjup planet located at 4.5 au from the star could, in less than 1 Myr, reproduce most of the aspects of the dust surface density profile, while no significant depletion in gas occurred in the inner disk, in contrast to the dust. However, the dust-depleted inner disk could be explained by the expected dust filtration by the gap and the efficient dust growth/fragmentation in the inner disk regions. Our results support the hypothesis of a giant planet opening a gap and shaping the inner region of the HD~139614 disk.Comment: Version accepted in A&A, with typos corrections in the tex

Dbl3 drives Cdc42 signaling at the apical margin to regulate junction position and apical differentiation

Epithelial cells develop morphologically characteristic apical domains that are bordered by tight junctions, the apical–lateral border. Cdc42 and its effector complex Par6–atypical protein kinase c (aPKC) regulate multiple steps during epithelial differentiation, but the mechanisms that mediate process-specific activation of Cdc42 to drive apical morphogenesis and activate the transition from junction formation to apical differentiation are poorly understood. Using a small interfering RNA screen, we identify Dbl3 as a guanine nucleotide exchange factor that is recruited by ezrin to the apical membrane, that is enriched at a marginal zone apical to tight junctions, and that drives spatially restricted Cdc42 activation, promoting apical differentiation. Dbl3 depletion did not affect junction formation but did affect epithelial morphogenesis and brush border formation. Conversely, expression of active Dbl3 drove process-specific activation of the Par6–aPKC pathway, stimulating the transition from junction formation to apical differentiation and domain expansion, as well as the positioning of tight junctions. Thus, Dbl3 drives Cdc42 signaling at the apical margin to regulate morphogenesis, apical–lateral border positioning, and apical differentiation