LASER-VISUAL-INERTIAL ODOMETRY BASED SOLUTION FOR 3D HERITAGE MODELING: THE SANCTUARY OF THE BLESSED VIRGIN OF TROMPONE

Abstract. The advent of new mobile mapping systems that integrate different sensors has made it easier to acquire multiple 3D information with high speed. Today, technological development has allowed the creation of portable systems particularly suitable for indoor surveys, which mainly integrating LiDAR devices, chambers and inertial platforms, make it possible to create in a fast and easy way, full 3D model of the environment. However, the performance of these instruments differs depending on the acquisition context (indoor and outdoor), the characteristics of the scene (for example lighting, the presence of objects and people, reflecting surfaces, textures) and, above all, the mapping and localization algorithms implemented in devices. The purpose of this study is to analyse the results, and their accuracy, deriving from a survey conducted with the KAARTA Stencil 2 handheld system. This instrument, composed of a 3D LiDAR Velodyne VLP-16, a MEMS inertial platform and a feature tracker camera, it is able to realize the temporal 3D map of the environment. Specifically, the acquisition tests were carried out in a context of metrical documentation of an architectural heritage, in order extract architectural detail for the future reconstruction of virtual and augmented reality environments and for Historical Building Information Modeling purposes. The achieved results were analysed and the discrepancies from some reference LiDAR data are computed for a final evaluation. The system was tested in the church and cloister of the Sanctuary of the Beata Vergine del Trompone in Moncrivello (VC) (Italy).</p

LASER-VISUAL-INERTIAL ODOMETRY BASED SOLUTION for 3D HERITAGE MODELING: The SANCTUARY of the BLESSED VIRGIN of TROMPONE

The advent of new mobile mapping systems that integrate different sensors has made it easier to acquire multiple 3D information with high speed. Today, technological development has allowed the creation of portable systems particularly suitable for indoor surveys, which mainly integrating LiDAR devices, chambers and inertial platforms, make it possible to create in a fast and easy way, full 3D model of the environment. However, the performance of these instruments differs depending on the acquisition context (indoor and outdoor), the characteristics of the scene (for example lighting, the presence of objects and people, reflecting surfaces, textures) and, above all, the mapping and localization algorithms implemented in devices. The purpose of this study is to analyse the results, and their accuracy, deriving from a survey conducted with the KAARTA Stencil 2 handheld system. This instrument, composed of a 3D LiDAR Velodyne VLP-16, a MEMS inertial platform and a feature tracker camera, it is able to realize the temporal 3D map of the environment. Specifically, the acquisition tests were carried out in a context of metrical documentation of an architectural heritage, in order extract architectural detail for the future reconstruction of virtual and augmented reality environments and for Historical Building Information Modeling purposes. The achieved results were analysed and the discrepancies from some reference LiDAR data are computed for a final evaluation. The system was tested in the church and cloister of the Sanctuary of the Beata Vergine del Trompone in Moncrivello (VC) (Italy)

PO-519 Combined inhibition of MEK and PI3KCA pathway induces synergic antitumor activity in HER2 amplified colorectal cancer models

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HANDHELD VOLUMETRIC SCANNER for 3D PRINTED INTEGRATIONS of HISTORICAL ELEMENTS: COMPARISON and RESULTS

The latest technologies in the field of geomatics and additive manufacturing can provide a significant support to the restoration and conservation activities of Cultural Heritage (CH). In particular, the recent developments for both the 3D scanning techniques and the 3D printing systems are able to speed up the survey and the reconstruction of historical fragments gone lost. This research compares different meshes, obtained with different instruments and techniques, in particular a medium and a short- range volumetric handheld camera, with the aim of evaluating the best solution for the 3D printing and provide some guidelines for this kind of operation. Therefore, the focus is mainly on identifying the most effective solution to describe, represent and subsequently model small architectural details in the most automatic way, in order to step from the survey to the final printed result in the shortest time possible. Moreover, an attempt to integrate and complete not only sculptural details, by now well stated in the state of art, but also architectural elements, respecting the principles of reversibility and material recognisability typical of the modern restoration theories, has been done. The case study examined is an historical wooden gilded door with the lack of some decorative parts, in a church in the centre of Turin (Italy), symbol of the baroque architecture and damaged by a fire occurred during the Second World War

A dynamic link between H/ACA snoRNP components and cytoplasmic stress granules

Many cell stressors block protein translation, inducing formation of cytoplasmic aggregates. These aggregates, named stress granules (SGs), are composed by translationally stalled ribonucleoproteins and their assembly strongly contributes to cell survival. Composition and dynamics of SGs are thus important starting points for identifying critical factors of the stress response. In the present study we link components of the H/ACA snoRNP complexes, highly concentrated in the nucleoli and the Cajal bodies, to SG composition. H/ACA snoRNPs are composed by a core of four highly conserved proteins -dyskerin, Nhp2, Nop10 and Gar1- and are involved in several fundamental processes, including ribosome biogenesis, RNA pseudouridylation, stabilization of small nucleolar RNAs and telomere maintenance. By taking advantage of cells overexpressing a dyskerin splice variant undergoing a dynamic intracellular trafficking, we were able to show that H/ACA snoRNP components can participate in SG formation, this way contributing to the stress response and perhaps transducing signals from the nucleus to the cytoplasm. Collectively, our results show for the first time that H/ACA snoRNP proteins can have additional non-nuclear functions, either independently or interacting with each other, thus further strengthening the close relationship linking nucleolus to SG composition

Does the term 'trophic' actually mean anti-amyloidogenic? The case of NGF.

The term trophic is widely used to indicate a general pro-survival action exerted on target cells by different classes of extracellular messengers, including neurotrophins (NTs), a family of low-molecular-weight proteins whose archetypal member is the nerve growth factor (NGF). The pro-survival action exerted by NTs results from a coordinated activation of multiple metabolic pathways, some of which have only recently come to light. NGF has been shown to exert a number of different, experimentally distinguishable effects on neurons, such as survival, differentiation of target neurons, growth of nerve fibers and their guidance (tropism) toward the source of its production. We have proposed a more complete definition of the NGF trophic action that should also include its newly discovered property of inhibiting the amyloidogenic processing of amyloid precursor protein (APP), which is among the first hypothesized primary trigger of Alzheimer's disease (AD) pathogenesis. This inhibitory action appears to be mediated by a complex series of molecular events and by interactions among NGF receptors (TrkA and p75), APP processing and tau metabolic fate and fun

Tyr682 in the Aβ-precursor protein intracellular domain regulates synaptic connectivity, cholinergic function, and cognitive performance.

Processing of Aβ-precursor protein (APP) plays an important role in Alzheimer's disease (AD) pathogenesis. The APP intracellular domain contains residues important in regulating APP function and processing, in particular the 682YENPTY687 motif. To dissect the functions of this sequence in vivo, we created an APP knock-in allele mutating Y682 to Gly (APP(YG/YG) mice). This mutation alters the processing of APP and TrkA signaling and leads to postnatal lethality and neuromuscular synapse defects when expressed on an APP-like protein 2 KO background. This evidence prompted us to characterize further the APP(YG/YG) mice. Here, we show that APP(YG/YG) mice develop aging-dependent decline in cognitive and neuromuscular functions, a progressive reduction in dendritic spines, cholinergic tone, and TrkA levels in brain regions governing cognitive and motor functions. These data are consistent with our previous findings linking NGF and APP signaling and suggest a causal relationship between altered synaptic connectivity, cholinergic tone depression and TrkA signaling deficit, and cognitive and neuromuscular decline in APP(YG/YG) mice. The profound deficits caused by the Y682 mutation underscore the biological importance of APP and indicate that APP(YG/YG) are a valuable mouse model to study APP functions in physiological and pathological processes

Exercise promotes angiogenesis and improves beta-adrenergic receptor signalling in the post-ischaemic failing rat heart.

We investigated whether exercise training could promote angiogenesis and improve blood perfusion and left ventricular (LV) remodelling of the post-myocardial infarction (MI) failing heart. We also explored the contribution of ameliorated beta-adrenergic receptor signalling and function on the overall improvement of cardiac contractility reserve induced by exercise.Adult Wistar male rats were randomly assigned to one of four experimental groups. Sham-operated and post-MI heart failure (HF) rats were housed under sedentary conditions or assigned to 10-weeks of a treadmill exercise protocol. At 4 weeks after MI, sedentary HF rats showed LV eccentric hypertrophy, marked increase of LV diameters associated with severely impaired fractional shortening (14 +/- 5\%), increased LV end diastolic pressure (20.9 +/- 2.6 mmHg), and pulmonary congestion. In addition, cardiac contractile responses to adrenergic stimulation were significantly blunted. In trained HF rats, exercise was able to (i) reactivate the cardiac vascular endothelial growth factor pathway with a concurrent enhancement of myocardial angiogenesis, (ii) significantly increase myocardial perfusion and coronary reserve, (iii) reduce cardiac diameters, and (iv) improve LV contractility in response to adrenergic stimulation. This latter finding was also associated with a significant improvement of cardiac beta-adrenergic receptor downregulation and desensitization.Our data indicate that exercise favourably affects angiogenesis and improves LV remodelling and contractility reserve in a rat model of severe chronic HF

Vulnerability to low-dose combination of irinotecan and niraparib in ATM-mutated colorectal cancer

Background: Despite the advancements in new therapies for colorectal cancer (CRC), chemotherapy still constitutes the mainstay of the medical treatment. For this reason, new strategies to increase the efficacy of chemotherapy are desirable. Poly-ADP-Ribose Polymerase inhibitors (PARPi) have shown to increase the activity of DNA damaging chemotherapeutics used in the treatment of CRC, however previous clinical trials failed to validate these results and pointed out dose-limiting toxicities that hamper the use of such combinations in unselected CRC patients. Nevertheless, in these studies little attention was paid to the mutational status of homologous recombination repair (HRR) genes. Methods: We tested the combination of the PARPi niraparib with either 5-fluorouracil, oxaliplatin or irinotecan (SN38) in a panel of 12 molecularly annotated CRC cell lines, encompassing the 4 consensus molecular subtypes (CMSs). Synergism was calculated using the Chou-Talalay method for drug interaction. A correlation between synergism and genetic alterations in genes involved in homologous recombination (HR) repair was performed. We used clonogenic assays, mice xenograft models and patient-derived 3D spheroids to validate the results. The induction of DNA damage was studied by immunofluorescence. Results: We showed that human CRC cell lines, as well as patient-derived 3D spheroids, harboring pathogenic ATM mutations are significantly vulnerable to PARPi/chemotherapy combination at low doses, regardless of consensus molecular subtypes (CMS) and microsatellite status. The strongest synergism was shown for the combination of niraparib with irinotecan, and the presence of ATM mutations was associated to a delay in the resolution of double strand breaks (DSBs) through HRR and DNA damage persistence. Conclusions: This work demonstrates that a numerically relevant subset of CRCs carrying heterozygous ATM mutations may benefit from the combination treatment with low doses of niraparib and irinotecan, suggesting a new potential approach in the treatment of ATM-mutated CRC, that deserves to be prospectively validated in clinical trials