455 research outputs found
Cropping system to limit blast disease in upland rice
Cropping system is an essential aspect to take into account to manage blast disease (caused by the fungus Magnaporthe oryzae). In addition to the selection of resistant cultivars, studies report opportunities to limit blast incidence by managing mineral amendment (N, Si, P, etc.), cultivar mixtures or other cropping system adaptations. In Madagascar, rice is the staple crop and food. Farmers traditionally grow irrigated or rainfed lowland rice wherever possible. In the mid-1980s, CIRAD and FOFIFA launched a research program for the highlands to extend upland rice growing areas in high elevation areas of the tropics. This program was consolidated with research on cropping practices that ensure the sustainability of upland rice based cropping systems in this poor and fragile environment. New varieties where obtained, adapted for rainfed cropping up to 1800 m altitude (Dzido et al., 2004). However, farmers had to face attacks of blast disease. Due to the small genetic basis of these varieties, the fungus quickly overcame resistant or tolerant lines selected by breeders. The ways of management of blast disease are very limited in a country like Madagascar where efficient solutions are often not suitable for farmers. Observations of blast epidemics in different regions made us consider the soil as a key factor for rice susceptibility. In a first experiment, we measured the potential of our very susceptible varieties to tolerate blast when cropped in different soil conditions. During two years, we transported volcanic soil from a very fertile area close to the experimentation site and where blast pressure is much lower. We observed a decrease of blast symptoms on rice cropped on that soil compared to the soil of the experimental site, both on leaves and panicles. That decrease was bound to a significant yield increase, demonstrating the importance of plant nutrition on blast incidence. Such results confirm those obtained in 1981 by Seguy et al. Our aim was then to develop cropping systems that could influence blast incidence through an improvement of soil functions. Direct seeded mulch based (DSMB) cropping systems were first used in Madagascar to limit erosion in upland areas. During 4 years, we compared blast epidemics between a traditional cropping system with ploughing each year and a DSMB cropping system, on a midsusceptible variety specific of the highlands conditions. Two fertilisation levels were also tested in these systems: zebu manure only and manure plus additional mineral fertiliser. A significant difference between the two systems was observed, both at leaf and panicle stage. Blast incidence was reduced in DSMB cropping system and, on the contrary to the traditional cropping system, the mineral fertilisation had no effect on blast in DSMB. N fertilisation is known for a long time as an essential factor in blast management. The fact that DSMB cropping system reduced the effect of N-fertilisation made us consider N as the determinant factor of the interaction between cropping system and blast incidence. The determinants of this interaction must then be explained to enable new and durable cropping systems to be developed to manage blast epidemics, in addition to cultivar improvement. This is the objectives of the starting project GARP (ANR-Systerra) which is conducted in Bolivia, Brazil, France, and Madagascar. Its aim is to quantify the interactions between cropping system, N-nutrition and blast resistance in upland rice. Hypotheses and preliminary results of the project will be presented. (Texte intégral
Quantifying the Impact of Unpredictable Generation on Market Coupling
Modeling Market Coupling using an agent-based approach, we compare two organizations: centralized versus decentralized. To perform this comparison we analytically study the impact of wind farm concentration and the uncertainty resulting from the increasing penetration of renewables on the total cost of procurement, market welfare and the ratio of renewable generation to conventional supplies. We prove that the existence and uniqueness of equilibrium depend on the number of interacting demand markets. In a decentralized organization, forecast errors heavily impact the behavior of the electrical system. Simulations show that suppliers have incentives to certify the forecast uncertainty of other markets. We analytically derive the uncertainty price that might be charged by a risk certificator depending on the required confidence level
Electron Paramagnetic Resonance Study of a Photosynthetic Microbial Mat and Comparison with Archean Cherts
International audienceOrganic radicals in artificially carbonized biomass dominated by oxygenic and non-oxygenic photosynthetic bacteria, Microcoleus chthonoplastes-like and Chloroflexus-like bacteria respectively, were studied by Electron Paramagnetic Resonance (EPR) spectroscopy. The two bacteria species were sampled in mats from a hypersaline lake. They underwent accelerated ageing by cumulative thermal treatments to induce progressive carbonization of the biological material, mimicking the natural maturation of carbonaceous material of Archean age. For thermal treatments at temperatures higher than 620 °C, a drastic increase in the EPR linewidth is observed in the carbonaceous matter from oxygenic photosynthetic bacteria and not anoxygenic photosynthetic bacteria. This selective EPR linewidth broadening reflects the presence of a catalytic element inducing formation of radical aggregates, without affecting the molecular structure or the microstructure of the organic matter, as shown by Raman spectroscopy and Transmission Electron Microscopy. For comparison, we carried out an EPR study of organic radicals in silicified carbonaceous rocks (cherts) from various localities, of different ages (0.42 to 3.5 Gyr) and having undergone various degrees of metamorphism, i.e. various degrees of natural carbonization. EPR linewidth dispersion for the most primitive samples was quite significant, pointing to a selective dipolar broadening similar to that observed for carbonized bacteria. This surprising result merits further evaluation in the light of its potential use as a marker of past bacterial metabolisms, in particular oxygenic photosynthesis, in Archean cherts
8-oxo-7,8-dihydro-2'-deoxyguanosine as a biomarker of oxidative damage in oesophageal cancer patients: lack of association with antioxidant vitamins and polymorphism of hOGG1 and GST
International audienceThe present report was designed to investigate the origins of elevated oxidative stress measured in cancer patients in our previous work related to a case-control study (17 cases, 43 controls) on oesophageal cancers. The aim was to characterize the relationship between the levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), antioxidant vitamins and genetic susceptibility
Discovery and mapping of a new expressed sequence tag-single nucleotide polymorphism and simple sequence repeat panel for large-scale genetic studies and breeding of Theobroma cacao L.
Theobroma cacao is an economically important tree of several tropical countries. Its genetic improvement is essential to provide protection against major diseases and improve chocolate quality. We discovered and mapped new expressed sequence tag-single nucleotide polymorphism (EST-SNP) and simple sequence repeat (SSR) markers and constructed a high-density genetic map. By screening 149 650 ESTs, 5246 SNPs were detected in silico, of which 1536 corresponded to genes with a putative function, while 851 had a clear polymorphic pattern across a collection of genetic resources. In addition, 409 new SSR markers were detected on the Criollo genome. Lastly, 681 new EST-SNPs and 163 new SSRs were added to the pre-existing 418 co-dominant markers to construct a large consensus genetic map. This high-density map and the set of new genetic markers identified in this study are a milestone in cocoa genomics and for marker-assisted breeding. The data are available at http://tropgenedb.cirad.fr
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